Medical and Veterinary Entomology最新文献

{"title":"Intrapuparial development of Sarcophaga (Liopygia) ruficornis (Diptera: Sarcophagidae), a species of medical-veterinary and forensic importance, under laboratory conditions\u0000 Desenvolvimento intrapuparial de Sarcophaga (Liopygia) ruficornis (Diptera: Sarcophagidae), uma espécie de importância médico-veterinária e forense, em condições de laboratório","authors":"Marina Lopes Duarte,&nbsp;Tatiane Aparecida Nascimento,&nbsp;Margareth Maria Carvalho de Queiroz","doi":"10.1111/mve.12718","DOIUrl":"10.1111/mve.12718","url":null,"abstract":"<p><i>Sarcophaga</i> (=<i>Parasarcophaga</i>) (<i>Liopygia</i>) <i>ruficornis</i> (Fabricius, 1794) is a species of medical–veterinary and forensic importance, as its immatures cause myiasis in humans and animals and colonize carcasses and cadavers. Therefore, investigations into the biology and morphology of this species, with a particular focus on pupae that constitute ≥50% of the developmental period for the immatures, are pertinent. Although there are biological and morphological studies of pupae, the intrapuparial development at different temperatures has not yet been analysed. Therefore, the present study aimed to describe how temperature (22, 27 and 32 ± 1°C) affects the development and morphology of <i>S</i>. (<i>L</i>.) <i>ruficornis</i> pupae at 60 ± 10% relative humidity and a 12-h photoperiod. Ten pupae were collected, euthanized and fixed every 4 h from pupariation until 24 h and every 8 h until the emergence of the first adult. Emergence occurred at 440, 272 and 232 h at 22, 27 and 32°C, with 590, 380 and 330 pupae, respectively. The highest mortality occurred at 32°C. Eight periods were defined, and sex was determined in pharate adult stage; in addition, 40 key morphological characteristics to estimate pupal age were presented. These findings can serve as a basis for studies on the biology and morphology of the pupa of <i>S</i>. (<i>L</i>.) <i>ruficornis</i>, particularly in bionomics, control and forensics, helping researchers and experts.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"303-313"},"PeriodicalIF":1.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140577891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The internal transcribed spacer 1 sequence polymorphism brings updates to tsetse species distribution in the northern Cameroon: Importance in planning efficient vector control","authors":"Steve Feudjio Soffack,&nbsp;Tito Tresor Melachio Tanekou,&nbsp;Oumarou Farikou,&nbsp;Ginette Irma Kame Ngasse,&nbsp;Mureille Carole Tchami Mbagnia,&nbsp;Murielle Wondji,&nbsp;Charles S. Wondji,&nbsp;Adly M. M. Abd-Alla,&nbsp;Anne Geiger,&nbsp;Gustave Simo,&nbsp;Flobert Njiokou","doi":"10.1111/mve.12717","DOIUrl":"10.1111/mve.12717","url":null,"abstract":"<p>Vector control remains one of the best strategies to prevent the transmission of trypanosome infections in humans and livestock and, thus, a good way to achieve the elimination of human African trypanosomiasis and animal African trypanosomiasis. A key prerequisite for the success of any vector control strategy is the accurate identification and correct mapping of tsetse species. In this work, we updated the tsetse fly species identification and distribution in many geographical areas in Cameroon. Tsetse flies were captured from six localities in Cameroon, and their species were morphologically identified. Thereafter, DNA was extracted from legs of each tsetse fly and the length polymorphism of internal transcribed spacer-1 (ITS1) region of each fly was investigated using PCR. ITS1 DNA fragments of each tsetse species were sequenced. The sequences obtained were analysed and compared to those available in GenBank. This enabled to confirm/infirm results of the morphologic identification and then, to establish the phylogenetic relationships between tsetse species. Morphologic features allowed to clearly distinguish all the tsetse species captured in the South Region of Cameroon, that is, <i>Glossina palpalis palpalis, G. pallicera</i>, <i>G. caliginea</i> and <i>G. nigrofusca</i>. In the northern area, <i>G. morsitans submorsitans</i> could also be distinguished from <i>G. palpalis palpalis</i>, <i>G. tachinoides</i> and <i>G. fuscipes</i>, but these three later could not be distinguished with routine morphological characters. The ITS1 