IF 1.6 4区医学

Lipids Pub Date : 2026-08-25 DOI: 10.1002/lipd.70082

Hamzah Pratama Megantara, Naura Anjanie, Joeristanti Soelistyaningroem

{"title":"PCSK9 Inhibition Lowers LDL Cholesterol After Heart Transplantation: An Updated Systematic Review and Meta-Analysis.","authors":"Hamzah Pratama Megantara, Naura Anjanie, Joeristanti Soelistyaningroem","doi":"10.1002/lipd.70082","DOIUrl":"https://doi.org/10.1002/lipd.70082","url":null,"abstract":"Lipid management after heart transplantation (HT) is complicated by post-transplant dyslipidemia, immunosuppression, statin intolerance, and cardiac allograft vasculopathy (CAV). Proprotein convertase subtilisin/kexin Type 9 (PCSK9) inhibitors lower LDL-C in non-transplant populations, but HT recipients were under-represented in pivotal lipid trials. This updated systematic review and exploratory meta-analysis synthesized full-text evidence of PCSK9 inhibition after adult HT, predominantly with alirocumab or evolocumab. LDL-C lowering was the primary lipid outcome. CAV imaging/physiology, rejection, donor-specific antibodies, immunosuppression stability, and safety were secondary outcomes. PubMed/MEDLINE, Scopus, Cochrane Library, and Google Scholar were searched through June 20, 2026. Sixteen primary studies were included. Two independent randomized trials, EVOLVD and CAVIAR, both conducted during the first post-transplant year, provide the highest-quality evidence. The remaining evidence consists of an EVOLVD physiology/OCT substudy and observational or case-based reports. Ten studies contributed LDL-C effect and variance data. The exploratory random-effects summary estimated an LDL-C reduction of 73.6 mg/dL (95% CI, 58.8-88.4, I2 = 89.8%). The randomized-trial subgroup showed a smaller but consistent reduction of 41.8 mg/dL (95% CI, 35.9-47.7). Observational estimates were larger but derived from selected, lower-certainty cohorts. Randomized first-year evidence did not show clear improvement in CAV imaging or coronary physiology. PCSK9 inhibition lowers LDL-C after HT and appears tolerable, but CAV modification and graft-outcome benefit remain unproven.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Extraction Techniques and Low-Temperature Fractionation on the Fatty Acid Composition, Bioactive, and Antioxidant Activity of Chia Seed Oil. 提取工艺及低温分馏对奇亚籽油脂肪酸组成、生物活性及抗氧化活性的影响。

IF 1.6 4区医学

Lipids Pub Date : 2026-08-17 DOI: 10.1002/lipd.70081

Arun Kumar Shakya, Navdeep Jindal, Charanjit S Riar

{"title":"Effect of Extraction Techniques and Low-Temperature Fractionation on the Fatty Acid Composition, Bioactive, and Antioxidant Activity of Chia Seed Oil.","authors":"Arun Kumar Shakya, Navdeep Jindal, Charanjit S Riar","doi":"10.1002/lipd.70081","DOIUrl":"https://doi.org/10.1002/lipd.70081","url":null,"abstract":"In the present study, black and white chia seed oils were extracted using different novel (supercritical fluid extraction & SFE) and assisted green extraction techniques (ultrasound-assisted solvent extraction & UASE, conventional solvent extraction & CSE), followed by fractionation applying low-temperature crystallization. The oils were fractionated at temperatures ranging from -15°C to -30°C to obtain olein and stearin fractions. The effect of extraction methods and subsequent winterisation on the physicochemical properties, fatty acid profile, antioxidant content, and oxidative stability of the oil fractions was evaluated. Among the tested temperatures, -25°C produced the higher olein yield and iodine value (IV) for both the seed types. Winterisation significantly modified the composition of the olein fraction, increased the refractive index (1.481), iodine value (219.73 g I2/100 g), and the polyunsaturated fatty acids, particularly α-linolenic acid (71.12%), linoleic acid (19.83%), and oleic acid (5.95%) in black chia seed oil. The olein fraction also showed higher concentrations of antioxidants, including total phenolics (43.85 mg GAE/100 g), total tocopherol (63.99 mg/100 g), and total carotenoid (57.76 mg/kg), than the corresponding stearin fraction. Despite a decrease in the induction time of the olein fraction (0.97-0.75 h) corresponding to raw oils, the increased degree of unsaturation outweighed the protective effect of the retained endogenous antioxidants under accelerated oxidation conditions. In conclusion, winterisation effectively concentrated omega-3-rich and antioxidant compounds in the olein fraction, which preferably enhanced its nutritional value and functionality for potential food, cosmetic, and nutraceutical applications.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148795195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RARRES2 Knockdown Attenuates the Partial Macrophage-Like Phenotype and Dysfunction of Vascular Smooth Muscle Cells in an In Vitro Atherosclerosis Model via the NF-κB/NLRP3 Pathway. RARRES2敲低可通过NF-κB/NLRP3途径减弱体外动脉粥样硬化模型中部分巨噬细胞样表型和血管平滑肌细胞功能障碍。

