Lipids最新文献

{"title":"Dietary triacetin, but not medium chain triacylglycerides, blunts weight gain in diet-induced rat model of obesity","authors":"Giulia Cisbani,&nbsp;Raphaël Chouinard-Watkins,&nbsp;Mackenzie E. Smith,&nbsp;Arezou Malekanian,&nbsp;Rodrigo Valenzuela,&nbsp;Adam H. Metherel,&nbsp;Richard P. Bazinet","doi":"10.1002/lipd.12381","DOIUrl":"10.1002/lipd.12381","url":null,"abstract":"<p>Consumption of a Western diet (WD) is known to increase the risk of obesity. Short or medium chain fatty acids influence energy metabolism, and triacetin, a synthetic short chain triacylglyceride, has been shown to lower body fat under normal conditions. This study aimed to investigate if triacetin as part of a WD modifies rat weight and body fat. Male rats were fed a control diet or WD for 8 weeks. At week 8, rats in the WD group were maintained on a WD diet or switched to a WD diet containing 30% energy from medium-chain triacylglyceride (WD-MCT) or triacetin (WD-T) for another 8 weeks. At week 16, rats were euthanized and liver, adipose and blood were collected. Tissue fatty acids (FAs) were quantified by gas chromatography (GC) and hepatic FAs were measured by GC-combustion-isotope ratio mass spectrometry for δ¹³C-palmitic acid (PAM)—a novel marker of de novo lipogenesis (DNL). Rats fed WD-T had a body weight not statistically different to the control group, and gained less body weight than rats fed WD alone. Furthermore, WD-T fed rats had a lower fat mass, and lower total liver and plasma FAs compared to the WD group. Rats fed WD-T did not differ from WD in blood ketone or glucose levels, however, had a significantly lower hepatic δ¹³C-PAM value than WD fed rats; suggestive of lower DNL. In summary, we show that triacetin has the potential to blunt weight gain and adipose tissue accumulation in a rodent model of obesity, possibly due to a decrease in DNL.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 6","pages":"257-270"},"PeriodicalIF":1.9,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aocs.onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12381","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138299515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tax effects on foreign direct investment-Just a rerouting.","authors":"Dmitry Erokhin","doi":"10.1111/twec.13465","DOIUrl":"10.1111/twec.13465","url":null,"abstract":"<p><p>This paper investigates tax-related determinants of indirect foreign direct investment (FDI). In particular, it studies the effects of bilateral effective average tax rates, the strength of anti-tax avoidance rules in host countries and tax haven status of home countries on the volume of indirect FDI host countries receive. The paper uses the fourth edition of the OECD Benchmark Definition of Foreign Direct Investment (BMD4) database, which distinguishes between ultimate and immediate FDI. Methodologically, the paper relies on the standard gravity equation for FDI and applies the Poisson pseudo-maximum likelihood estimation model. The paper shows that ultimate FDI is not influenced by tax-related factors but only real economic determinants, whereas tax rates affect immediate FDI. This finding suggests that previous research may have overestimated the tax elasticity of FDI, and taxes do not have an impact on location decisions of FDI, but rather the route of investing-direct or indirect. The paper defines indirect FDI as the difference between ultimate and immediate FDI and finds that high bilateral effective average tax rates encourage indirect FDI. The finding is robust under different specifications.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"36 1","pages":"2808-2834"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10947096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74160209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"9-POHSA prevents NF-kB activation and ameliorates LPS-induced inflammation in rat hepatocytes","authors":"Jiro Hasegawa Situmorang,&nbsp;Ming-Cheng Chen,&nbsp;Wei-Wen Kuo,&nbsp;Shinn-Zong Lin,&nbsp;Cheng-Yen Shih,&nbsp;Pi-Yu Lin,&nbsp;Ching-Hui Loh,&nbsp;Chih-Yang Huang","doi":"10.1002/lipd.12380","DOIUrl":"10.1002/lipd.12380","url":null,"abstract":"<p>Liver inflammation has become increasingly prevalent in recent years, leading to the development of diseases like hepatitis, alcoholic liver disease, and fatty liver disease. One factor that has been linked to liver inflammation is increased levels of lipopolysaccharides (LPS), which can be caused by poor diets and sedentary lifestyles that contribute to liver inflammation. There is promising research on a new class of lipids called fatty acid esters of hydroxy fatty acids (FAHFAs), which have been shown to potentiate insulin release and exert an anti-inflammatory effect. Specifically, one type of FAHFA called 9-POHSA (palmitoleic acid ester of 9-hydroxy stearic acid) has been studied for its potential to attenuate inflammation-related indexes induced by LPS in hepatocytes, which play a critical role in the progression of liver inflammation. This study found that following LPS treatment, tumor necrosis factor- α, interleukin-6, and connective tissue growth factor (CTGF) were upregulated and increased cell migration, but 9-POHSA pre-treatment attenuated the upregulation of these markers and prevented cell migration induced by LPS. Using flowcytometry analysis, intracellular reactive oxygen species (ROS) was found to be responsible for CTGF upregulation. In addition, the effects of 9-POHSA were likely associated with its inhibition of the activation of the NF-kB. These results suggest that 9-POHSA has potential as a therapy for liver inflammation and fibrosis by attenuating inflammation-related indexes induced by LPS in hepatocytes. This study provides important insight into the mechanisms of liver inflammation and the potential for new treatments to address liver diseases.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"241-249"},"PeriodicalIF":1.9,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Consumption of synbiotic bread decreases triacylglycerol and VLDL levels while increasing HDL levels in serum from patients with type-2 diabetes”","authors":"","doi":"10.1002/lipd.12379","DOIUrl":"10.1002/lipd.12379","url":null,"abstract":"<p>Shakeri, H, Hadaegh, H, Abedi, F, Tajabadi-Ebrahimi, M, Mazroii, N, Ghandi, Y and Asemi, Z. Consumption of synbiotic bread decreases triacylglycerol and VLDL levels while increasing HDL levels in serum from patients with type-2 diabetes. Lipids. 2014;49:695–701. https://doi.org/10.1007/s11745-014-3901-z.</p><p>The journal is aware of a number of issues raised regarding the integrity of research published by Zatolleh Asemi and colleagues (Grey et al., <span>2020</span>). An investigation into these concerns has been conducted by The National Committee for Ethics in Biomedical Research Iran, in coordination with Kashan University of Medical Sciences (KAUMS). The Editor has independently considered the concerns reported for this article and did not find evidence of questionable research practices at this time. The following corrections have therefore been made.</p><p>In the above-referenced published article, the study was reported to have taken place between October 2013 and December 2013. This is an apparent error in the preparation of the manuscript. The correct timing of the study was between December 2013 and January 2014. In the published article Figure 1 and Tables 1, 2, and 3 state that the number of participants for which samples were analyzed was 26. This is a systematic typographical error; the correct number is 24 participants. The authors neglected to acknowledge the contribution to the study of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh. The correct version of the acknowledgment should therefore be: Acknowledgments: The present study was supported by a Grant (No. 92107) from the Vice-Chancellor for Research, KAUMS, Kashan, Iran. The authors would like to thank the staff of Gholabchi Clinic (Kashan, Iran) for their assistance in this project. We are grateful to the Research and Development Department of Sahar Bread Company, Tehran, Iran that provided probiotic and synbiotic products for the present study. Furthermore, we are grateful to the Research and Development Department of Tak Gene Zist Company, Tehran, Iran that provided Lactobacillus sporogenes for this study. The authors wish to acknowledge the contributions of Drs. Maryam Tajabadi-Ebrahimi and Haleh Hadaegh who conducted the analysis of the blood samples. The authors apologize for any inconvenience these errors and oversights may have caused.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"253"},"PeriodicalIF":1.9,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Synbiotic food consumption reduces levels of triacylglycerols and VLDL, but not cholesterol, LDL, or HDL in plasma from pregnant women”","authors":"","doi":"10.1002/lipd.12378","DOIUrl":"10.1002/lipd.12378","url":null,"abstract":"<p>Taghizadeh, M, Hashemi, T, Shakeri, H, Abedi, F, Sabihi, S-S, Alizadeh, S-A and Asemi, Z. Synbiotic food consumption reduces levels of triacylglycerols and VLDL, but not cholesterol, LDL, or HDL in plasma from pregnant women. Lipids. 