Medical Molecular Morphology最新文献_第5页

Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development. 小鼠脑发育过程中神经发育障碍相关基因WAC的表达分析。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-04 DOI: 10.1007/s00795-023-00364-x

Masashi Nishikawa, Tohru Matsuki, Nanako Hamada, Atsuo Nakayama, Hidenori Ito, Koh-Ichi Nagata

{"title":"Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development.","authors":"Masashi Nishikawa, Tohru Matsuki, Nanako Hamada, Atsuo Nakayama, Hidenori Ito, Koh-Ichi Nagata","doi":"10.1007/s00795-023-00364-x","DOIUrl":"10.1007/s00795-023-00364-x","url":null,"abstract":"WAC is an adaptor protein involved in gene transcription, protein ubiquitination, and autophagy. Accumulating evidence indicates that WAC gene abnormalities are responsible for neurodevelopmental disorders. In this study, we prepared anti-WAC antibody, and performed biochemical and morphological characterization focusing on mouse brain development. Western blotting analyses revealed that WAC is expressed in a developmental stage-dependent manner. In immunohistochemical analyses, while WAC was visualized mainly in the perinuclear region of cortical neurons at embryonic day 14, nuclear expression was detected in some cells. WAC then came to be enriched in the nucleus of cortical neurons after birth. When hippocampal sections were stained, nuclear localization of WAC was observed in Cornu ammonis 1 - 3 and dentate gyrus. In cerebellum, WAC was detected in the nucleus of Purkinje cells and granule cells, and possibly interneurons in the molecular layer. In primary cultured hippocampal neurons, WAC was distributed mainly in the nucleus throughout the developing process while it was also localized at perinuclear region at 3 and 7 days in vitro. Notably, WAC was visualized in Tau-1-positive axons and MAP2-positive dendrites in a time-dependent manner. Taken together, results obtained here suggest that WAC plays a crucial role during brain development.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9803184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aquaporins contribute to vacuoles formation in Nile grass type II diabetic rats. 水通道蛋白促进尼罗草II型糖尿病大鼠液泡形成。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-26 DOI: 10.1007/s00795-023-00365-w

Kana Aihara, Yosuke Nakazawa, Shun Takeda, Natsuko Hatsusaka, Takanori Onouchi, Noriko Hiramatsu, Mayumi Nagata, Noriaki Nagai, Megumi Funakoshi-Tago, Naoki Yamamoto, Hiroshi Sasaki

{"title":"Aquaporins contribute to vacuoles formation in Nile grass type II diabetic rats.","authors":"Kana Aihara, Yosuke Nakazawa, Shun Takeda, Natsuko Hatsusaka, Takanori Onouchi, Noriko Hiramatsu, Mayumi Nagata, Noriaki Nagai, Megumi Funakoshi-Tago, Naoki Yamamoto, Hiroshi Sasaki","doi":"10.1007/s00795-023-00365-w","DOIUrl":"10.1007/s00795-023-00365-w","url":null,"abstract":"Regulation of ion and water microcirculation within the lens is tightly controlled through aquaporin channels and connexin junctions. However, cataracts can occur when the lens becomes cloudy. Various factors can induce cataracts, including diabetes which is a well-known cause. The most common phenotype of diabetic cataracts is a cortical and/or posterior subcapsular opacity. In addition to the three main types and two subtypes of cataracts, a vacuole formation is frequently observed; however, their origin remains unclear. In this study, we focused on the aquaporins and connexins involved in diabetes-induced cataracts and vacuoles in Nile grass type II diabetes. The results showed that the expression of aquaporin 0 and aquaporin 5 increased, and that of connexin 43 decreased in diabetic rat lenses. Additionally, aquaporin 0 and 5 were strongly localized in peripheral of vacuoles, suggesting that aquaporins are involved in vacuoles formation. Transillumination photography revealed large vacuoles at the tip of the Y-suture in the anterior capsule of the diabetic lens, and several small vacuoles were observed in the posterior capsule. Within the vacuoles, cytoplasmic degradation and aggregation of fibrous material were observed. Our findings suggest that aquaporins are potential candidate proteins for preventing vacuole formation.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10229190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromorphological observation of HLE cells under knockdown of Fascin using LV-SEM. fassin敲除后HLE细胞的微观形态学观察。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-08-01 DOI: 10.1007/s00795-023-00362-z

