Mass spectrometry最新文献_第10页

Visualization of Sampling and Ionization Processes in Scanning Probe Electrospray Ionization Mass Spectrometry. 扫描探针电喷雾电离质谱法中采样和电离过程的可视化。

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2019-03-07 DOI: 10.5702/massspectrometry.S0078

Bui Kamihoriuchi, Yoichi Otsuka, Aya Takeuchi, Futoshi Iwata, Takuya Matsumoto

{"title":"Visualization of Sampling and Ionization Processes in Scanning Probe Electrospray Ionization Mass Spectrometry.","authors":"Bui Kamihoriuchi, Yoichi Otsuka, Aya Takeuchi, Futoshi Iwata, Takuya Matsumoto","doi":"10.5702/massspectrometry.S0078","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0078","url":null,"abstract":"Ambient sampling and ionization techniques based on direct liquid extraction and electrospray ionization are of great value for rapid analysis and mass spectrometry imaging. Scanning probe electrospray ionization (SPESI) enables the sampling and ionization of analyte molecules in a solid material using a liquid bridge and electrospray, respectively, from a single capillary probe. To further improve SPESI, it is essential to understand the dynamic behavior of nanoliter volumes of liquids during sampling and ionization. In this study, the dynamic formation and breakage of the liquid bridge and the subsequent electrospray ionization were investigated by measuring the displacement of the capillary probe using a new optical technique. Measurements revealed that both the time from the formation of the liquid bridge to its breakage and the time from the breakage of the liquid bridge to the detection of analyte ions were correlated with the physical properties of the solvent. It was also found that both of these times were positively correlated with the flow rate. These results will not only lead to the improvement of sampling and ionization efficiencies but also afford a greater understanding of the physicochemical properties of charged nanoliter volumes of liquids.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"7 2","pages":"S0078"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.S0078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37459829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mass Spectrometry-Inspired Degradation of Disinfection By-Product, 2,6-Dichloro-1,4-benzoquinone, in Drinking Water by Heating. 饮用水中消毒副产物2,6-二氯-1,4-苯醌的加热质谱降解

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2018-06-29 DOI: 10.5702/massspectrometry.A0068

Jiying Pei, Ruiling Zhang, Chengchih Hsu, Yinghui Wang

引用次数: 3

Development of a Vacuum Electrospray Droplet Ion Gun for Secondary Ion Mass Spectrometry. 二次离子质谱分析用真空电喷雾离子枪的研制。

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2018-07-31 DOI: 10.5702/massspectrometry.A0069

Satoshi Ninomiya, Yuji Sakai, Lee Chuin Chen, Kenzo Hiraoka

引用次数: 3

Generation and Propagation of MALDI Ion Packets Probed by Sheet-Like Nanosecond UV Laser Light. 片状纳秒紫外激光探测MALDI离子包的产生和传播。

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2018-12-26 DOI: 10.5702/massspectrometry.A0071

Tatsuro Shirota, Kennosuke Hoshina

{"title":"Generation and Propagation of MALDI Ion Packets Probed by Sheet-Like Nanosecond UV Laser Light.","authors":"Tatsuro Shirota, Kennosuke Hoshina","doi":"10.5702/massspectrometry.A0071","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0071","url":null,"abstract":"A sheet-like ultraviolet (UV) probe laser is used to investigate the ejection and propagation of ion packets of matrix CHCA, which are produced by matrix-assisted laser desorption and ionization (MALDI). Laser irradiation of the expanding MALDI plume induced photodissociation of the CHCA-related ions, which existed in a sheet-like volume, leading to their absence in their MALDI signal profiles. The MALDI spectra were measured under varying conditions: the temporal delay of the lasers and the distance of the sheet-like probe laser from the MALDI sample surface. It was found that the center of the (CHCA)H+ packets were ejected at 46±11 ns after MALDI laser irradiation, while the (CHCA)2H+ packets were ejected at 64±12 ns, regardless of the magnitude of acceleration static high-voltage in 3.5-5.5 kV. This suggests that (CHCA)2H+ is formed by a proton transfer reaction from (CHCA)H+ to (CHCA)2 in the heated condensed phase and/or near the surface. This study represents the first experimental determination of ion ejection time in the MALDI process, which is also applicable to other species in the MALDI plume.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"7 1","pages":"A0071"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36806469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

OH-Radical Oxidation of Lung Surfactant Protein B on Aqueous Surfaces. 氧自由基氧化肺表面活性剂蛋白B在水表面。

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2018-11-21 DOI: 10.5702/massspectrometry.S0077

