Medical Microbiology and Immunology最新文献_第9页

Acinetobacter baumannii reinforces the pathogenesis by promoting IL-17 production in a mouse pneumonia model. 在小鼠肺炎模型中，鲍曼不动杆菌通过促进 IL-17 的产生来强化发病机制。

IF 5.5 3区医学

Medical Microbiology and Immunology Pub Date : 2023-02-01 Epub Date: 2022-12-03 DOI: 10.1007/s00430-022-00757-2

Yangyang Zhou, Chuanying Xiang, Ning Wang, Xiaomin Zhang, Yu Xie, Hong Yang, Gang Guo, Kaiyun Liu, Yan Li, Yun Shi

{"title":"Acinetobacter baumannii reinforces the pathogenesis by promoting IL-17 production in a mouse pneumonia model.","authors":"Yangyang Zhou, Chuanying Xiang, Ning Wang, Xiaomin Zhang, Yu Xie, Hong Yang, Gang Guo, Kaiyun Liu, Yan Li, Yun Shi","doi":"10.1007/s00430-022-00757-2","DOIUrl":"10.1007/s00430-022-00757-2","url":null,"abstract":"Interleukin-17 (IL-17) is involved in host defense against bacterial infection. Little is known about the role of IL-17 in A. baumannii-infected pneumonia. Our objective was to investigate the role of IL-17 in pulmonary A. baumannii infection in a mouse model. We infected C57BL/6 mice intra-tracheally (i.t.) with A. baumannii to establish pneumonia model and found A. baumannii infection elevated IL-17 expression in lungs. IL-17-deficient (Il17-/-) mice were resistant to pulmonary A. baumannii infection, showing improved mice survival, reduced bacteria burdens, and alleviated lung inflammation. Further, treatment of A. baumannii-infected Il17-/- mice with IL-17 exacerbated the severity of pneumonia. These data suggest a pathogenic role of IL-17 in pulmonary A. baumannii infection. Further, the infiltration and phagocytic function of neutrophils in broncho-alveolar lavage fluid were detected by flow cytometry. The results showed that Il17-/- mice had increased neutrophil infiltration and enhanced phagocytosis in neutrophils at the early time of infection. Treatment of mice with IL-17 suppressed phagocytic function of neutrophils. All data suggest that IL-17 promotes susceptibility of mice to pulmonary A. baumannii infection by suppressing neutrophil phagocytosis at early time of infection. Targeting IL-17 might be a potential therapeutic strategy in controlling the outcome of A. baumannii pneumonia.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"212 1","pages":"65-73"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10834364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance to fosfomycin is increasing and is significantly associated with extended-spectrum β-lactamase-production in urinary isolates of Escherichia coli. 对磷霉素的耐药性正在增加，并与尿中分离的大肠杆菌的广谱β-内酰胺酶产生显著相关。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-09-03 DOI: 10.1007/s00430-022-00749-2

Esther Ríos, María Del Carmen López Diaz, Esther Culebras, Iciar Rodríguez-Avial, Carmen Rodríguez-Avial

{"title":"Resistance to fosfomycin is increasing and is significantly associated with extended-spectrum β-lactamase-production in urinary isolates of Escherichia coli.","authors":"Esther Ríos, María Del Carmen López Diaz, Esther Culebras, Iciar Rodríguez-Avial, Carmen Rodríguez-Avial","doi":"10.1007/s00430-022-00749-2","DOIUrl":"https://doi.org/10.1007/s00430-022-00749-2","url":null,"abstract":"Fosfomycin has become a therapeutic option in urinary tract infections. Our objective was to evaluate the in vitro activity of fosfomycin against Escherichia coli isolated from urine samples in 2013, 2018 and 2021. We also determined a putative association between fosfomycin resistance and extended-spectrum β-lactamases (ESBL) production. Fosfomycin activity was evaluated against 7367, 8128 and 5072 Escherichia coli urinary isolates in 2013, 2018 and 2021, respectively. We compare the prevalence of fosfomycin-resistant strains among the ESBL- and non-ESBL-producing isolates. MICs of fosfomycin, cefotaxime, and cefotaxime-clavulanate were determined by a microdilution method. 302 ESBL-producers were selected to determine MICs of fosfomycin by agar dilution and genes encoding ESBLs were detected by PCR. Among the total of ESBL-producing strains, 14.3%, 20.8% and 20% were resistant to fosfomycin in 2013, 2018 and 2021, respectively, whereas fosfomycin resistance in non-ESBL producers was 3.5%, 4.05% and 5.53% for each year (P ≤ 0.001). In the 302 selected ESBL-producing isolates, CTX-M was the main ESBL (228 isolates), being 50.7% CTX-M-15. Resistance to fosfomycin among these ESBL-producing strains was associated (P = 0.049) with isolates that produced the CTX-M type. Our data show that fosfomycin resistance is increasing in Escherichia coli urinary isolates and it is related to ESBL-production. A follow-up of fosfomycin resistance is required.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"269-272"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40346616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Low rate of asymptomatic carriage and salivary immunoglobulin A response to Group A Streptococci in the healthy adult population in Finland. 芬兰健康成人对A群链球菌无症状携带和唾液免疫球蛋白A应答率低

