Medical and Veterinary Entomology最新文献

{"title":"Influence of insular conditions on wing phenotypic variation in two dominant mosquito vectors, Aedes albopictus and Armigeres subalbatus (Diptera: Culicidae), in the border archipelagos of Thailand","authors":"Sedthapong Laojun,&nbsp;Narin Sontigun,&nbsp;Tanawat Chaiphongpachara","doi":"10.1111/mve.12722","DOIUrl":"10.1111/mve.12722","url":null,"abstract":"<p>Insects geographically separated into island and mainland populations often exhibit phenotypic variations, a phenomenon known as insular conditions. These conditions can lead to rapid evolutionary changes that affect the morphological characteristics of mosquito vectors. Nevertheless, studies that specifically examine phenotype differences between island and mainland mosquito populations have been limited. In this study, wing variation in size and shape was investigated using the geometric morphometric (GM) technique in two dominant mosquito vectors, <i>Aedes albopictus</i> and <i>Armigeres subalbatus</i>, in the Ranong and Trat archipelagos of Thailand. Significant differences in average wing centroid size (CS) were found in 6 out of 15 population pairs for <i>Ae. albopictus</i> (<i>p</i> &lt; 0.05) and in 5 population pairs for <i>Ar. subalbatus</i> (<i>p</i> &lt; 0.05). After removing the allometric effect, canonical variate analyses (CVA) based on wing shape analysis revealed overlap across all populations for both <i>Ae. albopictus</i> and <i>Ar. subalbatus</i>. However, the statistical analysis indicated that <i>Ar. subalbatus</i> exhibited wing shape differences across all populations (<i>p</i> &lt; 0.05), and most <i>Ae. albopictus</i> populations also displayed distinct wing shapes (<i>p</i> &lt; 0.05), except for the populations from Chang Island and the mainland of Ranong, which showed no significant differences (<i>p</i> &gt; 0.05). These findings enhance our understanding of mosquito adaptability in island regions and provide valuable data for the surveillance and monitoring of vector evolution.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"349-360"},"PeriodicalIF":1.6,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140629128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometric morphometrics to differentiate species and explore seasonal variation in three Mansonia species (Diptera: Culicidae) in central Thailand and their association with meteorological factors","authors":"Sedthapong Laojun,&nbsp;Tanasak Changbunjong,&nbsp;Arina Abdulloh,&nbsp;Tanawat Chaiphongpachara","doi":"10.1111/mve.12720","DOIUrl":"10.1111/mve.12720","url":null,"abstract":"<p><i>Mansonia</i> mosquito species are recognised as a significant vector of human pathogens, primarily transmitting the filarial nematode, <i>Brugia malayi</i>. In central Thailand, the three most prevalent <i>Mansonia</i> species are <i>Mansonia annulifera</i>, <i>Mansonia indiana</i> and <i>Mansonia uniformis</i>. This study explored the influence of seasonal changes on the phenotypic variation of these <i>Mansonia</i> species in central Thailand using the geometric morphometrics (GM). To ensure accurate species identification, we integrated GM techniques with DNA barcoding, examining distinctions in both phenotype and genotype among the species. The intraspecific genetic divergence ranged from 0.00% to 1.69%, whereas the interspecific genetic divergence ranged from 10.52% to 16.36%. The clear distinction between intra- and interspecific distances demonstrated the presence of a barcoding gap, confirming the successful differentiation of the three <i>Mansonia</i> mosquito species through DNA barcoding. Similarly, the interspecies GM assessment for classifying <i>Mansonia</i> species demonstrated a high degree of accuracy, with an overall performance of 98.12%. Exploring seasonal variation in the three <i>Mansonia</i> species revealed wing variations across different seasons, and pronounced variations appearing in the cool season. Regarding their association with meteorological factors, <i>Ma. annulifera</i> and <i>Ma. uniformis</i> showed significant positive correlations with temperature (<i>p</i> &lt; 0.05), and <i>Ma. uniformis</i> also displayed a significant negative correlation with atmospheric pressure (<i>p</i> &lt; 0.05). The insights from this study will deepen our understanding of the adaptive patterns of <i>Mansonia</i> mosquitoes in Thailand's central region, paving the way for enhanced disease surveillance related to these vectors.