Medical and Veterinary Entomology最新文献

{"title":"Host preference and human blood index of Phlebotomus orientalis, an exophilic sand fly vector of visceral leishmaniasis in eastern Sudan","authors":"Tayseer Jibreel,&nbsp;Altayeb Khogali,&nbsp;Maribel Jiménez,&nbsp;Adeel Raiyed,&nbsp;Osman Dakein,&nbsp;Bashir Alsharif,&nbsp;Noteila M. Khalid,&nbsp;Omran F. Osman,&nbsp;Bakri Y. M. Nour,&nbsp;Gamal Hassan Mohamed,&nbsp;Ricardo Molina,&nbsp;Ana Vidal-López,&nbsp;Ramón Díaz-Regañón,&nbsp;Margriet den Boer,&nbsp;Jorge Alvar,&nbsp;Orin Courtenay,&nbsp;Dia-Eldin Elnaiem","doi":"10.1111/mve.12683","DOIUrl":"10.1111/mve.12683","url":null,"abstract":"<p>Visceral leishmaniasis (VL, kala azar), caused by <i>Leishmania donovani</i>, transmitted by <i>Phlebotomus orientalis</i>, is a serious systemic disease that causes high morbidity and mortality rates in Sudan and other parts of East Africa and the world. Despite progress in understanding the epidemiology of the disease in East Africa, little is known about the host preference of <i>P. orientalis</i> in kala azar endemic villages of Sudan, which have some of the highest VL incidence rates in the world. The present study used host choice experiments and blood-meal identification approaches to determine the host preference of <i>P. orientalis</i> in kala azar endemic villages in Gedarif state, eastern Sudan. In the host choice experiment, tent traps were used to compare the attractiveness of cows, donkeys, sheep and goats for host-seeking <i>P. orientalis</i>. In the blood-meal identification study, blood-fed <i>P. orientalis</i> females, captured inside houses and peri-domestic habitats, were subjected to molecular typing using cytochrome <i>b</i> gene (cyt <i>b</i>) amplification and sequence analysis. Cows and donkeys were the most attractive to blood-seeking <i>P. orientalis</i>, followed by goats. Similarly, the blood-meal analysis of <i>P. orientalis</i> showed that the vector preferentially feeds on cows, followed by donkeys, humans and goats. The human blood index of <i>P. orientalis</i> was 19.4% (42/216), indicating a high zoophilic habit of the vector, both inside and outside the houses. Although the order of host preference varied by location, it was clear that cows are the most preferred host of <i>P. orientalis</i> in the area. Results are discussed in relation to the role of domestic/livestock animals in VL zoopotentiation and zooprophylaxis. Inference is made on the potential impact of insecticide treatment of cows in control of the vector and the transmission of VL in Sudan and other parts of East Africa.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"782-792"},"PeriodicalIF":1.9,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12683","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9935391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning analysis of wing venation patterns accurately identifies Sarcophagidae, Calliphoridae and Muscidae fly species","authors":"Min Hao Ling,&nbsp;Tania Ivorra,&nbsp;Chong Chin Heo,&nbsp;April Hari Wardhana,&nbsp;Martin Jonathan Richard Hall,&nbsp;Siew Hwa Tan,&nbsp;Zulqarnain Mohamed,&nbsp;Tsung Fei Khang","doi":"10.1111/mve.12682","DOIUrl":"10.1111/mve.12682","url":null,"abstract":"<p>In medical, veterinary and forensic entomology, the ease and affordability of image data acquisition have resulted in whole-image analysis becoming an invaluable approach for species identification. Krawtchouk moment invariants are a classical mathematical transformation that can extract local features from an image, thus allowing subtle species-specific biological variations to be accentuated for subsequent analyses. We extracted Krawtchouk moment invariant features from binarised wing images of 759 male fly specimens from the Calliphoridae, Sarcophagidae and Muscidae families (13 species and a species variant). Subsequently, we trained the Generalized, Unbiased, Interaction Detection and Estimation random forests classifier using linear discriminants derived from these features and inferred the species identity of specimens from the test samples. Fivefold cross-validation results show a 98.56 ± 0.38% (standard error) mean identification accuracy at the family level and a 91.04 ± 1.33% mean identification accuracy at the species level. The mean <i>F</i>1-score of 0.89 ± 0.02 reflects good balance of precision and recall properties of the model. The present study consolidates findings from previous small pilot studies of the usefulness of wing venation patterns for inferring species identities. Thus, the stage is set for the development of a mature data analytic ecosystem for routine computer image-based identification of fly species that are of medical, veterinary and forensic importance.