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Templated 3ʹ terminal fluorescent labeling of RNA using Klenow DNA polymerase 使用 Klenow DNA 聚合酶对 RNA 进行模板化 3ʹ 末端荧光标记
IF 1.6
MethodsX Pub Date : 2024-08-28 DOI: 10.1016/j.mex.2024.102925
Mary N. Mwangi, Nathan J. Baird
{"title":"Templated 3ʹ terminal fluorescent labeling of RNA using Klenow DNA polymerase","authors":"Mary N. Mwangi,&nbsp;Nathan J. Baird","doi":"10.1016/j.mex.2024.102925","DOIUrl":"10.1016/j.mex.2024.102925","url":null,"abstract":"<div><p>A long-standing challenge in the study of RNA structure-function dynamics using fluorescence-based methods has been the precise attachment of fluorophores to structured RNA molecules. Despite significant advancements in the field, existing techniques have limitations, especially for 3ʹ end labeling of long, structured RNAs. In response to this challenge, we developed a chemo-enzymatic method that uses Klenow DNA polymerase to label RNAs. In this method:</p><ul><li><span>•</span><span><p>Klenow DNA polymerase adds an amino-modified nucleotide to the 3ʹ end of the RNA, guided by the DNA oligonucleotide template.</p></span></li><li><span>•</span><span><p>An NHS-ester dye is then conjugated to the amino-modified RNA, forming a covalent amide bond.</p></span></li><li><span>•</span><span><p>For highly structured RNAs, DNA oligonucleotides complementary to the RNA disrupt pre-existing intramolecular RNA structures.</p></span></li></ul><p>This methodological advancement enables site-specific incorporation of a single modified nucleotide at the 3′ terminus of various RNA substrates, irrespective of their length or secondary structure. The user-friendly nature of the technique, with minimal modifications required for different RNA targets, makes it readily adaptable by a broad range of researchers. This approach has the potential to significantly improve the development of functionalized RNA for various applications.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102925"},"PeriodicalIF":1.6,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003765/pdfft?md5=9972a35aac4024c0f44d902ee935e169&pid=1-s2.0-S2215016124003765-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of the new simple weight calculation (SIWEC) method in the case study in the sales channels of agricultural products 新的简单重量计算(SIWEC)方法在农产品销售渠道案例研究中的应用
IF 1.6
MethodsX Pub Date : 2024-08-22 DOI: 10.1016/j.mex.2024.102930
Adis Puška , Miroslav Nedeljković , Dragan Pamučar , Darko Božanić , Vladimir Simić
{"title":"Application of the new simple weight calculation (SIWEC) method in the case study in the sales channels of agricultural products","authors":"Adis Puška ,&nbsp;Miroslav Nedeljković ,&nbsp;Dragan Pamučar ,&nbsp;Darko Božanić ,&nbsp;Vladimir Simić","doi":"10.1016/j.mex.2024.102930","DOIUrl":"10.1016/j.mex.2024.102930","url":null,"abstract":"<div><p>In this research is presented a new method for determining the weights of criteria called simple weight calculation (SIWEC) method. The steps of this method are presented in the practical example of determining the importance of criteria for the needs of sales of agricultural products in the Semberija region. During the presentation of this method two methods are elaborated the simple SIWEC method which includes numerical ratings and the fuzzy SIWEC method which includes ratings in the form of linguistic value. In the selected example is presented how to use this method in order to determine the importance of criteria and in both cases the criterion of sales reliability is given the greatest weight. The contribution SIWEC method is reflected in its simplicity, which facilitates decision-making.</p><ul><li><span>•</span><span><p>The method presented in this research apart from others is that it uses the evaluation of the criteria by decision makers, so the criteria should not be ranked and compared, but simply evaluated.</p></span></li><li><span>•</span><span><p>Unlike similar methods, the presented method uses the adjusted steps of the method for ranking the alternatives, and decision makers are given a different importance in the decision-making.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102930"},"PeriodicalIF":1.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003819/pdfft?md5=c5eb6d1489f2737beb720b496d42cad4&pid=1-s2.0-S2215016124003819-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Data on vermicompost effect on the uptake of cadmium from soil by the roots of radish (Raphanus sativus) 蛭石堆肥对萝卜(Raphanus sativus)根从土壤中吸收镉的影响数据
