Medical Molecular Morphology最新文献_第5页

Ultrastructure of the small vessels in the myocardium in a patient with fatal systemic capillary leak syndrome. 致死性全身毛细血管渗漏综合征患者心肌小血管超微结构的观察。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-04-29 DOI: 10.1007/s00795-025-00439-x

Hiroaki Kawano, Koichi Kawamura, Koji Maemura, Shinji Okano

引用次数: 0

Loss of miR-424 and miR-503 promotes decidualization of human endometrial stromal cells by increasing SCARA5 expression. miR-424和miR-503的缺失通过增加SCARA5的表达促进人子宫内膜基质细胞的蜕膜化。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-03-14 DOI: 10.1007/s00795-025-00431-5

Tetsu Yamaguchi, Masashi Takamura, Hideno Tochigi, Yumi Mizuno, Yosuke Mizuno, Tomomi Sato, Shunsuke Tamaru, Kazuya Kusama, Kazuhiro Tamura, Yoshimasa Kamei, Takeshi Kajihara

{"title":"Loss of miR-424 and miR-503 promotes decidualization of human endometrial stromal cells by increasing SCARA5 expression.","authors":"Tetsu Yamaguchi, Masashi Takamura, Hideno Tochigi, Yumi Mizuno, Yosuke Mizuno, Tomomi Sato, Shunsuke Tamaru, Kazuya Kusama, Kazuhiro Tamura, Yoshimasa Kamei, Takeshi Kajihara","doi":"10.1007/s00795-025-00431-5","DOIUrl":"10.1007/s00795-025-00431-5","url":null,"abstract":"This study aims to investigate the function of miR-424 and miR-503, identified as putative regulatory miRNAs of FOXO1, a key factor for decidualization. The expression of both miR-424 and miR-503 in human endometrial stromal cells (HESCs) were measured before and after decidualization. Then, HESCs were transfected with both miR-424 and miR-503 before decidualization. Quantitative reverse transcription PCR, actin staining analysis, migration assay, fluorescence immunostaining, and luciferase assay were performed. MiR-424 and miR-503 expression was decreased after decidualization. Overexpression of both miR-424 and miR-503 inhibited major decidual maker genes, including FOXO1, PRL, IGFBP1, WNT4, and SCARA5, and altered F-actin's subcellular distribution from the periphery to all over the cytoplasm, concomitantly increasing cell mobility. Moreover, immunohistochemical analysis revealed overexpression of both miRNAs resulted in FOXO1 protein accumulation in the cytoplasm. Knocking down FOXO1 decreased SCARA5 expression, revealing SCARA5 is a downstream target of FOXO1. In addition, a luciferase reporter assay confirmed that the 3'-untranslated region of FOXO1 mRNA is targeted by miR-424. These results suggest that both miRNAs may play an important role in endometrial decidualization by regulating transcriptional activity of FOXO1, which alters decidualization-related gene expression such as SCARA5.Abstract: Journal standard instruction requires an unstructured abstract; hence structured abstract changed to unstructured.Thank you for the correction. I approve this change.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"270-280"},"PeriodicalIF":1.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12644211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Keratin (OSCAR) immunohistochemistry as a reliable diagnostic marker for anaplastic thyroid carcinoma. 角蛋白（OSCAR）免疫组化作为间变性甲状腺癌的可靠诊断标志物。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-08-26 DOI: 10.1007/s00795-025-00447-x

Rin Yamada, Daiki Yoshii, Yoshihiro Komohara, Kaori Yukino, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita

引用次数: 0

Collision tumor consisting of primary mucoepidermoid carcinoma and adenocarcinoma in the lung: a case report and literature review. 肺原发性黏液表皮样癌与腺癌的碰撞瘤1例报告并文献复习。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-02-10 DOI: 10.1007/s00795-025-00422-6

Xiaomin Dai, Ruixia Xie, Linggong Zeng, Fang Peng

引用次数: 0

The role and potential mechanism of immunoglobulin G N-glycosylation in gastrointestinal tumors. 免疫球蛋白G - n -糖基化在胃肠道肿瘤中的作用及其潜在机制。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-08-25 DOI: 10.1007/s00795-025-00448-w