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"767-781"},"PeriodicalIF":1.9,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Culicoides caucoliberensis in Spain: Exploring molecular phylogeny, host-feeding behaviour and avian haemosporidian parasites","authors":"Mikel A. González,&nbsp;Daniel Bravo-Barriga,&nbsp;Júlia López-Mercadal,&nbsp;Miguel Ángel Miranda,&nbsp;Rafael Gutiérrez-López,&nbsp;Carlos Barceló","doi":"10.1111/mve.12679","DOIUrl":"10.1111/mve.12679","url":null,"abstract":"<p><i>Culicoides</i> biting midges (Diptera: Ceratopogonidae) are vectors of pathogens that affect wildlife and livestock. Understanding the composition and distribution of vector species is crucial for implementing control strategies and preventing the spread of infectious diseases. This study provides a morphological and molecular characterisation of <i>Culicoides caucoliberensis</i>, which represents the first record for Spain, increasing the number of <i>Culicoides</i> species in the country to 85. A total of 213 specimens were collected using Onderstepoort-ultraviolet down-draught light traps on a rocky coastline in the Balearic Islands during two sampling periods in 2022. Phylogenetic analysis showed that <i>C. caucoliberensis</i> forms a monophyletic cluster within the Maritimus group. Host preferences were determined for the first time and showed propensity to feed on the European shag (<i>Phalacrocorax aristotelis</i>). The vector role of <i>C. caucoliberensis</i> for haemosporidian transmission remains unclear since molecular detection of Haemosporidians (<i>Haemoproteus</i> and <i>Plasmodium</i>) was negative for all the pools of parous and engorged females analysed. This study emphasises the importance of conducting entomofauna studies in lesser-known Mediterranean islet landscapes and highlights the need for research on vectors within the One Health framework.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"871-877"},"PeriodicalIF":1.9,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9817213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Worldwide comparison between the potential distribution of Rhipicephalus microplus (Acari: Ixodidae) under climate change scenarios","authors":"M. B. Perez-Martinez,&nbsp;D. A. Moo-Llanes,&nbsp;C. N. Ibarra-Cerdeña,&nbsp;D. Romero-Salas,&nbsp;A. Cruz-Romero,&nbsp;K. M. López-Hernández,&nbsp;M. Aguilar-Dominguez","doi":"10.1111/mve.12680","DOIUrl":"10.1111/mve.12680","url":null,"abstract":"<p>The cattle tick <i>Rhipicephalus microplus</i> (Acari: Ixodidae) has demonstrated its ability to increase its distribution raising spatially its importance as a vector for zoonotic hemotropic pathogens. In this study, a global ecological niche model of <i>R. microplus</i> was built in different scenarios using Representative Concentration Pathway (RCP), Socio-Economic Pathway (SSP), and a climatic dataset to determine where the species could establish itself and thus affect the variability in the presentation of the hemotropic diseases they transmit. America, Africa and Oceania showed a higher probability for the presence of <i>R. microplus</i> in contrast to some countries in Europe and Asia in the ecological niche for the current period (1970–2000), but with the climate change, there was an increase in the ratio between the geographic range preserved between the RCP and SSP scenarios obtaining the greatest gain in the interplay of RCP4.5-SSP245. Our results allow to determine future changes in the distribution of the cattle tick according to the increase in environmental temperature and socio-economic development influenced by human development activities and trends; this work explores the possibility of designing integral maps between the vector and specific diseases.