Yoshihiro Hayashi, Yumiko Yamamoto, Ichiro Murakami

{"title":"Micromorphological observation of HLE cells under knockdown of Fascin using LV-SEM.","authors":"Yoshihiro Hayashi, Yumiko Yamamoto, Ichiro Murakami","doi":"10.1007/s00795-023-00362-z","DOIUrl":"10.1007/s00795-023-00362-z","url":null,"abstract":"Liver cancer is one of the most prevalent cancers in Japan with hepatocellular carcinoma (HCC) as the major histological subtype. Successful novel treatments for HCC have been reported; however, recurrences or metastasis may occur, which results in poor prognoses and high mortality of HCC patients. Fascin, an actin-bundling protein, regulates cell adhesion, migration, and invasion. Its overexpression positively correlates with poor prognosis of malignant tumors, and Fascin is considered as one of the tumor biomarkers and therapeutic target proteins. In this study, we attempted to reveal the relationship between Fascin and HCC using HLE, one of the human HCC cell lines. We performed the study with classical immunocytochemistry and recently developed techniques, such as wound-healing assay, spheroid cultivation, and low-vacuum scanning electron microscopy (LV-SEM). Non-Fascin-knockdown (FKD) cell spheroid had a regular spherical appearance with tight cell-cell connections, while FKD cell spheroid had an irregular shape with loose cell-cell connections. Cells of non-FKD spheroid presented fibrous protrusions on the cell surface, contrarily, cells of FKD spheroids showed bulbous-shaped protrusions. Morphological observation of FKD and non-FKD HLE spheroids were performed using LV-SEM. Our study may help to reveal the roles of Fascin in the process of HCC formation and its malignancy.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to diagnosing crystal-storing histiocytosis: utility of scanning electron microscopy for formalin-fixed paraffin-embedded tissue specimens. 一种诊断晶体储存组织细胞增多症的新方法:扫描电子显微镜对福尔马林固定石蜡包埋组织标本的应用。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-03 DOI: 10.1007/s00795-023-00363-y

Kazufumi Magara, Akira Takasawa, Keisuke Kikuchi, Taro Sugawara, Taro Murakami, Daisuke Kyuno, Yusuke Ono, Kumi Takasawa, Yasunao Numata, Shigeru Sasaki, Hiroshi Nakase, Tadashi Hasegawa, Makoto Osanai

{"title":"A novel approach to diagnosing crystal-storing histiocytosis: utility of scanning electron microscopy for formalin-fixed paraffin-embedded tissue specimens.","authors":"Kazufumi Magara, Akira Takasawa, Keisuke Kikuchi, Taro Sugawara, Taro Murakami, Daisuke Kyuno, Yusuke Ono, Kumi Takasawa, Yasunao Numata, Shigeru Sasaki, Hiroshi Nakase, Tadashi Hasegawa, Makoto Osanai","doi":"10.1007/s00795-023-00363-y","DOIUrl":"10.1007/s00795-023-00363-y","url":null,"abstract":"Crystal-storing histiocytosis (CSH) is a rare disorder that shows infiltration of histiocytes with an aberrant cytoplasmic accumulation of crystalline structures and is often accompanied by lymphoproliferative-plasma cell disorders (LP-PCD) as background diseases. The diagnosis of CSH requires identification of crystalline structures that accumulate in the infiltrating histiocytes, which may be challenging by optical microscopy alone. In this case report, we describe an atypical course of systemic CSH with multifocal fibrosclerosis of an unknown background disease that was diagnosed by ultrastructural observation, including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), in pathological autopsy. In addition, crystalline structures were successfully identified by scanning electron microscopic observations using formalin-fixed and paraffin-embedded (FFPE) tissue from biopsy specimens taken before death. Since CSH was identified by SEM in a tiny biopsy specimen, observation of histiocytic infiltrative lesions by SEM using FFPE tissue may lead to early detection of and initiation of treatment for CSH.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9738149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast-track preparation of lung specimens for electron microscope observations of the pulmonary endothelial glycocalyx. 快速制备肺标本，用于电镜观察肺内皮糖萼。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-05 DOI: 10.1007/s00795-023-00360-1

