Lipids最新文献

{"title":"ASGR1 inhibitors, inflammation, and heart failure: A Mendelian randomization analysis.","authors":"Xingsheng Ye, Miaomiao Yang, Yinghao Hong, Zhizhan Lin, Jian Peng, Liwei He","doi":"10.1002/lipd.12446","DOIUrl":"https://doi.org/10.1002/lipd.12446","url":null,"abstract":"<p><p>This study investigates the causal relationship between Asialoglycoprotein receptor 1 (ASGR1) inhibitors, inflammation, and heart failure (HF). Leveraging data from the Global Lipids Genetics Consortium (2021) and the UK Biobank, we identified instrumental variables for ASGR1 and validated these genetic instruments using coronary heart disease (CHD) patients as positive controls. We employed Instrumental variable weighted (IVW) and Summary-data-based Mendelian Randomization (SMR) methods to assess the association of ASGR1 with HF and its associated risk factors, comparing efficacy with PCSK9 and LDLR. Mediation analysis of inflammatory biomarkers was conducted using a two-step Mendelian randomization approach, with sensitivity analyses performed using conventional MR methods. IVW analysis demonstrates a significant positive correlation between ASGR1 expression and HF, myocardial infarction, non-ischemic cardiomyopathy, and calcific aortic valvular stenosis. Patients with HF comorbid with CHD showed an increased likelihood of benefit. ASGR1 exhibited a stronger effect on HF compared to PCSK9. Similar conclusions were drawn from SMR analysis. Additionally, LDLR shows no causal relationship with HF but appears negatively correlated with non-ischemic cardiomyopathy. Among the 91 inflammatory proteins studied, Leukemia inhibitory factor receptor (LIF-R) and Urokinase-type plasminogen activator (uPA) were found to mediate the effects of ASGR1 on HF. Sensitivity analyses indicate no evidence of pleiotropy in reported results. This study supports a causal association between ASGR1 inhibitors and HF, providing genetic evidence for the anti-inflammatory role of ASGR1 inhibitors in reducing HF risk. LIF-R and uPA, identified as potential mediators, introduce a novel therapeutic pathway for HF management.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantum Insights Into Fatty Acids, Antioxidant Mechanisms, and Molecular Docking Studies in Anacamptis papilionacea.","authors":"Taşkın Basılı, Erdi Can Aytar, Mayker Lazaro Dantas Miranda, Bengisu Şentürk, Yasemin Özdener Kömpe","doi":"10.1002/lipd.12448","DOIUrl":"https://doi.org/10.1002/lipd.12448","url":null,"abstract":"<p><p>This study examines the antioxidant activities, fatty acid composition, cytotoxicity predictions, and endocrine-disrupting potential of metabolites from orchid methanolic-flower extracts. In silico analyses, including molecular docking, NCI-RDG, FMO, ELF, LOL, and MEP mapping, were conducted to explore noncovalent interactions, electronic properties, reactivity, and charge distribution of key metabolites, providing insights into their bioactivity and mechanisms of action. Antioxidant activity, evaluated via the DPPH assay, showed an IC₅₀ value of 12.83 mg/mL for the methanolic extract, outperforming the positive control (BHT, IC₅₀ = 18.00 mg/mL). High flavonoid content, expressed as quercetin equivalents, confirmed the presence of bioactive compounds, including quercetin and gallic acid. The fatty acid (FA) profile of Anacamptis papilionacea fixed oil, analyzed by GC-MS, revealed palmitic acid, α-linolenic acid, 8,11,14-eicosatrienoic acid, and docosahexaenoic acid as dominant FAs, constituting 79.47% of the total oil. Molecular docking showed strong binding interactions of these FAs with the human glucocorticoid receptor. Cytotoxicity predictions indicated promising activity against melanoma and lung carcinoma cells, particularly with 8,11,14-eicosatrienoic acid and docosahexaenoic acid. Endocrine-disrupting potential was evaluated through molecular binding with 14 nuclear receptors, showing varying affinities, particularly for estrogen, androgen, and glucocorticoid receptors. These findings highlight the bioactivity and therapeutic potential of A. papilionacea metabolites, emphasizing their antioxidant, cytotoxic, and receptor-binding capabilities. Further experimental studies are warranted for clinical validation.