Medical Molecular Morphology最新文献

Effect of S1P/S1PR on bone metabolism in bisphosphonate-related osteonecrosis of the jaws. S1P/S1PR对双磷酸盐相关性颌骨骨坏死骨代谢的影响。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-04-24 DOI: 10.1007/s00795-026-00461-7

Ting Guo, Yuhao Wang, Dianri Wang, Wenbin Yang, Xiye Sun, Jiyuan Liu, Jian Pan

{"title":"Effect of S1P/S1PR on bone metabolism in bisphosphonate-related osteonecrosis of the jaws.","authors":"Ting Guo, Yuhao Wang, Dianri Wang, Wenbin Yang, Xiye Sun, Jiyuan Liu, Jian Pan","doi":"10.1007/s00795-026-00461-7","DOIUrl":"https://doi.org/10.1007/s00795-026-00461-7","url":null,"abstract":"Bisphosphonate-related osteonecrosis of the jaws (BRONJ) is a major adverse effect of bisphosphonates, yet its underlying pathogenesis remains poorly understood. Bone metabolism and remodeling relies on the interaction between osteoblasts (OBs) and osteoclasts (OCs). Sphingosine 1-phosphate (S1P), a bioactive sphingolipid metabolite, is an important mediator of OC-OB communication. In this study, we aimed to investigate the role of the S1P/S1P receptor (S1PR) axis in the development of BRONJ. A co-culture system was used to examine the interaction between OCs and OBs. Western blot and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of related proteins and messenger ribonucleic acids (mRNAs). Finally, an in vivo BRONJ mouse model was used to validate the role of S1P/S1PR axis in disease progression. In our study, we showed that zoledronate (ZOL) promoted S1P secretion from OCs and enhanced the migration of osteoclast precursor cells (OCPs) through S1PR signaling. In addition, OCs promoted the excessive osteogenic differentiation and migration of OBs via S1P/S1PR axis. Importantly, pharmacological inhibition of S1PR facilitated the recovery of BRONJ-like lesions in vivo. In conclusion, these findings indicate that the S1P/S1PR axis plays an important role in the pathogenesis of BRONJ and may represent a potential therapeutic target for its treatment.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aggressive multiple myeloma with lymph node involvement, loss of CD138, and adipophilin-positive cytoplasmic vacuolization: a case report. 侵袭性多发性骨髓瘤伴淋巴结受累、CD138缺失和嗜脂素阳性细胞质空泡化1例报告。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-04-22 DOI: 10.1007/s00795-026-00460-8

Yoshihiko Kondo, Seiichiro Nakabeppu, Hiromu Yano, Kenji Ishitsuka, Tadahito Urakado, Yukio Fujiwara, Masahiro Yamamoto, Kennosuke Karube, Yoshihiro Komohara

{"title":"Aggressive multiple myeloma with lymph node involvement, loss of CD138, and adipophilin-positive cytoplasmic vacuolization: a case report.","authors":"Yoshihiko Kondo, Seiichiro Nakabeppu, Hiromu Yano, Kenji Ishitsuka, Tadahito Urakado, Yukio Fujiwara, Masahiro Yamamoto, Kennosuke Karube, Yoshihiro Komohara","doi":"10.1007/s00795-026-00460-8","DOIUrl":"https://doi.org/10.1007/s00795-026-00460-8","url":null,"abstract":"We describe a rare and aggressive case of multiple myeloma (MM) characterized by extensive lymph node involvement, loss of CD138 expression, and adipophilin (ADP)-positive cytoplasmic vacuolization, highlighting the role of lipid metabolism in disease aggressiveness. An 83-year-old woman presented with painless cervical lymphadenopathy and widespread osteolytic lesions. Bone marrow examination confirmed MM, while lymph node biopsy showed diffuse infiltration of atypical lymphoid cells with numerous tingible body macrophages, initially mimicking a high-grade lymphoma. Immunophenotyping showed CD3/CD5/CD20/CD23 negativity, focal CD138/CD79a positivity, diffuse MUM1 and κ-light chain positivity, and a high Ki-67 index. Compared with bone marrow plasma cells, lymph node MM cells exhibited prominent cytoplasmic vacuoles and nuclear enlargement. Immunohistochemistry demonstrated ADP positivity in lymph node lesions but not in bone marrow MM cells, suggesting metabolic reprogramming toward lipid utilization. Despite anti-myeloma therapy, the disease rapidly progressed, and the patient died within two months. This case underscores the clinical significance of CD138 down-regulation as a marker of dedifferentiation and poor prognosis, and suggests that altered lipid metabolism may contribute to the aggressiveness of metastatic MM. To the best of our knowledge, this is the first MM case with lymph node involvement showing CD138 down-regulation and ADP positivity.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Fbxw7 disrupts lipid homeostasis and autophagy in hepatocellular carcinoma cells. Fbxw7的缺失会破坏肝细胞癌细胞的脂质稳态和自噬。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-26 DOI: 10.1007/s00795-026-00457-3

