{"title":"排除生物分子LC-MS分析中的残留:神经肽Y的案例。","authors":"Tohru Yamagaki, Takashi Yamazaki","doi":"10.5702/massspectrometry.S0083","DOIUrl":null,"url":null,"abstract":"<p><p>We describe systematic troubleshooting of the carry-over of neuropeptide Y (NPY) in LC-MS analysis. The objective was to remove candidate parts of the LC-MS system that are responsible for carry over one-by-one. The findings indicate that the carry-over of NPY occurs on the column, particularly in the guard column and at the consumable seals of the sample-needle and high-pressure valves. The methodology demonstrates that it is possible to troubleshoot carry-over in an LC-MS system in a systematic and logical manner.</p>","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"8 2","pages":"S0083"},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709882/pdf/","citationCount":"4","resultStr":"{\"title\":\"Troubleshooting Carry-Over in the LC-MS Analysis of Biomolecules: The Case of Neuropeptide Y.\",\"authors\":\"Tohru Yamagaki, Takashi Yamazaki\",\"doi\":\"10.5702/massspectrometry.S0083\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We describe systematic troubleshooting of the carry-over of neuropeptide Y (NPY) in LC-MS analysis. The objective was to remove candidate parts of the LC-MS system that are responsible for carry over one-by-one. The findings indicate that the carry-over of NPY occurs on the column, particularly in the guard column and at the consumable seals of the sample-needle and high-pressure valves. The methodology demonstrates that it is possible to troubleshoot carry-over in an LC-MS system in a systematic and logical manner.</p>\",\"PeriodicalId\":18243,\"journal\":{\"name\":\"Mass spectrometry\",\"volume\":\"8 2\",\"pages\":\"S0083\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709882/pdf/\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mass spectrometry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5702/massspectrometry.S0083\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/2/26 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Physics and Astronomy\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mass spectrometry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5702/massspectrometry.S0083","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/2/26 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Physics and Astronomy","Score":null,"Total":0}
Troubleshooting Carry-Over in the LC-MS Analysis of Biomolecules: The Case of Neuropeptide Y.
We describe systematic troubleshooting of the carry-over of neuropeptide Y (NPY) in LC-MS analysis. The objective was to remove candidate parts of the LC-MS system that are responsible for carry over one-by-one. The findings indicate that the carry-over of NPY occurs on the column, particularly in the guard column and at the consumable seals of the sample-needle and high-pressure valves. The methodology demonstrates that it is possible to troubleshoot carry-over in an LC-MS system in a systematic and logical manner.
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