Yumeng Liu, Zejian Zhang, Xiequn Xu

{"title":"The role and potential mechanism of immunoglobulin G N-glycosylation in gastrointestinal tumors.","authors":"Yumeng Liu, Zejian Zhang, Xiequn Xu","doi":"10.1007/s00795-025-00448-w","DOIUrl":"10.1007/s00795-025-00448-w","url":null,"abstract":"Gastrointestinal tumors significantly contribute to cancer-related mortality worldwide. Early detection coupled with effective treatment significantly improves overall survival. Immunoglobulin G (IgG) N-glycosylation, a crucial post-translational modification, undergoes alterations in glycan structures. IgG N-glycosylation is associated with numerous physiological and pathological processes in the human body. Aberrant changes of IgG N-glycosylation play a key role in cancers given the involvement of glycans in cancer progression and immune modulation. These changes affect the binding of the Fc region of IgG to its receptor, in turn, affect the corresponding downstream effects, which are crucial in cancer immuno-surveillance and immune escape. This review aims to explore the latest advancements in understanding IgG N-glycosylation in gastrointestinal cancers, emphasizing its potential as a diagnostic biomarker and therapeutic target. The application of IgG N-glycosylation in clinical oncology could enhance early detection, improve therapeutic efficacy, and enable better monitoring of disease progression and recurrence. Furthermore, we summarized the research progression to provide novel insights into the potential regulatory mechanism of IgG N-glycosylation in gastrointestinal tumors. In all, IgG N-glycosylation holds significant promise for advancing cancer diagnosis and treatment. Further studies are required to fully elucidate its mechanisms and optimize its use in clinical practice.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"243-258"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of platelet-rich plasma, platelet-rich fibrin and concentrated growth factor on flap survival: a study in a rat dorsal cross-region perforator flap model. 富血小板血浆、富血小板纤维蛋白和浓缩生长因子对大鼠背侧跨区穿支皮瓣存活的影响

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-04-16 DOI: 10.1007/s00795-025-00436-0

Peiwen Li, Ruiqi Jin

{"title":"Effects of platelet-rich plasma, platelet-rich fibrin and concentrated growth factor on flap survival: a study in a rat dorsal cross-region perforator flap model.","authors":"Peiwen Li, Ruiqi Jin","doi":"10.1007/s00795-025-00436-0","DOIUrl":"10.1007/s00795-025-00436-0","url":null,"abstract":"Background: With the extensive application of flap surgery in clinical practice, it has been a matter of great concern to improve the survival rate of flap surgery for a long time. This study compared and explored the effect of the three generations of platelet concentrates (PCs), including platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF), on flap survival.Methods: After PRP, PRF, and CGF gels were observed by scanning electron microscope (SEM), their vascularizing effect were assessed by infrared thermal imager, flap survival experiment, arterial perfusion angiography, and immunohistochemical staining in a rat dorsal cross-region perforator flap model.Results: The fibrin of PRP gel showed irregular clumps and loose structure, while that of PRF and CGF gels formed 3D network structure with orderly arrangement and compact structure. In animal models, the use of all the three PCs can increase the number of vessels and the amount of blood perfusion in choke zones (all P < 0.05), thus improving the flap survival rate. Moreover, the effects of CGF and PRF were obviously better than those of PRP (all P < 0.05), but there was no significant difference between CGF and PRF (P > 0.05).Conclusions: PRP, PRF, and CGF all have a good effect on promoting vascularization, and can significantly improve the survival rate of dorsal cross-region perforator flap in rat model. Besides, PRF and CGF have greater potential in promoting vascularization than PRP.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"307-316"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Bax and Bcl-2 in the placenta from pre-term, term, and post-term deliveries: immunohistochemical analysis. Bax和Bcl-2在早产、足月和足月后胎盘中的表达：免疫组织化学分析