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"745-753"},"PeriodicalIF":1.9,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9754655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative expression of key genes involved in nucleic acids methylation in Anopheles gambiae sensu stricto","authors":"Oswald Y. Djihinto,&nbsp;Dario Meacci,&nbsp;Adandé A. Medjigodo,&nbsp;Federica Bernardini,&nbsp;Luc S. Djogbénou","doi":"10.1111/mve.12681","DOIUrl":"10.1111/mve.12681","url":null,"abstract":"In vertebrates, enzymes responsible for DNA methylation, one of the epigenetic mechanisms, are encoded by genes falling into the cytosine methyltransferases genes family (Dnmt1, Dnmt3a,b and Dnmt3L). However, in Diptera, only the methyltransferase Dnmt2 was found, suggesting that DNA methylation might act differently for species in this order. Moreover, genes involved in epigenetic dynamics, such as Ten‐eleven Translocation dioxygenases (TET) and Methyl‐CpG‐binding domain (MBDs), present in vertebrates, might play a role in insects. This work aimed at investigating nucleic acids methylation in the malaria vector Anopheles gambiae (Diptera: Culicidae) by analysing the expression of Dnmt2, TET2 and MBDs genes using quantitative real‐time polymerase chain reaction (qRT‐PCR) at pre‐immature stages and in reproductive tissues of adult mosquitoes. In addition, the effect of two DNA methylation inhibitors on larval survival was evaluated. The qPCR results showed an overall low expression of Dnmt2 at all developmental stages and in adult reproductive tissues. In contrast, MBD and TET2 showed an overall higher expression. In adult mosquito reproductive tissues, the expression level of the three genes in males' testes was significantly higher than that in females' ovaries. The chemical treatments did not affect larval survival. The findings suggest that mechanisms other than DNA methylation underlie epigenetic regulation in An. gambiae.","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"754-766"},"PeriodicalIF":1.9,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9761737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and spatial distribution of Japanese encephalitis virus vector species in Cambodia","authors":"Rutaiwan Dusadeepong,&nbsp;Pierre-Olivier Maquart,&nbsp;Mallorie Hide,&nbsp;Sebastien Boyer","doi":"10.1111/mve.12678","DOIUrl":"10.1111/mve.12678","url":null,"abstract":"<p>In Southeast Asia, despite the use of Japanese encephalitis vaccines and vaccination coverage, Japanese encephalitis (JE) transmission is still a major public health issue. The main vectors of this virus are mosquitoes from the genus <i>Culex,</i> which diversity and density are important in Southeast Asia. The main vector species of Japanese encephalitis virus (JEV) in Cambodia belong to the Vishnui subgroup. However, their morphological identification solely based on the adult stage remains challenging, making their segregation and detection difficult.</p><p>In order to identify and describe the distribution of the three main JEV vector species in Cambodia, namely <i>Culex vishnui</i>, <i>Cx. pseudovishnui</i> and <i>Cx. tritaeniorhynchus</i>, mosquito samplings were carried out throughout the country in different environments. Phylogenetic analysis of the cytochrome c oxidase subunit I (coI) gene using maximum-likelihood tree with ultrafast bootstrap and phylogeographic analysis were performed. The three main <i>Culex</i> species are phylogenetically separated, and represent two distinct clades, one with <i>Cx. tritaeniorhynchus</i> and the second with <i>Cx. vishnui</i> and <i>Cx. pseudovishnui</i>, the latter appearing as a subgroup of <i>Cx. vishnui</i>. The phylogeographic analysis shows a distribution of the Vishnui subgroup on the entire Cambodian territory with an overlapped distribution areas leading to a sympatric distribution of these species. The three JEV vector species are geographically well-defined with a strong presence of <i>Cx. pseudovishnui</i> in the forest. Combined with the presence of <i>Cx. tritaeniorhynchus</i> and C<i>x. vishnui</i> in rural, peri-urban, and urban areas, the presence of JEV-competent vectors is widespread in Cambodia.