Shinichi Enami, Agustín J Colussi

{"title":"OH-Radical Oxidation of Lung Surfactant Protein B on Aqueous Surfaces.","authors":"Shinichi Enami, Agustín J Colussi","doi":"10.5702/massspectrometry.S0077","DOIUrl":"https://doi.org/10.5702/massspectrometry.S0077","url":null,"abstract":"Air pollutants generate reactive oxygen species on lung surfaces. Here we report how hydroxyl radicals (·OH) injected on the surface of water react with SP-B1-25, a 25-residue polypeptide surrogate of human lung surfactant protein B. Our experiments consist of intersecting microjets of aqueous SP-B1-25 solutions with O3/O2/H2O/N2(g) gas streams that are photolyzed into ·OH(g) in situ by 266 nm laser nanosecond pulses. Surface-sensitive mass spectrometry enables us to monitor the prompt (<10 μs) and simultaneous formation of primary O n -containing products/intermediates (n≤5) triggered by the reaction of ·OH with interfacial SP-B1-25. We found that O-atoms from both O3 and ·OH are incorporated into the reactive cysteine Cys8 and Cys11 and tryptophan Trp9 components of the hydrophobic N-terminus of SP-B1-25 that lies at the topmost layers of the air-liquid interface. Remarkably, these processes are initiated by ·OH additions rather than by H-atom abstractions from S-H, C-H, or N-H groups. By increasing the hydrophilicity of the N-terminus region of SP-B1-25, these transformations will impair its role as a surfactant.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"7 2","pages":"S0077"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.S0077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36812394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Physicochemical Prediction of Metabolite Fragmentation in Tandem Mass Spectrometry. 串联质谱法对代谢物碎片化的理化预测。

Mass spectrometry Pub Date : 2018-01-01 Epub Date: 2018-06-14 DOI: 10.5702/massspectrometry.A0066

Wataru Tanaka, Masanori Arita

{"title":"Physicochemical Prediction of Metabolite Fragmentation in Tandem Mass Spectrometry.","authors":"Wataru Tanaka, Masanori Arita","doi":"10.5702/massspectrometry.A0066","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0066","url":null,"abstract":"Current bottleneck of comprehensive non-target metabolite identification is insufficient spectral library. Many research groups have tried to build a theoretical product ion spectral library independent of measurement condition or settings, but mechanisms of metabolite fragmentation are not fully clarified. To achieve the mechanistic prediction of metabolite fragmentation which covers a wide range of metabolites, we will discuss utilization of physicochemical calculation. We introduce bonding patterns, which include two bound atoms and chemical groups adjacent to the bond. Cleavage of each bonding pattern is simulated and its activation energy is precisely calculated with quantum chemistry and assigned on metabolites. By tracing low-energy bond cleavages, fragmentation of a dipeptide molecule is successfully predicted. Prediction on another metabolite requires some additional features to fully reproduce its experimentally observed product ions. Physicochemical calculation shows its promising ability to predict fragmentation pathways only from metabolite structures, while required improvements suggested by comparison between our prediction and standard spectra stored in database are also discussed. Moreover, to construct a prediction strategy which withstands the vast metabolite space, we have to build a comprehensive list of bonding patterns and their activation energy. As theoretically possible bonding patterns are huge in number, proper simplification of the patterns must be implemented. We will discuss how to achieve it in addition to the prediction results.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"7 1","pages":"A0066"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36238604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass Spectrometry for the Detection of Endogenous Steroids and Steroid Abuse in (Race) Horses and Human Athletes 质谱法检测赛马和人类运动员内源性类固醇和类固醇滥用

Mass spectrometry Pub Date : 2017-06-07 DOI: 10.5772/INTECHOPEN.68593

Decloedt Anneleen, V. Anita, Vanhaecke Lynn

{"title":"Mass Spectrometry for the Detection of Endogenous Steroids and Steroid Abuse in (Race) Horses and Human Athletes","authors":"Decloedt Anneleen, V. Anita, Vanhaecke Lynn","doi":"10.5772/INTECHOPEN.68593","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.68593","url":null,"abstract":"The higher the pressure to win, the more athletes are inclined to take steps to improve one’s performance through questionable means. To minimize this, strict anti‐doping and medication rules are being enforced. All human and equine athletes are regularly subjected to doping analysis to prevent abuse of forbidden substances from affecting their performance. Anabolic‐androgenic steroids (AASs) have been part of the forbidden substances list for years, because of their muscle building and performance‐enhancing capacities and possible side effects. For most of the AAS, zero‐tolerance is held. However, some AASs can be endogenous to the athletes, such as for example testosterone in males. These endogenous steroids can render it very difficult to reveal steroid abuse. Specific mass spectrometric (MS) methods, including ultra‐high performance liquid chromatography‐MS (UHPLC‐MS/MS), high resolution mass spectrometry (HRMS) and gas chromatography–combustion‐isotope ratio MS (GC‐C‐IRMS), have been put forward to overcome these analytical difficulties. Currently, high‐tech metabolomic methods are being used to build athlete specific biological passports. In the near future, these passports might allow putting a stop to abuse, by staying ahead of the cheats. These are bright prospects, leading towards clean and fair sports competitions worldwide.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"49 1","pages":"229-251"},"PeriodicalIF":0.0,"publicationDate":"2017-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89998264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams 电喷雾滴离子束二次离子质谱分析肾癌

