Mass spectrometry最新文献_第9页

Development of Non-proximate Probe Electrospray Ionization for Real-Time Analysis of Living Animal. 用于活体动物实时分析的非近似探针电喷雾电离技术的发展。

Mass spectrometry Pub Date : 2015-02-01 DOI: 10.5702/massspectrometry.S0048

K. Yoshimura, L. Chen, H. Johno, Mayutaka Nakajima, K. Hiraoka, S. Takeda

{"title":"Development of Non-proximate Probe Electrospray Ionization for Real-Time Analysis of Living Animal.","authors":"K. Yoshimura, L. Chen, H. Johno, Mayutaka Nakajima, K. Hiraoka, S. Takeda","doi":"10.5702/massspectrometry.S0048","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0048","url":null,"abstract":"Ambient ionization mass spectrometry is one of the most challenging analytical tools in the field of biomedical research. We previously demonstrated that probe electrospray ionization mass spectrometry (PESI-MS) could potentially be used in the rapid diagnosis of cancer. Although this technique does not require a tedious sample pretreatment process, it was not possible for our previously reported setup to be applied to cases involving the direct sampling of tissues from living animal and large animal subjects, because there would not be enough room to accommodate the larger bodies juxtaposed to the ion inlet. To make PESI-MS more applicable for the real-time analysis of living animals, a long auxiliary ion sampling tube has been connected to the ion inlet of the mass spectrometer to allow for the collection of ions and charged droplets from the PESI source (hereafter, referred to as non-proximate PESI). Furthermore, an additional ion sampling tube was connected to a small diaphragm pump to increase the uptake rate of air carrying the ions and charged droplets to the ion inlet. This modification allows for the extended ion sampling orifice to be positioned closer to the specimens, even when they are too large to be placed inside the ionization chamber. In this study, we have demonstrated the use of non-proximate PESI-MS for the real-time analysis for biological molecules and pharmacokinetic parameters from living animals.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"2 1","pages":"S0048"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88705150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Multi-Component Profiling of Trace Volatiles in Blood by Gas Chromatography/Mass Spectrometry with Dynamic Headspace Extraction. 动态顶空萃取气相色谱/质谱法分析血液中痕量挥发物。

Mass spectrometry Pub Date : 2015-02-01 DOI: 10.5702/massspectrometry.A0034

Shoji Kakuta, Toshiyuki Yamashita, S. Nishiumi, Masaru Yoshida, E. Fukusaki, T. Bamba

{"title":"Multi-Component Profiling of Trace Volatiles in Blood by Gas Chromatography/Mass Spectrometry with Dynamic Headspace Extraction.","authors":"Shoji Kakuta, Toshiyuki Yamashita, S. Nishiumi, Masaru Yoshida, E. Fukusaki, T. Bamba","doi":"10.5702/massspectrometry.A0034","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0034","url":null,"abstract":"A dynamic headspace extraction method (DHS) with high-pressure injection is described. This dynamic extraction method has superior sensitivity to solid phase micro extraction, SPME and is capable of extracting the entire gas phase by purging the headspace of a vial. Optimization of the DHS parameters resulted in a highly sensitive volatile profiling system with the ability to detect various volatile components including alcohols at nanogram levels. The average LOD for a standard volatile mixture was 0.50 ng mL(-1), and the average LOD for alcohols was 0.66 ng mL(-1). This method was used for the analysis of volatile components from biological samples and compared with acute and chronic inflammation models. The method permitted the identification of volatiles with the same profile pattern as in vitro oxidized lipid-derived volatiles. In addition, the concentration of alcohols and aldehydes from the acute inflammation model samples were significantly higher than that for the chronic inflammation model samples. The different profiles between these samples could also be identified by this method. Finally, it was possible to analyze alcohols and low-molecular-weight volatiles that are difficult to analyze by SPME in high sensitivity and to show volatile profiling based on multi-volatile simultaneous analysis.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"4 3 1","pages":"A0034"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79473923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Recent Progress in Presolar Grain Studies. 前太阳谷物研究进展。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0042

S. Amari

引用次数: 2

Complementary Characterization of End Groups in Radically Polymerized Poly(methyl methacrylate) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Pyrolysis-Gas Chromatography-Mass Spectrometry. 基质辅助激光解吸/电离质谱法和热解-气相色谱-质谱法对自由基聚合聚甲基丙烯酸甲酯端基的互补表征。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0041

H. Ohtani, T. Iura

引用次数: 3

Recent Advance in Liquid Chromatography/Mass Spectrometry Techniques for Environmental Analysis in Japan. 液相色谱/质谱技术在日本环境分析中的最新进展。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0047

Shigeru Suzuki

引用次数: 1

Activation of Nitrogen-Fixing Endophytes Is Associated with the Tuber Growth of Sweet Potato. 固氮内生菌的激活与甘薯块茎生长有关。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.A0032