Yoshihiro Hayashi, Yumiko Yamamoto, Ichiro Murakami

引用次数: 0

Novel autoantibodies in autoimmune hepatitis: from diagnostic challenges to molecular and spatial pathology. 自身免疫性肝炎中的新型自身抗体：从诊断挑战到分子和空间病理。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-26 DOI: 10.1007/s00795-026-00459-1

Kazumichi Abe, Jun Wada, Manabu Hayashi, Tatsuro Sugaya, Naoto Abe, Masashi Fujita, Hiromasa Ohira

引用次数: 0

Upregulated Klotho expression reflects a potential compensatory renal response in Trimethyltin-exposed rats. Klotho表达上调反映了三甲基锡暴露大鼠潜在的代偿性肾反应。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-26 DOI: 10.1007/s00795-026-00458-2

Devi Purnamasari Sasongko, Dian Eurike Septyaningtrias, Rina Susilowati

引用次数: 0

Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs. 马拉塞人上皮细胞克隆细胞条件培养基对牙胚牙釉质结晶的影响。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-01 Epub Date: 2025-07-07 DOI: 10.1007/s00795-025-00444-0

Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh

{"title":"Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs.","authors":"Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh","doi":"10.1007/s00795-025-00444-0","DOIUrl":"10.1007/s00795-025-00444-0","url":null,"abstract":"We have previously isolated the epithelial rests of Malassez (ERM) clone cells with strong Amelx expression, named as ERM-2, from the crude ERM cells. In the present study, we examined whether conditioned medium (CM) derived from cultured ERM-2 promotes the crystallization of immature enamel in tooth germs. Tooth germs from postnatal day 3 mice were incubated with ERM-2 conditional medium (CM). ERM-2 cells were transfected with si-RNA targeting specific enamel matrix proteins (EMPs). After 2 days of incubation, each CM was collected and employed to culture the tooth germs. The surface layers of the enamel structure were examined with a scanning electron microscope (SEM). Tooth germs cultured with ERM-2 CM on days 3 and 7 showed elongation and densification of the columnar structures in SEM analysis. The columnar structures became denser and aggregated forming a HAP-like hexagonal columnar structure 14 days after culture in ERM-2 CM. In contrast, no clear columnar structures were observed in ERM-2 CM with si-RNA of each EMPs. In conclusion, the present study demonstrated that CM derived from ERM-2 could form enamel-like structures on the surface of the tooth germ. ERM-2 may provide the possibility for the clinical use of enamel regeneration.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"42-56"},"PeriodicalIF":1.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies. 代谢功能障碍相关脂肪变性肝病活检中肝纤维化模式对肝癌发生的预测

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-01 Epub Date: 2025-06-05 DOI: 10.1007/s00795-025-00440-4

Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao

{"title":"Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies.","authors":"Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao","doi":"10.1007/s00795-025-00440-4","DOIUrl":"10.1007/s00795-025-00440-4","url":null,"abstract":"This study aimed to investigate carcinogenesis-related fibrosis patterns in liver biopsy tissues from patients with metabolic dysfunction-associated steatotic liver disease (MASLD) by comprehensively measuring and quantifying various fibrosis patterns using artificial intelligence. Liver biopsy tissues from 13 patients with advanced fibrosis at MASLD diagnosis were subjected to collagen quantification and morphological and structural fiber characteristic evaluation using FibroNest (PharmaNest, Princeton, NJ, USA), which was described using up to seven quantitative fibrosis parameters (qFPs). The collagen-fibrosis composite score (FCS), morphometric-FCS, architecture-FCS, and phenotypic-FCS (Ph-FCS) were compared between patients with and without hepatocellular carcinoma (HCC). The collagen quantification alone could not discriminate between HCC and non-HCC cases. Regarding the individual qFPs of morphological fiber characteristics, the kurtosis and skewness of fiber twists were significantly lower in HCC cases than in non-HCC cases. In HCC cases, fiber width and density kurtosis tended to be larger, whereas fiber length kurtosis tended to be smaller than those in non-HCC cases. Ph-FCS could discriminate HCC from non-HCC at a threshold of 4.2, with 85% sensitivity and 100% specificity. A combination of fiber morphology and structural characteristics predicted HCC development with higher accuracy and might help define carcinogenic risk groups among patients with MASLD.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"26-33"},"PeriodicalIF":1.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minichromosome maintenance 4 is associated with poor survival and stemness of patients with pancreatic cancer. 小染色体维持与胰腺癌患者的低生存率和干细胞性相关。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-01 Epub Date: 2025-04-28 DOI: 10.1007/s00795-025-00438-y

Yuto Fujiki, Akira Ishikawa, Narutaka Katsuya, Yuki Shiwa, Takafumi Fukui, Kazuya Kuraoka, Takeshi Sudo, Sho Tazuma, Yasutaka Ishii, Shiro Oka, Wataru Yasui, Shinji Mii