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"325-340"},"PeriodicalIF":1.6,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140577696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenology and habitat associations of the invasive Asian longhorned tick from Ohio, USA","authors":"Andreas Eleftheriou,&nbsp;Benjamin Zeiger,&nbsp;Jazmin Jennings,&nbsp;Risa Pesapane","doi":"10.1111/mve.12719","DOIUrl":"10.1111/mve.12719","url":null,"abstract":"<p>Geographically expanding and invading ticks are a global concern. The Asian longhorned tick (ALT, <i>Haemaphysalis longicornis</i>) was introduced to the mid-Atlantic US between 2010 and 2017 and recently invaded Ohio, an inland state. To date, ALTs in the US have been associated with livestock exsanguination and transmission of the agent of bovine theileriosis. To inform management, studies describing tick ecology and epidemiology of associated disease agents are critical. In this study, we described phenology, habitat and host associations, and tested for agents of medical and veterinary concern at the site of the first known established ALT population in Ohio, where pesticide treatment was applied in early fall 2021. In spring–fall 2022, we sampled wildlife (small mammals) and collected ticks from forest, edge, and grassland habitats. We also opportunistically sampled harvested white-tailed deer at nearby processing stations and fresh wildlife carcasses found near roads. Field-collected ALTs were tested for five agents using real-time PCR. We found that ALT nymphs emerged in June, followed by adults, and concluded with larvae in the fall. ALTs were detected in all habitats but not in wildlife. We also found a 4.88% (2/41) prevalence of <i>Anaplasma phagocytophilum</i> across ALT adults and nymphs. Host and habitat associations were similar to other studies in the eastern United States, but two potential differences in phenology were identified. Whether ALTs will acquire more endemic disease agents requires further investigations. Our findings provide the first evidence regarding ALT life history from the Midwest region of the United States and can inform exposure risk and guide integrated management.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"314-324"},"PeriodicalIF":1.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140577428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrapuparial development of Sarcophaga (Liopygia) ruficornis (Diptera: Sarcophagidae), a species of medical-veterinary and forensic importance, under laboratory conditions\u0000 Desenvolvimento intrapuparial de Sarcophaga (Liopygia) ruficornis (Diptera: Sarcophagidae), uma espécie de importância médico-veterinária e forense, em condições de laboratório","authors":"Marina Lopes Duarte,&nbsp;Tatiane Aparecida Nascimento,&nbsp;Margareth Maria Carvalho de Queiroz","doi":"10.1111/mve.12718","DOIUrl":"10.1111/mve.12718","url":null,"abstract":"<p><i>Sarcophaga</i> (=<i>Parasarcophaga</i>) (<i>Liopygia</i>) <i>ruficornis</i> (Fabricius, 1794) is a species of medical–veterinary and forensic importance, as its immatures cause myiasis in humans and animals and colonize carcasses and cadavers. Therefore, investigations into the biology and morphology of this species, with a particular focus on pupae that constitute ≥50% of the developmental period for the immatures, are pertinent. Although there are biological and morphological studies of pupae, the intrapuparial development at different temperatures has not yet been analysed. Therefore, the present study aimed to describe how temperature (22, 27 and 32 ± 1°C) affects the development and morphology of <i>S</i>. (<i>L</i>.) <i>ruficornis</i> pupae at 60 ± 10% relative humidity and a 12-h photoperiod. Ten pupae were collected, euthanized and fixed every 4 h from pupariation until 24 h and every 8 h until the emergence of the first adult. Emergence occurred at 440, 272 and 232 h at 22, 27 and 32°C, with 590, 380 and 330 pupae, respectively. The highest mortality occurred at 32°C. Eight periods were defined, and sex was determined in pharate adult stage; in addition, 40 key morphological characteristics to estimate pupal age were presented. These findings can serve as a basis for studies on the biology and morphology of the pupa of <i>S</i>. (<i>L</i>.) <i>ruficornis</i>, particularly in bionomics, control and forensics, helping researchers and experts.