2014;49:155–161. https://doi.org/10.1007/s11745-013-3867-2.</p><p>The journal is aware of a number of issues raised regarding the integrity of research published by Zatolleh Asemi and colleagues (Grey et al., <span>2020</span>). An investigation into these concerns has been conducted by The National Committee for Ethics in Biomedical Research Iran, in coordination with Kashan University of Medical Sciences (KAUMS). The Editor has independently considered the concerns reported for this article and finds no robust evidence of questionable research practices at this time. The following corrections have therefore been made.</p><p>It is stated on page 156 of the published article that: “Pregnant women, primigravida, aged 18 to 35-years-old at 27 weeks of gestation were recruited in this study.” It is also stated on the same page that: “To obtain detailed information about the dietary intakes of study participants, all women were entered into a 2-week run-in period; during which all subjects had to refrain from taking synbiotic or any other probiotic food. At the end of the run-in period (27 weeks of gestation), subjects were randomly assigned to consume 18 g/day of synbiotic or control food for 9 weeks.” The first statement indicates that participants entered the study at 27 weeks gestation, while the second statement indicates that the participants had completed the run-in period at 27 weeks, and hence would have been enrolled into the study at an earlier gestational age. The Principal Investigator (Zatolleh Asemi) has explained that the first statement is the correct account of the study protocol. The authors apologize for any inconvenience these errors and oversights may have caused.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"251"},"PeriodicalIF":1.9,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10194911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidants ameliorate oxidative stress in alcoholic liver injury by modulating lipid metabolism and phospholipid homeostasis","authors":"Xiaoxu Wang,&nbsp;Bin Liu,&nbsp;Yanjun Liu,&nbsp;Yuliu Wang,&nbsp;Zhigao Wang,&nbsp;Yu Song,&nbsp;Jie Xu,&nbsp;Changhu Xue","doi":"10.1002/lipd.12377","DOIUrl":"10.1002/lipd.12377","url":null,"abstract":"<p>Alcoholic liver disease (ALD) is a significant risk factor in the global disease burden. The antioxidants vitamin C (Vc) and <i>N</i>-acetyl cysteine (NAC) have shown hepatoprotective effects in preventing and treating ALD. However, the correlation between the improved effect of antioxidants and lipid metabolism is still unclear. In this study, AML12 cells and C57BL/6 mice stimulated with alcohol were used to investigate the protective effects and potential mechanisms of two antioxidants (Vc and NAC) on alcoholic liver injury. Results showed that Vc and NAC attenuated intracellular lipid accumulation and oxidative damage induced by excessive alcohol exposure in hepatic AML12 cells. The <i>in vivo</i> results indicated that antioxidants ameliorated alcohol-induced changes in histopathology, reducing the levels of alcohol metabolizing factors and aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), and total cholesterol (TC) contents, which demonstrated that antioxidants effectively mitigated liver injury in ALD mice. Further studies showed that antioxidants reversed the disruption of fatty acid (FA) synthesis and lipid transport induced by alcohol exposure, and restored phospholipid levels. Especially, Vc and NAC increased the endogenous antioxidant plasmenyl phosphatidylethanolamine (PlsEtn). Additionally, antioxidants ameliorated the alcohol-impaired mitochondrial function and inhibited excessive oxidative stress. In conclusion, antioxidants can regulate lipid metabolism and phospholipid homeostasis, which in turn inhibit oxidative stress and thereby exert protective effects against ALD.