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthropometric indicators of obesity as screening tools for low high-density lipoprotein cholesterol in older adults: A cross-sectional study.","authors":"Yuri Silva de Souza, Lucas Dos Santos, Débora Jesus da Silva, Emille Silva Santos, Sabrina da Silva Caires, Paulo da Fonseca Valença Neto, Claudio Bispo de Almeida, Pabline Dos Santos Santana, Raquel Dos Santos Barbosa, Cezar Augusto Casotti","doi":"10.1002/lipd.12447","DOIUrl":"https://doi.org/10.1002/lipd.12447","url":null,"abstract":"<p><p>Low levels of high-density lipoprotein cholesterol (HDL-C) increase the risk of cardiovascular events and mortality in older individuals. Developing cost-effective screening tools for identifying this condition in large populations is essential. To evaluate the discriminatory capacity of anthropometric indicators of obesity in screening for low HDL-C among older adults. A cross-sectional population-based epidemiological study was conducted with 223 older individuals (57% women) living in a community. The following anthropometric indicators were evaluated: body mass index (BMI), waist circumference (WC), abdominal circumference (AC), body adiposity index (BAI), triceps skinfold (TSF), waist-hip ratio (WHR), waist-height ratio (WHtR), and conicity index (CIn). Low HDL-C was defined as values below 40 mg/dL. The prevalence of low HDL-C was 25.10% (men: 35.41%; women: 21.77%). Among older men, BAI showed the highest sensitivity (87.10%; accuracy: 0.70; 95% CI: 0.53-0.74), while WC had the highest specificity (83.05%; accuracy: 0.71; 95% CI: 0.60-0.80). Among older women, WHR demonstrated the highest sensitivity (100%; accuracy: 0.71; 95% CI: 0.62-0.79), and WHtR showed the highest specificity (67.68%; accuracy: 0.71; 95% CI: 0.62-0.79). BAI and WHR were the most effective indicators for screening older men and women with low HDL-C, respectively. Additionally, WC and WHtR were the most effective indicators for identifying older men and women without low HDL-C, respectively.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of low-dose atorvastatin plus ezetimibe for primary hypercholesterolemia: A randomized, double-blind, multicenter phase 3 trial.","authors":"Tae Oh Kim, Kyounghoon Lee, Jin-Man Cho, Hyuck-Jun Yoon, Tae-Ho Park, Jung Hyun Choi, Jung-Won Suh, Seok-Yeon Kim, Hong-Seok Lim, Jong-Seon Park, Deok-Kyu Cho, Gyung-Min Park, Sung-Gyun Ahn, Sanghoon Shin, Sung Uk Kwon, Dae-Hyeok Kim, Sang-Rok Lee, Jung-Hoon Sung, Hwan-Cheol Park, Seung-Whan Lee","doi":"10.1002/lipd.12442","DOIUrl":"https://doi.org/10.1002/lipd.12442","url":null,"abstract":"<p><p>Studies have suggested that low-dose statin monotherapy may be insufficient for target LDL-C levels. In this randomized, double-blind, multicenter phase 3 trial, we evaluated the efficacy of combined ezetimibe and low-dose atorvastatin in 222 Korean patients with primary hypercholesterolemia. Participants received either 10-mg ezetimibe/5-mg atorvastatin (EZE10/ATV5), 10-mg ezetimibe (EZE10), 5-mg atorvastatin (ATV5), or 10-mg atorvastatin (ATV10). At 8 weeks, EZE10/ATV5 achieved the greatest LDL-C reduction (-44.8%) compared with EZE10 (-12.7%, p < 0.0001), ATV5 (-27.3%, p < 0.0001), and ATV10 (-32.0%, p = 0.0012). The combination therapy showed the highest LDL-C goal achievement rate (41.1% vs. EZE10 8.9%, p < 0.0001; ATV5 10.9%, p < 0.0001; ATV10 27.3%, p = 0.0342), particularly in moderate to high-risk patients. Additionally, EZE10/ATV5 had the lowest adverse events among all groups (6.9% vs. 15.0%, 12.3%, and 27.6%, p = 0.017), with most events being mild. These findings suggest that the combination of ezetimibe and low-dose atorvastatin provides superior lipid-lowering efficacy with an improved safety profile, offering an effective treatment for primary hypercholesterolemia in Korean patients.