Generation and Characterization of a New Aging Skin Human Dermal Extracellular Matrix Scaffold.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-18 DOI:10.1007/7651_2024_579

Estibaliz Fernández-Carro, Jesús Ciriza

引用次数: 0

Abstract

In vitro skin aging models represent a valuable tool for the study of age-related pathologies and potential treatments. However, the currently available models do not adequately represent the complex microenvironment of the dermis since they generally focus on cutaneous cellular senescence, rather than the full range of factors that contribute to the aging process, such as structural and compositional alteration of the dermal extracellular matrix. The following protocol describes the extraction and characterization of human adult extracellular matrix scaffolds for use in in vitro aging models.

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一种新型老化皮肤人真皮细胞外基质支架的制备与表征。

体外皮肤衰老模型是研究年龄相关病理和潜在治疗方法的宝贵工具。然而，目前可用的模型并不能充分代表真皮复杂的微环境，因为它们通常关注皮肤细胞衰老，而不是导致衰老过程的各种因素，如真皮细胞外基质的结构和成分改变。以下方案描述了用于体外衰老模型的成人细胞外基质支架的提取和表征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.