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"737-744"},"PeriodicalIF":1.9,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9751350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of RNA interference for the management of arthropod pests in livestock farms\u0000 L'uso di RNA interferente per il controllo degli artropodi infestanti negli allevamenti","authors":"Valeria Bonina,&nbsp;Salvatore Arpaia","doi":"10.1111/mve.12677","DOIUrl":"10.1111/mve.12677","url":null,"abstract":"<p>Pest management in farm animals is an important action to contain economic damage to livestock production and prevent transmission of severe diseases to the stock. The use of chemical insecticides is still the most common approach followed by farmers; however, avoiding possible toxic effects on animals is a fundamental task for pest control measures compatible with animal well-being. Moreover, legal constraints and insurgence of resistance by target species to the available insecticidal compounds are increasingly complicating farmers' operations. Alternatives to chemical pesticides have been explored with some promising results in the area of biological control or the use of natural products as sprays. The application of RNA interference techniques has enabled the production of new means of pest control in agriculture, and it is opening a promising avenue for controlling arthropod pests of livestock. Transcript depletion of specific target genes of the recipient organisms is based on the action of double-strand RNAs (dsRNA) capable of impairing the production of fundamental proteins. Their mode of action, based on the specific recognition of short genomic sequences, is expected to be highly selective towards non-target organisms potentially exposed; in addition, there are physical and chemical barriers to dsRNA uptake by mammalian cells that render these products practically innocuous for higher animals. Summarising existing literature on gene silencing for main taxa of arthropod pests of livestock (Acarina, Diptera, Blattoidea), this review explores the perspectives of practical applications of dsRNA-based pesticides against the main pests of farm animals. Knowledge gaps are summarised to stimulate additional research in this area.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"631-646"},"PeriodicalIF":1.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12677","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9803177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein abundance in the midgut of wild tsetse flies (Glossina palpalis palpalis) naturally infected by Trypanosoma congolense s.l.","authors":"Jean Marc Tsagmo,&nbsp;Flobert Njiokou,&nbsp;Alexis Dziedziech,&nbsp;Valerie Rofidal,&nbsp;Sonia Hem,&nbsp;Anne Geiger","doi":"10.1111/mve.12676","DOIUrl":"10.1111/mve.12676","url":null,"abstract":"Tsetse flies (Glossina spp.) are major vectors of African trypanosomes, causing either Human or Animal African Trypanosomiasis (HAT or AAT). Several approaches have been developed to control the disease, among which is the anti‐vector Sterile Insect Technique. Another approach to anti‐vector strategies could consist of controlling the fly's vector competence through hitherto unidentified regulatory factors (genes, proteins, biological pathways, etc.). The present work aims to evaluate the protein abundance in the midgut of wild tsetse flies (Glossina palpalis palpalis) naturally infected by Trypanosoma congolense s.l. Infected and non‐infected flies were sampled in two HAT/AAT foci in Southern Cameroon. After dissection, the proteomes from the guts of parasite‐infected flies were compared to that of uninfected flies to identify quantitative and/or qualitative changes associated with infection. Among the proteins with increased abundance were fructose‐1,6‐biphosphatase, membrane trafficking proteins, death proteins (or apoptosis proteins) and SERPINs (inhibitor of serine proteases, enzymes considered as trypanosome virulence factors) that displayed the highest increased abundance. The present study, together with previous proteomic and transcriptomic studies on the secretome of trypanosomes from tsetse fly gut extracts, provides data to be explored in further investigations on, for example, mammal host immunisation or on fly vector competence modification via para‐transgenic approaches.","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"723-736"},"PeriodicalIF":1.