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous oxidized phytosterol may modulate linoleic acid metabolism through upregulation of fatty acid desaturase in rats.","authors":"Tomonari Koyama, Kyoichi Osada","doi":"10.1002/lipd.12444","DOIUrl":"https://doi.org/10.1002/lipd.12444","url":null,"abstract":"<p><p>Previous in vitro studies have indicated that oxidized phytosterol (OPS) exhibits some toxicity; however, the harmful effects of OPS on fatty acid metabolism are not completely understood yet. Therefore, this study examined the effects of exogenous phytosterol (PS) and OPS on growth parameters and lipid metabolism in rats. Rats were provided with AIN-76 basal diet, basal diet +0.5% PS, or basal diet +0.5% OPS. We found that the level of cholesterol and triacylglycerols in the liver was significantly lower in OPS-fed rats than in basal diet-fed rats. The ratio of Δ6 desaturation index (20:3(n-6) + 20:4(n-6))/18:2(n-6) in the plasma was significantly higher in the OPS-fed rats than in the PS-fed rats. Additionally, the proportion of arachidonic acid (20:4) in the liver was significantly higher in the OPS-fed group compared with the control group. The mRNA expression levels of Δ6 and Δ5 desaturases were significantly higher in OPS-fed rats than in basal diet-fed rats, but remained unchanged in PS-fed rats. Moreover, the protein level of Δ6 desaturase was significantly higher in both PS- and OPS-fed rats compared with basal diet-fed rats, while the protein level of Δ5 desaturase tended to be higher only in OPS-fed rats than in basal diet-fed rats. Thus, exogenous OPS, but not PS, altered fatty acid composition through the upregulation of mRNA and protein levels of fatty acid desaturation enzymes in the liver. This indicates that exogenous OPS, unlike PS, may modulate the production of eicosanoids from arachidonic acid, potentially promoting allergic reactions, inflammation, and atherosclerosis.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid and lipoprotein particle profiles in patients with type 2 diabetes mellitus: A comparative study between highlanders and lowlanders.","authors":"Chenghui Zhang, Rong Du, Suyuan Wang, Yan Ye, Xueping Sun, Yunhong Wu, Mingxia Li","doi":"10.1002/lipd.12441","DOIUrl":"https://doi.org/10.1002/lipd.12441","url":null,"abstract":"<p><p>The lipid profiles of highlanders and lowlanders differ. However, studies of the lipid profiles of patients with type 2 diabetes mellitus (T2DM) living at high altitudes are limited. This study aimed to compare lipid profiles and lipoprotein particles in individuals with T2DM permanently residing in the Tibetan Plateau and those residing in the lowlands. This cross-sectional study included 117 individuals with T2DM from the lowlands (n = 78) and highlands (n = 39). Demographic information, clinical features, blood biochemical indices, and standard lipid concentrations were recorded and evaluated. Low-density lipoprotein particles (LDL-Ps) and LDL subfractions were compared between the groups. Additionally, the risk for atherosclerotic cardiovascular disease (ASCVD) was assessed. The standard lipid profile and nuclear magnetic resonance-measured lipid panel showed no significant differences in terms of triglyceride, total cholesterol, and high-density lipoprotein cholesterol levels. However, highlanders exhibited a higher mean level of LDL cholesterol (LDL-C) than lowlanders. The LDL-P mean level was significantly higher in highlanders than in lowlanders. Small-density LDL (sdLDL) showed no distinct difference between the two groups. Furthermore, the 10-year risks of ASCVD were slightly higher in highlanders than in lowlanders, although the difference was not statistically significant. Individuals with T2DM living at highland altitudes have higher LDL-C and LDL-P levels than those living in lowlands. No difference was observed in the sdLDL levels between the two populations. These discrepancies may carry profound implications for the clinical management of T2DM and the prevention of cardiovascular diseases in high-altitude areas.