Mass spectrometry最新文献_第8页

A Method for Simultaneous Determination of 25-Hydroxyvitamin D3 and Its 3-Sulfate in Newborn Plasma by LC/ESI-MS/MS after Derivatization with a Proton-Affinitive Cookson-Type Reagent. 质子亲和型cookson试剂衍生后LC/ESI-MS/MS同时测定新生儿血浆中25-羟基维生素D3及其3-硫酸盐的方法

Mass spectrometry Pub Date : 2016-09-02 DOI: 10.5702/massspectrometry.S0051

T. Higashi, M. Yokota, Ayaka Goto, Kenji Komatsu, Takahiro Sugiura, Shoujiro Ogawa, M. Satoh, F. Nomura

{"title":"A Method for Simultaneous Determination of 25-Hydroxyvitamin D3 and Its 3-Sulfate in Newborn Plasma by LC/ESI-MS/MS after Derivatization with a Proton-Affinitive Cookson-Type Reagent.","authors":"T. Higashi, M. Yokota, Ayaka Goto, Kenji Komatsu, Takahiro Sugiura, Shoujiro Ogawa, M. Satoh, F. Nomura","doi":"10.5702/massspectrometry.S0051","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0051","url":null,"abstract":"A method for the simultaneous determination of 25-hydroxyvitamin D3 [25(OH)D3] and its 3-sulfate [25(OH)D3S] in newborn plasma, which is expected to be helpful in the assessment of the vitamin D status, using stable isotope-dilution liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) has been developed and validated. The plasma was pretreated based on the deproteinization and solid-phase extraction, then subjected to derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD). The derivatization enabled the accurate quantification of 25(OH)D3 without interference from 3-epi-25(OH)D3 and also facilitated the simultaneous determination of the two metabolites by LC/positive ESI-MS/MS. Quantification was based on the selected reaction monitoring with the characteristic fragmentation of the DAPTAD-derivatives during MS/MS. This method was reproducible (intra- and inter-assay relative standard deviations of 7.8% or lower for both metabolites) and accurate (analytical recovery, 95.4-105.6%). The limits of quantification were 1.0 ng/mL and 2.5 ng/mL for 25(OH)D3 and 25(OH)D3S, respectively, when using a 20-μL sample. The developed method was applied to the simultaneous determination of plasma 25(OH)D3 and 25(OH)D3S in newborns; it was recognized that the plasma concentration of 25(OH)D3S is significantly higher than that of 25(OH)D3, and preterm newborns have lower plasma 25(OH)D3S concentrations than full-term newborns.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"2016 1","pages":"S0051"},"PeriodicalIF":0.0,"publicationDate":"2016-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88450531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Conifer-Derived Monoterpenes and Forest Walking. 针叶树衍生的单萜烯与森林行走。

Mass spectrometry Pub Date : 2015-10-15 DOI: 10.5702/massspectrometry.A0042

K. Sumitomo, Hiroaki Akutsu, Syusei Fukuyama, A. Minoshima, Shin Kukita, Yuji Yamamura, Yoshiaki Sato, Taiki Hayasaka, Shinobu Osanai, H. Funakoshi, N. Hasebe, Masao Nakamura

引用次数: 23

Development of an Imaging Mass Spectrometry Technique for Visualizing Localized Cellular Signaling Mediators in Tissues. 组织中定位细胞信号介质成像质谱技术的发展。

