Medical Microbiology and Immunology最新文献_第8页

Resistance to fosfomycin is increasing and is significantly associated with extended-spectrum β-lactamase-production in urinary isolates of Escherichia coli. 对磷霉素的耐药性正在增加，并与尿中分离的大肠杆菌的广谱β-内酰胺酶产生显著相关。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-09-03 DOI: 10.1007/s00430-022-00749-2

Esther Ríos, María Del Carmen López Diaz, Esther Culebras, Iciar Rodríguez-Avial, Carmen Rodríguez-Avial

{"title":"Resistance to fosfomycin is increasing and is significantly associated with extended-spectrum β-lactamase-production in urinary isolates of Escherichia coli.","authors":"Esther Ríos, María Del Carmen López Diaz, Esther Culebras, Iciar Rodríguez-Avial, Carmen Rodríguez-Avial","doi":"10.1007/s00430-022-00749-2","DOIUrl":"https://doi.org/10.1007/s00430-022-00749-2","url":null,"abstract":"Fosfomycin has become a therapeutic option in urinary tract infections. Our objective was to evaluate the in vitro activity of fosfomycin against Escherichia coli isolated from urine samples in 2013, 2018 and 2021. We also determined a putative association between fosfomycin resistance and extended-spectrum β-lactamases (ESBL) production. Fosfomycin activity was evaluated against 7367, 8128 and 5072 Escherichia coli urinary isolates in 2013, 2018 and 2021, respectively. We compare the prevalence of fosfomycin-resistant strains among the ESBL- and non-ESBL-producing isolates. MICs of fosfomycin, cefotaxime, and cefotaxime-clavulanate were determined by a microdilution method. 302 ESBL-producers were selected to determine MICs of fosfomycin by agar dilution and genes encoding ESBLs were detected by PCR. Among the total of ESBL-producing strains, 14.3%, 20.8% and 20% were resistant to fosfomycin in 2013, 2018 and 2021, respectively, whereas fosfomycin resistance in non-ESBL producers was 3.5%, 4.05% and 5.53% for each year (P ≤ 0.001). In the 302 selected ESBL-producing isolates, CTX-M was the main ESBL (228 isolates), being 50.7% CTX-M-15. Resistance to fosfomycin among these ESBL-producing strains was associated (P = 0.049) with isolates that produced the CTX-M type. Our data show that fosfomycin resistance is increasing in Escherichia coli urinary isolates and it is related to ESBL-production. A follow-up of fosfomycin resistance is required.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"269-272"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40346616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Low rate of asymptomatic carriage and salivary immunoglobulin A response to Group A Streptococci in the healthy adult population in Finland. 芬兰健康成人对A群链球菌无症状携带和唾液免疫球蛋白A应答率低

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-09-02 DOI: 10.1007/s00430-022-00750-9

Emilia Lönnqvist, Kirsi Gröndahl-Yli-Hannuksela, Vuokko Loimaranta, Jaana Vuopio

{"title":"Low rate of asymptomatic carriage and salivary immunoglobulin A response to Group A Streptococci in the healthy adult population in Finland.","authors":"Emilia Lönnqvist, Kirsi Gröndahl-Yli-Hannuksela, Vuokko Loimaranta, Jaana Vuopio","doi":"10.1007/s00430-022-00750-9","DOIUrl":"https://doi.org/10.1007/s00430-022-00750-9","url":null,"abstract":"Streptococcus pyogenes, also called group A streptococcus (GAS), is a human pathogen causing a wide range of infections ranging from mild tonsillitis to severe, life threatening conditions such as bacteraemia, necrotizing fasciitis, and streptococcal toxic shock syndrome. GAS may also colonise the oropharynx without causing any signs of disease which is known as asymptomatic carriage. This study aims to investigate IgA responses against GAS and oral streptococci from saliva samples collected from healthy Finnish adults. In addition, asymptomatic throat GAS carriage was studied. The study participants consisted of healthy adult volunteers who provided one saliva sample, a throat swab, and a background questionnaire. Total salivary IgA, and GAS specific IgA were analysed from the saliva samples using enzyme-linked immunosorbent assays (ELISA) and the results were compared to oral streptococci specific IgA levels. Asymptomatic GAS throat carriers were identified by bacterial culture, and the isolates were emm typed. Samples from a total of 182 individuals were analysed. The median salivary IgA concentration was 62.9 µg/ml (range 17.3-649.9 µg/ml), and median GAS and oral streptococcal specific IgA concentrations 2.7 and 3.3 arbitrary units (AU, range 1.4-7.4 AU and 1.6-12.0 AU), respectively. Three individuals with asymptomatic GAS throat carriage were identified.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"261-267"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40341766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunological imbalance in microcephalic children with congenital Zika virus syndrome. 先天性寨卡病毒综合征小头畸形儿童的免疫失调。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-07-20 DOI: 10.1007/s00430-022-00746-5

