Medical Molecular Morphology最新文献_第10页

Exploring the impact of ovariectomy on hair growth: can ovariectomized mouse serve as a model for investigating female pattern hair loss in humans? 探索卵巢切除对毛发生长的影响:切除卵巢的小鼠能否作为研究人类女性型脱发的模型?

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-04-29 DOI: 10.1007/s00795-022-00320-1

S. Togo, H. Imanishi, M. Hayashi, M. Koyama, Y. Kira, K. Sugawara, D. Tsuruta

引用次数: 0

Induced pluripotent stem cells from homozygous Runx2-deficient mice show poor response to vitamin D during osteoblastic differentiation 来自纯合子runx2缺陷小鼠的诱导多能干细胞在成骨细胞分化过程中对维生素D的反应较差

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-04-23 DOI: 10.1007/s00795-022-00317-w

H. Aoki, E. Suzuki, Takashi Nakamura, Shoko Onodera, A. Saito, M. Ohtaka, M. Nakanishi, K. Nishimura, Atsushi Saito, Toshifumi Azuma

引用次数: 0

A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy 使用扫描电子显微镜对温度反应培养皿产生的脂肪来源干细胞片进行形态学研究

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-04-21 DOI: 10.1007/s00795-022-00319-8

Yasuhiko Taki, Atsushi Fuku, Yuka Nakamura, Terutsugu Koya, H. Kitajima, Ikuhiro Tanida, T. Takaki, Kaori Nozaki, H. Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Takeo Shimasaki, Togen Masauji, Naoki Yamamoto, Y. Ishigaki, S. Shimodaira, Yusuke Shimizu, T. Ichiseki, A. Kaneuji, S. Osawa, N. Kawahara

引用次数: 1

Microvascular system of hip joint constituents with special reference to ultrastructural findings and early arteriosclerosis 微血管系统的髋关节成分，特别参考超微结构的发现和早期动脉硬化

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-04 DOI: 10.1007/s00795-022-00316-x

N. Kaku, T. Shimada, Tsuguaki Hosoyama, H. Tsumura

引用次数: 0

Association between mitochondrial and nuclear DNA damages and cellular senescence in the patients with biliary atresia undergoing Kasai portoenterostomy and liver transplantation 行Kasai门肠造口术和肝移植的胆道闭锁患者线粒体和核DNA损伤与细胞衰老的关系

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-03 DOI: 10.1007/s00795-022-00314-z

Yudai Nakajima, Yuto Yamazaki, Xin Gao, Masatoshi Hashimoto, M. Nio, M. Wada, F. Fujishima, H. Sasano

引用次数: 1

Mitochonic acid-5 ameliorates chlorhexidine gluconate-induced peritoneal fibrosis in mice. 线粒体酸-5改善葡萄糖酸氯己定诱导的小鼠腹膜纤维化。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-01 Epub Date: 2021-10-07 DOI: 10.1007/s00795-021-00305-6

Hiro Inoue, Kenta Torigoe, Miki Torigoe, Kumiko Muta, Yoko Obata, Takehiro Suzuki, Chitose Suzuki, Takaaki Abe, Takehiko Koji, Hiroshi Mukae, Tomoya Nishino