Mass spectrometry Pub Date : 2015-07-21 DOI: 10.5702/massspectrometry.A0040

Y. Sugiura, Kurara Honda, M. Suematsu

{"title":"Development of an Imaging Mass Spectrometry Technique for Visualizing Localized Cellular Signaling Mediators in Tissues.","authors":"Y. Sugiura, Kurara Honda, M. Suematsu","doi":"10.5702/massspectrometry.A0040","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0040","url":null,"abstract":"In vivo concentrations of cellular signaling mediators such as inflammatory mediators are normally maintained at very low levels due to their strong ability to induce a biological response. The production, diffusion, and decomposition of such mediators are spatio-temporally regulated. Therefore, in order to understand biochemical basis of disease progression and develop new therapeutic strategies, it is important to understand the spatiotemporal dynamics of the signaling mediators in vivo, during the progression of disorders, e.g., chronic inflammatory diseases; however, the lack of effective imaging technology has made it difficult to determine their localizations in vivo. Such characterization requires technical breakthroughs, including molecular imaging methods that are sensitive enough to detect low levels of metabolites in the heterogeneous tissue regions in diseased organs. We and other groups have attempted to fill this technical gap by developing highly sensitive imaging mass spectrometry (IMS) technologies. To date, we have established two key techniques toward this goal, including (i) a sample preparation procedure that has eliminated the problem of the postmortem degradation of labile metabolites, and (ii) on-tissue derivatization of metabolites, which can enhance analyte ionization efficiency. Here, we review recent progress in the development of these technologies as well as how the highly sensitive IMS technique has contributed to increasing understanding of the biochemical basis of disease mechanisms, discovery of new diagnostic markers, and development of new therapies.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"16 1","pages":"A0040"},"PeriodicalIF":0.0,"publicationDate":"2015-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81586495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

When API Mass Spectrometry Meets Super Atmospheric Pressure Ion Sources. 当API质谱法满足超大气压力离子源。

Mass spectrometry Pub Date : 2015-07-07 DOI: 10.5702/massspectrometry.A0041

L. Chen

{"title":"When API Mass Spectrometry Meets Super Atmospheric Pressure Ion Sources.","authors":"L. Chen","doi":"10.5702/massspectrometry.A0041","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0041","url":null,"abstract":"In a tutorial paper on the application of free-jet technique for API-MS, John Fenn mentioned that \"…for a number of years and a number of reasons, it has been found advantageous in many situations to carry out the ionization process in gas at pressures up to 1000 Torr or more\" (Int. J. Mass Spectrom. 200: 459-478, 2000). In fact, the first ESI mass spectrometer constructed by Yamashita and Fenn had a counter-flow curtain gas source at 1050 Torr (ca. 1.4 atm) to sweep away the neutral (J. Phys. Chem. 88: 4451-4459, 1984). For gaseous ionization using electrospray plume, theoretical analysis also shows that \"super-atmospheric operation would be more preferable in space-charge-limited situations.\"(Int. J. Mass Spectrom. 300: 182-193, 2011). However, electrospray and the corona-based chemical ion source (APCI) in most commercial instrument are basically operated under an atmospheric pressure ambient, perhaps out of the concern of safety, convenience and simplicity in maintenance. Running the ion source at pressure much higher than 1 atm is not so common, but had been done by a number of groups as well as in our laboratory. A brief review on these ion sources will be given in this paper.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0041"},"PeriodicalIF":0.0,"publicationDate":"2015-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86748617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers. 揭示细胞周期中组蛋白H4修饰动力学的中下和化学蛋白质组学方法:组蛋白尾部肽修饰的无标记半定量，包括磷酸化和使用光反应交联剂高度敏感地捕获组蛋白PTM结合蛋白。

Mass spectrometry Pub Date : 2015-07-02 DOI: 10.5702/massspectrometry.A0039

Kazuki Yamamoto, Yoko Chikaoka, Gosuke Hayashi, Ryosuke Sakamoto, Ryuji Yamamoto, A. Sugiyama, T. Kodama, A. Okamoto, T. Kawamura