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"303-313"},"PeriodicalIF":1.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140577891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The internal transcribed spacer 1 sequence polymorphism brings updates to tsetse species distribution in the northern Cameroon: Importance in planning efficient vector control","authors":"Steve Feudjio Soffack,&nbsp;Tito Tresor Melachio Tanekou,&nbsp;Oumarou Farikou,&nbsp;Ginette Irma Kame Ngasse,&nbsp;Mureille Carole Tchami Mbagnia,&nbsp;Murielle Wondji,&nbsp;Charles S. Wondji,&nbsp;Adly M. M. Abd-Alla,&nbsp;Anne Geiger,&nbsp;Gustave Simo,&nbsp;Flobert Njiokou","doi":"10.1111/mve.12717","DOIUrl":"10.1111/mve.12717","url":null,"abstract":"<p>Vector control remains one of the best strategies to prevent the transmission of trypanosome infections in humans and livestock and, thus, a good way to achieve the elimination of human African trypanosomiasis and animal African trypanosomiasis. A key prerequisite for the success of any vector control strategy is the accurate identification and correct mapping of tsetse species. In this work, we updated the tsetse fly species identification and distribution in many geographical areas in Cameroon. Tsetse flies were captured from six localities in Cameroon, and their species were morphologically identified. Thereafter, DNA was extracted from legs of each tsetse fly and the length polymorphism of internal transcribed spacer-1 (ITS1) region of each fly was investigated using PCR. ITS1 DNA fragments of each tsetse species were sequenced. The sequences obtained were analysed and compared to those available in GenBank. This enabled to confirm/infirm results of the morphologic identification and then, to establish the phylogenetic relationships between tsetse species. Morphologic features allowed to clearly distinguish all the tsetse species captured in the South Region of Cameroon, that is, <i>Glossina palpalis palpalis, G. pallicera</i>, <i>G. caliginea</i> and <i>G. nigrofusca</i>. In the northern area, <i>G. morsitans submorsitans</i> could also be distinguished from <i>G. palpalis palpalis</i>, <i>G. tachinoides</i> and <i>G. fuscipes</i>, but these three later could not be distinguished with routine morphological characters. The ITS1 length polymorphism was high among most of the studied species and allowed to identify the following similar species with a single PCR, that is, <i>G. palpalis palpalis</i> with 241 or 242 bp and <i>G. tachinoides</i> with 221 or 222 bp, <i>G. fuscipes</i> with 236 or 237 bp. We also updated the old distribution of tsetse species in the areas assessed, highlighting the presence of <i>G. palpalis palpalis</i> instead of <i>G. fuscipes</i> in Mbakaou, or in sympatry with <i>G. morsitans submorsitans</i> in Dodeo (northern Cameroon). This study confirms the presence of <i>G. palpalis palpalis</i> in the Adamawa Region of Cameroon. It highlights the limits of using morphological criteria to differentiate some tsetse species. Molecular tools based on the polymorphism of ITS1 of tsetse flies can differentiate tsetse species through a simple PCR before downstream analyses or vector control planning.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"216-226"},"PeriodicalIF":1.9,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First biochemical and behavioural analysis of the response of the scorpion Urophonius brachycentrus (Thorell: 1876) upon exposure to an organophosphate","authors":"Carlos F. Garcia,&nbsp;Andrés Ojanguren,&nbsp;Analía Seoane,&nbsp;Hernan Iuri,&nbsp;Rocio Gambaro,&nbsp;Gabriel Molina,&nbsp;Aldana Laino","doi":"10.1111/mve.12716","DOIUrl":"10.1111/mve.12716","url":null,"abstract":"<p>Scorpionism is an increasing public health problem in the world. Although no specific methodology or product is currently available for the control of those arachnids, the use of insecticides could be an effective tool. Chlorpyrifos is one of the insecticides used, but to date, whether scorpions recognise surfaces with that insecticide and how it affects their physiology and/or biochemistry is unknown. In the present study, we observed that scorpions recognise surfaces with 0.51 and 8.59 μg/cm² of chlorpyrifos and avoid those areas. The 0.51 μg/cm² concentration produced a decrease in acetylcholinesterase and an increase in catalase, superoxide dismutase and glutathione S-transferase, whereas the 8.59 μg/cm² concentration evoked a decrease in acetylcholinesterase and an increase in catalase and glutathione S-transferase. Using the comet assay, we observed that the insecticide at 0.17, 0.51 and 8.59 μg/cm² caused DNA damage. Finally, we found that the insecticide does not generate significant variations in glutathione peroxidase, glutathione reductase, the amount of protein or lipid peroxidation. The present results offer a comprehensive understanding of how scorpions respond, both at the biochemical and behavioural levels, when exposed to insecticides.