Amanda Costa Ayres Salmeron, Wallace Pitanga Bezerra, Rafaela Lúcia Lopes de Souza, Luanderson Cardoso Pereira, Lícia Maria do Nascimento, Anna Cláudia Calvielli Castelo Branco, Luiza Emilia Cavalcanti Simas, Valéria Azevedo de Almeida, Pedro Henrique de Souza Palmeira, Christiane Medeiros Bezerra, Paulo Marcos Matta Guedes, Maria Notomi Sato, Valéria Soraya de Farias Sales, Reginaldo Antônio de Oliveira Freitas Júnior, Tatjana de Souza Lima Keesen, Manuela Sales Lima Nascimento

{"title":"Immunological imbalance in microcephalic children with congenital Zika virus syndrome.","authors":"Amanda Costa Ayres Salmeron, Wallace Pitanga Bezerra, Rafaela Lúcia Lopes de Souza, Luanderson Cardoso Pereira, Lícia Maria do Nascimento, Anna Cláudia Calvielli Castelo Branco, Luiza Emilia Cavalcanti Simas, Valéria Azevedo de Almeida, Pedro Henrique de Souza Palmeira, Christiane Medeiros Bezerra, Paulo Marcos Matta Guedes, Maria Notomi Sato, Valéria Soraya de Farias Sales, Reginaldo Antônio de Oliveira Freitas Júnior, Tatjana de Souza Lima Keesen, Manuela Sales Lima Nascimento","doi":"10.1007/s00430-022-00746-5","DOIUrl":"https://doi.org/10.1007/s00430-022-00746-5","url":null,"abstract":"Microcephalic children due congenital Zika virus syndrome (CZS) present neurological symptoms already well described. However, several other alterations can also be observed. Here, we aimed to evaluate the immune system of microcephaly CZS children. We showed that these patients have enlarged thymus, spleen and cervical lymph nodes, analysed by ultrasound and compared to the reference values for healthy children. In the periphery, they have an increase in eosinophil count and morphological alterations as hypersegmented neutrophils and atypical lymphocytes, even in the absence of urinary tract infections, parasitological infections or other current symptomatic infections. Microcephalic children due CZS also have high levels of IFN-γ, IL-2, IL-4, IL-5 and type I IFNs, compared to healthy controls. In addition, this population showed a deficient cellular immune memory as demonstrated by the low reactivity to the tuberculin skin test even though they had been vaccinated with BCG less than 2 years before the challenge with the PPD. Together, our data demonstrate for the first time that CZS can cause alterations in primary and secondary lymphoid organs and also alters the morphology and functionality of the immune system cells, which broadens the spectrum of CZS symptoms. This knowledge may assist the development of specific therapeutic and more efficient vaccination schemes for this population of patients.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"219-235"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40538518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Human cytomegalovirus (HCMV) long-term shedding and HCMV-specific immune response in pregnant women with primary HCMV infection. 人巨细胞病毒(HCMV)在原发性HCMV感染孕妇中的长期脱落和HCMV特异性免疫反应

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-08-12 DOI: 10.1007/s00430-022-00747-4

C Fornara, F Zavaglio, M Furione, A Sarasini, P d'Angelo, A Arossa, A Spinillo, D Lilleri, F Baldanti

