Medical Molecular Morphology最新文献_第5页

High ambient temperature may induce presbyopia via TRPV1 activation. 高环境温度可能会通过激活 TRPV1 引发老花眼。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-07-09 DOI: 10.1007/s00795-024-00391-2

Yosuke Nakazawa, Yumika Kuno, Hibiki Shimada, Noriaki Nagai, Noriko Hiramatsu, Shun Takeda, Naoki Yamamoto, Megumi Funakoshi-Tago, Hiroshi Sasaki

{"title":"High ambient temperature may induce presbyopia via TRPV1 activation.","authors":"Yosuke Nakazawa, Yumika Kuno, Hibiki Shimada, Noriaki Nagai, Noriko Hiramatsu, Shun Takeda, Naoki Yamamoto, Megumi Funakoshi-Tago, Hiroshi Sasaki","doi":"10.1007/s00795-024-00391-2","DOIUrl":"10.1007/s00795-024-00391-2","url":null,"abstract":"The prevalence of presbyopia and nuclear cataracts (NUC) is reported to be higher in tropical areas than that in other regions, suggesting a potential influence of high temperatures on lens health. Transient receptor potential vanilloid (TRPV) channels play a crucial role in detecting ambient temperatures across various species, with TRPV1 and TRPV4 expressed in lens epithelial cells. In this study, we investigated whether ambient temperatures affect TRPV1 and TRPV4 activity in the lens, potentially contributing to the development of presbyopia and NUC. We conducted experiments using cultured human lens epithelial cell lines under different temperature conditions. Our results revealed that the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) and p38 pathways, downstream molecules of TRPV1, were activated, while Src family kinase, a downstream molecule of TRPV4, was inhibited at 37.5 °C culture compared to 35.0 °C. Confocal microscope images demonstrated higher expression of TRPV1 in 3D-structured cells under high-temperature culture conditions. Additionally, in organ culture lenses, higher elasticity was observed at elevated temperatures compared to that at lower temperatures. These results suggest that high ambient temperatures may induce lens sclerosis via TRPV1 activation, potentially contributing to the development of presbyopia and NUC.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"268-276"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive analysis of transcription factors involved in odontoblast differentiation mechanism. 全面分析参与骨母细胞分化机制的转录因子

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-07-11 DOI: 10.1007/s00795-024-00389-w

Haruka Nakazato, Shoko Onodera, Natsuko Aida, Masahiro Furusawa, Toshifumi Azuma

{"title":"Comprehensive analysis of transcription factors involved in odontoblast differentiation mechanism.","authors":"Haruka Nakazato, Shoko Onodera, Natsuko Aida, Masahiro Furusawa, Toshifumi Azuma","doi":"10.1007/s00795-024-00389-w","DOIUrl":"10.1007/s00795-024-00389-w","url":null,"abstract":"Primary cultured odontoblasts rapidly lose their tissue-specific phenotype. To identify transcription factors (TF) that are important for the maintenance of the odontoblast phenotype, primary cultures of C57BL/6 J mouse dental mesenchymal cells (DMC) were isolated, and expression of TF and odontoblast marker genes in cells immediately after isolation and 2 days after culture were comprehensively evaluated and compared using RNA-sequencing (RNA-seq). The expression of odontoblast markers in mouse dental mesenchymal cells decreased rapidly after isolation. In addition, the expression of Hedgehog-related, Notch-related, and immediate- early gene (IEG)-related transcription factors significantly decreased. Forced expression of these genes in lentiviral vectors, together with fibroblast growth factor 4 (FGF4), fibroblast growth factor 9 (FGF9), and the Wnt pathway activator CHIR99021, significantly induced the expression of odontogenic marker genes. These results indicate, for the first time, that Notch signaling and early genes may be important for maintaining odontoblast cultures. Furthermore, simultaneous stimulation of FGF, Wnt, Hedgehog, Notch pathways, and IEG transcription factors cooperatively promoted the maintenance of the odontoblast phenotype. These results suggest that the Hedgehog and Notch signaling pathways may play an important role in maintaining odontoblast phenotypes, in addition to FGF and Wnt signaling.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"253-267"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of histopathological classification and establishment of prognostic indicators of gastric adenocarcinoma based on deep learning algorithm. 基于深度学习算法的胃腺癌组织病理学分类鉴定和预后指标建立。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-08-01 DOI: 10.1007/s00795-024-00399-8