IF 1.6
MethodsX Pub Date : 2024-08-22 DOI: 10.1016/j.mex.2024.102917
Ahmad Zarei , Hassan Reza Rokni
{"title":"Data on vermicompost effect on the uptake of cadmium from soil by the roots of radish (Raphanus sativus)","authors":"Ahmad Zarei ,&nbsp;Hassan Reza Rokni","doi":"10.1016/j.mex.2024.102917","DOIUrl":"10.1016/j.mex.2024.102917","url":null,"abstract":"<div><p>Cadmium is a common environmental heavy metal that is very toxic and carcinogenic for human and other flora and fauna. Therefore, this study is aimed to evaluate the fisibility of vermicompost fertilizer for cadmium uptake from soil by the root of radish (<em>Raphanus sativus</em>). For the purpose of the study, four different ratios of one case control, 1 per 1, 1 per 4, 2 per 4, 3 per 4 vermicompost fertilizer per soil with 0, 50000 and 100000 µg/L cadmium concentrations was evaluated. Cadmium in the samples was measured using an Atomic Absorption Spectroscopy (AAS). The results showed that the minimum uptake of cadmium by the plant was observed for 3 per 4 ratio of fertilizer per soil. In addition, results revealed that highest growth rate of <em>Raphanus sativus</em> roots occurred in maximum ratio of fertilizer per soil usage (3 per 4). This study showed that vermicompost as a organic fertilizer has a good ability to adsorb cadmium metal from soil. Therfore, vermicompost application can be considered as an inexpensive natural adsorbent in arable land contaminated with cadmium.</p><ul><li><span>•</span><span><p>Heavy metals are very toxic and carcinogenic to human and animals.</p></span></li><li><span>•</span><span><p>Adding organic fertilizer to the soil increases the absorption of heavy metal (cadmium) in the soil and prevents it from entering the food chain.</p></span></li><li><span>•</span><span><p>The relationship between the concentration of cadmium absorbed by the tuber of radish plant and the percentage of vermicompost added to the soil is presented.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102917"},"PeriodicalIF":1.6,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003686/pdfft?md5=3fd7289cd51846be9e17363e0a5f20d0&pid=1-s2.0-S2215016124003686-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The improved integrated Exponential Smoothing based CNN-LSTM algorithm to forecast the day ahead electricity price 基于 CNN-LSTM 算法的改进型综合指数平滑法预测未来一天的电价
IF 1.6
MethodsX Pub Date : 2024-08-20 DOI: 10.1016/j.mex.2024.102923
Kunal Shejul, R. Harikrishnan, Harshita Gupta
{"title":"The improved integrated Exponential Smoothing based CNN-LSTM algorithm to forecast the day ahead electricity price","authors":"Kunal Shejul,&nbsp;R. Harikrishnan,&nbsp;Harshita Gupta","doi":"10.1016/j.mex.2024.102923","DOIUrl":"10.1016/j.mex.2024.102923","url":null,"abstract":"<div><p>The deregulation of electricity market has led to the development of the short-term electricity market. The power generators and consumers can sell and purchase the electricity in the day ahead terms. The market clearing electricity price varies throughout the day due to the increase in the consumers bidding for electricity. Forecasting of the electricity in the day ahead market is of significance for appropriate bidding. To predict the electricity price the modified method of Exponential Smoothing-CNN-LSTM is proposed based on the time series method of Exponential Smoothing and Deep Learning methods of Convolutional Neural Network (CNN) and Long Short-Term Memory (LSTM). The dataset used for assessment of the forecasting algorithms is collected from the day ahead electricity market at the Indian Energy Exchange (IEX). The forecasting results of the Exponential Smoothing-CNN-LSTM method evaluated in terms of Mean Absolute Error (MAE) as 0.11, Root Mean Squared Error (RMSE) as 0.17 and Mean Absolute Percentage Error (MAPE) as 1.53 % indicates improved performance. The proposed algorithm can be used to forecast the time series in other domains as finance, retail, healthcare, manufacturing.</p><ul><li><span>•</span><span><p>The method of Exponential Smoothing-CNN-LSTM is proposed for forecasting the electricity price a day ahead for accurate bidding for the short-term electricity market participants.