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysophosphatidylcholine, rich in n-3 polyunsaturated fatty acids, inhibits leukotriene production by mouse mast cells.","authors":"Mikako Takasugi, Hirofumi Arai, Koki Sugimoto, Tadahiro Tsushima, Ryota Hosomi, Kenji Fukunaga, Koretaro Takahashi","doi":"10.1002/lipd.12443","DOIUrl":"https://doi.org/10.1002/lipd.12443","url":null,"abstract":"<p><p>In fishery industries, large amounts of byproducts are discarded during processing; therefore, their use as a source of n-3 polyunsaturated fatty acids (PUFAs) is beneficial. In this study, we evaluated the potential anti-allergic effect of lysophosphatidylcholine (Lyso-PtdCho) derived from squid skin phosphatidylcholine (PtdCho) by measuring the production of leukotriene (LT) B₄ and C₄, which are chemical mediators produced by mast cells in immediate allergic reactions. Squid Lyso-PtdCho, with docosahexaenoic acid exclusively esterified at the sn-2 position, significantly inhibited LT production, whereas the original PtdCho obtained from squid skin showed no inhibitory activity. Lyso-PtdCho prepared from starfish viscera PtdCho, which exclusively binds eicosapentaenoic acid at the sn-2 position, also inhibited LT production, although the effect was moderate compared with that of the squid Lyso-PtdCho. It has been suggested that Lyso-PtdCho containing n-3 PUFA may alleviate allergic symptoms and contribute to the effective utilization of fishery wastes and processing byproducts.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonlinear association between plasma elaidic acid level and sleep complaints in US adults: NHANES 2009-2010.","authors":"Xin-Ying Liu, Kai Yao","doi":"10.1002/lipd.12445","DOIUrl":"https://doi.org/10.1002/lipd.12445","url":null,"abstract":"<p><p>Poor sleep is linked to an increased risk of metabolic, cardiovascular, psychiatric, and neurodegenerative diseases, highlighting its emergence as a public health concern. Previous studies demonstrated the harmful effects of trans fatty acids (TFAs) on human health. However, the association between TFAs and sleep outcomes is still not well-established. The current study aimed to investigate the relationship between plasma elaidic acid, a major TFA, and sleep complaints. The participants from the US National Health and Nutrition Examination Survey (NHANES) (2009-2010) were included. The plasma concentration of elaidic acid (18:1n-9t) was determined using the gas chromatography/mass spectrometry (GC/MS) method. The sleep outcome was defined based on the following questionnaire: \"Ever told the doctor had trouble sleeping?\" and \"Ever told by a doctor have a sleep disorder?\". Participants were classified as having sleep complaints if they ever told a doctor or been told by a doctor about trouble sleeping. The association between plasma elaidic acid and sleep complaints was investigated by multivariable logistic regressions, restricted cubic spline (RCS), and subgroup analysis. A total of 2068 participants were included, 561 of whom suffered from sleep complaints. The restricted cubic spline (RCS) regression analysis revealed a nonlinear (inverted L-shaped) relationship between plasma elaidic acid level and sleep complaints (p = 0.044), with an inflection point of 9.598 μmol/L. In the group with a low plasma elaidic acid level (≤9.598 μmol/L), there was a positive association between plasma elaidic acid level and the prevalence of sleep complaints (OR 1.28; 95% CI: 1.06-1.54, p = 0.01). When the plasma elaidic acid level was more than 9.598 μmol/L, the correlation was not statistically significant. Results were robust when analyses were stratified by sex, age, race, marital status, education level, family income, and BMI (all p for interaction >0.05). The results revealed a positive association between plasma elaidic acid and sleep complaints in US adults when plasma elaidic acid level ≤9.598 μmol. Given that the plasma TFA content has considerably declined in recent years, the detrimental impact of elaidic acid on sleep quality deserves more attention.