Medical Molecular Morphology最新文献

Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs. 马拉塞人上皮细胞克隆细胞条件培养基对牙胚牙釉质结晶的影响。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-07-07 DOI: 10.1007/s00795-025-00444-0

Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh

{"title":"Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs.","authors":"Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh","doi":"10.1007/s00795-025-00444-0","DOIUrl":"https://doi.org/10.1007/s00795-025-00444-0","url":null,"abstract":"We have previously isolated the epithelial rests of Malassez (ERM) clone cells with strong Amelx expression, named as ERM-2, from the crude ERM cells. In the present study, we examined whether conditioned medium (CM) derived from cultured ERM-2 promotes the crystallization of immature enamel in tooth germs. Tooth germs from postnatal day 3 mice were incubated with ERM-2 conditional medium (CM). ERM-2 cells were transfected with si-RNA targeting specific enamel matrix proteins (EMPs). After 2 days of incubation, each CM was collected and employed to culture the tooth germs. The surface layers of the enamel structure were examined with a scanning electron microscope (SEM). Tooth germs cultured with ERM-2 CM on days 3 and 7 showed elongation and densification of the columnar structures in SEM analysis. The columnar structures became denser and aggregated forming a HAP-like hexagonal columnar structure 14 days after culture in ERM-2 CM. In contrast, no clear columnar structures were observed in ERM-2 CM with si-RNA of each EMPs. In conclusion, the present study demonstrated that CM derived from ERM-2 could form enamel-like structures on the surface of the tooth germ. ERM-2 may provide the possibility for the clinical use of enamel regeneration.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat stroke-induced hepatic lipid dysregulation: histological and lipidomic insights. 中暑引起的肝脂质失调：组织学和脂质组学的见解。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-07-02 DOI: 10.1007/s00795-025-00441-3

Takahiro Deguchi, Hiroki Tanaka, Kie Horioka, Chihiro Matsuhisa, Akira Hayakawa, Shuhei Takauji, Shimpei Watanabe, Masanori Goto, Yumiko Fujii, Kumi Takasawa, Akira Takasawa

{"title":"Heat stroke-induced hepatic lipid dysregulation: histological and lipidomic insights.","authors":"Takahiro Deguchi, Hiroki Tanaka, Kie Horioka, Chihiro Matsuhisa, Akira Hayakawa, Shuhei Takauji, Shimpei Watanabe, Masanori Goto, Yumiko Fujii, Kumi Takasawa, Akira Takasawa","doi":"10.1007/s00795-025-00441-3","DOIUrl":"https://doi.org/10.1007/s00795-025-00441-3","url":null,"abstract":"Global warming has increased summer temperatures, leading to a rise in heat stroke-related deaths in Japan. Heat stroke disrupts the body's adaptation to high temperatures, often resulting in severe complications, including liver damage and even death. However, despite the increasing incidence, pathological autopsies remain rare, and the histological changes associated with heat stroke are poorly understood. In this study, we investigated the pathogenesis of heat stroke using a mouse model. Mice were exposed to 45 °C for 30 min and dissected immediately or 24, 48, and 72 h post-exposure. Histological analysis revealed significant lipid accumulation in hepatocytes surrounding the central vein at 24, 48, and 72 h. At 24 h, hepatocytes also exhibited features of early degeneration, including cytoplasmic lysis and chromatin condensation. Lipidomics analysis of liver tissue collected 24 h post-exposure demonstrated a marked increase in 27-hydroxycholesterol levels. These results indicate that heat stress rapidly disrupts hepatic lipid homeostasis, causing cellular damage and metabolic remodeling. The observed lipid accumulation, including elevated 27-hydroxycholesterol, may play dual roles in mediating inflammation and serving as a protective response. Our findings provide new insight into the pathogenesis of heat stroke-induced liver injury and suggest potential molecular targets for early diagnosis and intervention.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors. 免疫组织化学和分子方法评价PRDM10基因重排在无法分类的未分化软组织肿瘤中的作用。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-25 DOI: 10.1007/s00795-025-00442-2

Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen

{"title":"Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors.","authors":"Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen","doi":"10.1007/s00795-025-00442-2","DOIUrl":"https://doi.org/10.1007/s00795-025-00442-2","url":null,"abstract":"Soft tissue sarcomas are heterogenous groups of tumors that show variable morphology as well as clinical behavior. Morphological features do not always directly reflect clinical behavior. Certain mesenchymal tumors exhibit an indolent clinical course. Among them are superficial CD34-positive fibroblastic tumors characterized by PRDM10 fusion. In our study, we aimed to detect PRDM10 gene rearrangement in superficial CD34-positive fibroblastic tumors and other pleomorphic sarcomas included in its differential diagnosis by immunohistochemistry and Fluorescence in situ hybridization. Totally, 33 cases were enrolled into this study. The results showed that two cases diagnosed as superficial CD34-positive fibroblastic tumor and two cases diagnosed as undifferentiated pleomorphic sarcoma have PRDM10 gene rearrangement. Immunohistochemically, not all rearranged tumors showed PRDM10 staining that suggests a low sensitivity of PRDM10 antibody. In conclusion, we suggested that PRDM10 gene rearrangement is not limited to superficial CD34-positive fibroblastic tumors; undifferentiated pleomorphic sarcomas may exhibit this molecular alteration and immunohistochemistry has lower sensitivity than fluorescence in situ hybridization.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies. 代谢功能障碍相关脂肪变性肝病活检中肝纤维化模式对肝癌发生的预测

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-05 DOI: 10.1007/s00795-025-00440-4

Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao

{"title":"Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies.","authors":"Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao","doi":"10.1007/s00795-025-00440-4","DOIUrl":"https://doi.org/10.1007/s00795-025-00440-4","url":null,"abstract":"This study aimed to investigate carcinogenesis-related fibrosis patterns in liver biopsy tissues from patients with metabolic dysfunction-associated steatotic liver disease (MASLD) by comprehensively measuring and quantifying various fibrosis patterns using artificial intelligence. Liver biopsy tissues from 13 patients with advanced fibrosis at MASLD diagnosis were subjected to collagen quantification and morphological and structural fiber characteristic evaluation using FibroNest (PharmaNest, Princeton, NJ, USA), which was described using up to seven quantitative fibrosis parameters (qFPs). The collagen-fibrosis composite score (FCS), morphometric-FCS, architecture-FCS, and phenotypic-FCS (Ph-FCS) were compared between patients with and without hepatocellular carcinoma (HCC). The collagen quantification alone could not discriminate between HCC and non-HCC cases. Regarding the individual qFPs of morphological fiber characteristics, the kurtosis and skewness of fiber twists were significantly lower in HCC cases than in non-HCC cases. In HCC cases, fiber width and density kurtosis tended to be larger, whereas fiber length kurtosis tended to be smaller than those in non-HCC cases. Ph-FCS could discriminate HCC from non-HCC at a threshold of 4.2, with 85% sensitivity and 100% specificity. A combination of fiber morphology and structural characteristics predicted HCC development with higher accuracy and might help define carcinogenic risk groups among patients with MASLD.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relation in MreB and intrabacterial nanotransportation system for VacA in Helicobacter pylori. 幽门螺旋杆菌中的 MreB 与 VacA 的细菌内纳米运输系统的关系。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2024-12-20 DOI: 10.1007/s00795-024-00416-w

Hong Wu, Yoshihiko Fujioka, Noritaka Iwai, Shoichi Sakaguchi, Youichi Suzuki, Takashi Nakano

引用次数: 0

Clinicopathological significance of sulfite oxidase expression in surgically resected lung adenocarcinoma. 亚硫酸盐氧化酶在肺腺癌手术切除组织中表达的临床病理意义。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2024-12-09 DOI: 10.1007/s00795-024-00413-z

Saeko Tokisawa, Reiichiro Kondo, Masamichi Nakayama, Sachiko Ogasawara, Kenta Murotani, Masahiro Mitsuoka, Tomoaki Hoshino, Hirohisa Yano, Jun Akiba