length polymorphism was high among most of the studied species and allowed to identify the following similar species with a single PCR, that is, <i>G. palpalis palpalis</i> with 241 or 242 bp and <i>G. tachinoides</i> with 221 or 222 bp, <i>G. fuscipes</i> with 236 or 237 bp. We also updated the old distribution of tsetse species in the areas assessed, highlighting the presence of <i>G. palpalis palpalis</i> instead of <i>G. fuscipes</i> in Mbakaou, or in sympatry with <i>G. morsitans submorsitans</i> in Dodeo (northern Cameroon). This study confirms the presence of <i>G. palpalis palpalis</i> in the Adamawa Region of Cameroon. It highlights the limits of using morphological criteria to differentiate some tsetse species. Molecular tools based on the polymorphism of ITS1 of tsetse flies can differentiate tsetse species through a simple PCR before downstream analyses or vector control planning.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"216-226"},"PeriodicalIF":1.9,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First biochemical and behavioural analysis of the response of the scorpion Urophonius brachycentrus (Thorell: 1876) upon exposure to an organophosphate","authors":"Carlos F. Garcia,&nbsp;Andrés Ojanguren,&nbsp;Analía Seoane,&nbsp;Hernan Iuri,&nbsp;Rocio Gambaro,&nbsp;Gabriel Molina,&nbsp;Aldana Laino","doi":"10.1111/mve.12716","DOIUrl":"10.1111/mve.12716","url":null,"abstract":"<p>Scorpionism is an increasing public health problem in the world. Although no specific methodology or product is currently available for the control of those arachnids, the use of insecticides could be an effective tool. Chlorpyrifos is one of the insecticides used, but to date, whether scorpions recognise surfaces with that insecticide and how it affects their physiology and/or biochemistry is unknown. In the present study, we observed that scorpions recognise surfaces with 0.51 and 8.59 μg/cm² of chlorpyrifos and avoid those areas. The 0.51 μg/cm² concentration produced a decrease in acetylcholinesterase and an increase in catalase, superoxide dismutase and glutathione S-transferase, whereas the 8.59 μg/cm² concentration evoked a decrease in acetylcholinesterase and an increase in catalase and glutathione S-transferase. Using the comet assay, we observed that the insecticide at 0.17, 0.51 and 8.59 μg/cm² caused DNA damage. Finally, we found that the insecticide does not generate significant variations in glutathione peroxidase, glutathione reductase, the amount of protein or lipid peroxidation. The present results offer a comprehensive understanding of how scorpions respond, both at the biochemical and behavioural levels, when exposed to insecticides.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"291-302"},"PeriodicalIF":1.6,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140329941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular identification of Haemonchus contortus (Nematoda: Trichostrongylidae), a blood-sucking gastric nematode of artiodactyles, in the ground beetle Carabus granulatus (Coleoptera: Carabidae)","authors":"Joanna Werszko,&nbsp;Konrad Wilamowski,&nbsp;Olga Kraszewska,&nbsp;Sławomir Bakier,&nbsp;Anna Maria Pyziel","doi":"10.1111/mve.12715","DOIUrl":"10.1111/mve.12715","url":null,"abstract":"<p>Among gastrointestinal nematodes, <i>Haemonchus contortus</i> (Rudolphi) Cobb (order Strongylidae; family Trichostrongylidae) is one of pathogenic and economic importance in domestic and wild ruminants, including the European bison, <i>Bison bonasus</i> Linnaeus (order Cetartiodactyla; family Bovidae); a species on the International Union for Conservation of Nature's Red List of Threatened Species. <i>Carabus granulatus</i> Linnaeus (order Coleoptera; family Carabidae) is one of the most prevalent species of ground beetle, inhabiting a wide range of terrestrial ecosystems in Poland. Twenty-six ground beetles of this species inhabiting the Białowieża Primeval Forest in eastern Poland were screened for the presence of DNA of pathogenic gastrointestinal nematodes of ruminants. Extracted DNA was sequenced and compared to reference sequences. In six insects, the presence of <i>H. contortus</i> DNA was detected. The obtained nucleotide sequences were homologous to each other and to the majority of the published DNA sequences of <i>H. contortus</i> isolates. The sequences were also identical to a sequence of <i>H. contortus</i> isolated from European bison in Poland. The study provides the first molecular evidence of the presence of <i>H. contortus</i> DNA in <i>C. granulatus</i>. The finding suggests that ground beetles may play a role in the transmission dynamics of this parasite.