Mass spectrometry最新文献_第6页

A Method for Expanding Mass Range on a Multi-Turn Time-of-Flight Mass Spectrometer by a Lap Superimposed Spectrum. 一种用搭接叠加谱扩展多转飞行时间质谱仪质量范围的方法。

Mass spectrometry Pub Date : 2021-01-01 Epub Date: 2021-10-14 DOI: 10.5702/massspectrometry.A0098

Toshinobu Hondo, Michisato Toyoda

{"title":"A Method for Expanding Mass Range on a Multi-Turn Time-of-Flight Mass Spectrometer by a Lap Superimposed Spectrum.","authors":"Toshinobu Hondo, Michisato Toyoda","doi":"10.5702/massspectrometry.A0098","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0098","url":null,"abstract":"A time-of-flight mass spectrometer that uses a closed-orbit flight path can achieve a high mass resolving power and a high mass accuracy with a small instrument footprint. It has long been known that a drawback to a closed flight path is an obtained spectrum may contain peaks by ions at a different number of laps. A lower m/z ion may overtake higher m/z ions, resulting in the peak being superimposed on an acquired mass spectrum; therefore, such a mass bandwidth of the analyzer is limited to a narrow range given the current situation. However, recent research has documented a solution to the problem based on careful study of the equation of motion of an ion in a closed-path analyzer. All of the ions in the analyzer remain in motion in orbit by the nature of the closed flight path, thus resulting in a superimposed spectrum with the width of the orbital period of the highest mass in the sample matrix, which contains several different lap numbers. When target ions for the sample are known in advance, the time-of-flight for a given m/z can be determined regardless of the lap number under given analyzer conditions, and peak assignment can be self-validated by comparison to a mass spectrum acquired at a different lap condition. Furthermore, the m/z value for an unknown ion can also be determined by comparing time-of-flight values on spectra acquired at different lap conditions.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"10 1","pages":"A0098"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8514288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39563876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas-Phase Adsorption of N₂ on Protonated Molecules and Its Application to the Structural Elucidation of Small Molecules. 质子化分子对N2的气相吸附及其在小分子结构解析中的应用。

Mass spectrometry Pub Date : 2021-01-01 Epub Date: 2021-06-10 DOI: 10.5702/massspectrometry.A0096

Hiromori Murashima, Akimasa Fujihara

{"title":"Gas-Phase Adsorption of N2 on Protonated Molecules and Its Application to the Structural Elucidation of Small Molecules.","authors":"Hiromori Murashima, Akimasa Fujihara","doi":"10.5702/massspectrometry.A0096","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0096","url":null,"abstract":"The gas-phase adsorption of N2 on protonated serine (Ser, C3H7NO3), threonine (Thr, C4H9NO3), glycine (Gly, C2H5NO2), and 2-aminoethanol (C2H7NO) was investigated using a tandem mass spectrometer equipped with an electrospray ionization source and a cold ion trap. N2 molecules were adsorbed on the free X-H (X=O and N) groups of protonated molecules. Gas-phase N2 adsorption-mass spectrometry detected the presence of free X-H groups in the molecular structures, and was applied to the structural elucidation of small molecules. When the 93 structures with an elemental composition of C3H7NO3 were filtered using the gas-phase N2 adsorption-mass spectrometry results for Ser, the number of possible molecular structures was reduced to 8 via the quantification of the X-H groups. Restricting and minimizing the number of possible candidates were effective steps in the structural elucidation process. Gas-phase N2 adsorption-mass spectrometry combined with mass spectrometry-based techniques has the potential for being useful for elucidating the molecular structures of a variety of molecules.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"10 1","pages":"A0096"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39238233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Corona Discharge and Field Electron Emission in Ambient Air Using a Sharp Metal Needle: Formation and Reactivity of CO₃ ^-• and O₂ ^-•. 环境空气中使用锋利金属针的电晕放电和场电子发射:CO3 -•和O2 -•的形成和反应性。

Mass spectrometry Pub Date : 2021-01-01 Epub Date: 2021-12-25 DOI: 10.5702/massspectrometry.A0100

Kenzo Hiraoka, Stephanie Rankin-Turner, Satoshi Ninomiya, Haruo Shimada, Kazumasa Kinoshita, Shinichi Yamabe