{"title":"Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers.","authors":"Kazuki Yamamoto, Yoko Chikaoka, Gosuke Hayashi, Ryosuke Sakamoto, Ryuji Yamamoto, A. Sugiyama, T. Kodama, A. Okamoto, T. Kawamura","doi":"10.5702/massspectrometry.A0039","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0039","url":null,"abstract":"Mass spectrometric proteomics is an effective approach for identifying and quantifying histone post-translational modifications (PTMs) and their binding proteins, especially in the cases of methylation and acetylation. However, another vital PTM, phosphorylation, tends to be poorly quantified because it is easily lost and inefficiently ionized. In addition, PTM binding proteins for phosphorylation are sometimes resistant to identification because of their variable binding affinities. Here, we present our efforts to improve the sensitivity of detection of histone H4 tail peptide phosphorylated at serine 1 (H4S1ph) and our successful identification of an H4S1ph binder candidate by means of a chemical proteomics approach. Our nanoLC-MS/MS system permitted semi-quantitative label-free analysis of histone H4 PTM dynamics of cell cycle-synchronized HeLa S3 cells, including phosphorylation, methylation, and acetylation. We show that H4S1ph abundance on nascent histone H4 unmethylated at lysine 20 (H4K20me0) peaks from late S-phase to M-phase. We also attempted to characterize effects of phosphorylation at H4S1 on protein-protein interactions. Specially synthesized photoaffinity bait peptides specifically captured 14-3-3 proteins as novel H4S1ph binding partners, whose interaction was otherwise undetectable by conventional peptide pull-down experiments. This is the first report that analyzes dynamics of PTM pattern on the whole histone H4 tail during cell cycle and enables the identification of PTM binders with low affinities using high-resolution mass spectrometry and photo-affinity bait peptides.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"44 1","pages":"A0039"},"PeriodicalIF":0.0,"publicationDate":"2015-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87754828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Probe Heating Method for the Analysis of Solid Samples Using a Portable Mass Spectrometer. 便携式质谱仪分析固体样品的探针加热方法。

Mass spectrometry Pub Date : 2015-04-20 DOI: 10.5702/massspectrometry.A0038

S. Kumano, Masuyuki Sugiyama, Masuyoshi Yamada, Kazushige Nishimura, H. Hasegawa, H. Morokuma, H. Inoue, Yuichiro Hashimoto

{"title":"Probe Heating Method for the Analysis of Solid Samples Using a Portable Mass Spectrometer.","authors":"S. Kumano, Masuyuki Sugiyama, Masuyoshi Yamada, Kazushige Nishimura, H. Hasegawa, H. Morokuma, H. Inoue, Yuichiro Hashimoto","doi":"10.5702/massspectrometry.A0038","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0038","url":null,"abstract":"We previously reported on the development of a portable mass spectrometer for the onsite screening of illicit drugs, but our previous sampling system could only be used for liquid samples. In this study, we report on an attempt to develop a probe heating method that also permits solid samples to be analyzed using a portable mass spectrometer. An aluminum rod is used as the sampling probe. The powdered sample is affixed to the sampling probe or a droplet of sample solution is placed on the tip of the probe and dried. The probe is then placed on a heater to vaporize the sample. The vapor is then introduced into the portable mass spectrometer and analyzed. With the heater temperature set to 130°C, the developed system detected 1 ng of methamphetamine, 1 ng of amphetamine, 3 ng of 3,4-methylenedioxymethamphetamine, 1 ng of 3,4-methylenedioxyamphetamine, and 0.3 ng of cocaine. Even from mixtures consisting of clove powder and methamphetamine powder, methamphetamine ions were detected by tandem mass spectrometry. The developed probe heating method provides a simple method for the analysis of solid samples. A portable mass spectrometer incorporating this method would thus be useful for the onsite screening of illicit drugs.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"2 11-12","pages":"A0038"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72494266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

MALDI Matrix Research for Biopolymers. 生物聚合物MALDI基质研究。

Mass spectrometry Pub Date : 2015-04-11 DOI: 10.5702/massspectrometry.A0037

Y. Fukuyama

引用次数: 13

Characterization of Mycolic Acids in Total Fatty Acid Methyl Ester Fractions from Mycobacterium Species by High Resolution MALDI-TOFMS. 高分辨率MALDI-TOFMS测定分枝杆菌总脂肪酸甲酯组分中霉菌酸。