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a high-fat diet on gut microbiota and possible implications for bone health in male Wistar rats.","authors":"Fernanda Guedes Rodrigues, Milene Subtil Ormanji, Renata Meca, Horácio Montenegro, Lilian Cuppari, Martin H de Borst, Ita Pfeferman Heilberg","doi":"10.1002/lipd.12440","DOIUrl":"https://doi.org/10.1002/lipd.12440","url":null,"abstract":"<p><p>Diet plays an important role in the composition of gut microbiota. Emerging research suggests that bone homeostasis can also be influenced by the gut microbiota. The aim of this study was to assess possible alterations in gut microbiota in an experimental obesity model induced by a high-fat diet (HFD) and the possible effects on parameters of bone metabolism and remodeling. Male Wistar rats were fed a HFD (60% lipids) or standard (control) diet for 14 weeks. Biochemical and hormonal parameters, bone histomorphometry, bone protein levels, and gut microbiota composition were analyzed. HFD animals exhibited a greater gut microbiota α-diversity represented by the Shannon Index and an increased relative abundance of the Proteobacteria phylum. Histomorphometry detected lower bone formation in the HFD group, accompanied by increased levels of serum and bone leptin and FGF-23 (fibroblast growth factor-23). The Shannon Index was correlated directly with bone FGF-23 (R 0.96, p = 0.04) and inversely with the osteoblastic surface (R -0.95, p = 0.04). The present study disclosed a significant increase in gut microbiota α-diversity and relative abundance of Proteobacteria phylum in obese animals fed a high-fat diet in parallel with increased levels of bone and serum leptin and FGF-23 and lower bone formation. The associations of Shannon Index with bone levels of FGF-23 and reduced osteoblastic surface suggest a link between HFD-induced higher gut microbiota diversity and low bone formation.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-regression of the relationship between dietary 18C n-3 concentration and enrichment of n-3 fatty acids in liver and breast muscle of broiler chickens.","authors":"Usman Ali, Ahmed El-Zenary, Kevin J Harvatine","doi":"10.1002/lipd.12439","DOIUrl":"https://doi.org/10.1002/lipd.12439","url":null,"abstract":"<p><p>The long-chain n-3 fatty acids (FA) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have human health benefits, and many experiments have investigated the ability of plant n-3 sources to enrich n-3 FA in broiler meat. A meta-regression was conducted to evaluate the relationship between dietary 18-carbon n-3 FA content and the deposition of EPA, docosapentaenoic acid (DPA), and DHA in broiler breast and liver tissue. Bivariate regression of total diet 18-carbon n-3 and tissue FA profile was modeled with the random effect of experiment and the partial R² was calculated. Increasing 18C n-3 FA in the diet quadratically increased the log₁₀ concentration of all n-3 FA in breast tissue. The strongest relationship was found for breast alpha-linolenic (partial R² = 0.55), followed by EPA, DPA, and DHA (partial R² = 0.20, 0.14, and 0.05). Liver enrichment of EPA, DPA, and DHA (all partial R² > 0.43) and EPA + DHA (partial R² = 0.55) was quadratically related to dietary 18C n-3 FA, while liver EPA + DPA + DHA (partial R² = 0.43) was linearly related to dietary 18C n-3 FA. In both breast and liver, the plateau in tissue enrichment was more apparent for DHA than EPA, with DPA being intermediate. A linear plateau model fit the data with a break point for enriching EPA and DHA in breast muscle at 22.4 and 17.9 g/kg of 18C n-3 FA in the diet, respectively. Enrichment of long-chain n-3 FA in broiler chickens was saturable, with little justification for feeding beyond approximately 20 g/kg 18C n-3 FA.</p>","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}