{"title":"Minichromosome maintenance 4 is associated with poor survival and stemness of patients with pancreatic cancer.","authors":"Yuto Fujiki, Akira Ishikawa, Narutaka Katsuya, Yuki Shiwa, Takafumi Fukui, Kazuya Kuraoka, Takeshi Sudo, Sho Tazuma, Yasutaka Ishii, Shiro Oka, Wataru Yasui, Shinji Mii","doi":"10.1007/s00795-025-00438-y","DOIUrl":"10.1007/s00795-025-00438-y","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is one of the most well-known cancer types, with a persistently poor 5-year survival rate. We previously reported MCM4 as a molecule associated with cancer stem cells; however, its role in PDAC has not been reported. Therefore, in this study, we aimed to fill this gap in the literature. We analyzed MCM4 expression in 81 PDAC samples using immunohistochemistry (IHC). The functional role of MCM4 in PDAC was investigated using RNA interference in PDAC cell lines. Additionally, a single-cell analysis was conducted by downloading data from six PDAC cases. On IHC, high MCM4 expression was observed in 42 out of 81 (51.9%) PDAC cases. MCM4-positive PDAC was significantly associated with a higher pN grade. Furthermore, high MCM4 expression was linked to a significantly poorer prognosis and was identified as an independent prognostic factor in multivariate analysis. In PDAC cell lines, MCM4 knockdown impairs cell growth and spheroid formation. Single-cell analysis also revealed that MCM4-expressing cells were located upstream of the trajectory, with a cluster showing a correlation with KIFC1, which has been reported to be associated with cancer stemness. These results indicated the significance of MCM4 expression in PDAC and its association with cancer stemness.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"8-19"},"PeriodicalIF":1.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors. 免疫组织化学和分子方法评价PRDM10基因重排在无法分类的未分化软组织肿瘤中的作用。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-01 Epub Date: 2025-06-25 DOI: 10.1007/s00795-025-00442-2

Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen

{"title":"Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors.","authors":"Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen","doi":"10.1007/s00795-025-00442-2","DOIUrl":"10.1007/s00795-025-00442-2","url":null,"abstract":"Soft tissue sarcomas are heterogenous groups of tumors that show variable morphology as well as clinical behavior. Morphological features do not always directly reflect clinical behavior. Certain mesenchymal tumors exhibit an indolent clinical course. Among them are superficial CD34-positive fibroblastic tumors characterized by PRDM10 fusion. In our study, we aimed to detect PRDM10 gene rearrangement in superficial CD34-positive fibroblastic tumors and other pleomorphic sarcomas included in its differential diagnosis by immunohistochemistry and Fluorescence in situ hybridization. Totally, 33 cases were enrolled into this study. The results showed that two cases diagnosed as superficial CD34-positive fibroblastic tumor and two cases diagnosed as undifferentiated pleomorphic sarcoma have PRDM10 gene rearrangement. Immunohistochemically, not all rearranged tumors showed PRDM10 staining that suggests a low sensitivity of PRDM10 antibody. In conclusion, we suggested that PRDM10 gene rearrangement is not limited to superficial CD34-positive fibroblastic tumors; undifferentiated pleomorphic sarcomas may exhibit this molecular alteration and immunohistochemistry has lower sensitivity than fluorescence in situ hybridization.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"20-25"},"PeriodicalIF":1.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaplastic thyroid carcinoma with osteoclast-like giant cells: a case report and a study of a potential therapeutic approach. 甲状腺间变性癌伴破骨细胞样巨细胞：一例报告及一种潜在治疗方法的研究。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2026-03-01 Epub Date: 2025-07-24 DOI: 10.1007/s00795-025-00443-1

Kaori Yukino, Yoshihiro Komohara, Shukang Zhao, Rin Yamada, Yukio Fujiwara, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita

{"title":"Anaplastic thyroid carcinoma with osteoclast-like giant cells: a case report and a study of a potential therapeutic approach.","authors":"Kaori Yukino, Yoshihiro Komohara, Shukang Zhao, Rin Yamada, Yukio Fujiwara, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita","doi":"10.1007/s00795-025-00443-1","DOIUrl":"10.1007/s00795-025-00443-1","url":null,"abstract":"Anaplastic thyroid carcinoma (ATC) is a highly aggressive neoplasm with no effective treatment options. ATC with osteoclast-like giant cells (OGCs; ATC/OGC) is a rare variant of ATC, and no detailed pathological examination has been reported to date. A 59-year-old woman presented with sudden neck swelling. Computed tomography revealed a 5 cm tumor in the thyroid gland, which was surgically resected. Pathological examination revealed a diagnosis of ATC/OGC. The patient succumbed to progressive lung metastases within four months despite postoperative lenvatinib therapy. Immunohistochemical (IHC) examination indicated absence of PD-L1 expression in the OGCs, which comprised the majority of the tumor, with only sparse T cell infiltration in the area occupied by OGCs. Increased TGF-β expression was observed in the area containing OGCs, and both OGCs and infiltrating myeloid cells, including CD1a/CD11c-positive dendritic cells and CD68/CD163/CD204-positive macrophages, appeared to produce TGF-β. Pathological analysis of this case suggests that OGCs might be involved in immune suppression by secreting TGF-β, potentially serving as a critical cytokine in the immunosuppressive microenvironment of ATC/OGC. TGF-β-targeted therapy might be useful in the treatment of this very rare subtype of ATC.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"64-70"},"PeriodicalIF":1.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0