{"title":"Mitochonic acid-5 ameliorates chlorhexidine gluconate-induced peritoneal fibrosis in mice.","authors":"Hiro Inoue, Kenta Torigoe, Miki Torigoe, Kumiko Muta, Yoko Obata, Takehiro Suzuki, Chitose Suzuki, Takaaki Abe, Takehiko Koji, Hiroshi Mukae, Tomoya Nishino","doi":"10.1007/s00795-021-00305-6","DOIUrl":"https://doi.org/10.1007/s00795-021-00305-6","url":null,"abstract":"Peritoneal fibrosis is a serious complication of long-term peritoneal dialysis, attributable to inflammation and mitochondrial dysfunction. Mitochonic acid-5 (MA-5), an indole-3-acetic acid derivative, improves mitochondrial dysfunction and has therapeutic potential against various diseases including kidney diseases. However, whether MA-5 is effective against peritoneal fibrosis remains unclear. Therefore, we investigated the effect of MA-5 using a peritoneal fibrosis mouse model. Peritoneal fibrosis was induced in C57BL/6 mice via intraperitoneal injection of chlorhexidine gluconate (CG) every other day for 3 weeks. MA-5 was administered daily by oral gavage. The mice were divided into control, MA-5, CG, and CG + MA-5 groups. Following treatment, immunohistochemical analyses were performed. Fibrotic thickening of the parietal peritoneum induced by CG was substantially attenuated by MA-5. The number of α-smooth muscle actin-positive myofibroblasts, transforming growth factor β-positive cells, F4/80-positive macrophages, monocyte chemotactic protein 1-positive cells, and 4-hydroxy-2-nonenal-positive cells was considerably decreased. In addition, reduced ATP5a1-positive and uncoupling protein 2-positive cells in the CG group were notably increased by MA-5. MA-5 may ameliorate peritoneal fibrosis by suppressing macrophage infiltration and oxidative stress, thus restoring mitochondrial function. Overall, MA-5 has therapeutic potential against peritoneal fibrosis.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39496475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MiR-98-3p regulates ovarian granulosa cell proliferation and apoptosis in polycystic ovary syndrome by targeting YY1. MiR-98-3p通过靶向YY1调控多囊卵巢综合征卵巢颗粒细胞增殖和凋亡。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-01 Epub Date: 2021-11-18 DOI: 10.1007/s00795-021-00307-4

Min Hu, Tian Gao, Ying Du

{"title":"MiR-98-3p regulates ovarian granulosa cell proliferation and apoptosis in polycystic ovary syndrome by targeting YY1.","authors":"Min Hu, Tian Gao, Ying Du","doi":"10.1007/s00795-021-00307-4","DOIUrl":"https://doi.org/10.1007/s00795-021-00307-4","url":null,"abstract":"Polycystic ovary syndrome (PCOS) is a common endocrinopathy related to female infertility. We investigated the function of the microRNA-98-3p (miR-98-3p)/Yin-Yang-1 (YY1) axis to the pathophysiological processes in PCOS mice. A mouse model of PCOS was established using dehydroepiandrosterone (DHEA). Hematoxylin and eosin (HE) staining was used to assess morphologic changes of the ovaries. Hormonal serum levels were measured by ELISA. Estrogen synthesis in OGCs was measured using chemiluminescence immunoassay. The viability, cell cycle, and apoptosis of ovarian granulosa cells (OGCs) were assessed by CCK-8, flow cytometry, and western blot. Luciferase reporter assays were conducted to examine the binding of miR-98-3p to YY1. YY1 was upregulated, while miR-98-3p was downregulated both in the ovarian tissues of PCOS mice and OGCs separated from PCOS mice and patients. YY1 Knockdown promoted OGC proliferation and inhibited apoptosis as well as increased estrogen production in OGCs. YY1 was verified to be targeted by miR-98-3p. Additionally, YY1 overexpression prevented the effects of miR-98-3p overexpression on the proliferation and apoptosis of OGCs. Importantly, miR-98-3p attenuated ovarian injury in PCOS mice. MiR-98-3p targets and downregulates YY1 expression, thereby affecting the proliferation and apoptosis of OGCs in PCOS.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Role of repressed microRNAs in endometriosis. 抑制microrna在子宫内膜异位症中的作用。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-01 Epub Date: 2021-08-31 DOI: 10.1007/s00795-021-00303-8

Kaei Nasu, Yoko Aoyagi, Ruofei Zhu, Mamiko Okamoto, Mitsutake Yano, Kentaro Kai, Yasushi Kawano

引用次数: 4

A novel parotid carcinoma with a prominent ghost cell population: a masquerading tumor or "salivary ghost cell carcinoma"? 新型腮腺癌伴明显的鬼细胞群:是伪装肿瘤还是“唾液鬼细胞癌”?