{"title":"Corona Discharge and Field Electron Emission in Ambient Air Using a Sharp Metal Needle: Formation and Reactivity of CO3 -• and O2 -•.","authors":"Kenzo Hiraoka, Stephanie Rankin-Turner, Satoshi Ninomiya, Haruo Shimada, Kazumasa Kinoshita, Shinichi Yamabe","doi":"10.5702/massspectrometry.A0100","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0100","url":null,"abstract":"CO3 -• and O2 -• are known to be strong oxidizing reagents in biological systems. CO3 -• in particular can cause serious damage to DNA and proteins by H• abstraction reactions. However, H• abstraction of CO3 -• in the gas phase has not yet been reported. In this work we report on gas-phase ion/molecule reactions of CO3 -• and O2 -• with various molecules. CO3 -• was generated by the corona discharge of an O2 reagent gas using a cylindrical tube ion source. O2 -• was generated by the application of a 15 kHz high frequency voltage to a sharp needle in ambient air at the threshold voltage for the appearance of an ion signal. In the reactions of CO3 -•, a decrease in signal intensities of CO3 -• accompanied by the simultaneous increase of that of HCO3 - was observed when organic compounds with H-C bond energies lower than ∼100 kcal mol-1 such as n-hexane, cyclohexane, methanol, ethanol, 1-propanol, 2-propanol, and toluene were introduced into the ion source. This clearly indicates the occurrence of H• abstraction. O2 -• abstracts H+ from acid molecules such as formic, acetic, trifluoroacetic, nitric and amino acids. Gas-phase CO3 -• may play a role as a strong oxidizing reagent as it does in the condensed phase. The major discharge product CO3 -• in addition to O2 -•, O3, and NO x • that are formed in ambient air may cause damage to biological systems.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"10 1","pages":"A0100"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39792150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Phosphorylation on the Collision Cross Sections of Peptide Ions in Ion Mobility Spectrometry 离子迁移谱法中磷酸化对肽离子碰撞截面的影响

Mass spectrometry Pub Date : 2020-06-15 DOI: 10.1101/2020.06.15.151639

Kosuke Ogata, Chih-Hsiang Chang, Y. Ishihama

引用次数: 8

Temperature-Resolved Proton Transfer Reactions of Biomolecular Ions. 生物分子离子的温度分辨质子转移反应。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-03-31 DOI: 10.5702/massspectrometry.A0083

Shinji Nonose

引用次数: 0

Analytical Capability of High-Time Resolution-Multiple Collector-Inductively Coupled Plasma-Mass Spectrometry for the Elemental and Isotopic Analysis of Metal Nanoparticles. 高时间分辨率-多重收集器-电感耦合等离子体质谱法分析金属纳米颗粒元素和同位素的能力。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-06-12 DOI: 10.5702/massspectrometry.A0085

Takafumi Hirata, Shuji Yamashita, Mirai Ishida, Toshihiro Suzuki

{"title":"Analytical Capability of High-Time Resolution-Multiple Collector-Inductively Coupled Plasma-Mass Spectrometry for the Elemental and Isotopic Analysis of Metal Nanoparticles.","authors":"Takafumi Hirata, Shuji Yamashita, Mirai Ishida, Toshihiro Suzuki","doi":"10.5702/massspectrometry.A0085","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0085","url":null,"abstract":"We measured the Re/Os (185Re/188Os) and 187Os/188Os ratios from nanoparticles (NPs) using a multiple collector-inductively coupled plasma-mass spectrometer equipped with high-time resolution ion counters (HTR-MC-ICP-MS). Using the HTR-MC-ICP-MS system developed in this study, the simultaneous data acquisition of four isotopes was possible with a time resolution of up to 10 μs. This permits the quantitative analysis of four isotopes to be carried out from transient signals (e.g., <0.6 ms) emanating from the NPs. Iridium-Osmium NPs were produced from a naturally occurring Ir-Os alloy (ruthenosmiridium from Hokkaido, Japan; osmiridium from British Columbia, Canada; iridosmine from the Urals region of Russia) through a laser ablation technique, and the resulting nanoparticles were collected by bubbling water through a suspension. The 187Os/188Os ratios for individual NPs varied significantly, mainly due to the counting statistics of the 187Os and 188Os signals. Despite the large variation in the measured ratios, the resulting 187Os/188Os ratios for three Ir-Os bearing minerals, were 0.121±0.013 for Hokkaido, 0.110±0.012 for British Columbia, and 0.122±0.020 for the Urals, and these values were in agreement with the ratios obtained by the conventional laser ablation-MC-ICP-MS technique. The data obtained here provides a clear demonstration that the HTR-MC-ICP-MS technique can become a powerful tool for monitoring elemental and isotope ratios from NPs of multiple components.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0085"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38109445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Kendrick Analysis and Complex Isotopic Patterns: A Case Study of the Compositional Analysis of Pristine and Heated Polybrominated Flame Retardants by High-Resolution MALDI Mass Spectrometry. Kendrick分析和复杂同位素模式:用高分辨率MALDI质谱分析原始和加热多溴系阻燃剂的成分。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-02-06 DOI: 10.5702/massspectrometry.A0079