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"361-365"},"PeriodicalIF":1.6,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12715","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140293936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possible role of haematophagous flies in the incidence of bovine teat papillomatosis","authors":"Mitsuhiro Iwasa,&nbsp;Yumeno Shido,&nbsp;Shinichi Hatama","doi":"10.1111/mve.12714","DOIUrl":"10.1111/mve.12714","url":null,"abstract":"<p>The relationship between the incidence of bovine teat papillomatosis and the activity of haematophagous flies was investigated in Japan. A total of 15,737 flies consisting of 33 species were collected by dry ice-baited mosquito net (DMN) trap and a sweep net from udders of cattle. <i>Simulium aokii</i> (Takahasi) of Simuliidae (black flies) was the predominant species, followed by <i>S. tobetsuense</i> Ono and <i>S. iwatense</i> (Shiraki). <i>Simulium aokii</i> had the highest peak in October, followed by September. Numbers of blood spots from the bites per teat in nulliparous cattle were significantly correlated with numbers of <i>S. aokii</i> collected by DMN trap. Numbers of teats with warts and spots of blood from the bites per teat were significantly more abundant in anterior teats than posterior teats. The average incidence of teat papillomatosis in nulliparous cattle was significantly higher than that in parous cattle, and the highest incidence by month was in May, followed by April. Although bovine papillomavirus (BPV) DNA was not detected in flies examined, the presence of black flies and blood spots from their bites were associated with subsequent high incidence of growing warts. In particular, it would pay to give attention to species such as <i>S. aokii</i> that severely attack udders in the present locality. Further investigations for the detection of BPV DNA from flies parasitizing on teats are needed.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"280-290"},"PeriodicalIF":1.6,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140158463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Mayaro virus vector competence of the mosquito Aedes aegypti (Linnaeus, 1762) populations in Argentine using dose–response assays","authors":"Mauricio Daniel Beranek,&nbsp;Octavio Giayetto,&nbsp;Sylvia Fischer,&nbsp;Adrián Diaz","doi":"10.1111/mve.12712","DOIUrl":"10.1111/mve.12712","url":null,"abstract":"<p>Mayaro virus (MAYV; <i>Alphavirus</i>: <i>Togaviridae</i>) is an emerging pathogen in Latin America, causing fever and polyarthritis. Sporadic outbreaks of MAYV have occurred in the region, with reported human cases being imported to Europe and North America. Although primarily a risk for those residing in the Amazon basin's tropical forests, recent reports highlight that urbanization would increase the risk of MAYV transmission in Latin America. Urban emergence depends on human susceptibility and the ability of mosquitos like <i>Aedes aegypti</i>  (Linnaeus, 1762) (Diptera: Culicidae) to transmit MAYV. Despite the absence of active MAYV transmission in Argentine, the risk of introduction is substantial due to human movement and the presence of <i>Ae. aegypti</i> in the region. This study aimed to evaluate the susceptibility of different Argentine <i>Ae. aegypti</i> populations to MAYV genotype L (MAYV-L) using dose–response assays and determine barriers to virus infection, dissemination and transmission. Immature mosquito stages were collected in Buenos Aires, Córdoba and Rosario cities. Female <i>Ae. aegypti</i> (F2) were orally infected by feeding on five concentrations of MAYV-L, ranging from 1.0 to 6.0 log₁₀ PFU/mL. Abdomens, legs and saliva were analysed using viral plaque assays. Results revealed that MAYV-L between infection and dissemination were associated with viral doses rather than the population origin. Infection rates varied between 3% and 65%, with a 50% infectious dose &gt;5.5 log₁₀ PFU/mL. Dissemination occurred at 39%, with a 50% dissemination dose of ~6.0 log₁₀ PFU/mL. Dissemination among infected mosquitoes ranged from 60% to 86%, and transmission from disseminated mosquitoes ranged from 11% to 20%. Argentine <i>Ae. aegypti</i> populations exhibited a need for higher viral doses of MAYV-L than those typically found in humans to become infected. In addition, only a small proportion of infected mosquitoes were capable of transmitting the virus. Understanding MAYV transmission in urban areas is crucial for public health interventions.