</p></span></li><li><span>•</span><span><p>The forecasting results indicate the better performance of the proposed method than the existing techniques of Exponential Smoothing, LSTM and CNN-LSTM due to the advantages of the Exponential Smoothing to extract the levels and seasonality and with the CNN-LSTM methods ability to model the complex spatial and temporal dependencies in the time series.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102923"},"PeriodicalIF":1.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003741/pdfft?md5=40eebfbb75497575f08e48b3435a4f38&pid=1-s2.0-S2215016124003741-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Composites formed by layered double hydroxides with inorganic compounds: An overview of the synthesis methods and characteristics 层状双氢氧化物与无机化合物形成的复合材料:合成方法和特点概述
IF 1.6
MethodsX Pub Date : 2024-08-20 DOI: 10.1016/j.mex.2024.102912
Franchescoli Didier Velázquez-Herrera , Yohuali Zarazua-Aguilar , Amanda S. Garzón-Pérez , Karin Monserrat Álvarez-Gómez , Geolar Fetter
{"title":"Composites formed by layered double hydroxides with inorganic compounds: An overview of the synthesis methods and characteristics","authors":"Franchescoli Didier Velázquez-Herrera ,&nbsp;Yohuali Zarazua-Aguilar ,&nbsp;Amanda S. Garzón-Pérez ,&nbsp;Karin Monserrat Álvarez-Gómez ,&nbsp;Geolar Fetter","doi":"10.1016/j.mex.2024.102912","DOIUrl":"10.1016/j.mex.2024.102912","url":null,"abstract":"<div><p>Nowadays, layered double hydroxides (LDH), sometimes referred as hydrotalcite-like compounds, have gained great attention since their composition and structure can be easily modified, so that they can be implemented in multiple fields. LDH-based composite materials based on LDH exhibit tremendously improved properties such as high specific surface area, which promotes the accessibility to a greater number of LDH active sites, considerably improving their catalytic, adsorbent and biological activities. Therefore, this review summarizes and discusses the synthesis methods of composites constituted by LDH with other inorganic compounds such as zeolites, cationic clays, hydroxyapatites, among many others, and describe the resulting characteristics of the resulting composites, emphasizing the morphology. Brief descriptions of their properties and applications are also included.</p></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102912"},"PeriodicalIF":1.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003649/pdfft?md5=70dadb039a3e7b5ffae01fbb47fb8d33&pid=1-s2.0-S2215016124003649-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural logarithm particle swarm optimization for loss reduction in an island power system 自然对数粒子群优化用于降低岛屿电力系统的损耗
IF 1.6
MethodsX Pub Date : 2024-08-20 DOI: 10.1016/j.mex.2024.102924
Alessandra F. Picanço , Antônio C. Zambroni de Souza , Andressa Pereira Oliveira
{"title":"Natural logarithm particle swarm optimization for loss reduction in an island power system","authors":"Alessandra F. Picanço ,&nbsp;Antônio C. Zambroni de Souza ,&nbsp;Andressa Pereira Oliveira","doi":"10.1016/j.mex.2024.102924","DOIUrl":"10.1016/j.mex.2024.102924","url":null,"abstract":"<div><p>In an island power system, optimizing energy management is fundamental since there are renewable sources with their limitations. This management includes the allocation and capacity of energy sources to supply the loads. In this context, optimizing losses in the system contributes to improve the efficiency of this management. This paper proposes the losses optimization and energy management in the island power system. The authors propose the Natural Logarithm Particle Swarm Optimization to solve the problem and compare it with the Attractor Point Algorithm and Evolutionary Particle Swarm Optimization. And with that, we also propose a particle initialization for the studied particle-based algorithms to guarantee convergence in radial power systems. This is because the system configuration influences the response of the algorithm convergence. These techniques were applied to the IEEE-34 unbalanced radial island system.</p><ul><li><span>•</span><span><p>Natural Logarithm Particle Swarm Optimization differs from classical PSO in that it does not calculate the velocity of the particles. Therefore, the method considers a cloud of particles with a natural logarithmic trajectory to solve the reduction of losses in a power system with a radial topology.</p></span></li><li><span>•</span><span><p>Natural Logarithmic Particle Swarm Optimization uses an initialization equation to minimize the initial estimation process, which is relevant to the convergence process.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102924"},"PeriodicalIF":1.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003753/pdfft?md5=6c32dbac50e70cbfbcfff0c783863733&pid=1-s2.0-S2215016124003753-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilizing decellularized bio-membranes to optimize histopathological embedding of small tissues 利用脱细胞生物膜优化小组织的组织病理学包埋