{"title":"Human cytomegalovirus (HCMV) long-term shedding and HCMV-specific immune response in pregnant women with primary HCMV infection.","authors":"C Fornara, F Zavaglio, M Furione, A Sarasini, P d'Angelo, A Arossa, A Spinillo, D Lilleri, F Baldanti","doi":"10.1007/s00430-022-00747-4","DOIUrl":"https://doi.org/10.1007/s00430-022-00747-4","url":null,"abstract":"Human cytomegalovirus (HCMV) shedding has been extensively investigated in newborns and in young children, however, much less is known about it in immunocompetent adults. Shedding of HCMV was investigated in saliva, vaginal secretions and urine of pregnant women experiencing primary infection along with the development of the HCMV-specific immune response. Thirty-three pregnant women shed HCMV DNA in peripheral biological fluids at least until one year after onset of infection, while in blood HCMV DNA was cleared earlier. Significantly higher levels of viral load were found in vaginal secretions compared to saliva and urine. All subjects examined two years after the onset of infection showed a high avidity index, with IgM persisting in 36% of women. Viral load in blood was directly correlated with levels of HCMV-specific IgM and inversely correlated with levels of IgG specific for the pentameric complex gH/gL/pUL128L; in addition, viral load in blood was inversely correlated with percentage of HCMV-specific CD4+ and CD8+ expressing IL-7R (long-term memory, LTM) while viral load in biological fluids was inversely correlated with percentage of HCMV-specific CD4+ and CD8+ effector memory RA+(TEMRA). In conclusion, viral shedding during primary infection in pregnancy persists in peripheral biological fluids for at least one year and the development of both antibodies (including those directed toward the pentameric complex) and memory T cells are associated with viral clearance.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"249-260"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40624151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Changes in circulating TCF1- and GARP-associated regulatory T cell subsets reflect the clinical status of patients with chronic HBV infection. 循环TCF1-和garp相关调节性T细胞亚群的变化反映了慢性HBV感染患者的临床状态。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-12-01 Epub Date: 2022-08-11 DOI: 10.1007/s00430-022-00748-3

Ayibaota Bahabayi, Xingyue Zeng, Bulidierxin Tuerhanbayi, Yangyang Zhang, Ainizati Hasimu, Siyu Guo, Tianci Liu, Mohan Zheng, Xiayidan Alimu, Chen Liu

{"title":"Changes in circulating TCF1- and GARP-associated regulatory T cell subsets reflect the clinical status of patients with chronic HBV infection.","authors":"Ayibaota Bahabayi, Xingyue Zeng, Bulidierxin Tuerhanbayi, Yangyang Zhang, Ainizati Hasimu, Siyu Guo, Tianci Liu, Mohan Zheng, Xiayidan Alimu, Chen Liu","doi":"10.1007/s00430-022-00748-3","DOIUrl":"https://doi.org/10.1007/s00430-022-00748-3","url":null,"abstract":"This study aimed to clarify the expression changes and clinical significance of regulatory T (Treg) cells and follicular regulatory T (TFR) cell subsets divided by glycoprotein A repetitions predominant protein (GARP) and T cell factor 1(TCF1) in peripheral blood of patients with chronic HBV infection. The peripheral blood of 26 chronic hepatitis B (CHB) patients, 27 inactive HBsAg carriers and 32 healthy controls were collected and GARP + percentages in Treg and TFR cells were analyzed by flow cytometry. In addition, Treg and TFR cell subsets sorted by CD62L and TCF1 were analyzed and compared. Correlation analyses were performed between Treg and TFR cell subpopulations and clinical parameters as well as cytokine concentrations, including IL-21, IL-10 and TGF-β1 in plasma. Circulating Treg and TFR levels were elevated in CHB patients. Moreover, GARP and TCF1 were up-regulated in circulating Treg and TFR cells of CHB patients. TCF1 + CD62L- Treg cells were increased while TCF1-CD62L + Treg cells were decreased in CHB patients. TCF1 + CD62L- and TCF1-CD62L- TFR cells were increased while TCF1 + CD62L + TFR cells were decreased in CHB patients. TCF1 + CD62L- Treg cells were positively correlated with HBV DNA, ALT and plasma IL-10, while TCF1 + CD62L + TFR cells were negatively correlated with HBV DNA, HBeAg, HBsAg, ALT, AST, T-BIL and positively correlated with plasma IL-21. Treg and TFR subsets sorted by TCF1, CD62L and GARP were changed in CHB patients. Changes in Treg and TFR functional subsets are associated with antiviral immunity in CHB patients.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 5-6","pages":"237-247"},"PeriodicalIF":5.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40687435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Obituary Prof. Ulrich Vogel, MD 1964-2022: a scientific mentor and expert in infection prevention and control. 讣告乌尔里希-沃格尔教授，医学博士，1964-2022 年：科学导师和感染预防与控制专家。

