Exploring the Lipid-Reducing Potential of Elsholtzia bodinieri Vaniot Essential Oil: Implications for HepG2 Cells and the Caenorhabditis elegans Obesity Models.

IF 1.8 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Lipids Pub Date : 2025-06-08 DOI:10.1002/lipd.12450

Xiaoying Liu, Mengyuan Zhang, Runyuan Wang, Fang Xu, Hua Bai, Wei Zou, Qi Wang

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Abstract

Elsholtzia bodinieri Vaniot, a medicinal and edible plant native to Yunnan, China, was investigated for its lipid-reducing effects in this study. The essential oil of E. bodinieri Vaniot (EBVEO) demonstrated a significant reduction in lipid droplets, intracellular triglyceride (TG), and total cholesterol (TC) levels in HepG2 lipid-accumulating cells. EBVEO upregulated the expression of key proteins, including SIRT1, AMPK, PPARα, and CD36, promoting enhanced β-oxidation and fat breakdown. This regulatory effect on lipid metabolism was also observed in a high-fat Caenorhabditis elegans (C. elegans) model. qPCR and parallel reaction monitoring (PRM) revealed the activation of the AAK-2/NHR-49 pathway, homologs of PPARα and AMPK, leading to a substantial increase in fat oxidation and a notable reduction in fat accumulation in C. elegans. In conclusion, EBVEO exhibits promising potential in regulating cellular and C. elegans lipid metabolism through the SIRT1/AMPK and AAK-2/NHR-49 pathways, positioning it as a promising lipid metabolism enhancer.

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探索香草精油的降脂潜能：对HepG2细胞和秀丽隐杆线虫肥胖模型的影响。

本研究对原产于中国云南的药用和食用植物“香叶”的降脂作用进行了研究。在HepG2脂质积聚细胞中，EBVEO精油能显著降低脂滴、细胞内甘油三酯（TG）和总胆固醇（TC）水平。EBVEO上调关键蛋白的表达，包括SIRT1、AMPK、PPARα和CD36，促进β-氧化和脂肪分解。在高脂肪秀丽隐杆线虫（C. elegans）模型中也观察到这种对脂质代谢的调节作用。qPCR和平行反应监测（PRM）显示，AAK-2/NHR-49通路、PPARα和AMPK的同源物被激活，导致秀丽隐杆线虫脂肪氧化显著增加，脂肪积累显著减少。综上所述，EBVEO在通过SIRT1/AMPK和AAK-2/NHR-49通路调节细胞和秀丽隐杆线虫脂质代谢方面表现出良好的潜力，是一种有前景的脂质代谢促进剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lipids 生物-生化与分子生物学

CiteScore

4.20

自引率

5.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Lipids is a journal of the American Oil Chemists'' Society (AOCS) that focuses on publishing high-quality peer-reviewed papers and invited reviews in the general area of lipid research, including chemistry, biochemistry, clinical nutrition, and metabolism. In addition, Lipids publishes papers establishing novel methods for addressing research questions in the field of lipid research.