IF 1.6
MethodsX Pub Date : 2024-08-20 DOI: 10.1016/j.mex.2024.102919
Jinyang Li , Yanyan Zhou , Tianyou Luo , Hongzhen Mao , Lijuan Yin , Yuanfa Zhang , Tianyi He , Yang Deng , Fei Chen , Zuoyu Liang , Li Li , Lili Jiang , Ji Bao
{"title":"Utilizing decellularized bio-membranes to optimize histopathological embedding of small tissues","authors":"Jinyang Li ,&nbsp;Yanyan Zhou ,&nbsp;Tianyou Luo ,&nbsp;Hongzhen Mao ,&nbsp;Lijuan Yin ,&nbsp;Yuanfa Zhang ,&nbsp;Tianyi He ,&nbsp;Yang Deng ,&nbsp;Fei Chen ,&nbsp;Zuoyu Liang ,&nbsp;Li Li ,&nbsp;Lili Jiang ,&nbsp;Ji Bao","doi":"10.1016/j.mex.2024.102919","DOIUrl":"10.1016/j.mex.2024.102919","url":null,"abstract":"<div><p>In recent years, minimally invasive biopsy techniques have been widely used to generate small tissue samples that require processing in clinical pathology. However, small paraffin-embedded tissues are prone to loss due to their small size. To prevent the loss of small tissues, researchers have employed nonbiological embedding materials for preembedding, but this approach can lead to cumbersome experimental procedures and increase the chances of tissue loss. This study aimed to develop a convenient decellularized embedding material derived from biological membrane tissues to effectively protect small tissues from loss during paraffin embedding. This study decellularized three types of fresh animal-derived membrane tissues and selected the small intestine as the most suitable decellularized raw material through attempts at softening, comparing physical properties, and using tissue as the starting material. Subsequently, small tissues from various tissue sources were embedded, followed by H&amp;E staining, Masson staining, immunofluorescence staining, and immunohistochemical staining. The decellularized material derived from biomembrane tissues (DMBT) developed in this study can reduce the loss of small tissues without the need for preembedding, thereby shortening the embedding process. This provides a new pathological embedding tool for future laboratory and clinical research and work.</p><ul><li><span>•</span><span><p>The fat layer of the pig's small intestine is scraped off, and chemical reagents are used to defat and decellularize it.</p></span></li><li><span>•</span><span><p>Chemical reagents are used to soften and make the pig's small intestine transparent, and the decellularized pig's small intestine is dried.</p></span></li><li><span>•</span><span><p>DMBT is used for embedding and staining the biological tissue.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102919"},"PeriodicalIF":1.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003704/pdfft?md5=2526023c0543a8493e2337251d4f59f0&pid=1-s2.0-S2215016124003704-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing deep learning for detection of diabetic retinopathy in geriatric group using optical coherence tomography angiography-OCTA: A promising approach 利用深度学习,使用光学相干断层血管造影-OCTA 检测老年群体的糖尿病视网膜病变:一种前景广阔的方法
IF 1.6
MethodsX Pub Date : 2024-08-20 DOI: 10.1016/j.mex.2024.102910
Pooja Bidwai , Shilpa Gite , Biswajeet Pradhan , Harshita Gupta , Abdullah Alamri
{"title":"Harnessing deep learning for detection of diabetic retinopathy in geriatric group using optical coherence tomography angiography-OCTA: A promising approach","authors":"Pooja Bidwai ,&nbsp;Shilpa Gite ,&nbsp;Biswajeet Pradhan ,&nbsp;Harshita Gupta ,&nbsp;Abdullah Alamri","doi":"10.1016/j.mex.2024.102910","DOIUrl":"10.1016/j.mex.2024.102910","url":null,"abstract":"<div><p>The prevalence of diabetic retinopathy (DR) among the geriatric population poses significant challenges for early detection and management. Optical Coherence Tomography Angiography (OCTA) combined with Deep Learning presents a promising avenue for improving diagnostic accuracy in this vulnerable demographic. In this method, we propose an innovative approach utilizing OCTA images and Deep Learning algorithms to detect diabetic retinopathy in geriatric patients. We have collected 262 OCTA scans of 179 elderly individuals, both with and without diabetes, and trained a deep-learning model to classify retinopathy severity levels. Convolutional Neural Network (CNN) models: Inception V3, ResNet-50, ResNet50V2, VggNet-16, VggNet-19, DenseNet121, DenseNet201, EfficientNetV2B0, are trained to extract features and further classify them.