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-01 Epub Date: 2021-08-14 DOI: 10.1007/s00795-021-00302-9

Hiroshi Harada, Mitsuo P Sato, Naoki Otsuki, Mao Kawamura, Akira Kurose, Takao Satou

{"title":"A novel parotid carcinoma with a prominent ghost cell population: a masquerading tumor or \"salivary ghost cell carcinoma\"?","authors":"Hiroshi Harada, Mitsuo P Sato, Naoki Otsuki, Mao Kawamura, Akira Kurose, Takao Satou","doi":"10.1007/s00795-021-00302-9","DOIUrl":"https://doi.org/10.1007/s00795-021-00302-9","url":null,"abstract":"Ghost cell is one of several unique cellular morphologies associated with aberrant keratinization. We encountered a novel parotid tumor containing numerous ghost cells and herein describe its histological features and discuss diagnostic problems. The patient was a 90-year-old Japanese male, who complained of swelling of the left parotid area for four months. Positron emission tomography indicated no cervical lymph node metastasis or distant metastasis. The tumor was successfully resected with no signs of recurrence or metastasis for six months after surgery. Histologically, the tumor was mainly composed of squamous cells forming irregularly shaped nests with a mixture of pleomorphic giant or multinucleated cells and bland basaloid cell. Keratinized areas were occupied by a prominent ghost cell population. Immunohistochemically, CK5/6 and CK19 were widely positive as well as AE1/AE3, p40 and p63. Nuclear expression of β-catenin was also observed. The present case can be regarded as a particular form of squamous cell carcinoma and is believed to contain a large number of ghost cells resulting from an unclear mechanism. However, it seems difficult to consider such tumors as a clinicopathologically independent entity at present. Applying a term such as \"salivary ghost cell carcinoma\" would be premature.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00795-021-00302-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39322508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The concurrent stimulation of Wnt and FGF8 signaling induce differentiation of dental mesenchymal cells into odontoblast-like cells. 同时刺激Wnt和FGF8信号可诱导牙间充质细胞向成牙细胞样细胞分化。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-03-01 Epub Date: 2021-11-05 DOI: 10.1007/s00795-021-00297-3

Motoyoshi Kimura, Akiko Saito, Shoko Onodera, Takashi Nakamura, Makoto Suematsu, Seikou Shintani, Toshifumi Azuma

{"title":"The concurrent stimulation of Wnt and FGF8 signaling induce differentiation of dental mesenchymal cells into odontoblast-like cells.","authors":"Motoyoshi Kimura, Akiko Saito, Shoko Onodera, Takashi Nakamura, Makoto Suematsu, Seikou Shintani, Toshifumi Azuma","doi":"10.1007/s00795-021-00297-3","DOIUrl":"https://doi.org/10.1007/s00795-021-00297-3","url":null,"abstract":"Fibroblast growth factor 8 (FGF8) is known to be a potent stimulator of canonical Wnt/β-catenin activity, an essential factor for tooth development. In this study, we analyzed the effects of co-administration of FGF8 and a CHIR99021 (GSK3β inhibitor) on differentiation of dental mesenchymal cells into odontoblasts. Utilizing Cre-mediated EGFP reporter mice, dentin matrix protein 1 (Dmp1) expression was examined in mouse neonatal molar tooth germs. At birth, expression of Dmp1-EGFP was not found in mesenchymal cells but rather epithelial cells, after which Dmp1-positive cells gradually emerged in the mesenchymal area along with disappearance in the epithelial area. Primary cultured mesenchymal cells from neonatal tooth germ specimens showed loss of Dmp1-EGFP positive signals, whereas addition of Wnt3a or the CHIR99021 significantly regained Dmp1 positivity within approximately 2 weeks. Other odontoblast markers such as dentin sialophosphoprotein (Dspp) could not be clearly detected. Concurrent stimulation of primary cultured mesenchymal cells with the CHIR99021 and FGF8 resulted in significant upregulation of odonto/osteoblast proteins. Furthermore, increased expression levels of runt-related transcription factor 2 (Runx2), osterix, and osteocalcin were also observed. The present findings indicate that coordinated action of canonical Wnt/β-catenin and FGF8 signals is essential for odontoblast differentiation of tooth germs in mice.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39681942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5