Diversity of lice and flea- and lice-borne pathogens in free-ranging dogs in Uzbekistan.

Abstract

Vector-borne diseases pose a significant threat to human and animal health worldwide, with arthropods, including fleas and lice, acting as key vectors for transmitting various pathogens. In Uzbekistan, where millions of domestic dogs coexist with humans, the diversity of vector-borne pathogens carried by ectoparasites remains largely unexplored. This study aimed to investigate the diversity and prevalence of lice and pathogens carried by fleas and lice collected from domestic dogs in Uzbekistan. Ectoparasites were collected from 77 dogs across five regions and subjected to morphological identification and molecular analysis. The 47 lice collected were identified as Trichodectes canis Fabricius, 1780 (Psocodea: Trichodectidae), Linognathus africanus Kellogg and Paine, 1911 (Psocodea: Linognathidae) and Bovicola bovis Linnaeus, 1758 (Phthiraptera: Trichodectidae), alongside with 197 fleas. Notably, L. africanus was detected for the first time on dogs worldwide. Molecular screening detected Rickettsia spp. in 27.9% fleas and 44.7% lice, Dipylidium caninum Linnaeus, 1758 (Cyclophyllidea: Dipylidiidae) in 1.5% of fleas and Bartonella spp. in 0.5% of fleas. Sequencing confirmed Rickettsia asembonensisMaina et al., 2016 (Rickettsiales: Rickettsiaceae), Rickettsia helvetica Beati et al., 1993 (Rickettsiales: Rickettsiaceae), Rickettsia monacensis Simser et al., 2019 (Rickettsiales: Rickettsiaceae), D. caninum and Bartonella clarridgeiae Lawson and Collins, 1996 (Hyphomicrobiales: Bartonellaceae). These findings highlight the potential role of dogs as reservoirs for vector-borne pathogens and emphasises the need for further research to inform effective disease control strategies.