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-09-02 DOI: 10.1007/s00430-022-00750-9

Emilia Lönnqvist, Kirsi Gröndahl-Yli-Hannuksela, Vuokko Loimaranta, Jaana Vuopio

{"title":"Low rate of asymptomatic carriage and salivary immunoglobulin A response to Group A Streptococci in the healthy adult population in Finland.","authors":"Emilia Lönnqvist, Kirsi Gröndahl-Yli-Hannuksela, Vuokko Loimaranta, Jaana Vuopio","doi":"10.1007/s00430-022-00750-9","DOIUrl":"https://doi.org/10.1007/s00430-022-00750-9","url":null,"abstract":"Streptococcus pyogenes, also called group A streptococcus (GAS), is a human pathogen causing a wide range of infections ranging from mild tonsillitis to severe, life threatening conditions such as bacteraemia, necrotizing fasciitis, and streptococcal toxic shock syndrome. GAS may also colonise the oropharynx without causing any signs of disease which is known as asymptomatic carriage. This study aims to investigate IgA responses against GAS and oral streptococci from saliva samples collected from healthy Finnish adults. In addition, asymptomatic throat GAS carriage was studied. The study participants consisted of healthy adult volunteers who provided one saliva sample, a throat swab, and a background questionnaire. Total salivary IgA, and GAS specific IgA were analysed from the saliva samples using enzyme-linked immunosorbent assays (ELISA) and the results were compared to oral streptococci specific IgA levels. Asymptomatic GAS throat carriers were identified by bacterial culture, and the isolates were emm typed. Samples from a total of 182 individuals were analysed. The median salivary IgA concentration was 62.9 µg/ml (range 17.3-649.9 µg/ml), and median GAS and oral streptococcal specific IgA concentrations 2.7 and 3.3 arbitrary units (AU, range 1.4-7.4 AU and 1.6-12.0 AU), respectively. Three individuals with asymptomatic GAS throat carriage were identified.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"261-267"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40341766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological imbalance in microcephalic children with congenital Zika virus syndrome. 先天性寨卡病毒综合征小头畸形儿童的免疫失调。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-07-20 DOI: 10.1007/s00430-022-00746-5

Amanda Costa Ayres Salmeron, Wallace Pitanga Bezerra, Rafaela Lúcia Lopes de Souza, Luanderson Cardoso Pereira, Lícia Maria do Nascimento, Anna Cláudia Calvielli Castelo Branco, Luiza Emilia Cavalcanti Simas, Valéria Azevedo de Almeida, Pedro Henrique de Souza Palmeira, Christiane Medeiros Bezerra, Paulo Marcos Matta Guedes, Maria Notomi Sato, Valéria Soraya de Farias Sales, Reginaldo Antônio de Oliveira Freitas Júnior, Tatjana de Souza Lima Keesen, Manuela Sales Lima Nascimento