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1007/s00795-025-00429-z

Xvni Tang, Liyan Ye, Ting Yan, Xiujuan Zheng, Lichao Yuan

{"title":"Expression of Bax and Bcl-2 in the placenta from pre-term, term, and post-term deliveries: immunohistochemical analysis.","authors":"Xvni Tang, Liyan Ye, Ting Yan, Xiujuan Zheng, Lichao Yuan","doi":"10.1007/s00795-025-00429-z","DOIUrl":"10.1007/s00795-025-00429-z","url":null,"abstract":"The placenta is the most critical organ during pregnancy and its developmental status directly impacts fetal health. Placental dysfunction is associated with various pregnancy complications, including preterm birth, fetal growth restriction, and premature rupture of membranes (PROM). This study aimed to elucidate the expression changes of Bax and Bcl-2 and their association with pregnancy-related complications, providing new insights into placental dysfunction during pregnancy and offering a theoretical foundation for clinical diagnosis and prevention. The placental samples from 118 late pregnant women were retrospectively analyzed. They were assigned into pre-term, term, post-term, PROM, and non-PROM groups based on gestational age and the occurrence of PROM. Immunohistochemistry (IHC) staining was performed to gauge the Bax and Bcl-2 expressions in placenta. Receiver operating characteristic (ROC) curve was subsequently conducted to assess their associated with efficacy for PROM. The weight and volume of placentas in the pre-term group were sharply smaller to those in the term and post-term groups, with no apparent fibrosis or calcification observed. The term and post-term groups exhibited marked elevated Bax expression in the parturient group in contrast to the non-parturient group (P < 0.05), while there existed no substantial significance in Bcl-2 expression. The area under the curve (AUC) for Bax and Bcl-2 was 0.975 and 0.596, respectively, with a combined associated with value of 0.978, higher to single predictions. Bax and Bcl-2 expressions in late pregnancy placentas were associated with the onset of parturient status and PROM and their combined prediction exhibited accurate PROM predicted. These findings offered a new perspective for understanding the physiological and pathological changes of the placenta during pregnancy.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"259-269"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large number of CD68⁺/CD163⁺ Hofbauer cells and characteristic vascular structural alterations in the placental villi of cases with placenta accreta spectrum. 增生性胎盘患者胎盘绒毛中存在大量CD68+/CD163+霍夫鲍尔细胞及特征性血管结构改变。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-12-01 Epub Date: 2025-03-13 DOI: 10.1007/s00795-025-00432-4

Hazuki Kashiwagi, Kengo Shigehara, Terufumi Kubo, Yoshihiko Hirohashi, Tasuku Mariya, Kazuhiko Matsuo, Tomoyuki Minowa, Shin-Ichi Ishioka, Kenji Murata, Takayuki Kanaseki, Tomohide Tsukahara, Tadashi Hasegawa, Tsuyoshi Saito, Toshihiko Torigoe

{"title":"Large number of CD68+/CD163+ Hofbauer cells and characteristic vascular structural alterations in the placental villi of cases with placenta accreta spectrum.","authors":"Hazuki Kashiwagi, Kengo Shigehara, Terufumi Kubo, Yoshihiko Hirohashi, Tasuku Mariya, Kazuhiko Matsuo, Tomoyuki Minowa, Shin-Ichi Ishioka, Kenji Murata, Takayuki Kanaseki, Tomohide Tsukahara, Tadashi Hasegawa, Tsuyoshi Saito, Toshihiko Torigoe","doi":"10.1007/s00795-025-00432-4","DOIUrl":"10.1007/s00795-025-00432-4","url":null,"abstract":"Placenta accreta spectrum (PAS) is a serious disease leading to complications and maternal death. The objective of the study was to characterize the placental villi and blood vessels of PAS villi histopathologically. We investigated 10 cases of PAS (five cases of placenta increta, two cases of placenta accreta, and three cases of placenta percreta) histologically. Immunohistochemical staining using anti-CD68 or anti-CD163 antibodies was performed to detect and count Hofbauer cells. Immunohistochemical staining with an anti-CD34 antibody was used to detect vascular endothelial cells, and the number and area of vessels were analyzed. The numbers of CD68-positive or CD163-positive Hofbauer cells were larger in PAS cases compared with control cases. The vascular area in villi was smaller in PAS cases compared with control cases. The number of blood vessels in villi was slightly higher in PAS cases than in control cases. The numbers of Hofbauer cells and vessels in villi were larger in PAS cases compared with control cases, whereas the area of vessels in villi was smaller in PAS cases compared with control cases. Although their biological meaning is elusive, these findings provide novel insights into the pathogenesis of PAS, particularly regarding the role of Hofbauer cells in immune-suppressive role and angiogenesis and the alterations in vascular structure and hemodynamics in the chorionic villi.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"281-289"},"PeriodicalIF":1.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-dimensional architecture of the palmar plate of the thumb metacarpophalangeal joint in infant macaque. 幼猴拇指掌指关节掌板的三维结构。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-09-01 Epub Date: 2025-02-08 DOI: 10.1007/s00795-025-00423-5

