Expression of miR-34a, RASSF1A and E-cadherin in relation to PRB in endometrioid carcinoma and its precursor.

IF 1.2 4区医学 Q3 PATHOLOGY

Medical Molecular Morphology Pub Date : 2024-12-01 Epub Date: 2024-07-23 DOI:10.1007/s00795-024-00397-w

Mona Mostafa Ahmed, Amr A Awd, Muhannad Mohamed Elsayed, Basma A Ibrahim, Hanim M Abdelnour

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Abstract

The current study aims to evaluate the levels of miR-34a, RASSF1A, and E-cadherin in relation to the levels of isoform B of progesterone receptor (PRB) in endometrioid carcinoma (EC) and atypical hyperplasia (AEH) and their association with clinicopathological parameters. 105 cases (35 EC, 35 AEH, and 35 control) were involved in this study. Cases of AEH received treatment, and other samples were obtained after 6 months to assess the response. E-cadherin and PRB were assessed by immunohistochemistry (IHC), RASSFA methylation by MSP-PCR, and its serum level by ELISA and miR-34a via quantitative PCR. The expressions of miR-34a, RASSF1A, E-cadherin, and PRB differ among the studied groups; all were higher in normal compared with AEH and EC, with a statistically significant difference. The higher PRB expression and decreased miR-34a and RASSF1A expression were associated with resistance to hormonal therapy in AEH. High PRB in EC is associated with lower RASSFA1, E-cadherin, and miR-34a. Decreased expressions of RASSF1A, miR-34a, and E-cadherin had a significant connection to advanced stages. Expression of PRB and miR-34a and serum levels of RASSF1A predict response to treatment in cases of AEH. High PRB and low E-cadherin expression are associated with progressive disease in EC.

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子宫内膜样癌及其前体中 miR-34a、RASSF1A 和 E-cadherin 的表达与 PRB 的关系。

本研究旨在评估子宫内膜样癌（EC）和非典型增生（AEH）中miR-34a、RASSF1A和E-cadherin的水平与孕酮受体异构体B（PRB）水平的关系及其与临床病理参数的关联。本研究共涉及 105 个病例（35 个 EC、35 个 AEH 和 35 个对照组）。AEH病例接受治疗，6个月后采集其他样本以评估反应。E-cadherin和PRB通过免疫组织化学（IHC）进行评估，RASSFA甲基化通过MSP-PCR进行评估，其血清水平通过ELISA进行评估，miR-34a通过定量PCR进行评估。miR-34a、RASSF1A、E-cadherin和PRB的表达在研究组间存在差异；与AEH和EC相比，正常组的表达均较高，差异有统计学意义。PRB表达较高、miR-34a和RASSF1A表达较低与AEH对激素治疗的耐受性有关。EC中的高PRB与较低的RASSFA1、E-cadherin和miR-34a有关。RASSF1A、miR-34a和E-cadherin的表达降低与晚期有显著关系。PRB和miR-34a的表达以及血清中RASSF1A的水平可预测AEH病例的治疗反应。PRB的高表达和E-cadherin的低表达与EC的进展性疾病有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Molecular Morphology 医学-病理学

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.