{"title":"Immunological imbalance in microcephalic children with congenital Zika virus syndrome.","authors":"Amanda Costa Ayres Salmeron, Wallace Pitanga Bezerra, Rafaela Lúcia Lopes de Souza, Luanderson Cardoso Pereira, Lícia Maria do Nascimento, Anna Cláudia Calvielli Castelo Branco, Luiza Emilia Cavalcanti Simas, Valéria Azevedo de Almeida, Pedro Henrique de Souza Palmeira, Christiane Medeiros Bezerra, Paulo Marcos Matta Guedes, Maria Notomi Sato, Valéria Soraya de Farias Sales, Reginaldo Antônio de Oliveira Freitas Júnior, Tatjana de Souza Lima Keesen, Manuela Sales Lima Nascimento","doi":"10.1007/s00430-022-00746-5","DOIUrl":"https://doi.org/10.1007/s00430-022-00746-5","url":null,"abstract":"Microcephalic children due congenital Zika virus syndrome (CZS) present neurological symptoms already well described. However, several other alterations can also be observed. Here, we aimed to evaluate the immune system of microcephaly CZS children. We showed that these patients have enlarged thymus, spleen and cervical lymph nodes, analysed by ultrasound and compared to the reference values for healthy children. In the periphery, they have an increase in eosinophil count and morphological alterations as hypersegmented neutrophils and atypical lymphocytes, even in the absence of urinary tract infections, parasitological infections or other current symptomatic infections. Microcephalic children due CZS also have high levels of IFN-γ, IL-2, IL-4, IL-5 and type I IFNs, compared to healthy controls. In addition, this population showed a deficient cellular immune memory as demonstrated by the low reactivity to the tuberculin skin test even though they had been vaccinated with BCG less than 2 years before the challenge with the PPD. Together, our data demonstrate for the first time that CZS can cause alterations in primary and secondary lymphoid organs and also alters the morphology and functionality of the immune system cells, which broadens the spectrum of CZS symptoms. This knowledge may assist the development of specific therapeutic and more efficient vaccination schemes for this population of patients.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"219-235"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40538518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Human cytomegalovirus (HCMV) long-term shedding and HCMV-specific immune response in pregnant women with primary HCMV infection. 人巨细胞病毒(HCMV)在原发性HCMV感染孕妇中的长期脱落和HCMV特异性免疫反应

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-08-12 DOI: 10.1007/s00430-022-00747-4

C Fornara, F Zavaglio, M Furione, A Sarasini, P d'Angelo, A Arossa, A Spinillo, D Lilleri, F Baldanti

{"title":"Human cytomegalovirus (HCMV) long-term shedding and HCMV-specific immune response in pregnant women with primary HCMV infection.","authors":"C Fornara, F Zavaglio, M Furione, A Sarasini, P d'Angelo, A Arossa, A Spinillo, D Lilleri, F Baldanti","doi":"10.1007/s00430-022-00747-4","DOIUrl":"https://doi.org/10.1007/s00430-022-00747-4","url":null,"abstract":"Human cytomegalovirus (HCMV) shedding has been extensively investigated in newborns and in young children, however, much less is known about it in immunocompetent adults. Shedding of HCMV was investigated in saliva, vaginal secretions and urine of pregnant women experiencing primary infection along with the development of the HCMV-specific immune response. Thirty-three pregnant women shed HCMV DNA in peripheral biological fluids at least until one year after onset of infection, while in blood HCMV DNA was cleared earlier. Significantly higher levels of viral load were found in vaginal secretions compared to saliva and urine. All subjects examined two years after the onset of infection showed a high avidity index, with IgM persisting in 36% of women. Viral load in blood was directly correlated with levels of HCMV-specific IgM and inversely correlated with levels of IgG specific for the pentameric complex gH/gL/pUL128L; in addition, viral load in blood was inversely correlated with percentage of HCMV-specific CD4+ and CD8+ expressing IL-7R (long-term memory, LTM) while viral load in biological fluids was inversely correlated with percentage of HCMV-specific CD4+ and CD8+ effector memory RA+(TEMRA). In conclusion, viral shedding during primary infection in pregnancy persists in peripheral biological fluids for at least one year and the development of both antibodies (including those directed toward the pentameric complex) and memory T cells are associated with viral clearance.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"249-260"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40624151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Changes in circulating TCF1- and GARP-associated regulatory T cell subsets reflect the clinical status of patients with chronic HBV infection. 循环TCF1-和garp相关调节性T细胞亚群的变化反映了慢性HBV感染患者的临床状态。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-08-11 DOI: 10.1007/s00430-022-00748-3