Zhihui Wang, Hui Peng, Jie Wan, Anping Song

{"title":"Identification of histopathological classification and establishment of prognostic indicators of gastric adenocarcinoma based on deep learning algorithm.","authors":"Zhihui Wang, Hui Peng, Jie Wan, Anping Song","doi":"10.1007/s00795-024-00399-8","DOIUrl":"10.1007/s00795-024-00399-8","url":null,"abstract":"The aim of this study is to establish a deep learning (DL) model to predict the pathological type of gastric adenocarcinoma cancer based on whole-slide images(WSIs). We downloaded 356 histopathological images of gastric adenocarcinoma (STAD) patients from The Cancer Genome Atlas database and randomly divided them into the training set, validation set and test set (8:1:1). Additionally, 80 H&E-stained WSIs of STAD were collected for external validation. The CLAM tool was used to cut the WSIs and further construct the model by DL algorithm, achieving an accuracy of over 90% in identifying and predicting histopathological subtypes. External validation results demonstrated the model had a certain generalization ability. Moreover, DL features were extracted from the model to further investigate the differences in immune infiltration and patient prognosis between the two subtypes. The DL model can accurately predict the pathological classification of STAD patients, and provide certain reference value for clinical diagnosis. The nomogram combining DL-signature, gene-signature and clinical features can be used as a prognostic classifier for clinical decision-making and treatment.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"286-298"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and ultrastructural morphological analyses of highly metamorphosed Aspergillus fumigatus on human formalin-fixed paraffin-embedded tissue. 人福尔马林固定石蜡包埋组织上高度变态曲霉的分子和超微结构形态学分析

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-08-14 DOI: 10.1007/s00795-024-00402-2

Kazuhiro Matsumoto, Masanori Goto, Yuki Kamikokura, Kumi Takasawa, Nobuyuki Kobayashi, Tomoyuki Aoyama, Taro Murakami, Masayo Kamikokura, Yuta Ikechi, Tomoki Kawahata, Kitaru Tanaka, Sayaka Takatori, Daisuke Fujishiro, Kensaku Okamoto, Yuichi Makino, Yuji Nishikawa, Akira Takasawa

{"title":"Molecular and ultrastructural morphological analyses of highly metamorphosed Aspergillus fumigatus on human formalin-fixed paraffin-embedded tissue.","authors":"Kazuhiro Matsumoto, Masanori Goto, Yuki Kamikokura, Kumi Takasawa, Nobuyuki Kobayashi, Tomoyuki Aoyama, Taro Murakami, Masayo Kamikokura, Yuta Ikechi, Tomoki Kawahata, Kitaru Tanaka, Sayaka Takatori, Daisuke Fujishiro, Kensaku Okamoto, Yuichi Makino, Yuji Nishikawa, Akira Takasawa","doi":"10.1007/s00795-024-00402-2","DOIUrl":"10.1007/s00795-024-00402-2","url":null,"abstract":"Invasive fungal infections including invasive pulmonary aspergillosis (IPA) generally have a poor prognosis, because the fungi spread throughout various organs. Therefore, it is important to accurately identify the fungal species for treatment. In this article, we present the results of pathological and molecular morphological analyses that were performed to elucidate the cause of respiratory failure in a patient who died despite suspicion of IPA and treatment with micafungin (MCFG). Pathological analysis revealed the existence of cystic and linear fungi in lung tissue. The fungi were identified as Aspergillus fumigatus (A. fumigatus) by partial sequencing of genomic DNA. Correlative light microscopy and electron microscopy (CLEM) analysis confirmed that fungi observed with light microscopy can also be observed with scanning electron microscopy (SEM) using formalin-fixed paraffin-embedded tissue sections. SEM revealed an atypical ultrastructure of the fungi including inhomogeneous widths, rough surfaces, and numerous cyst-like structures of various sizes. The fungi showed several morphological changes of cultured A. fumigatus treated with MCFG that were previously reported. Our results indicate that integrated analysis of ultrastructural observation by SEM and DNA sequencing may be an effective tool for analyzing fungi that are difficult to identify by conventional pathological analysis.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"326-332"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and pathological analyses of 14 cases of angiomatoid fibrous histiocytoma. 14 例血管瘤样纤维组织细胞瘤的临床和病理分析。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-07-30 DOI: 10.1007/s00795-024-00400-4