Hiroko Sato, Tatsuo Shimada, Tsuguaki Hosoyama, Yutaro Shibuta, Nobuhiro Kaku

{"title":"Three-dimensional architecture of the palmar plate of the thumb metacarpophalangeal joint in infant macaque.","authors":"Hiroko Sato, Tatsuo Shimada, Tsuguaki Hosoyama, Yutaro Shibuta, Nobuhiro Kaku","doi":"10.1007/s00795-025-00423-5","DOIUrl":"10.1007/s00795-025-00423-5","url":null,"abstract":"The palmar plate is a crucial structural part of hand, associated with metacarpophalangeal and interphalangeal joints. Pediatric disorders involving the palmar plate of thumb metacarpophalangeal joint include trigger thumb, hyperextension, instability, and dislocation. While anatomical differences exist between children and adults, detailed microstructure evaluations in infants remain unexplored. In this study, we provide a histological and structural assessment of the previously unresolved microstructure of the palmar plate in the thumb metacarpophalangeal joint of infant Japanese macaques (Cercopithecidae, Macaca fuscata), a relevant model for human development. Histological staining (light microscopy) and scanning electron microscopy were employed to visualize the three-dimensional microstructure. The palmar plate of the infant macaque was found to contain (1) elastic fibers, (2) hyaline cartilage composed of type II collagen, and (3) type I collagen fibers arranged in distinct patterns. The cartilaginous region exhibited a reticulate fiber arrangement on its periphery, while the membranous region displayed dense and complex fibers on the proximal phalanx side and parallel on the metacarpal side, respectively. This is the first comprehensive three-dimensional investigation of the infant's thumb's palmar microanatomy, providing a foundation for understanding its development and implications for pediatric disorders.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"163-170"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D derivatives inhibit mesenchymal transition of mesothelial cells and mitigate peritoneal dissemination of ovarian cancer. 维生素D衍生物抑制间皮细胞间充质转化和减轻卵巢癌的腹膜播散。

IF 1.4 4区医学

Medical Molecular Morphology Pub Date : 2025-09-01 Epub Date: 2025-02-18 DOI: 10.1007/s00795-025-00424-4

Kazuhisa Fujita, Maia Hayashi, Masato Yoshihara, Satoshi Nomura, Kazuhisa Kitami, Emiri Miyamoto, Shohei Iyoshi, Kazumasa Mogi, Hiroki Fujimoto, Kaname Uno, Atsushi Kunishima, Yoshihiko Yamakita, Hiroyuki Tomita, Rino Tsutsumi, Ryota Sakamoto, Kazuo Nagasawa, Yusuke Masuo, Takumi Nishiuchi, Kiyosumi Shibata, Atsushi Enomoto, Hiroaki Kajiyama

{"title":"Vitamin D derivatives inhibit mesenchymal transition of mesothelial cells and mitigate peritoneal dissemination of ovarian cancer.","authors":"Kazuhisa Fujita, Maia Hayashi, Masato Yoshihara, Satoshi Nomura, Kazuhisa Kitami, Emiri Miyamoto, Shohei Iyoshi, Kazumasa Mogi, Hiroki Fujimoto, Kaname Uno, Atsushi Kunishima, Yoshihiko Yamakita, Hiroyuki Tomita, Rino Tsutsumi, Ryota Sakamoto, Kazuo Nagasawa, Yusuke Masuo, Takumi Nishiuchi, Kiyosumi Shibata, Atsushi Enomoto, Hiroaki Kajiyama","doi":"10.1007/s00795-025-00424-4","DOIUrl":"10.1007/s00795-025-00424-4","url":null,"abstract":"Ovarian cancer (OvCa) is a leading cause of gynecological cancer-related mortality, primarily due to peritoneal dissemination, which facilitates metastasis in the abdominal cavity. This study explored the potential of vitamin D and its synthetic derivatives in mitigating peritoneal dissemination by modulating the behavior of mesothelial cells (MCs). Vitamin D, through its receptor (VDR), is known to influence cancer progression, and our findings demonstrate that vitamin D derivatives can inhibit mesenchymal transition of MCs induced by TGF-β1, a key driver of peritoneal dissemination. This study used patient-derived primary MCs and in vivo mouse model to assess the effects of vitamin D derivatives on cell morphology, gene expression, and OvCa cell adhesion. Two vitamin D derivatives, VDR agonist, showed significant efficacy in maintaining epithelial-like MC morphology, reducing TGF-β1-induced changes, and inhibiting OvCa cell adhesion to the peritoneum, similar to calcitriol. Conversely, the VDR antagonist derivative induced MC apoptosis, highlighting the essential role of vitamin D in MC survival. These findings suggest that vitamin D derivatives could serve as promising therapeutic agents for OvCa by preserving peritoneal homeostasis and preventing metastasis. Further research is required to explore a broader range of derivatives and their underlying molecular mechanisms.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"171-182"},"PeriodicalIF":1.4,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0