K. Yonebayashi, Naoya Katsumi, Tomoe Nishi, M. Okazaki

{"title":"Activation of Nitrogen-Fixing Endophytes Is Associated with the Tuber Growth of Sweet Potato.","authors":"K. Yonebayashi, Naoya Katsumi, Tomoe Nishi, M. Okazaki","doi":"10.5702/massspectrometry.A0032","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0032","url":null,"abstract":"Endophytic nitrogen-fixing organisms have been isolated from the aerial parts of field-grown sweet potato (Ipomoea batatas). The (15)N dilution method, which is based on the differences in stable nitrogen isotope ratios, is useful for measuring nitrogen fixation in the field. In this study, seedlings of two sweet potato cultivars, 'Beniazuma' and 'Benikomachi,' were transplanted into an alluvial soil that had been treated with organic improving material in advance. Whole plants were sampled every 2 or 3 weeks. After separating plants into tuberous roots and leaves, the fresh weights of the samples were measured, and the nitrogen content and natural (15)N content of leaves were determined with an elemental analyzer and an isotope ratio mass spectrometer linked to an elemental analyzer, respectively. The contribution of nitrogen fixation derived from atmospheric N2 in sweet potato was calculated by assuming that leaves at 2 weeks after transplanting were in a non-nitrogen-fixing state. The contribution ratios of nitrogen fixation by nitrogen-fixing endophytes in leaves of both sweet potato cultivars increased rapidly from 35 to 61 days after transplanting and then increased gradually to 55-57% at 90 days after transplanting. Over the course of the sweet potato growing season, the activity of nitrogen-fixing endophytes in leaves began to increase at about 47 days after transplanting, the weight of leaves increased rapidly, and then growth of tuberous roots began a few weeks later. Our findings indicate that nitrogen-fixing endophytes will be activated under inorganic nitrogen-free sweet potato cultivation, allowing for growth of the tuberous roots.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"14 1","pages":"A0032"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83298756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

High-Throughput Analysis of Sucrose Fatty Acid Esters by Supercritical Fluid Chromatography/Tandem Mass Spectrometry. 超临界流体色谱/串联质谱法高通量分析蔗糖脂肪酸酯。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.A0033

Katsuhito Hori, K. Tsumura, E. Fukusaki, T. Bamba

引用次数: 4

Establishment of a Strategy for the Discovery and Verification of Low-Abundance Biomarker Peptides in Plasma Using Two Types of Stable-Isotope Tags. 利用两种稳定同位素标签建立血浆中低丰度生物标记肽的发现和验证策略。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0044

Y. Kodera, Yuya Hido, Rika Kato, Tatsuya Saito, Y. Kawashima, Satoru Minamida, Kazumasa Matsumoto, M. Iwamura

{"title":"Establishment of a Strategy for the Discovery and Verification of Low-Abundance Biomarker Peptides in Plasma Using Two Types of Stable-Isotope Tags.","authors":"Y. Kodera, Yuya Hido, Rika Kato, Tatsuya Saito, Y. Kawashima, Satoru Minamida, Kazumasa Matsumoto, M. Iwamura","doi":"10.5702/massspectrometry.S0044","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0044","url":null,"abstract":"Serum and plasma contain thousands of different proteins and peptides, which can provide valuable information about the numerous processes that take place within the body. However, detailed analysis of proteins and peptides in serum and plasma remains challenging due to the presence of many high-abundance proteins, the large dynamic range of protein and peptide concentrations, the extensive complexity caused by posttranslational modifications, and considerable individual variability. In particular, detailed analysis and identification of native peptides is extremely difficult due to the tremendous variety of cleavage possibilities and posttranslational modifications, which results in extremely high complexity. Therefore, widely ranging searches based on peptide identification are difficult. Herein, we describe the highly accurate and sensitive quantitative analysis of over 2,500 peptides with the concentration limit of about 10 pM. The strategy combined isobaric tag labeling, amine-reactive 6-plex tandem mass tag labeling, and a modified differential solubilization method for high-yield peptide extraction [Saito, T. et al. J. Electrophoresis 2013 57: 1-9]. Using this strategy, we quantitatively analyzed six pooled plasma samples (three pre-surgery and three post-surgery) to discover potential candidate biomarker peptides of renal cell carcinoma. The concentrations of 27 peptides were found to be altered following surgery. A preliminary validation study was conducted using about 80 plasma samples to demonstrate the possibility that even unidentified potential candidate biomarker peptides can be verified using the isotope tag/dimethyl labeling method. We also discuss technical consideration and potential of this strategy for facilitating native peptide research.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"10 1","pages":"S0044"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84202337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Application of Ion Mobility-Mass Spectrometry to the Study of Ionic Clusters: Investigation of Cluster Ions with Stable Sizes and Compositions. 离子迁移-质谱法在离子团簇研究中的应用:具有稳定大小和组成的团簇离子的研究。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0043

K. Ohshimo, T. Komukai, Tohru Takahashi, Naoya Norimasa, Jenna W. J. Wu, R. Moriyama, K. Koyasu, F. Misaizu

引用次数: 4

Application of Metabolomics for High Resolution Phenotype Analysis. 代谢组学在高分辨率表型分析中的应用。

Mass spectrometry Pub Date : 2014-12-01 DOI: 10.5702/massspectrometry.S0045

E. Fukusaki

{"title":"Application of Metabolomics for High Resolution Phenotype Analysis.","authors":"E. Fukusaki","doi":"10.5702/massspectrometry.S0045","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0045","url":null,"abstract":"Metabolome, a total profile of whole metabolites, is placed on downstream of proteome. Metabolome is thought to be results of implementation of genomic information. In other words, metabolome can be called as high resolution phenotype. The easiest operation of metabolomics is the integration to the upstream ome information including transcriptome and/or proteome. Those trials have been reported at a certain scientific level. In addition, metabolomics can be operated in stand-alone mode without any other ome information. Among metabolomics tactics, the author's group is particularly focusing on metabolic fingerprinting, in which metabolome information is employed as explanatory variant to evaluate response variant. Metabolic fingerprinting technique is expected not only for analyzing slight difference depending on genotype difference but also for expressing dynamic variation of living organisms. The author introduces several good examples which he performed. Those are useful for easy understanding of the power of metabolomics. In addition, the author mentions the latest technology for analysis of metabolic dynamism. The author's group developed a facile analytical method for semi-quantitative metabolic dynamism. The author introduces the novel method that uses time dependent variation of isotope distribution based on stable isotope dilution.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"51 1","pages":"S0045"},"PeriodicalIF":0.0,"publicationDate":"2014-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76649258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10