The Binding Strength of Cytochrome C to Membranes Containing Phosphatidic Acid Corresponds to Its Peroxidase Activity.

IF 1.8 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Lipids Pub Date : 2025-06-17 DOI:10.1002/lipd.12451

G O Stepanov, V V Zakharov, A A Struchkova, E D Rumyantseva, V V Volkov, A Yu Makarov, Yu A Vladimirov, A N Osipov

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Abstract

The study of the molecular mechanisms of programmed cell death and the role of anionic phospholipids like cardiolipin in these processes is one of the most attractive topics in modern lipidomics. The aim of this study was the quantitative assessment of the presence of phosphatidic acid in the binding strength of cytochrome C to biological membranes, its peroxidase activity, and the role of phospholipase D in increasing the concentration of phosphatidic acid. Using the Langmuir method for fluorescence quenching analysis (K_d = 2.78 × 10^-7 M) of the cytochrome C-phosphatidic acid complex was shown. The presence of phosphatidic acid (both synthetic and obtained from phosphatidylcholine using phospholipase D from mitochondrial lipids) increased the peroxidase activity of cytochrome C more than 100-fold (measured by luminol-enhanced chemiluminescence method). The obtained results emphasize the possibility of participation not only of cardiolipin but also of phosphatidic acid in the development of molecular proapoptotic processes.

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细胞色素C与磷脂酸膜的结合强度与其过氧化物酶活性相对应。

研究细胞程序性死亡的分子机制以及阴离子磷脂如心磷脂在这一过程中的作用是现代脂质组学中最具吸引力的课题之一。本研究的目的是定量评估磷脂酸的存在对细胞色素C与生物膜的结合强度、过氧化物酶活性以及磷脂酶D在增加磷脂酸浓度中的作用。用Langmuir法对细胞色素c -磷脂酸络合物进行了荧光猝灭分析（Kd = 2.78 × 10-7 M）。磷脂酸的存在（无论是合成的还是从磷脂酰胆碱中提取的，使用线粒体脂质的磷脂酶D）使细胞色素C的过氧化物酶活性增加了100倍以上（通过发光醇增强化学发光法测量）。所获得的结果强调了不仅心磷脂而且磷脂酸参与分子促凋亡过程的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lipids 生物-生化与分子生物学

CiteScore

4.20

自引率

5.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Lipids is a journal of the American Oil Chemists'' Society (AOCS) that focuses on publishing high-quality peer-reviewed papers and invited reviews in the general area of lipid research, including chemistry, biochemistry, clinical nutrition, and metabolism. In addition, Lipids publishes papers establishing novel methods for addressing research questions in the field of lipid research.