Mass spectrometryPub Date : 2019-06-24DOI: 10.1002/9781119377368.ch8
V. Cunsolo, S. Foti, J. Ner‐Kluza, A. Drabik, J. Silberring, V. Muccilli, R. Saletti, Katarzyna Pawlak, E. Harwood, Fang Yu, P. Ciborowski, R. Anczkiewicz, Kathrin Altweg, G. Spoto, A. Pawlaczyk, M. Szynkowska, M. Smoluch, D. Kwiatkowska
{"title":"Mass Spectrometry Applications","authors":"V. Cunsolo, S. Foti, J. Ner‐Kluza, A. Drabik, J. Silberring, V. Muccilli, R. Saletti, Katarzyna Pawlak, E. Harwood, Fang Yu, P. Ciborowski, R. Anczkiewicz, Kathrin Altweg, G. Spoto, A. Pawlaczyk, M. Szynkowska, M. Smoluch, D. Kwiatkowska","doi":"10.1002/9781119377368.ch8","DOIUrl":"https://doi.org/10.1002/9781119377368.ch8","url":null,"abstract":"","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84080121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mass spectrometryPub Date : 2019-06-24DOI: 10.1002/9781119377368.ch5
A. Drabik, F. Bellia, P. Mielczarek, J. Silberring
{"title":"Hyphenated Techniques","authors":"A. Drabik, F. Bellia, P. Mielczarek, J. Silberring","doi":"10.1002/9781119377368.ch5","DOIUrl":"https://doi.org/10.1002/9781119377368.ch5","url":null,"abstract":"","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74627235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fragmentation of Dicarboxylic and Tricarboxylic Acids in the Krebs Cycle Using GC-EI-MS and GC-EI-MS/MS.","authors":"Nobuyuki Okahashi, Shuichi Kawana, Junko Iida, Hiroshi Shimizu, Fumio Matsuda","doi":"10.5702/massspectrometry.A0073","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0073","url":null,"abstract":"<p><p>Isotope labeling measurements using mass spectrometry can provide informative insights on the metabolic systems of various organisms. The detailed identification of carbon positions included in the fragment ions of dicarboxylic and tricarboxylic acids in central carbon metabolism is needed for precise interpretation of the metabolic states. In this study, fragment ions containing the carbon backbone cleavage of dicarboxylic and tricarboxylic in the Krebs cycle were investigated by using gas chromatography (GC)-electron ionization (EI)-MS and GC-EI-MS/MS. The positions of decarboxylation in the dicarboxylic and tricarboxylic acids were successfully identified by analyses using position-specific <sup>13</sup>C-labeled standards prepared by <i>in vitro</i> enzymatic reactions. For example, carboxyl groups of C1 and C6 of trimethylsilyl (TMS)- and <i>tert</i>-butyldimethylsilyl (TBDMS)-derivatized malic and citric acids were primarily cleaved by EI. MS/MS analyses were also performed, and fragment ions of TBDMS-citric and α-ketoglutaric acids (αKG) with the loss of two carboxyl groups in collision-induced dissociation (CID) were observed.</p>","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"8 1","pages":"A0073"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37603703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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