Ayibaota Bahabayi, Xingyue Zeng, Bulidierxin Tuerhanbayi, Yangyang Zhang, Ainizati Hasimu, Siyu Guo, Tianci Liu, Mohan Zheng, Xiayidan Alimu, Chen Liu

{"title":"Changes in circulating TCF1- and GARP-associated regulatory T cell subsets reflect the clinical status of patients with chronic HBV infection.","authors":"Ayibaota Bahabayi, Xingyue Zeng, Bulidierxin Tuerhanbayi, Yangyang Zhang, Ainizati Hasimu, Siyu Guo, Tianci Liu, Mohan Zheng, Xiayidan Alimu, Chen Liu","doi":"10.1007/s00430-022-00748-3","DOIUrl":"https://doi.org/10.1007/s00430-022-00748-3","url":null,"abstract":"This study aimed to clarify the expression changes and clinical significance of regulatory T (Treg) cells and follicular regulatory T (TFR) cell subsets divided by glycoprotein A repetitions predominant protein (GARP) and T cell factor 1(TCF1) in peripheral blood of patients with chronic HBV infection. The peripheral blood of 26 chronic hepatitis B (CHB) patients, 27 inactive HBsAg carriers and 32 healthy controls were collected and GARP + percentages in Treg and TFR cells were analyzed by flow cytometry. In addition, Treg and TFR cell subsets sorted by CD62L and TCF1 were analyzed and compared. Correlation analyses were performed between Treg and TFR cell subpopulations and clinical parameters as well as cytokine concentrations, including IL-21, IL-10 and TGF-β1 in plasma. Circulating Treg and TFR levels were elevated in CHB patients. Moreover, GARP and TCF1 were up-regulated in circulating Treg and TFR cells of CHB patients. TCF1 + CD62L- Treg cells were increased while TCF1-CD62L + Treg cells were decreased in CHB patients. TCF1 + CD62L- and TCF1-CD62L- TFR cells were increased while TCF1 + CD62L + TFR cells were decreased in CHB patients. TCF1 + CD62L- Treg cells were positively correlated with HBV DNA, ALT and plasma IL-10, while TCF1 + CD62L + TFR cells were negatively correlated with HBV DNA, HBeAg, HBsAg, ALT, AST, T-BIL and positively correlated with plasma IL-21. Treg and TFR subsets sorted by TCF1, CD62L and GARP were changed in CHB patients. Changes in Treg and TFR functional subsets are associated with antiviral immunity in CHB patients.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"237-247"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40687435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Obituary Prof. Ulrich Vogel, MD 1964-2022: a scientific mentor and expert in infection prevention and control. 讣告乌尔里希-沃格尔教授，医学博士，1964-2022 年：科学导师和感染预防与控制专家。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-11-03 DOI: 10.1007/s00430-022-00751-8

Oliver Kurzai, Heike Claus, Thien-Trí Lâm

引用次数: 0

Child type 1 diabetes associated with mother vaginal bacteriome and mycobiome 儿童1型糖尿病与母亲阴道细菌群和真菌群的关系

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-14 DOI: 10.1007/s00430-022-00741-w

A. Ruotsalainen, M. Tejesvi, P. Vänni, M. Suokas, P. Tossavainen, A. Pirttilä, A. Talvensaari‐Mattila, R. Nissi

引用次数: 5

Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples 自采干斑标本血液和唾液中SARS-CoV-2抗体水平

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-13 DOI: 10.1007/s00430-022-00740-x

L. Lahdentausta, Anne S Kivimäki, Lotta-Maria A. H. Oksanen, Marika Tallgren, Sampo A. Oksanen, E. Sanmark, A. Salminen, A. Geneid, Mikko Sairanen, S. Paju, K. Saksela, P. Pussinen, M. Pietiäinen

引用次数: 5

Inflammatory cytokine profile and T cell responses in African tick bite fever patients 非洲蜱叮咬热患者的炎症细胞因子谱和T细胞反应

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-01 DOI: 10.1007/s00430-022-00738-5

J. Rauch, J. Jochum, Philip Eisermann, Jana Gisbrecht, Katrin Völker, F. Hunstig, U. Mehlhoop, B. Muntau, D. Tappe

引用次数: 0