{"title":"Clinicopathological significance of sulfite oxidase expression in surgically resected lung adenocarcinoma.","authors":"Saeko Tokisawa, Reiichiro Kondo, Masamichi Nakayama, Sachiko Ogasawara, Kenta Murotani, Masahiro Mitsuoka, Tomoaki Hoshino, Hirohisa Yano, Jun Akiba","doi":"10.1007/s00795-024-00413-z","DOIUrl":"10.1007/s00795-024-00413-z","url":null,"abstract":"Aim: The coenzyme sulfite oxidase (SUOX), located in mitochondria, plays a role in redox and metabolism. Its expression has been associated with cancer progression and prognosis. Lung cancer has a high incidence rate and poor prognosis. We aim to clarify its expression in lung adenocarcinomas and investigated the utility of SUOX expression as a recurrence factor in operable lung adenocarcinoma.Methods: We used 60 formalin-fixed paraffin-embedded samples of operable primary lung adenocarcinoma between 2017 and 2018 to immunohistochemically assess SUOX expression levels. Patients were classified into a high or low SUOX expression group, and the associations of SUOX expression with clinicopathological findings and recurrence were analyzed.Results: We revealed that high SUOX expression was significantly (p < 0.05) associated with sex, low Brinkman index, histological type, histological grade and positive for epidermal growth factor receptor (EGFR) mutation. High SUOX expression (HR = 10.218, 95% CI 1.758‒59.376, p = 0.0096) and pathological Stage (HR = 7.538, 95% CI 1.95‒29.14, p = 0.0034) were independently associated with relapse free survival.Conclusion: High SUOX expression may be a new indicator of recurrence risk in surgically resected lung adenocarcinomas.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"106-113"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Androgens modulate endometrial function. 雄激素调节子宫内膜功能。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2025-03-10 DOI: 10.1007/s00795-025-00430-6

Ko Yamagata, Yousuke Mizuno, Yumi Mizuno, Shunnsuke Tamaru, Takeshi Kajihara

引用次数: 0

Coexistence of complete intestinal tract, prostatic tissue, prostatic urethra and bladder structure in ovarian mature cystic teratoma: a case report. 卵巢成熟囊性畸胎瘤中同时存在完整的肠道、前列腺组织、前列腺尿道和膀胱结构：一份病例报告。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2024-11-11 DOI: 10.1007/s00795-024-00409-9

Kaho Ito, Kohei Nakamura, Takeshi Kajihara, Yu Shinozaki, Johji Imura, Hiroshi Sakuma

引用次数: 0

In vivo corneal confocal microscopy findings in cases with pterygium: a case-control study. 体内角膜共聚焦显微镜在翼状胬肉病例中的发现：一项病例对照研究。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2024-12-04 DOI: 10.1007/s00795-024-00412-0

Halit Öcal, Erbil Seven, Serek Tekin, Muhammed Batur

{"title":"In vivo corneal confocal microscopy findings in cases with pterygium: a case-control study.","authors":"Halit Öcal, Erbil Seven, Serek Tekin, Muhammed Batur","doi":"10.1007/s00795-024-00412-0","DOIUrl":"10.1007/s00795-024-00412-0","url":null,"abstract":"This study aimed to evaluate corneal findings of pterygium cases using in vivo confocal microscopy (IVCM) which is a non-invasive and repeatable method. In this case-control study, 54 patients diagnosed with pterygium and 50 healthy controls were investigated, between 2020 and 2021. After a comprehensive ophthalmological examination, the central corneas of all participants were evaluated by corneal IVCM. Surface epithelial cell, wing cells, basal epithelial cells, nerve fiber density, nerve branch density, anterior and posterior stroma keratocyte densities were measured. Corneas from cases with and without pterygium were similar in terms of age (p = 0.306) and gender (p = 0.564). No statistically significant differences were found in the epithelium cell counts including surface, wing, and basal cells between the two groups (p = 0.950, p = 0.334, and p = 0.624, respectively). No statistically significant differences were observed in subepithelial nerve fiber density (p = 0.849), nerve branch density (p = 0.752), and posterior stromal keratocyte density (p = 0.683) between corneas from cases with and without pterygium. However, corneas from cases with pterygium had significantly higher scores in anterior stromal keratocyte density than those from cases without pterygium (p = 0.045). Corneas from grades 1, 2, and 3 pterygium were similar in cell counts, anterior and posterior stromal keratocyte densities (p > 0.05). However, patients with grade 1 had significantly increased nerve fiber density (p = 0.015) and nerve branch density (p = 0.001) than those with grade 2 and grade 3 pterygium. Corneal IVCM may be useful to better understand the corneal microstructure in cases with pterygium. Comprehensive research on the subject is needed.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"100-105"},"PeriodicalIF":1.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIFU induces reprogramming of the tumor immune microenvironment in a pancreatic cancer mouse model. HIFU诱导胰腺癌小鼠模型中肿瘤免疫微环境的重编程。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2025-01-28 DOI: 10.1007/s00795-025-00419-1

Toshihiro Yamaguchi, Shuji Kitahara, Aya Matsui, Jun Okamoto, Yoshihiro Muragaki, Ken Masamune

引用次数: 0