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"291-302"},"PeriodicalIF":1.6,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular identification of Haemonchus contortus (Nematoda: Trichostrongylidae), a blood-sucking gastric nematode of artiodactyles, in the ground beetle Carabus granulatus (Coleoptera: Carabidae)","authors":"Joanna Werszko,&nbsp;Konrad Wilamowski,&nbsp;Olga Kraszewska,&nbsp;Sławomir Bakier,&nbsp;Anna Maria Pyziel","doi":"10.1111/mve.12715","DOIUrl":"10.1111/mve.12715","url":null,"abstract":"<p>Among gastrointestinal nematodes, <i>Haemonchus contortus</i> (Rudolphi) Cobb (order Strongylidae; family Trichostrongylidae) is one of pathogenic and economic importance in domestic and wild ruminants, including the European bison, <i>Bison bonasus</i> Linnaeus (order Cetartiodactyla; family Bovidae); a species on the International Union for Conservation of Nature's Red List of Threatened Species. <i>Carabus granulatus</i> Linnaeus (order Coleoptera; family Carabidae) is one of the most prevalent species of ground beetle, inhabiting a wide range of terrestrial ecosystems in Poland. Twenty-six ground beetles of this species inhabiting the Białowieża Primeval Forest in eastern Poland were screened for the presence of DNA of pathogenic gastrointestinal nematodes of ruminants. Extracted DNA was sequenced and compared to reference sequences. In six insects, the presence of <i>H. contortus</i> DNA was detected. The obtained nucleotide sequences were homologous to each other and to the majority of the published DNA sequences of <i>H. contortus</i> isolates. The sequences were also identical to a sequence of <i>H. contortus</i> isolated from European bison in Poland. The study provides the first molecular evidence of the presence of <i>H. contortus</i> DNA in <i>C. granulatus</i>. The finding suggests that ground beetles may play a role in the transmission dynamics of this parasite.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"361-365"},"PeriodicalIF":1.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible role of haematophagous flies in the incidence of bovine teat papillomatosis","authors":"Mitsuhiro Iwasa,&nbsp;Yumeno Shido,&nbsp;Shinichi Hatama","doi":"10.1111/mve.12714","DOIUrl":"10.1111/mve.12714","url":null,"abstract":"<p>The relationship between the incidence of bovine teat papillomatosis and the activity of haematophagous flies was investigated in Japan. A total of 15,737 flies consisting of 33 species were collected by dry ice-baited mosquito net (DMN) trap and a sweep net from udders of cattle. <i>Simulium aokii</i> (Takahasi) of Simuliidae (black flies) was the predominant species, followed by <i>S. tobetsuense</i> Ono and <i>S. iwatense</i> (Shiraki). <i>Simulium aokii</i> had the highest peak in October, followed by September. Numbers of blood spots from the bites per teat in nulliparous cattle were significantly correlated with numbers of <i>S. aokii</i> collected by DMN trap. Numbers of teats with warts and spots of blood from the bites per teat were significantly more abundant in anterior teats than posterior teats. The average incidence of teat papillomatosis in nulliparous cattle was significantly higher than that in parous cattle, and the highest incidence by month was in May, followed by April. Although bovine papillomavirus (BPV) DNA was not detected in flies examined, the presence of black flies and blood spots from their bites were associated with subsequent high incidence of growing warts. In particular, it would pay to give attention to species such as <i>S. aokii</i> that severely attack udders in the present locality. Further investigations for the detection of BPV DNA from flies parasitizing on teats are needed.