</p><p>Here we demonstrate:</p><ul><li><span>•</span><span><p>The potential of OCTA and Deep Learning in enhancing geriatric eye care at the very initial stage.</p></span></li><li><span>•</span><span><p>The importance of technological advancements in addressing age-related ocular diseases and providing reliable assistance to clinicians for DR classification.</p></span></li><li><span>•</span><span><p>The efficacy of this approach in accurately identifying diabetic retinopathy stages, thereby facilitating timely interventions, and preventing vision loss in the elderly population.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102910"},"PeriodicalIF":1.6,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003625/pdfft?md5=245062457c1d67dbd7e98797f7648ea8&pid=1-s2.0-S2215016124003625-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation 优化木薯(Manihot esculenta Crantz)嫁接技术,促进其在木薯种植中的应用
IF 1.6
MethodsX Pub Date : 2024-08-19 DOI: 10.1016/j.mex.2024.102904
Frank Opoku-Agyemang , Jacqueline Naalamle Amissah , Stella Owusu-Nketia , Peter Amoako Ofori , Michitaka Notaguchi
{"title":"Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation","authors":"Frank Opoku-Agyemang ,&nbsp;Jacqueline Naalamle Amissah ,&nbsp;Stella Owusu-Nketia ,&nbsp;Peter Amoako Ofori ,&nbsp;Michitaka Notaguchi","doi":"10.1016/j.mex.2024.102904","DOIUrl":"10.1016/j.mex.2024.102904","url":null,"abstract":"<div><p>Grafting techniques have been successfully adopted to improve resistance to biotic and abiotic stresses, increase yields, fruit quality and study systemic signaling in plants. This technique has not been fully explored in cassava and there is currently no standardized grafting method for this species published especially in Africa. This is the first report on cassava grafting protocol in Africa with valuable advantages including utilizing a cost-effective and environmentally friendly wooden healing chamber. In this study, we describe an optimized cleft grafting protocol for cassava utilizing a wooden healing chamber and outline the step-by-step procedure with optimum conditions to generate a high grafting success rate. Using a top wedge grafting technique with high reproducibility and success rates, we developed a straightforward and robust grafting protocol for cassava (<em>M. esculenta</em>) cultivars. Grafting success was recorded and this protocol produced a high grafting success of 90 % and its reproducibility makes it suitable for mass production thereby addressing the need for efficient cassava propagation. This grafting protocol requires less specialized equipment and expertise making it more accessible to farmers and researchers with limited resources to promote the use of grafting for cassava growth, yield improvement and advanced studies such as systemic long-distance signaling in plants.</p><ul><li><span>•</span><span><p>Optimization of cleft grafting method obtains a high success grafting rate of cassava.</p></span></li><li><span>•</span><span><p>A wooden healing chamber provides a controlled environment for graft healing.</p></span></li><li><span>•</span><span><p>Promoting cassava grafting; a priority to produce new cultivars and explore breeding research prospects.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"13 ","pages":"Article 102904"},"PeriodicalIF":1.6,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221501612400356X/pdfft?md5=586ccde02902376b8ee749566676d624&pid=1-s2.0-S221501612400356X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methods for degrading dicloxacillin in water using inorganic peroxides and their combination with UVC- experimental and theoretical aspects 利用无机过氧化物及其与紫外线的结合在水中降解双氯西林的方法--实验和理论方面的问题
IF 1.6
MethodsX Pub Date : 2024-08-16 DOI: 10.1016/j.mex.2024.102920
Fidel Granda-Ramírez , Efraím A. Serna-Galvis , Yenny Ávila-Torres , Ricardo A. Torres-Palma , Gina Hincapié-Mejía
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