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9689300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Competitive interactions with Aedes albopictus alter the nutrient content of Aedes aegypti","authors":"Hunter Deerman,&nbsp;Donald A. Yee","doi":"10.1111/mve.12675","DOIUrl":"10.1111/mve.12675","url":null,"abstract":"<p>Competition is often cited as a central force that affects the distribution and performance of organisms. Ecological stoichiometry is the balance of elements within animal bodies that can be affected by resource acquisition and processing, as well as by intra- or interspecific interactions. Though relatively underexplored for mosquitoes, stoichiometry may provide a wealth of information linking ecological interactions to body nutrient content, and potentially on to pathogen transmission. Detritus, which often varies in nutrient content, forms the base of the food web within the small aquatic habitats occupied by larval mosquitoes, and detrital nutrient content can alter mosquito growth, survival, and population growth. The invasive mosquitoes <i>Aedes albopictus</i> (Diptera: Culicidae) and <i>Aedes aegypti</i> (Diptera: Culicidae) interact as larvae in aquatic systems, often altering their adult populations. Herein, we investigated how different detritus combinations as well as how intra- and interspecific densities of <i>Ae. albopictus</i> and <i>Ae. aegypti</i> would affect coexistence; we also measured how nutrient composition (carbon and nitrogen) and stoichiometry (C:N) of adults would vary with those interactions. <i>Ae. albopictus</i> survival, population growth, and stoichiometry were not affected by intra- or interspecific competition; nutrient values did vary with detritus ratios. However, <i>Ae. aegypti</i> nutrient content and stoichiometry and survival were negatively affected within the lowest nutrient environments in the presence of <i>Ae. albopictus</i>, but in the highest nutrient environments, both species showed high survival rates and population growth. This is the first study to show that adult mosquito body nutrients can be altered by interspecific interactions, and as nutrient content in adults has been linked to pathogen transmission, it provides a novel role of competition in affecting disease dynamics.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"715-722"},"PeriodicalIF":1.9,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Rickettsia spp. and Anaplasma ovis in Melophagus ovinus from southern Xinjiang, China","authors":"Si-Ang Li,&nbsp;Liu Zhang,&nbsp;Zheng Li,&nbsp;Hao-Nan Song,&nbsp;Ze-Wei Que,&nbsp;Si-Yu Zhao,&nbsp;Ying-Ying Li,&nbsp;Yu-Ling Guo,&nbsp;Jun-Yuan Wu","doi":"10.1111/mve.12673","DOIUrl":"10.1111/mve.12673","url":null,"abstract":"<p><i>Melophagus ovinus</i> is a hematophagous insect that is distributed worldwide and plays a crucial role in transmitting disease-causing pathogens. From June 2021 to March 2022, a total of 370 <i>M. ovinus</i> were collected from 11 sampling points in southern Xinjiang, China. The specimens were identified using morphological and molecular analyses. <i>Rickettsia</i> spp. and <i>Anaplasma ovis</i> were detected from all the samples using seven <i>Rickettsia</i>-specific genetic markers and the <i>msp</i>-4 gene of <i>A. ovis</i>. Approximately 11% of the <i>M. ovinus</i> specimens were positive for <i>Rickettsia</i> spp., and <i>Candidatus</i> Rickettsia barbariae was the most predominant species (35/41; 85.4%), while <i>R. massiliae</i> was least prevalent (6/41; 14.6%). Approximately 10.5% (39/370) of the <i>M. ovinus</i> specimens were positive for <i>A. ovis</i> of genotype III, which was co-detected with <i>Candidatus</i> R. barbariae in <i>M. ovinus</i> (3/370; 0.8%). To the best of our knowledge, this is the first report of the detection of <i>R. massiliae</i> and <i>Candidatus</i> R. barbariae in <i>M. ovinus</i> globally. The detection and control of insect-borne diseases originating from <i>M. ovinus</i> should be strengthened in southern Xinjiang, an area important to animal husbandry and production.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"37 4","pages":"865-870"},"PeriodicalIF":1.9,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9723070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}