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-11-03 DOI: 10.1007/s00430-022-00751-8

Oliver Kurzai, Heike Claus, Thien-Trí Lâm

引用次数: 0

Child type 1 diabetes associated with mother vaginal bacteriome and mycobiome 儿童1型糖尿病与母亲阴道细菌群和真菌群的关系

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-14 DOI: 10.1007/s00430-022-00741-w

A. Ruotsalainen, M. Tejesvi, P. Vänni, M. Suokas, P. Tossavainen, A. Pirttilä, A. Talvensaari‐Mattila, R. Nissi

引用次数: 5

Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples 自采干斑标本血液和唾液中SARS-CoV-2抗体水平

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-13 DOI: 10.1007/s00430-022-00740-x

L. Lahdentausta, Anne S Kivimäki, Lotta-Maria A. H. Oksanen, Marika Tallgren, Sampo A. Oksanen, E. Sanmark, A. Salminen, A. Geneid, Mikko Sairanen, S. Paju, K. Saksela, P. Pussinen, M. Pietiäinen

引用次数: 5

Inflammatory cytokine profile and T cell responses in African tick bite fever patients 非洲蜱叮咬热患者的炎症细胞因子谱和T细胞反应

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-01 DOI: 10.1007/s00430-022-00738-5

J. Rauch, J. Jochum, Philip Eisermann, Jana Gisbrecht, Katrin Völker, F. Hunstig, U. Mehlhoop, B. Muntau, D. Tappe

引用次数: 0

Genetic variability in minor capsid protein (L2 gene) of human papillomavirus type 16 among Indian women. 印度妇女16型人乳头瘤病毒次要衣壳蛋白(L2基因)的遗传变异

IF 5.4 3区医学

Medical Microbiology and Immunology Pub Date : 2022-06-01 DOI: 10.1007/s00430-022-00739-4

Arati Mane, Sanket Limaye, Linata Patil, Urmila Kulkarni-Kale

{"title":"Genetic variability in minor capsid protein (L2 gene) of human papillomavirus type 16 among Indian women.","authors":"Arati Mane, Sanket Limaye, Linata Patil, Urmila Kulkarni-Kale","doi":"10.1007/s00430-022-00739-4","DOIUrl":"https://doi.org/10.1007/s00430-022-00739-4","url":null,"abstract":"Human papillomavirus type 16 (HPV-16) is the predominant genotype worldwide associated with invasive cervical cancer and hence remains as the focus for diagnostic development and vaccine research. L2, the minor capsid protein forms the packaging unit for the HPV genome along with the L1 protein and is primarily associated with transport of genomic DNA to the nucleus. Unlike L1, L2 is known to elicit cross-neutralizing antibodies and thus becomes a suitable candidate for pan-HPV prophylactic vaccine development. In the present study, a total of 148 cervical HPV-16 isolates from Indian women were analyzed by PCR-directed sequencing, phylogenetic analysis and in silico immunoinformatics tools to determine the L2 variations that may impact the immune response and oncogenesis. Ninety-one SNPs translating to 35 non-synonymous amino acid substitutions were observed, of these 16 substitutions are reported in the Indian isolates for the first time. T245A, L266F, S378V and S384A substitutions were significantly associated with high-grade cervical neoplastic status. Multiple substitutions were observed in samples from high-grade cervical neoplastic status as compared to those from normal cervical status (p = 0.027), specifically from the D3 sub-lineage. It was observed that substitution T85A was part of both, B and T cell epitopes recognized by MHC-I molecules; T245A was common to B and T cell epitopes recognized by MHC-II molecules and S122P/A was common to the region recognized by both MHC-I and MHC-II molecules. These findings reporting L2 protein substitutions have implications on cervical oncogenesis and design of next-generation L2-based HPV vaccines.","PeriodicalId":18369,"journal":{"name":"Medical Microbiology and Immunology","volume":"211 2-3","pages":"153-160"},"PeriodicalIF":5.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10598174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1