Mone Wakatsuki, Takashi Takaki, Akira Ushiyama, Kazuho Honda, Takehiko Iijima

{"title":"Fast-track preparation of lung specimens for electron microscope observations of the pulmonary endothelial glycocalyx.","authors":"Mone Wakatsuki, Takashi Takaki, Akira Ushiyama, Kazuho Honda, Takehiko Iijima","doi":"10.1007/s00795-023-00360-1","DOIUrl":"10.1007/s00795-023-00360-1","url":null,"abstract":"The glycocalyx (GCX) covers the luminal surface of blood vessels and regulates vascular permeability. As GCX degradation predicts various types of vasculopathy, confirming the presence of this structure is useful for diagnosis. Since the GCX layer is very fragile, careful fixation is necessary to preserve its structure. We explored appropriate and feasible methodologies for visualizing the GCX layer using lung tissue specimens excised from anesthetized mice. Each specimen was degassed and immersed in Alcian blue (ALB) fixative solution, and then observed using electron microscopy. Specimens from septic mice were prepared as negative GCX controls. Using these immersion-fixed specimens, the GCX layer was successfully observed using both transmission and scanning electron microscopy; these observations were similar to those obtained using the conventional method of lanthanum perfusion fixation. Spherical aggregates of GCX were observed in the septic mouse specimens, and the GCX density was lower in the septic specimens than in the non-septic specimens. Of note, the presently reported methodology reduced the specimen preparation time from 6 to 2 days. We, therefore, concluded that our novel method could be applied to human lung specimens and could potentially contribute to the further elucidation of vasculopathies.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9808015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: A case of coexistent poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), usual-type adenocarcinoma, and squamous cell carcinoma in situ of the cervix. 更正:1例宫颈低分化腺鳞癌(玻璃状细胞癌)、普通型腺癌和鳞状细胞原位癌共存。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 DOI: 10.1007/s00795-023-00358-9

Kouki Habara, Asami Nishikori, Jin Kiyama, Manami Nakashima, Masanori Koda, Kenji Sasaki, Tomohisa Sakashita, Norifumi Tanaka, Shuji Yonehara

引用次数: 0

A case of coexistent poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), usual-type adenocarcinoma, and squamous cell carcinoma in situ of the cervix. 低分化腺鳞癌(玻璃状细胞癌)、普通型腺癌和宫颈原位鳞状细胞癌共存1例。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 Epub Date: 2023-05-02 DOI: 10.1007/s00795-023-00354-z

Kouki Habara, Asami Nishikori, Jin Kiyama, Manami Nakashima, Masanori Koda, Kenji Sasaki, Tomohisa Sakashita, Norifumi Tanaka, Shuji Yonehara

{"title":"A case of coexistent poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), usual-type adenocarcinoma, and squamous cell carcinoma in situ of the cervix.","authors":"Kouki Habara, Asami Nishikori, Jin Kiyama, Manami Nakashima, Masanori Koda, Kenji Sasaki, Tomohisa Sakashita, Norifumi Tanaka, Shuji Yonehara","doi":"10.1007/s00795-023-00354-z","DOIUrl":"10.1007/s00795-023-00354-z","url":null,"abstract":"Poorly differentiated adenosquamous carcinoma (glassy cell carcinoma) of the cervix is extremely rare, accounting for 1-2% of all cervical cancers. Herein, we report a case with coexistent poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), \"usual-type\" adenocarcinoma, and squamous cell carcinoma in situ of the cervix. A female patient in her 60 s was referred to our hospital and diagnosed with poorly differentiated adenosquamous carcinoma based on cervical cytology and biopsy. The tumor was classified as clinical stage IB1 cervical cancer following magnetic resonance imaging; radical hysterectomy was performed. Histopathological examination revealed poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), usual-type adenocarcinoma, and squamous cell carcinoma in situ, all coexisting. All carcinoma regions showed identical sizes to high-risk human papillomavirus (HPV) in fragment analysis. The patient is currently alive, without evidence of recurrence, 31 months post surgery. In this case, three different carcinomas coexisted. Fragment analysis of the patient's HPV status suggested that all carcinomas were related to an infection with the same high-risk HPV type. To determine the precise mechanism of tumor development, i.e., whether the tumors were of the mixed or collision type, further studies are needed, including clonal analysis for the loss of heterozygosity pattern.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10415468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9997338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraductal papilloma with atypical ductal hyperplasia and neuroendocrine differentiation as a possible precursor lesion of solid papillary carcinoma. 导管内乳头状瘤伴不典型导管增生和神经内分泌分化，可能是实体乳头状癌的前兆病变。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 Epub Date: 2023-06-07 DOI: 10.1007/s00795-023-00357-w