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"229-240"},"PeriodicalIF":1.9,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10211267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary and plasma retinoids are not associated with fatty acid desaturase indices in healthy young adults","authors":"Julia Hatherell,&nbsp;Salma A. Abdelmagid,&nbsp;David W. L. Ma,&nbsp;Ahmed El-Sohemy,&nbsp;David M. Mutch","doi":"10.1002/lipd.12376","DOIUrl":"10.1002/lipd.12376","url":null,"abstract":"<p>Past research in rodents suggests that fatty acid (FA) desaturase expression and activity may be modified by vitamin A; however, this has not been investigated in humans. The primary objective of this study was to examine associations between dietary retinoid intakes, plasma retinoid concentrations, and FA desaturase indices in young adults. As a secondary objective, biological sex and estrogen-containing contraceptive (EC) use were investigated due to prior evidence demonstrating that both can influence plasma retinol concentration and FA desaturase indices. Dietary retinoid intake (food frequency questionnaire), plasma retinoid concentrations (high-performance liquid chromatography–tandem mass spectrometry), plasma FA (gas chromatography), and FA desaturase indices (product-to-precursor ratios) from 945 adults recruited for the cross-sectional Toronto Nutrigenomics and Health study were analyzed. Participants were stratified into quartiles based on plasma retinol concentration and data analyzed by one-way analysis of covariance. Dietary retinoid intakes were not associated with the overall <i>n</i>-3 pathway, overall <i>n</i>-6 pathway, delta-5 desaturase, delta-6 desaturase, or delta-9 desaturase indices (all <i>r</i> &lt; 0.10, <i>p</i> &gt; 0.05). The overall <i>n</i>-6 pathway index was significantly higher (<i>p</i> = 0.0004) and the delta-5 desaturase index was significantly lower (<i>p</i> = 0.0003) in individuals with higher plasma retinol levels; however, these differences were lost when participants were grouped by biological sex and EC use. Although weak relationships were observed between plasma retinol and some FA desaturase indices in the total population, these associations appear to be driven by biological sex and EC usage rather than retinoids. We therefore find little evidence of a relationship between retinoids and FA desaturase indices in young, healthy adults.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"217-227"},"PeriodicalIF":1.9,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12376","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Very low Omega-3 Index in young healthy students from Palestine","authors":"Feras Almasri,&nbsp;Manal Badrasawi,&nbsp;Rana Zahdeh,&nbsp;Andreas Hahn,&nbsp;Jan Philipp Schuchardt,&nbsp;Theresa Greupner","doi":"10.1002/lipd.12375","DOIUrl":"10.1002/lipd.12375","url":null,"abstract":"Oily fish is rich in long-chain omega-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which have been associated with several health benefits. However, fish consumption is generally low in many countries, including the Middle East, resulting in low omega-3 blood levels. In Palestine, no data on the omega-3 blood status is available. The aim of this cross-sectional study was to assess the omega-3 status and related factors in young healthy subjects from Palestine. Omega-3 status was assessed using the Omega-3 Index-defined as the sum of EPA + DHA in relation to the total fatty acid content of erythrocytes. A total of 149 subjects, 50 males and 99 females (age range: 18-24 years), were included in the study. In addition to the Omega-3 Index, data on anthropometrics, physical activity, smoking status, fish intake, dietary supplement intake, blood lipid profile, and whole erythrocyte fatty acid pattern were collected. The mean (SD) Omega-3 Index was 2.56 (0.57)%, with 97.9% of subjects having an index below 4%. The majority of participants (91.8%) consumed less than two portions of fish per week, and only 4% reported taking omega-3 supplements, mostly irregularly. Our findings show that young Palestinian students have an alarmingly low omega-3 status. Further studies are needed to investigate whether the omega-3 status is also low in the general Palestinian population.