Medical Molecular Morphology最新文献_第2页

Three-dimensional visualization of calcification during scale regeneration in goldfish. 金鱼鳞片再生过程中钙化的三维可视化。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-09-01 Epub Date: 2025-03-06 DOI: 10.1007/s00795-025-00427-1

Hisayuki Funahashi, Yusuke Maruyama, Nobuo Suzuki, Takashi Takaki, Kazuho Honda, Atsuhiko Hattori

引用次数: 0

Keratin (OSCAR) immunohistochemistry as a reliable diagnostic marker for anaplastic thyroid carcinoma. 角蛋白（OSCAR）免疫组化作为间变性甲状腺癌的可靠诊断标志物。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-08-26 DOI: 10.1007/s00795-025-00447-x

Rin Yamada, Daiki Yoshii, Yoshihiro Komohara, Kaori Yukino, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita

引用次数: 0

The role and potential mechanism of immunoglobulin G N-glycosylation in gastrointestinal tumors. 免疫球蛋白G - n -糖基化在胃肠道肿瘤中的作用及其潜在机制。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-08-25 DOI: 10.1007/s00795-025-00448-w

Yumeng Liu, Zejian Zhang, Xiequn Xu

{"title":"The role and potential mechanism of immunoglobulin G N-glycosylation in gastrointestinal tumors.","authors":"Yumeng Liu, Zejian Zhang, Xiequn Xu","doi":"10.1007/s00795-025-00448-w","DOIUrl":"https://doi.org/10.1007/s00795-025-00448-w","url":null,"abstract":"Gastrointestinal tumors significantly contribute to cancer-related mortality worldwide. Early detection coupled with effective treatment significantly improves overall survival. Immunoglobulin G (IgG) N-glycosylation, a crucial post-translational modification, undergoes alterations in glycan structures. IgG N-glycosylation is associated with numerous physiological and pathological processes in the human body. Aberrant changes of IgG N-glycosylation play a key role in cancers given the involvement of glycans in cancer progression and immune modulation. These changes affect the binding of the Fc region of IgG to its receptor, in turn, affect the corresponding downstream effects, which are crucial in cancer immuno-surveillance and immune escape. This review aims to explore the latest advancements in understanding IgG N-glycosylation in gastrointestinal cancers, emphasizing its potential as a diagnostic biomarker and therapeutic target. The application of IgG N-glycosylation in clinical oncology could enhance early detection, improve therapeutic efficacy, and enable better monitoring of disease progression and recurrence. Furthermore, we summarized the research progression to provide novel insights into the potential regulatory mechanism of IgG N-glycosylation in gastrointestinal tumors. In all, IgG N-glycosylation holds significant promise for advancing cancer diagnosis and treatment. Further studies are required to fully elucidate its mechanisms and optimize its use in clinical practice.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epithelioid sarcoma originating in the chest wall: A case report. 起源于胸壁的上皮样肉瘤1例。

IF 1.1 4区医学

Medical Molecular Morphology Pub Date : 2025-08-09 DOI: 10.1007/s00795-025-00445-z

Yuzo Oyama, Riko Furukawa-Kubota, Hiroko Kadowaki, Junnpei Wada, Kazuhiro Kawamura, Takashi Miura, Tsutomu Daa