Mass spectrometry Pub Date : 2017-01-27 DOI: 10.5702/massspectrometry.A0053

S. Ninomiya, K. Yoshimura, L. Chen, S. Takeda, K. Hiraoka

{"title":"Secondary Ion Mass Spectrometry Analysis of Renal Cell Carcinoma with Electrospray Droplet Ion Beams","authors":"S. Ninomiya, K. Yoshimura, L. Chen, S. Takeda, K. Hiraoka","doi":"10.5702/massspectrometry.A0053","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0053","url":null,"abstract":"Tissue samples from renal cell carcinoma patients were analyzed by electrospray droplet ion beam-induced secondary ion mass spectrometry (EDI/SIMS). Positively- and negatively-charged secondary ions were measured for the cancerous and noncancerous regions of the tissue samples. Although specific cancerous species could not be found in both the positive and negative secondary ion spectra, the spectra of the cancerous and noncancerous tissues presented different trends. For instance, in the m/z range of 500–800 of the positive secondary ion spectra for the cancerous tissues, the intensities for several m/z values were lower than those of the m/z+2 peaks (indicating one double bond loss for the species), whereas, for the noncancerous tissues, the inverse trend was obtained. The tandem mass spectrometry (MS/MS) was also performed on the tissue samples using probe electrospray ionization (PESI), and some molecular ions produced by PESI were found to be fragmented into the ions observed in EDI/SIMS analysis. When the positive secondary ion spectra produced by EDI/SIMS were analyzed by principal component analysis, the results for cancerous and noncancerous tissues were separated. The EDI/SIMS method can be applied to distinguish between a cancerous and a noncancerous area with high probability.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88970120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

High Spatial Resolution Laser Desorption/Ionization Mass Spectrometry Imaging of Organic Layers in an Organic Light-Emitting Diode. 有机发光二极管中有机层的高空间分辨率激光解吸/电离质谱成像。

Mass spectrometry Pub Date : 2017-01-11 DOI: 10.5702/massspectrometry.A0052

Y. Tachibana, Y. Nakajima, T. Isemura, Kiyoshi Yamamoto, Takaya Satoh, J. Aoki, M. Toyoda

{"title":"High Spatial Resolution Laser Desorption/Ionization Mass Spectrometry Imaging of Organic Layers in an Organic Light-Emitting Diode.","authors":"Y. Tachibana, Y. Nakajima, T. Isemura, Kiyoshi Yamamoto, Takaya Satoh, J. Aoki, M. Toyoda","doi":"10.5702/massspectrometry.A0052","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0052","url":null,"abstract":"To improve the durability of organic materials in electronic devices, an analytical method that can obtain information about the molecular structure directly from specific areas on a device is desired. For this purpose, laser desorption/ionization mass spectrometry imaging (LDI-MSI) is one of the most promising methods. The high spatial resolution stigmatic LDI-MSI with MULTUM-IMG2 in the direct analysis of organic light-emitting diodes was shown to obtain a detailed mass image of organic material in the degraded area after air exposure. The mass image was observed to have a noticeably improved spatial resolution over typical X-ray photoelectron spectroscopy, generally used technique in analysis of electronic devices. A prospective m/z was successfully deduced from the high spatial resolution MSI data. Additionally, mass resolution and accuracy using a spiral-orbit TOF mass spectrometer, SpiralTOF, were also investigated. The monoisotopic mass for the main component, N,N'-di-1-naphthalenyl-N,N'-diphenyl-1,1'-biphenyl-4,4'-diamine (m/z 588), was measured with a mass resolution of approximately 80,000 and a mass error of about 5 mDa using an external calibrant. This high mass resolution and accuracy data successfully deduced a possible elemental composition of partially remained material in the degraded area, C36H24, which was determined as anthracene, 9-[1,1'-biphenyl]-4-yl-10-(2-naphthalenyl) by combining structural information with high-energy CID data. The high spatial resolution of 1 μm in LDI-MSI along with high mass resolution and accuracy could be useful in obtaining molecular structure information directly from specific areas on a device, and is expected to contribute to the evolution of electrical device durability.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"3 1","pages":"A0052"},"PeriodicalIF":0.0,"publicationDate":"2017-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75908364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Special Issue for The International Biological Mass Spectrometry (BMS) Symposium 2016 in Tokyo. 2016年东京国际生物质谱(BMS)研讨会特刊。

Mass spectrometry Pub Date : 2017-01-01 Epub Date: 2017-09-15 DOI: 10.5702/massspectrometry.K0011

Tohru Yamagaki

引用次数: 0