树皮甲虫和仙花甲虫与飞行条件、生殖成熟和交配相关的内部变化：解剖和评估指南

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2025-07-20 DOI:10.1016/j.mex.2025.103523

Katherine P. Bleiker , Telsa Willsey , Gregory D. Smith

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引用次数: 0

摘要

树皮甲虫和仙甲虫是一种栖息在植物上的多样化的群体。某些物种的爆发与人类价值观相竞争，并造成重大的经济和生态影响。识别树皮甲虫个体的飞行条件、生殖成熟度和交配状态有助于生物学、生理学和行为学的研究。本文详细介绍了树皮甲虫的解剖方法：解剖树皮甲虫的胸腹；提取和测量树皮甲虫的外侧中飞行肌和卵巢；提取精子并评估雌性树皮甲虫的交配状况

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Internal changes associated with flight condition, reproductive maturity, and mating in bark and ambrosia beetles: A guide for dissection and assessment

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Internal changes associated with flight condition, reproductive maturity, and mating in bark and ambrosia beetles: A guide for dissection and assessment

Bark and ambrosia beetles are a diverse group which inhabit plants. Outbreaks of some species compete with human values and cause significant economic and ecological impacts. Identifying the flight condition, reproductive maturity, and mating status of individual bark beetles can benefit biological, physiological, and behavioral studies. Here, we provide detailed methods to:

Dissect the metathorax and abdomen of bark beetles

Extract and measure the lateral medius flight muscle and ovarioles

Extract the spermatheca and assess the mating status of female bark beetles

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