Sayaka Nakamura, Hiroaki Sato, Thierry N J Fouquet

{"title":"Kendrick Analysis and Complex Isotopic Patterns: A Case Study of the Compositional Analysis of Pristine and Heated Polybrominated Flame Retardants by High-Resolution MALDI Mass Spectrometry.","authors":"Sayaka Nakamura, Hiroaki Sato, Thierry N J Fouquet","doi":"10.5702/massspectrometry.A0079","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0079","url":null,"abstract":"The Kendrick analysis is used for the processing and visualization of mass spectra obtained from polymers containing C, H, O and/or Si with simple isotopic patterns (monoisotope=lightest isotope=most intense isotope for short chains). In the case of heteroatoms with complex isotopic patterns, the impact of the chosen isotope on point alignments in Kendrick plots has not been examined extensively. Rich isotopic patterns also make the evaluation of the mass and nature of the repeating unit and end-groups more difficult from the mass spectrum in the case of unknown samples due to the number of peaks and the absence of a monoisotopic peak. Using a polybrominated polycarbonate as running example, we report that horizontal point alignments can be obtained in a Kendrick plot using the mass of the most abundant isotope instead of the monoisotopic mass as is usually done. Rotating the plot (\"reverse Kendrick analysis\") helps to accurately evaluate the mass of the most abundant isotope of the repeating unit, as well as the nature of the brominated neutral expelled upon gentle heating (debromination or dehydrobromination). The whole procedure is then applied to the characterization of an unknown polybrominated flame retardant in an industrial formulation before and after heating.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0079"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37726939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Selective Extraction of a Monoisotopic Ion While Keeping the Other Ions in Flight on a Multi-Turn Time-of-Flight Mass Spectrometer. 在多转飞行时间质谱仪上选择提取单同位素离子同时保持其他离子飞行。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-08-20 DOI: 10.5702/massspectrometry.A0088

Toshinobu Hondo, Hiroshi Kobayashi, Michisato Toyoda

{"title":"Selective Extraction of a Monoisotopic Ion While Keeping the Other Ions in Flight on a Multi-Turn Time-of-Flight Mass Spectrometer.","authors":"Toshinobu Hondo, Hiroshi Kobayashi, Michisato Toyoda","doi":"10.5702/massspectrometry.A0088","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0088","url":null,"abstract":"Using a multi-turn time-of-flight (TOF) mass spectrometer, we have extracted a single xenon isotope ion, 129Xe+, from its orbit at given a lap number without disturbing the rest of isotopes. After detecting the 129Xe+ at 20 laps, the rest of the xenon isotope spectrum was obtained at 30 laps, which generated a TOF spectrum where the TOF difference between 129Xe+ and 130Xe+ was 87.4 μs while 130Xe+ and 131Xe+ were 1.03 μs. The time distance between 129Xe+ and other isotopes can be set by any lap difference that is a factor of 8.7 μs, which depends on the acceleration voltage and the mass of the ion. Method accuracy was verified by comparing the isotopic abundance ratio of the xenon sample after withdrawing one of the ions from the isotope cluster to the abundance ratio obtained from the conventional method. The TOF stability was also evaluated at various lap numbers between 10 to 230.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0088"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38392147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Enhancement of Ionization Efficiency Using Zeolite in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Multiple Drugs in Cancer Cells (Mass Spectrometry of Multiple Drugs in Cells Using Zeolite). 在基质辅助激光解吸/电离质谱法中使用沸石提高肿瘤细胞内多种药物的电离效率(细胞内多种药物使用沸石质谱法)。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-12-04 DOI: 10.5702/massspectrometry.A0091

Hiroki Kannen, Shusei Nomura, Hisanao Hazama, Yasufumi Kaneda, Tatsuya Fujino, Kunio Awazu

{"title":"Enhancement of Ionization Efficiency Using Zeolite in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Multiple Drugs in Cancer Cells (Mass Spectrometry of Multiple Drugs in Cells Using Zeolite).","authors":"Hiroki Kannen, Shusei Nomura, Hisanao Hazama, Yasufumi Kaneda, Tatsuya Fujino, Kunio Awazu","doi":"10.5702/massspectrometry.A0091","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0091","url":null,"abstract":"Combined therapy using photodynamic therapy (PDT) and chemotherapy has been proposed for anticancer-drug-resistant cancer cells. To evaluate the efficacy of such a combined therapy, the uptakes of an anticancer drug and a photosensitizer in cancer cells must be assessed. Mass spectrometry using matrix-assisted laser desorption/ionization can detect multiple drugs simultaneously. Human prostate cancer cells PC-3 or docetaxel-resistant cancer cells PC-3-DR were incubated in a serum-free medium containing a photosensitizer, protoporphyrin IX (PpIX), and an anticancer drug, docetaxel. A zeolite matrix was created by mixing 6-aza-2-thiothymine and NaY5.6 zeolite, and dissolving in water with 50% acetone. Ions were obtained with a time-of-flight mass spectrometer using a Nd:YAG laser at a wavelength of 355 nm. The cell morphology was preserved by washing the cells with ammonium acetate and drying in a vacuum after drug administration. Protonated PpIX (m/z 563.3) and the sodium adduct ion of docetaxel (m/z 829.9) were obtained from PC-3 cells simultaneously using the zeolite matrix. On the other hand, PpIX was detected but ions originating from docetaxel were not detected from PC-3-DR cells. The result indicated the efficacy of PDT for docetaxel-resistant cancer cells.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"9 1","pages":"A0091"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38693905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Metabolic Visualization Reveals the Distinct Distribution of Sugars and Amino Acids in Rice Koji. 代谢可视化揭示了米曲中糖和氨基酸的独特分布。

Mass spectrometry Pub Date : 2020-01-01 Epub Date: 2020-08-26 DOI: 10.5702/massspectrometry.A0089

Adinda Putri Wisman, Yoshihiro Tamada, Shuji Hirohata, Eiichiro Fukusaki, Shuichi Shimma

引用次数: 3