Qiang Zeng, Jie-Zhen Li, Guo-Ping Li, Yu-Peng Chen, Fang-Ling Song, Feng Gao

{"title":"Clinical and pathological analyses of 14 cases of angiomatoid fibrous histiocytoma.","authors":"Qiang Zeng, Jie-Zhen Li, Guo-Ping Li, Yu-Peng Chen, Fang-Ling Song, Feng Gao","doi":"10.1007/s00795-024-00400-4","DOIUrl":"10.1007/s00795-024-00400-4","url":null,"abstract":"Angiomatoid fibrous histiocytoma (AFH) is a soft tissue tumor of uncertain differentiation. Although its prognosis is good, its diagnosis and differential diagnosis remain a challenge, particularly for tumors with an atypical morphology. We evaluated the clinicopathological characteristics of 14 AFH cases and examined the key factors in its diagnosis or differential diagnosis. The cohort comprised 6 men and 8 women aged 9-65 years (average age: 31.2 years). Most of the tumors (11/14, 79%) were located in soft tissues, whereas 3/14 (21%) were located in the lung (1 case) and brain (2 cases). Tumor cells were spindle-shaped to epithelioid, with a visible fibrous capsule (9/14, 64%), hemorrhagic gap (9/14, 64%), lymphocyte sleeve (7/14, 50%), necrosis (3/14, 21%), and infiltrative boundary (4/14, 29%). The tumors expressed desmin (10/14, 71%) and exhibited low levels of Ki-67. 13 cases (93%) displayed ESWSR1 gene rearrangement. At follow-up, 1 case (7%) experienced local tumor recurrence. AFH is a rare intermediate tumor. Its pathological diagnosis requires a comprehensive analysis of histological, immunophenotypic, and molecular genetic features to avoid misdiagnosis. Our study has further enriched the histological features of AFH, emphasizing the importance of differential diagnosis and providing a reference for clinical practice.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"299-305"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of miR-34a, RASSF1A and E-cadherin in relation to PRB in endometrioid carcinoma and its precursor. 子宫内膜样癌及其前体中 miR-34a、RASSF1A 和 E-cadherin 的表达与 PRB 的关系。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-07-23 DOI: 10.1007/s00795-024-00397-w

Mona Mostafa Ahmed, Amr A Awd, Muhannad Mohamed Elsayed, Basma A Ibrahim, Hanim M Abdelnour

{"title":"Expression of miR-34a, RASSF1A and E-cadherin in relation to PRB in endometrioid carcinoma and its precursor.","authors":"Mona Mostafa Ahmed, Amr A Awd, Muhannad Mohamed Elsayed, Basma A Ibrahim, Hanim M Abdelnour","doi":"10.1007/s00795-024-00397-w","DOIUrl":"10.1007/s00795-024-00397-w","url":null,"abstract":"The current study aims to evaluate the levels of miR-34a, RASSF1A, and E-cadherin in relation to the levels of isoform B of progesterone receptor (PRB) in endometrioid carcinoma (EC) and atypical hyperplasia (AEH) and their association with clinicopathological parameters. 105 cases (35 EC, 35 AEH, and 35 control) were involved in this study. Cases of AEH received treatment, and other samples were obtained after 6 months to assess the response. E-cadherin and PRB were assessed by immunohistochemistry (IHC), RASSFA methylation by MSP-PCR, and its serum level by ELISA and miR-34a via quantitative PCR. The expressions of miR-34a, RASSF1A, E-cadherin, and PRB differ among the studied groups; all were higher in normal compared with AEH and EC, with a statistically significant difference. The higher PRB expression and decreased miR-34a and RASSF1A expression were associated with resistance to hormonal therapy in AEH. High PRB in EC is associated with lower RASSFA1, E-cadherin, and miR-34a. Decreased expressions of RASSF1A, miR-34a, and E-cadherin had a significant connection to advanced stages. Expression of PRB and miR-34a and serum levels of RASSF1A predict response to treatment in cases of AEH. High PRB and low E-cadherin expression are associated with progressive disease in EC.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"277-285"},"PeriodicalIF":1.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NF1 mutation and TUBB3 amplification in gastric histiocytic sarcoma: a case report and literature review. 胃组织细胞肉瘤中的NF1突变和TUBB3扩增：病例报告和文献综述。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-09-01 Epub Date: 2024-06-24 DOI: 10.1007/s00795-024-00393-0

