Mass spectrometry最新文献_第7页

Mass Spectrometry-Based Discovery of in vitro Kinome Substrates. 基于质谱法的体外Kinome底物发现。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-03-28 DOI: 10.5702/massspectrometry.A0082

Naoyuki Sugiyama

引用次数: 3

An Analytical System for Single-Cell Metabolomics of Typical Mammalian Cells Based on Highly Sensitive Nano-Liquid Chromatography Tandem Mass Spectrometry. 基于高灵敏度纳米液相色谱串联质谱的典型哺乳动物细胞单细胞代谢组学分析系统。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-03-17 DOI: 10.5702/massspectrometry.A0080

Kohta Nakatani, Yoshihiro Izumi, Kosuke Hata, Takeshi Bamba

{"title":"An Analytical System for Single-Cell Metabolomics of Typical Mammalian Cells Based on Highly Sensitive Nano-Liquid Chromatography Tandem Mass Spectrometry.","authors":"Kohta Nakatani, Yoshihiro Izumi, Kosuke Hata, Takeshi Bamba","doi":"10.5702/massspectrometry.A0080","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0080","url":null,"abstract":"The rapid development of next-generation sequencing techniques has enabled single-cell genomic and transcriptomic analyses, which have revealed the importance of heterogeneity in biological systems. However, analytical methods to accurately identify and quantify comprehensive metabolites from single mammalian cells with a typical diameter of 10-20 μm are still in the process of development. The aim of this study was to develop a single-cell metabolomic analytical system based on highly sensitive nano-liquid chromatography tandem mass spectrometry (nano-LC-MS/MS) with multiple reaction monitoring. A packed nano-LC column (3-μm particle-size pentafluorophenylpropyl Discovery HSF5 of dimensions 100 μm i.d.×180 mm) was prepared using a slurry technique. The optimized nano-LC-MS/MS method showed 3-132-fold (average value, 26-fold) greater sensitivity than semimicro-LC-MS/MS, and the detection limits for several hydrophilic metabolites, including amino acids and nucleic acid related metabolites were in the sub-fmol range. By combining live single-cell sampling and nano-LC-MS/MS, we successfully detected 18 relatively abundant hydrophilic metabolites (16 amino acids and 2 nucleic acid related metabolites) from single HeLa cells (n=22). Based on single-cell metabolic profiles, the 22 HeLa cells were classified into three distinct subclasses, suggesting differences in metabolic function in cultured HeLa cell populations. Our single-cell metabolomic analytical system represents a potentially useful tool for in-depth studies focused on cell metabolism and heterogeneity.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0080"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38057916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Mass Spectrometric Characterization of Histone H3 Isolated from in-Vitro Reconstituted and Acetylated Nucleosome Core Particle. 体外重组和乙酰化核小体核心颗粒中分离的组蛋白H3的质谱表征。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-10-31 DOI: 10.5702/massspectrometry.A0090

Kazumi Saikusa, Haruna Hidaka, Shunsuke Izumi, Satoko Akashi

{"title":"Mass Spectrometric Characterization of Histone H3 Isolated from in-Vitro Reconstituted and Acetylated Nucleosome Core Particle.","authors":"Kazumi Saikusa, Haruna Hidaka, Shunsuke Izumi, Satoko Akashi","doi":"10.5702/massspectrometry.A0090","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0090","url":null,"abstract":"Post-translational modifications (PTMs) of histone N-terminal tails in nucleosome core particle (NCP), such as acetylation, play crucial roles in regulating gene expression. To unveil the regulation mechanism, atomic-level structural analysis of in-vitro modified NCP is effective with verifying the PTMs of histones. So far, identification of PTMs of NCP originating from living cells has mainly been performed using mass spectrometry (MS) techniques, such as bottom-up approach. The bottom-up approach is the most established method for protein characterization, but it does not always provide sufficient information on the acetylated sites of lysine residues in the histone tails if trypsin digestion is carried out. For histone proteins, which have many basic amino acids, trypsin generates too many short fragments that cannot be perfectly analyzed by tandem MS. In this study, we investigated the in vitro acetylation sites in the histone H3 tail using a top-down sequence analysis, matrix-assisted laser desorption/ionization in-source decay (MALDI-ISD) experiment, in combination with aminopeptidase digestion. Aminopeptidase can cleave peptide bonds one-by-one from the N-terminus of peptides or proteins, generating N-terminally truncated peptides and/or proteins. As a result, it was identified that this method enables sequence characterization of the entire region of the H3 tail. Also, application of this method to H3 in in-vitro acetylated NCP enabled assigning acetylation sites of H3. Thus, this method was found to be effective for obtaining information on in-vitro acetylation of NCP for structural biology study.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0090"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38736753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS. 固相萃取-柱切换LC-MS/MS分析人粪便中血清素。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-03-31 DOI: 10.5702/massspectrometry.A0081

Yukiko Hirabayashi, Kiminori Nakamura, Tsuyoshi Sonehara, Daisuke Suzuki, Satoru Hanzawa, Yu Shimizu, Tomoyasu Aizawa, Koshi Nakamura, Akiko Tamakoshi, Tokiyoshi Ayabe