Yutaro Mihara, Rin Yamaguchi, Ryuji Takahashi, Yuta Yano, Hirohisa Yano

引用次数: 0

FGF4 and FGF9 have synergistic effects on odontoblast differentiation. FGF4和FGF9对成牙本质细胞分化具有协同作用。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 Epub Date: 2023-04-03 DOI: 10.1007/s00795-023-00351-2

Tatsuki Hoshino, Shoko Onodera, Motoyoshi Kimura, Makoto Suematsu, Tatsuya Ichinohe, Toshifumi Azuma

{"title":"FGF4 and FGF9 have synergistic effects on odontoblast differentiation.","authors":"Tatsuki Hoshino, Shoko Onodera, Motoyoshi Kimura, Makoto Suematsu, Tatsuya Ichinohe, Toshifumi Azuma","doi":"10.1007/s00795-023-00351-2","DOIUrl":"10.1007/s00795-023-00351-2","url":null,"abstract":"The purpose of this study was to investigate whether fibroblast growth factor 4 (FGF4) and FGF9 are active in dentin differentiation. Dentin matrix protein 1 (Dmp1) -2A-Cre transgenic mice, which express the Cre-recombinase in Dmp1-expressing cells, were crossed with CAG-tdTomato mice as reporter mouse. The cell proliferation and tdTomato expressions were observed. The mesenchymal cell separated from neonatal molar tooth germ were cultured with or without FGF4, FGF9, and with or without their inhibitors ferulic acid and infigratinib (BGJ398) for 21 days. Their phenotypes were evaluated by cell count, flow cytometry, and real-time PCR. Immunohistochemistry for FGFR1, 2, and 3 expression and the expression of DMP1 were performed. FGF4 treatment of mesenchymal cells obtained promoted the expression of all odontoblast markers. FGF9 failed to enhance dentin sialophosphoprotein (Dspp) expression levels. Runt-related transcription factor 2 (Runx2) was upregulated until day 14 but was downregulated on day 21. Compared to Dmp1-negative cells, Dmp1-positive cells expressed higher levels of all odontoblast markers, except for Runx2. Simultaneous treatment with FGF4 and FGF9 had a synergistic effect on odontoblast differentiation, suggesting that they may play a role in odontoblast maturation.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9968978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

S100A8 and S100A9 are associated with endometrial shedding during menstruation. S100A8和S100A9与月经期间子宫内膜脱落有关。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 Epub Date: 2023-04-22 DOI: 10.1007/s00795-023-00355-y

Kazumori Arai, Aki Kubota, Tomohiro Iwasaki, Akihiro Sonoda, Junichi Sakane

{"title":"S100A8 and S100A9 are associated with endometrial shedding during menstruation.","authors":"Kazumori Arai, Aki Kubota, Tomohiro Iwasaki, Akihiro Sonoda, Junichi Sakane","doi":"10.1007/s00795-023-00355-y","DOIUrl":"10.1007/s00795-023-00355-y","url":null,"abstract":"Matrix metalloproteinases (MMPs) and their major source, endometrial stromal cells (ESCs), play important roles in menstruation. However, other mechanisms in endometrial shedding may be unexplored. This study focused on four proteins: S100A8 and S100A9 (alarmins) are binding partners and induce MMPs, MMP-3 cycle-dependently plays a key role in the proteolytic cascade, and CD147, which has S100A9 as its ligand, induces MMPs. Immunostaining for these proteins was performed on 118 resected specimens. The percentage and location of each positive reaction in ESCs were measured and compared using Image J. The influence of leukocytes on S100A8 or S100A9 immunopositivity was also examined. From the premenstrual phase, S100A8 and MMP-3 began to have overlapping expressions in ESCs of the superficial layer, and ESC detachment was found within these sites. S100A9 was expressed from the late secretory phase and CD147 already from earlier. Later, the expression sites of S100A9 and CD147 included those of S100A8. Before menstruation, S100A8 or S100A9 expression was not affected by leukocytes. These results suggest that the local formation of S100A8/S100A9 complex, which occurs specifically in ESCs upon progesterone withdrawal, induces the local expression of MMP-3 and serves as a switch to the lysis phase.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9974965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0