Yi Yang, Wei Fan, Xiaoping Liu, Qiongrong Chen

{"title":"NF1 mutation and TUBB3 amplification in gastric histiocytic sarcoma: a case report and literature review.","authors":"Yi Yang, Wei Fan, Xiaoping Liu, Qiongrong Chen","doi":"10.1007/s00795-024-00393-0","DOIUrl":"10.1007/s00795-024-00393-0","url":null,"abstract":"Histiocytic sarcoma is a rare neoplasm of mature histiocytes with an aggressive clinical course and poor response to treatment. Primary gastric histiocytic sarcoma is rarer and just reported sporadically.Histiocytic sarcoma is a rare neoplasm of mature histiocytes with an aggressive clinical course and poor response to treatment. Primary gastric histiocytic sarcoma is rarer and just reported sporadically. A case of a 71-year-old female admitted with a one-year history of upper abdominal pain exacerbated after meals. After CT scans revealed a bulged mass at the lesser curvature of the gastric body, the patient underwent endoscopic submucosal dissection. Microscopically, non-cohesive neoplastic cells diffusely infiltrated lamina propria and submucosa, and diffusely expressed LCA, CD4, CD163, CD68 (KP1), Cyclin D1, Lysozyme, and Vimentin. PD-L1 (22CS) expression evaluated as CPS 60. The final pathological diagnosis was gastric histiocytic sarcoma. Subsequently, next-generation sequencing identified a nonsense mutation in exon 21 of NF1 gene [c.2446C > T (p.R816*)] and the TUBB3 gene amplification (copy number: 4.55). The patient refused further treatment and died of the tumor half a year later. This case broadens the spectrum of differential diagnosis of gastric cancer and emphasizes the value of immunohistochemical and molecular tests in the accurate diagnosis of histiocytic sarcoma. Furthermore, we performed literature review of 11 cases of gastric histiocytic sarcoma so as to strengthen the understanding of the clinicopathologic features, treatment, and prognosis.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"244-251"},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141446487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liver fibrosis analysis using digital pathology. 利用数字病理学进行肝纤维化分析。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-09-01 Epub Date: 2024-07-09 DOI: 10.1007/s00795-024-00395-y

Hisamitsu Miyaaki, Satoshi Miuma, Masanori Fukusima, Ryu Sasaki, Masafumi Haraguchi, Yasuhiko Nakao, Yuko Akazawa, Kazuhiko Nakao

引用次数: 0

Primary hepatobiliary mucoepidermoid carcinoma: a case report and review of literature. 原发性肝胆粘液表皮样癌：病例报告和文献综述。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-09-01 Epub Date: 2024-06-21 DOI: 10.1007/s00795-024-00390-3

Zihan Li, Hiep Nguyen Canh, Khuyen Nguyen Thi, Kenta Takahashi, Quynh Nguyen Thi, Dong Le Thanh, Rui Yang, Yasunori Sato, Kenichi Harada