{"title":"Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS.","authors":"Yukiko Hirabayashi, Kiminori Nakamura, Tsuyoshi Sonehara, Daisuke Suzuki, Satoru Hanzawa, Yu Shimizu, Tomoyasu Aizawa, Koshi Nakamura, Akiko Tamakoshi, Tokiyoshi Ayabe","doi":"10.5702/massspectrometry.A0081","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0081","url":null,"abstract":"Serotonin, an important neurotransmitter, is produced mainly in intestines, and serotonin levels in feces can be an indicator of the intestinal environment. Human feces, however, contain a large amount of contaminants, which vary widely owing to food contents and the intestinal environment, and these contaminants would be expected to interfere with the determination of serotonin levels in human feces. To remove these contaminants and determine serotonin levels, we developed a new method using solid phase extraction (SPE) and column-switching LC-MS/MS. Serotonin, labeled with a stable isotope, was added to human feces samples prior to SPE as an internal standard to correct for individual differences in matrix effects. The recovery rate for SPE was 55.9-81.0% (intraday) and 56.5-78.1% (interday) for feces from two subjects. We analyzed 220 fecal samples from 96 subjects including 76 pregnant and post-delivery women. The endogenous serotonin content per unit weight of dried feces was 0.09-14.13 ng/mg for pregnant and post-delivery women and 0.30-9.93 ng/mg for the remaining subjects.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0081"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38057399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Quantitative Visualization of Lanthanum Accumulation in Lanthanum Carbonate-Administered Human Stomach Tissues Using Mass Spectrometry Imaging. 使用质谱成像技术定量可视化碳酸镧在人胃组织中的积累。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-07-13 DOI: 10.5702/massspectrometry.A0086

Shuichi Shimma, Yoshiki Makino, Kazuto Kojima, Takafumi Hirata

{"title":"Quantitative Visualization of Lanthanum Accumulation in Lanthanum Carbonate-Administered Human Stomach Tissues Using Mass Spectrometry Imaging.","authors":"Shuichi Shimma, Yoshiki Makino, Kazuto Kojima, Takafumi Hirata","doi":"10.5702/massspectrometry.A0086","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0086","url":null,"abstract":"Platinum, a transition metal that is widely used in anti-cancer agents, also results in the development of nephropathy due to severe adverse reactions caused by platinum-induced nephrotoxicity. Reports on imaging with metals other than platinum remain are limited, even in preclinical studies. Furthermore, most of these are case reports, and the relationship between the distribution of the metal and clinical observations in human samples is not well understood. Here we report on visualizing lanthanum (139La), a component of Fosrenol, which is usually used for the treatment of hyperphosphatemia. Gastric inflammation, also known as hemorrhagic gastritis, is the main adverse event caused by Fosrenol. To conduct this study, 139La was visualized in gastric biopsy samples obtained from a patient using quantitative laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). We also compared the distribution of 139La in tissue and histochemical results. The areas where 139La accumulated corresponded to the macrophage-positive areas observed in immunohistochemistry studies using an anti-CD68 antibody. In contrast, we observed a debris-like crystal morphology in hematoxylin and eosin staining tissues. The debris was also associated with 139La accumulation. The abnormal accumulation of 139La crystals caused the observed inflammation. This phenomenon was previously characterized, but this is the first report in which 139La distribution and histochemical results are compared using LA-ICP-MS.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0086"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38238996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Diagnostic Glycopeptide Markers of Congenital Disorders of Glycosylation. 基质辅助激光解吸/电离质谱法检测先天性糖基化疾病的诊断糖肽标记物。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-04-23 DOI: 10.5702/massspectrometry.A0084

Yoshinao Wada

{"title":"Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry to Detect Diagnostic Glycopeptide Markers of Congenital Disorders of Glycosylation.","authors":"Yoshinao Wada","doi":"10.5702/massspectrometry.A0084","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0084","url":null,"abstract":"Congenital disorders of glycosylation (CDG), an increasingly recognized group of diseases that affect glycosylation, comprise the largest known subgroup of approximately 100 responsible genes related to N-glycosylation. This subgroup presents various molecular abnormalities, of either the CDG-I or the CDG-II type, attributable to a lack of glycans or abnormal glycoform profiles, respectively. The most effective approach to identifying these N-glycosylation disorders is mass spectrometry (MS) using either released glycans, intact glycoproteins or proteolytic peptides as analytes. Among these, MS of tryptic peptides derived from transferrin can be used to reliably identify signature peptides that are characteristic of CDG-I and II. In the present study, matrix-assisted laser desorption/ionization (MALDI) MS was applied to various N-glycosylation disorders including ALG1-CDG, B4GALT1-CDG, SLC35A2-CDG, ATP6V0A2-CDG, TRAPPC11-CDG and MAN1B1-CDG. This method does not require the prior enrichment of glycopeptides or chromatographic separation, and thus serves as a practical alternative to liquid chromatography-electrospray ionization MS. The signature peptides are biomarkers of CDG.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0084"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5702/massspectrometry.A0084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38057402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A Brief History of Mass Spectrometry 质谱简史

Mass spectrometry Pub Date : 2019-07-15 DOI: 10.1002/9781119377368.ch2

M. Smoluch, J. Silberring

引用次数: 2

Mass Spectrometry Applications 质谱应用

Mass spectrometry Pub Date : 2019-07-15 DOI: 10.1007/978-1-4614-5626-1_5

C. Soler, J. Rubert, J. Mañes

引用次数: 1

Mass Spectrometry Applications 质谱应用

Mass spectrometry Pub Date : 2019-06-24 DOI: 10.1002/9781119377368.ch8

V. Cunsolo, S. Foti, J. Ner‐Kluza, A. Drabik, J. Silberring, V. Muccilli, R. Saletti, Katarzyna Pawlak, E. Harwood, Fang Yu, P. Ciborowski, R. Anczkiewicz, Kathrin Altweg, G. Spoto, A. Pawlaczyk, M. Szynkowska, M. Smoluch, D. Kwiatkowska

引用次数: 0

Basic Definitions 基本的定义

Mass spectrometry Pub Date : 2019-06-24 DOI: 10.1002/9781119377368.ch3

M. Smoluch, Kinga Piechura

引用次数: 0