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 5","pages":"209-216"},"PeriodicalIF":1.9,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12375","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10261973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atherogenic dyslipidaemia in type 2 diabetes mellitus: The PREDISAT study","authors":"Elisenda Climent,&nbsp;Jesús Millán,&nbsp;Juan F. Ascaso,&nbsp;Manuel Suárez-Tembra,&nbsp;Carlos Morillas,&nbsp;Fernando Civeira,&nbsp;Jose M. Bellón,&nbsp;Juan Pedro-Botet,&nbsp;the Spanish Atherosclerosis Society Working Group on Atherogenic Dyslipidaemia","doi":"10.1002/lipd.12374","DOIUrl":"10.1002/lipd.12374","url":null,"abstract":"<p>Extremely variable prevalence rates of atherogenic dyslipidaemia (AD) in type 2 diabetes (T2DM) subjects have been reported. The primary aim was to assess AD prevalence in Spanish T2DM subjects. Secondary objectives were to evaluate the differential clinical characteristics between T2DM subjects with and without AD, to describe lipid profile evolution and use of lipid-lowering treatment in clinical practice by the Spanish Lipid Units. Data was obtained from the National Registry of Dyslipidaemias of the Spanish Atherosclerosis Society, from a multicentric sub-study focused on AD prevalence in T2DM subjects (PREDISAT study). The inclusion criteria were subjects diagnosed of T2DM with age ≥18 years old. A total of 385 T2DM subjects with a mean age of 61 years and 246 (64%) men were included. The mean follow-up was 22 ± 7.4 months. At baseline, 41.3% of the T2DM subjects presented AD, this percentage decreasing to 34.8% with therapeutic intervention. AD prevalence varied in different age groups and appeared to be more prevalent in younger T2DM subjects. Those with AD had a more atherogenic lipid profile at baseline, with higher total cholesterol, triglyceride and non-(high-density lipoprotein) HDL cholesterol levels at baseline, together with lower HDL cholesterol concentrations, without achieving lipid subfraction goals during follow-up. Although almost 90% of the AD subjects were under lipid-lowering treatment, most were receiving only one drug, being statins the most used treatmentA high AD prevalence in T2DM subjects was observed, being age a determinant factor, with a modest decline during follow-up. Although almost 90% of the AD subjects were under lipid-lowering drugs, most were only receiving monotherapy with statins.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 4","pages":"197-206"},"PeriodicalIF":1.9,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9797329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous tetracosahexaenoic acid modifies the fatty acid composition of human primary T lymphocytes and Jurkat T cell leukemia cells contingent on cell type","authors":"Nicola A. Irvine,&nbsp;Annette L. West,&nbsp;Johanna Von Gerichten,&nbsp;Elizabeth A. Miles,&nbsp;Karen A. Lillycrop,&nbsp;Philip C. Calder,&nbsp;Barbara A. Fielding,&nbsp;Graham C. Burdge","doi":"10.1002/lipd.12372","DOIUrl":"10.1002/lipd.12372","url":null,"abstract":"<p>Tetracosahexaenoic acid (24:6ω-3) is an intermediate in the conversion of 18:3ω-3 to 22:6ω-3 in mammals. There is limited information about whether cells can assimilate and metabolize exogenous 24:6ω-3. This study compared the effect of incubation with 24:6ω-3 on the fatty acid composition of two related cell types, primary CD3⁺ T lymphocytes and Jurkat T cell leukemia, which differ in the integrity of the polyunsaturated fatty acid (PUFA) biosynthesis pathway. 24:6ω-3 was only detected in either cell type when cells were incubated with 24:6ω-3. Incubation with 24:6ω-3 induced similar increments in the amount of 22:6ω-3 in both cell types and modified the homeoviscous adaptations fatty acid composition induced by activation of T lymphocytes. The effect of incubation with 18:3ω-3 compared to 24:6ω-3 on the increment in 22:6ω-3 was tested in Jurkat cells because primary T cells cannot convert 18:3ω-3 to 22:6ω-3. The increment in the 22:6ω-3 content of Jurkat cells incubated with 24:6ω-3 was 19.5-fold greater than that of cells incubated with 18:3ω-3. Acyl-coA oxidase siRNA knockdown decreased the amount of 22:6ω-3 and increased the amount of 24:6ω-3 in Jurkat cells. These findings show exogenous 24:6ω-3 can be incorporated into primary human T lymphocytes and Jurkat cells and induces changes in fatty acid composition consistent with its conversion to 22:6ω-3 via a mechanism involving peroxisomal β-oxidation that is regulated independently from the integrity of the upstream PUFA synthesis pathway. One further implication is that consuming 24:6ω-3 may be an effective alternative means of achieving health benefits attributed to 20:5ω-3 and 22:6ω-3.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"58 4","pages":"185-196"},"PeriodicalIF":1.9,"publicationDate":"2023-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.12372","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9849098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}