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"280-290"},"PeriodicalIF":1.6,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140158463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Mayaro virus vector competence of the mosquito Aedes aegypti (Linnaeus, 1762) populations in Argentine using dose–response assays","authors":"Mauricio Daniel Beranek,&nbsp;Octavio Giayetto,&nbsp;Sylvia Fischer,&nbsp;Adrián Diaz","doi":"10.1111/mve.12712","DOIUrl":"10.1111/mve.12712","url":null,"abstract":"<p>Mayaro virus (MAYV; <i>Alphavirus</i>: <i>Togaviridae</i>) is an emerging pathogen in Latin America, causing fever and polyarthritis. Sporadic outbreaks of MAYV have occurred in the region, with reported human cases being imported to Europe and North America. Although primarily a risk for those residing in the Amazon basin's tropical forests, recent reports highlight that urbanization would increase the risk of MAYV transmission in Latin America. Urban emergence depends on human susceptibility and the ability of mosquitos like <i>Aedes aegypti</i>  (Linnaeus, 1762) (Diptera: Culicidae) to transmit MAYV. Despite the absence of active MAYV transmission in Argentine, the risk of introduction is substantial due to human movement and the presence of <i>Ae. aegypti</i> in the region. This study aimed to evaluate the susceptibility of different Argentine <i>Ae. aegypti</i> populations to MAYV genotype L (MAYV-L) using dose–response assays and determine barriers to virus infection, dissemination and transmission. Immature mosquito stages were collected in Buenos Aires, Córdoba and Rosario cities. Female <i>Ae. aegypti</i> (F2) were orally infected by feeding on five concentrations of MAYV-L, ranging from 1.0 to 6.0 log₁₀ PFU/mL. Abdomens, legs and saliva were analysed using viral plaque assays. Results revealed that MAYV-L between infection and dissemination were associated with viral doses rather than the population origin. Infection rates varied between 3% and 65%, with a 50% infectious dose &gt;5.5 log₁₀ PFU/mL. Dissemination occurred at 39%, with a 50% dissemination dose of ~6.0 log₁₀ PFU/mL. Dissemination among infected mosquitoes ranged from 60% to 86%, and transmission from disseminated mosquitoes ranged from 11% to 20%. Argentine <i>Ae. aegypti</i> populations exhibited a need for higher viral doses of MAYV-L than those typically found in humans to become infected. In addition, only a small proportion of infected mosquitoes were capable of transmitting the virus. Understanding MAYV transmission in urban areas is crucial for public health interventions.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"234-243"},"PeriodicalIF":1.9,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140136964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of deltamethrin exposure on the cytochrome P450 monooxygenases of Aedes albopictus (Skuse) larvae from a dengue-endemic region of northern part of West Bengal, India","authors":"Prapti Das,&nbsp;Subhajit Das,&nbsp;Abhirup Saha,&nbsp;Debayan Raha,&nbsp;Dhiraj Saha","doi":"10.1111/mve.12713","DOIUrl":"10.1111/mve.12713","url":null,"abstract":"<p><i>Aedes albopictus</i> is highly prevalent in the northern part of West Bengal and is considered to be responsible for the recent dengue outbreaks in this region. Control of this vector is largely relied on the use of synthetic pyrethroids, which can lead to the development of resistance. In the present study, larvae of three wild <i>Ae. albopictus</i> populations from the dengue-endemic regions were screened for deltamethrin resistance, and the role of cytochrome P450 monooxygenases (CYPs) was investigated in deltamethrin exposed and unexposed larvae. Two populations were incipient resistant, and one population was completely resistant against deltamethrin. Monooxygenase titration assay revealed the involvement of CYPs in deltamethrin resistance along with an induction effect of deltamethrin exposure. Gene expression studies revealed differential expression of five CYP6 family genes, <i>CYP6A8</i>, <i>CYP6P12</i>, <i>CYP6A14</i>, <i>CYP6N3</i> and <i>CYP6N6</i>, with high constitutive expression of <i>CYP6A8</i> and <i>CYP6P12</i> in all the populations before and after deltamethrin exposure. From these findings, it was evident that CYPs play an important role in the development of deltamethrin resistance in the <i>Ae. albopictus</i> populations in this region.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"269-279"},"PeriodicalIF":1.6,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}