{"title":"Epithelioid sarcoma originating in the chest wall: A case report.","authors":"Yuzo Oyama, Riko Furukawa-Kubota, Hiroko Kadowaki, Junnpei Wada, Kazuhiro Kawamura, Takashi Miura, Tsutomu Daa","doi":"10.1007/s00795-025-00445-z","DOIUrl":"https://doi.org/10.1007/s00795-025-00445-z","url":null,"abstract":"This report presents the sixth case of chest wall epithelioid sarcoma (ES) in a 71-year-old Japanese man. The patient was incidentally diagnosed with a soft tissue tumor between the eighth and ninth ribs, presenting with an associated bone fracture and osteolytic change. Marginal resection followed by chest wall reconstruction was performed for a definitive diagnosis. Histopathological examination revealed multinodular growth associated with collagenous stroma, mimicking a necrotizing granulomatous process. Various tumor cells were observed, including epithelioid, spindle-shaped, and rhabdoid cells. Immunohistochemical analysis, conducted on trimmed tumor samples without decalcification, revealed positivity for cytokeratin AE1/AE3, vimentin, and CD34, as well as negativity for CK7, CK20, CD31, calretinin, and D2-40. INI expression was completely absent in tumor cells. The patient was diagnosed with ES. The chest wall is an unusual location for ES, and its diagnosis requires differentiation from other epithelioid neoplasms. This case highlights the importance of trimming tumor samples before decalcification to preserve antigenicity and ensure accurate immunohistochemistry analysis.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anaplastic thyroid carcinoma with osteoclast-like giant cells: a case report and a study of a potential therapeutic approach. 甲状腺间变性癌伴破骨细胞样巨细胞：一例报告及一种潜在治疗方法的研究。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-07-24 DOI: 10.1007/s00795-025-00443-1

Kaori Yukino, Yoshihiro Komohara, Shukang Zhao, Rin Yamada, Yukio Fujiwara, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita

{"title":"Anaplastic thyroid carcinoma with osteoclast-like giant cells: a case report and a study of a potential therapeutic approach.","authors":"Kaori Yukino, Yoshihiro Komohara, Shukang Zhao, Rin Yamada, Yukio Fujiwara, Akira Murakami, Yu Shimoda, Haruki Saito, Yorihisa Orita","doi":"10.1007/s00795-025-00443-1","DOIUrl":"https://doi.org/10.1007/s00795-025-00443-1","url":null,"abstract":"Anaplastic thyroid carcinoma (ATC) is a highly aggressive neoplasm with no effective treatment options. ATC with osteoclast-like giant cells (OGCs; ATC/OGC) is a rare variant of ATC, and no detailed pathological examination has been reported to date. A 59-year-old woman presented with sudden neck swelling. Computed tomography revealed a 5 cm tumor in the thyroid gland, which was surgically resected. Pathological examination revealed a diagnosis of ATC/OGC. The patient succumbed to progressive lung metastases within four months despite postoperative lenvatinib therapy. Immunohistochemical (IHC) examination indicated absence of PD-L1 expression in the OGCs, which comprised the majority of the tumor, with only sparse T cell infiltration in the area occupied by OGCs. Increased TGF-β expression was observed in the area containing OGCs, and both OGCs and infiltrating myeloid cells, including CD1a/CD11c-positive dendritic cells and CD68/CD163/CD204-positive macrophages, appeared to produce TGF-β. Pathological analysis of this case suggests that OGCs might be involved in immune suppression by secreting TGF-β, potentially serving as a critical cytokine in the immunosuppressive microenvironment of ATC/OGC. TGF-β-targeted therapy might be useful in the treatment of this very rare subtype of ATC.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs. 马拉塞人上皮细胞克隆细胞条件培养基对牙胚牙釉质结晶的影响。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-07-07 DOI: 10.1007/s00795-025-00444-0

Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh

{"title":"Effect of conditioned medium derived from a clone cell of epithelial rests of Malassez on enamel crystallization in tooth germs.","authors":"Dembereldorj Bolortsetseg, Yoshihito Kurashige, Maria Mielnik-Błaszczak, Syed Taufiqul Islam, Yusuke Fujita, Sayaka Sakakibara, Erika Minowa, Hiroyo Yoshimoto, Yoshihiro Abiko, Masato Saitoh","doi":"10.1007/s00795-025-00444-0","DOIUrl":"https://doi.org/10.1007/s00795-025-00444-0","url":null,"abstract":"We have previously isolated the epithelial rests of Malassez (ERM) clone cells with strong Amelx expression, named as ERM-2, from the crude ERM cells. In the present study, we examined whether conditioned medium (CM) derived from cultured ERM-2 promotes the crystallization of immature enamel in tooth germs. Tooth germs from postnatal day 3 mice were incubated with ERM-2 conditional medium (CM). ERM-2 cells were transfected with si-RNA targeting specific enamel matrix proteins (EMPs). After 2 days of incubation, each CM was collected and employed to culture the tooth germs. The surface layers of the enamel structure were examined with a scanning electron microscope (SEM). Tooth germs cultured with ERM-2 CM on days 3 and 7 showed elongation and densification of the columnar structures in SEM analysis. The columnar structures became denser and aggregated forming a HAP-like hexagonal columnar structure 14 days after culture in ERM-2 CM. In contrast, no clear columnar structures were observed in ERM-2 CM with si-RNA of each EMPs. In conclusion, the present study demonstrated that CM derived from ERM-2 could form enamel-like structures on the surface of the tooth germ. ERM-2 may provide the possibility for the clinical use of enamel regeneration.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat stroke-induced hepatic lipid dysregulation: histological and lipidomic insights. 中暑引起的肝脂质失调：组织学和脂质组学的见解。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-07-02 DOI: 10.1007/s00795-025-00441-3

Takahiro Deguchi, Hiroki Tanaka, Kie Horioka, Chihiro Matsuhisa, Akira Hayakawa, Shuhei Takauji, Shimpei Watanabe, Masanori Goto, Yumiko Fujii, Kumi Takasawa, Akira Takasawa

{"title":"Heat stroke-induced hepatic lipid dysregulation: histological and lipidomic insights.","authors":"Takahiro Deguchi, Hiroki Tanaka, Kie Horioka, Chihiro Matsuhisa, Akira Hayakawa, Shuhei Takauji, Shimpei Watanabe, Masanori Goto, Yumiko Fujii, Kumi Takasawa, Akira Takasawa","doi":"10.1007/s00795-025-00441-3","DOIUrl":"https://doi.org/10.1007/s00795-025-00441-3","url":null,"abstract":"Global warming has increased summer temperatures, leading to a rise in heat stroke-related deaths in Japan. Heat stroke disrupts the body's adaptation to high temperatures, often resulting in severe complications, including liver damage and even death. However, despite the increasing incidence, pathological autopsies remain rare, and the histological changes associated with heat stroke are poorly understood. In this study, we investigated the pathogenesis of heat stroke using a mouse model. Mice were exposed to 45 °C for 30 min and dissected immediately or 24, 48, and 72 h post-exposure. Histological analysis revealed significant lipid accumulation in hepatocytes surrounding the central vein at 24, 48, and 72 h. At 24 h, hepatocytes also exhibited features of early degeneration, including cytoplasmic lysis and chromatin condensation. Lipidomics analysis of liver tissue collected 24 h post-exposure demonstrated a marked increase in 27-hydroxycholesterol levels. These results indicate that heat stress rapidly disrupts hepatic lipid homeostasis, causing cellular damage and metabolic remodeling. The observed lipid accumulation, including elevated 27-hydroxycholesterol, may play dual roles in mediating inflammation and serving as a protective response. Our findings provide new insight into the pathogenesis of heat stroke-induced liver injury and suggest potential molecular targets for early diagnosis and intervention.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors. 免疫组织化学和分子方法评价PRDM10基因重排在无法分类的未分化软组织肿瘤中的作用。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-25 DOI: 10.1007/s00795-025-00442-2

Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen

{"title":"Evaluation of PRDM10 gene rearrangement by immunohistochemistry and molecular methods in unclassifiable undifferentiated soft tissue tumors.","authors":"Merve Aksin, Kivilcim Eren Ates, Akif Mirioglu, Tugba Toyran, Gulfiliz Gonlusen","doi":"10.1007/s00795-025-00442-2","DOIUrl":"https://doi.org/10.1007/s00795-025-00442-2","url":null,"abstract":"Soft tissue sarcomas are heterogenous groups of tumors that show variable morphology as well as clinical behavior. Morphological features do not always directly reflect clinical behavior. Certain mesenchymal tumors exhibit an indolent clinical course. Among them are superficial CD34-positive fibroblastic tumors characterized by PRDM10 fusion. In our study, we aimed to detect PRDM10 gene rearrangement in superficial CD34-positive fibroblastic tumors and other pleomorphic sarcomas included in its differential diagnosis by immunohistochemistry and Fluorescence in situ hybridization. Totally, 33 cases were enrolled into this study. The results showed that two cases diagnosed as superficial CD34-positive fibroblastic tumor and two cases diagnosed as undifferentiated pleomorphic sarcoma have PRDM10 gene rearrangement. Immunohistochemically, not all rearranged tumors showed PRDM10 staining that suggests a low sensitivity of PRDM10 antibody. In conclusion, we suggested that PRDM10 gene rearrangement is not limited to superficial CD34-positive fibroblastic tumors; undifferentiated pleomorphic sarcomas may exhibit this molecular alteration and immunohistochemistry has lower sensitivity than fluorescence in situ hybridization.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies. 代谢功能障碍相关脂肪变性肝病活检中肝纤维化模式对肝癌发生的预测

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-05 DOI: 10.1007/s00795-025-00440-4

Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao

{"title":"Hepatocarcinogenesis prediction by liver fibrosis patterns in metabolic dysfunction-associated steatotic liver disease biopsies.","authors":"Asami Beppu, Hisamitsu Miyaaki, Satoshi Miuma, Ryu Sasaki, Masafumi Haraguchi, Masanori Fukusima, Yasuhiko Nakao, Kazuaki Tajima, Satoshi Matsuo, Yuko Akazawa, Shinji Okano, Kazuhiko Nakao","doi":"10.1007/s00795-025-00440-4","DOIUrl":"https://doi.org/10.1007/s00795-025-00440-4","url":null,"abstract":"This study aimed to investigate carcinogenesis-related fibrosis patterns in liver biopsy tissues from patients with metabolic dysfunction-associated steatotic liver disease (MASLD) by comprehensively measuring and quantifying various fibrosis patterns using artificial intelligence. Liver biopsy tissues from 13 patients with advanced fibrosis at MASLD diagnosis were subjected to collagen quantification and morphological and structural fiber characteristic evaluation using FibroNest (PharmaNest, Princeton, NJ, USA), which was described using up to seven quantitative fibrosis parameters (qFPs). The collagen-fibrosis composite score (FCS), morphometric-FCS, architecture-FCS, and phenotypic-FCS (Ph-FCS) were compared between patients with and without hepatocellular carcinoma (HCC). The collagen quantification alone could not discriminate between HCC and non-HCC cases. Regarding the individual qFPs of morphological fiber characteristics, the kurtosis and skewness of fiber twists were significantly lower in HCC cases than in non-HCC cases. In HCC cases, fiber width and density kurtosis tended to be larger, whereas fiber length kurtosis tended to be smaller than those in non-HCC cases. Ph-FCS could discriminate HCC from non-HCC at a threshold of 4.2, with 85% sensitivity and 100% specificity. A combination of fiber morphology and structural characteristics predicted HCC development with higher accuracy and might help define carcinogenic risk groups among patients with MASLD.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relation in MreB and intrabacterial nanotransportation system for VacA in Helicobacter pylori. 幽门螺旋杆菌中的 MreB 与 VacA 的细菌内纳米运输系统的关系。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2025-06-01 Epub Date: 2024-12-20 DOI: 10.1007/s00795-024-00416-w

Hong Wu, Yoshihiko Fujioka, Noritaka Iwai, Shoichi Sakaguchi, Youichi Suzuki, Takashi Nakano

引用次数: 0