{"title":"Primary hepatobiliary mucoepidermoid carcinoma: a case report and review of literature.","authors":"Zihan Li, Hiep Nguyen Canh, Khuyen Nguyen Thi, Kenta Takahashi, Quynh Nguyen Thi, Dong Le Thanh, Rui Yang, Yasunori Sato, Kenichi Harada","doi":"10.1007/s00795-024-00390-3","DOIUrl":"10.1007/s00795-024-00390-3","url":null,"abstract":"Hepatobiliary mucoepidermoid carcinoma is a rare malignant tumor comprising mucous, intermediate, and epidermoid cells. Herein, we presented a case of primary liver mucoepidermoid carcinoma preoperatively misdiagnosed as conventional intrahepatic cholangiocarcinoma. A 67-year-old male was admitted to our hospital. Preoperative laboratory tests showed increased aspartate transaminase, alanine transaminase, and carbohydrate antigen 19-9. Abdominal Computer Tomography revealed a 4.8 × 4.9 cm liver mass in segment VI. A preliminary diagnosis of intrahepatic cholangiocarcinoma was made, with undergoing partial hepatectomy. However, on histopathology, the tumor comprised a mixture of epidermoid, mucous, and intermediate cells with diffuse infiltrating at the tumor margin. On special stains, mucous and intermedia cells were positive for mucicarmine and Alcian blue, whereas epidermoid cells were positive for Keratin 5/6 and p63. Intermediate cells are also positive for p63. All tumor cells were positive for Keratin 7. The Ki-67 index was 35%. The final diagnosis was primary hepatic mucoepidermoid carcinoma. Although rare, hepatic mucoepidermoid carcinoma should be considered in the intrahepatic cholangiocarcinoma differential diagnosis. We reviewed previous studies and found that hepatobiliary mucoepidermoid carcinoma is more likely to originate from the biliary tract adjacent to the tumor.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"233-243"},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141432185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P4HA2 involved in SLUG-associated EMT predicts poor prognosis of patients with KRAS-positive colorectal cancer. 参与 SLUG 相关 EMT 的 P4HA2 预测了 KRAS 阳性结直肠癌患者的不良预后。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2024-09-01 Epub Date: 2024-03-24 DOI: 10.1007/s00795-024-00385-0

Heba El-Deek Mohammed El-Deek, Maha Salah El-Naggar, Aiat Morsy Mohamed Morsy, Mayada Fawzy Sedik, Heba Ahmed Osman, Asmaa M Ahmed

{"title":"P4HA2 involved in SLUG-associated EMT predicts poor prognosis of patients with KRAS-positive colorectal cancer.","authors":"Heba El-Deek Mohammed El-Deek, Maha Salah El-Naggar, Aiat Morsy Mohamed Morsy, Mayada Fawzy Sedik, Heba Ahmed Osman, Asmaa M Ahmed","doi":"10.1007/s00795-024-00385-0","DOIUrl":"10.1007/s00795-024-00385-0","url":null,"abstract":"This study aimed to examine the immunohistochemical expression of epithelial-mesenchymal transition biomarkers: P4HA2 and SLUG in colorectal carcinoma (CRC) specimens, then to assess their relation to clinicopathological features including KRAS mutations and patients' survival, and finally to study the correlation between them in CRC. The result of this study showed that SLUG and P4HA2 were significantly higher in association with adverse prognostic factors: presence of lympho-vascular invasion, perineural invasion, higher tumor budding, tumor stage, presence of lymph node metastasis, and presence of distant metastasis. CRC specimens with KRAS mutation were associated with significant higher SLUG and P4HA2 expression. High expression of both SLUG and P4HA2 was significantly unfavorable prognostic indicator as regards overall survival (OS) and disease-free survival (DFS). In KRAS mutated cases, high P4HA2 expression was the only significant poor prognostic indicator as regarding DFS. In conclusions, our data highlight that both SLUG and P4HA2 expression may serve as potentially important poor prognostic biomarkers in CRC and targeting these molecules may be providing a novel therapeutic strategy. In KRAS mutation group, high P4HA2 expression is the only independent prognostic factor for tumor recurrence, so it can be suggested to be a novel target for therapy.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"167-176"},"PeriodicalIF":1.2,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0