Mass spectrometry Pub Date : 2015-04-01 DOI: 10.5702/massspectrometry.A0035

K. Teramoto, M. Suga, Takafumi Sato, T. Wada, Atsushi Yamamoto, N. Fujiwara

{"title":"Characterization of Mycolic Acids in Total Fatty Acid Methyl Ester Fractions from Mycobacterium Species by High Resolution MALDI-TOFMS.","authors":"K. Teramoto, M. Suga, Takafumi Sato, T. Wada, Atsushi Yamamoto, N. Fujiwara","doi":"10.5702/massspectrometry.A0035","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0035","url":null,"abstract":"Mycolic acids (MAs) are characteristic components of bacteria in the suborder Corynebacterineae, such as Mycobacterium. MAs are categorized into subclasses based on their functional bases (cyclopropane ring, methoxy, keto, and epoxy group). Since MAs have heterogeneity among bacterial species, analyzing of MAs are required in the chemotaxonomic field. However, their structural analysis is not easy because of their long carbon-chain lengths and several functional groups. In this study, total fatty acid (FA) methyl ester (ME) fraction of M. tuberculosis H37Rv was analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOFMS) with a spiral ion trajectory (MALDI spiral-TOFMS). The distributions of carbon-chain length and their relative peak intensities were confirmed with those obtained by analysis of each subclass fraction which was separated from total FA ME fraction using thin-layer chromatography (TLC). The observed major peaks were reliably assigned as MAs owing to the high mass accuracy (error<3 ppm). The types of MA subclasses, their distributions of carbon-chain lengths, their relative peak intensities, and the ratio of even- and odd-numbered carbon-chain MAs for the total FA ME fraction were consistent with those of MA subclass fractions. To visualize whole MAs, contour maps of relative peak intensities for whole MAs were created. The contour maps indicated the MA subclasses and their distributions of carbon-chains with relative peak intensities at a glance. Our proposed method allows simple characterization in a short time and thus enables the analysis of large numbers of samples, and it would contribute to the chemotaxonomy.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"133 1","pages":"A0035"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80011947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Method for the Compound Annotation of Conjugates in Nontargeted Metabolomics Using Accurate Mass Spectrometry, Multistage Product Ion Spectra and Compound Database Searching. 基于精确质谱、多级产物离子谱和化合物数据库检索的非靶向代谢组学共轭物化合物注释方法

Mass spectrometry Pub Date : 2015-04-01 DOI: 10.5702/massspectrometry.A0036

Tairo Ogura, T. Bamba, A. Tai, E. Fukusaki

{"title":"Method for the Compound Annotation of Conjugates in Nontargeted Metabolomics Using Accurate Mass Spectrometry, Multistage Product Ion Spectra and Compound Database Searching.","authors":"Tairo Ogura, T. Bamba, A. Tai, E. Fukusaki","doi":"10.5702/massspectrometry.A0036","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0036","url":null,"abstract":"Owing to biotransformation, xenobiotics are often found in conjugated form in biological samples such as urine and plasma. Liquid chromatography coupled with accurate mass spectrometry with multistage collision-induced dissociation provides spectral information concerning these metabolites in complex materials. Unfortunately, compound databases typically do not contain a sufficient number of records for such conjugates. We report here on the development of a novel protocol, referred to as ChemProphet, to annotate compounds, including conjugates, using compound databases such as PubChem and ChemSpider. The annotation of conjugates involves three steps: 1. Recognition of the type and number of conjugates in the sample; 2. Compound search and annotation of the deconjugated form; and 3. In silico evaluation of the candidate conjugate. ChemProphet assigns a spectrum to each candidate by automatically exploring the substructures corresponding to the observed product ion spectrum. When finished, it annotates the candidates assigning a rank for each candidate based on the calculated score that ranks its relative likelihood. We assessed our protocol by annotating a benchmark dataset by including the product ion spectra for 102 compounds, annotating the commercially available standard for quercetin 3-glucuronide, and by conducting a model experiment using urine from mice that had been administered a green tea extract. The results show that by using the ChemProphet approach, it is possible to annotate not only the deconjugated molecules but also the conjugated molecules using an automatic interpretation method based on deconjugation that involves multistage collision-induced dissociation and in silico calculated conjugation.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"426 1","pages":"A0036"},"PeriodicalIF":0.0,"publicationDate":"2015-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75043273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Application of Tapping-Mode Scanning Probe Electrospray Ionization to Mass Spectrometry Imaging of Additives in Polymer Films. 轻敲模式扫描探针电喷雾电离在聚合物薄膜添加剂质谱成像中的应用。

Mass spectrometry Pub Date : 2015-03-13 DOI: 10.5702/massspectrometry.S0050

Ryo Shimazu, Yoshinari Yamoto, Tomoya Kosaka, H. Kawasaki, R. Arakawa

引用次数: 3