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"234-243"},"PeriodicalIF":1.9,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140136964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of deltamethrin exposure on the cytochrome P450 monooxygenases of Aedes albopictus (Skuse) larvae from a dengue-endemic region of northern part of West Bengal, India","authors":"Prapti Das,&nbsp;Subhajit Das,&nbsp;Abhirup Saha,&nbsp;Debayan Raha,&nbsp;Dhiraj Saha","doi":"10.1111/mve.12713","DOIUrl":"10.1111/mve.12713","url":null,"abstract":"<p><i>Aedes albopictus</i> is highly prevalent in the northern part of West Bengal and is considered to be responsible for the recent dengue outbreaks in this region. Control of this vector is largely relied on the use of synthetic pyrethroids, which can lead to the development of resistance. In the present study, larvae of three wild <i>Ae. albopictus</i> populations from the dengue-endemic regions were screened for deltamethrin resistance, and the role of cytochrome P450 monooxygenases (CYPs) was investigated in deltamethrin exposed and unexposed larvae. Two populations were incipient resistant, and one population was completely resistant against deltamethrin. Monooxygenase titration assay revealed the involvement of CYPs in deltamethrin resistance along with an induction effect of deltamethrin exposure. Gene expression studies revealed differential expression of five CYP6 family genes, <i>CYP6A8</i>, <i>CYP6P12</i>, <i>CYP6A14</i>, <i>CYP6N3</i> and <i>CYP6N6</i>, with high constitutive expression of <i>CYP6A8</i> and <i>CYP6P12</i> in all the populations before and after deltamethrin exposure. From these findings, it was evident that CYPs play an important role in the development of deltamethrin resistance in the <i>Ae. albopictus</i> populations in this region.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 3","pages":"269-279"},"PeriodicalIF":1.6,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights from entire mitochondrial genome sequences into the phylogeny of ticks of the genera Haemaphysalis and Archaeocroton with the elevation of the subgenus Alloceraea Schulze, 1919 back to the status of a genus","authors":"Samuel Kelava,&nbsp;Dmitry A. Apanaskevich,&nbsp;Renfu Shao,&nbsp;Alexander W. Gofton,&nbsp;Ben J. Mans,&nbsp;Ernest J. M. Teo,&nbsp;Gerrut Norval,&nbsp;Dayana Barker,&nbsp;Ryo Nakao,&nbsp;Stephen C. Barker","doi":"10.1111/mve.12708","DOIUrl":"10.1111/mve.12708","url":null,"abstract":"<p>We used entire mitochondrial (mt) genome sequences (14.5–15 kbp) to resolve the phylogeny of the four main lineages of the Haematobothrion ticks: <i>Alloceraea, Archaeocroton, Bothriocroton</i> and <i>Haemaphysalis</i>. In our phylogenetic trees, <i>Alloceraea</i> was the sister to <i>Archaeocroton sphenodonti</i>, a tick of an archetypal reptile, the tuatara, from New Zealand, to the exclusion of the rest of the species of <i>Haemaphysalis</i>. The mt genomes of all four of the <i>Alloceraea</i> species that have been sequenced so far had a substantial insert, 132–312 bp, between the tRNA-Glu (E) gene and the <i>nad1</i> gene in their mt genomes. This insert was not found in any of the other eight subgenera of <i>Haemaphysalis</i>. The mt genomes of 13 species of <i>Haemaphysalis</i> from NCBI GenBank were added to the most recent data set on <i>Haemaphysalis</i> and its close relatives to help resolve the phylogeny of <i>Haemaphysalis</i>, including five new subgenera of <i>Haemaphysalis</i> not previously considered by other authors: <i>Allophysalis</i> (structurally primitive), <i>Aboimisalis</i> (structurally primitive), <i>Herpetobia</i> (structurally intermediate), <i>Ornithophysalis</i> (structurally advanced) and <i>Segalia</i> (structurally advanced). We elevated <i>Alloceraea</i> Schulze, 1919 to the status of genus because <i>Alloceraea</i> Schulze, 1919 is phylogenetically distinct from the other subgenera of <i>Haemaphysalis</i>. Moreover, we propose that the subgenus <i>Allophysalis</i> is the sister to the rest of the <i>Haemaphysalis</i> (14 subgenera) and that the ‘structurally primitive’ subgenera Hoogstraal and Kim comprise early diverging lineages. Our matrices of the pairwise genetic difference (percent) of mt genomes and partial 16S rRNA sequences indicated that the mt genome sequence of <i>Al</i>. <i>kitaokai</i> (gb# OM368280) may not be <i>Al</i>. <i>kitaokai</i> Hoogstraal, 1969 but rather another species of <i>Alloceraea</i>. In a similar way, the mt genome sequence of <i>H</i>. <i>(Herpetobia</i>) <i>nepalensis</i> Hoogstraal, 1962 (gb# NC_064124) was only 2% genetically different to that of <i>H</i>. (<i>Allophysalis</i>) <i>tibetensis</i> Hoogstraal, 1965 (gb# OM368293): this indicates to us that they are the same species. <i>Alloceraea cretacea</i> may be better placed in a genus other than <i>Alloceraea</i> Schulze, 1919. Reptiles may have been the host to the most recent common ancestor of <i>Archaeocroton</i> and <i>Alloceraea</i>.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"189-204"},"PeriodicalIF":1.9,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A perspective on the impacts of microplastics on mosquito biology and their vectorial capacity","authors":"Christopher M. Jones,&nbsp;Grant L. Hughes,&nbsp;Sylvester Coleman,&nbsp;Rosie Fellows,&nbsp;Richard S. Quilliam","doi":"10.1111/mve.12710","DOIUrl":"10.1111/mve.12710","url":null,"abstract":"<p>Microplastics (plastic particles &lt;5 mm) permeate aquatic and terrestrial ecosystems and constitute a hazard to animal life. Although much research has been conducted on the effects of microplastics on marine and benthic organisms, less consideration has been given to insects, especially those adapted to urban environments. Here, we provide a perspective on the potential consequences of exposure to microplastics within typical larval habitat on mosquito biology. Mosquitoes represent an ideal organism in which to explore the biological effects of microplastics on terrestrial insects, not least because of their importance as an infectious disease vector. Drawing on evidence from other organisms and knowledge of the mosquito life cycle, we summarise some of the more plausible impacts of microplastics including physiological, ecotoxicological and immunological responses. We conclude that although there remains little experimental evidence demonstrating any adverse effect on mosquito biology or pathogen transmission, significant knowledge gaps remain, and there is now a need to quantify the effects that microplastic pollution could have on such an important disease vector.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"138-147"},"PeriodicalIF":1.9,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/mve.12710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuticular extracts induce aggregation in head lice","authors":"Federico Gabriel Galassi,&nbsp;Maria Ines Picollo,&nbsp;Paola González-Audino","doi":"10.1111/mve.12711","DOIUrl":"10.1111/mve.12711","url":null,"abstract":"<p>The human head lice <i>Pediculus humanus capitis</i> (De Geer) (Phthiraptera: Pediculidae) are strict, obligate human ectoparasites that spends their entire life cycle in the host and cause skin irritation and derived infections. Despite the health-related importance, few studies have evaluated the chemical communication among these insects. Here, we evaluate the response of lice of both sexes to cuticular extracts using two solvents of different polarity (hexane and methanol). Cuticular extracts that elicited an attraction response towards head lice were analysed by gas chromatography–mass spectrometry (GC–MS) to determine the cuticular lipid profile. Both lice sexes were attracted to the hexane extracts but not the methanol extracts, suggesting the non-polarity of the compounds present in the cuticle. Chemical analyses of hexane extracts from males and females showed high similarity in major compounds. This study provides the first evidence that lice respond to cuticle extracts, which may be important to understand aggregation behaviour.</p>","PeriodicalId":18350,"journal":{"name":"Medical and Veterinary Entomology","volume":"38 2","pages":"227-233"},"PeriodicalIF":1.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}