IF 1.6 4区医学

Lipids Pub Date : 2026-08-10 DOI: 10.1002/lipd.70078

Xujiao Hu, Yu Fang

{"title":"RARRES2 Knockdown Attenuates the Partial Macrophage-Like Phenotype and Dysfunction of Vascular Smooth Muscle Cells in an In Vitro Atherosclerosis Model via the NF-κB/NLRP3 Pathway.","authors":"Xujiao Hu, Yu Fang","doi":"10.1002/lipd.70078","DOIUrl":"https://doi.org/10.1002/lipd.70078","url":null,"abstract":"In atherosclerosis (AS), the conversion of vascular smooth muscle cells (VSMCs) to a macrophage-like phenotype is a key contributor to plaque instability. Although retinoic acid receptor responder 2 (RARRES2) has been identified as an adipokine implicated in inflammation and metabolism, its role in VSMC phenotypic transformation remains unknown. This study aimed to investigate whether RARRES2 regulates the macrophage-like transformation and dysfunction of VSMCs. In the in vitro AS model, RARRES2 expression was significantly upregulated. Knockdown of RARRES2 inhibited VSMC proliferation and migration, reduced the secretion of inflammatory factors, decreased lipid accumulation, and the number of CD68-positive cells, and suppressed the activation of key proteins in the NF-κB/NLRP3 pathway. In the AS model, RARRES2 expression was found to be significantly upregulated. Knockdown of RARRES2 inhibited the proliferation and migration of VSMCs, reduced the secretion of TNF-α, IL-6, IL-1β, and MMP-2, reduced lipid accumulation, decreased the number of CD68-positive cells (indicating an acquisition of partial macrophage-like features, though further lineage markers are needed to confirm full partial macrophage-like phenotype), and inhibited the activation of key proteins (p-p65, NLRP3, ASC) in the NF-κB/NLRP3 pathway. RARRES2 knockdown suppresses NF-κB/NLRP3 signaling and attenuates macrophage-like transformation, inflammatory responses, and lipid accumulation in VSMCs, suggesting that RARRES2 may represent a potential therapeutic target for AS.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer Activity of Liposomes Prepared Solely From Cardiolipin. 心磷脂脂质体的抗癌活性研究。

IF 1.6 4区医学

Lipids Pub Date : 2026-08-10 DOI: 10.1002/lipd.70075

Arisa Sakamoto, Ippei Noboruo, Hiroshi Tateishi, Sho Tomita, Tomoki Fukuda, Kazumi Matsushima-Nagata, Manabu Aoki, Daichi Kusakari, Takuya Masunaga, Nao Nishimura, Takaaki Ito, Masami Otsuka, Mikako Fujita, Kensaku Anraku

{"title":"Anticancer Activity of Liposomes Prepared Solely From Cardiolipin.","authors":"Arisa Sakamoto, Ippei Noboruo, Hiroshi Tateishi, Sho Tomita, Tomoki Fukuda, Kazumi Matsushima-Nagata, Manabu Aoki, Daichi Kusakari, Takuya Masunaga, Nao Nishimura, Takaaki Ito, Masami Otsuka, Mikako Fujita, Kensaku Anraku","doi":"10.1002/lipd.70075","DOIUrl":"https://doi.org/10.1002/lipd.70075","url":null,"abstract":"Despite notable medical advancements, the continuous development of novel cancer therapies remains imperative. In this study, we aimed to evaluate the anticancer activity of liposomes prepared solely from nonnatural myristoylated (saturated C14 chains) or natural cardiolipin to develop a cancer therapies strategy. We calculated IC50 values using a cell proliferation inhibition assay and investigated the underlying mechanisms in cells exhibiting high inhibitory activity using Western blotting and flow cytometry. Calculation of the IC50 revealed that liposomes prepared solely with natural cardiolipin exhibited low toxicity to normal cells (> 100 μM) and showed the strongest toxicity against cancer cells, specifically KMS-12-PE (multiple myeloma cells) (23.5 ± 11.4 μM) among various cancer cells. In contrast, liposomes prepared solely with myristoylated cardiolipin exhibited weak anticancer activity. Furthermore, the mechanism of anticancer activity was evaluated using immunoblotting and flow cytometry. After administration of natural cardiolipin liposomes, CASPASE-3 cleavage was confirmed in KMS-12-PE cells, and the annexin V-positive rate was higher than in the liposome-free control group, suggesting apoptosis induction. These results indicate that natural cardiolipin is nontoxic to normal cells and possesses anticancer activity. This study demonstrated that liposomes formulated with cardiolipin can induce anticancer activity independently. This new finding is considered a foundation for future therapeutic strategies.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolution-Oriented Immunopharmacological Potential of the Lipid Matrix of Rhynchophorus palmarum Larvae in Viral Respiratory Infections: A Perspective Review. 掌纹蝇幼虫脂质基质在病毒性呼吸道感染中的免疫药理学研究进展

IF 1.6 4区医学

Lipids Pub Date : 2026-08-05 DOI: 10.1002/lipd.70077

W D Lituma-González, C Casella, S J Ballaz

{"title":"Resolution-Oriented Immunopharmacological Potential of the Lipid Matrix of Rhynchophorus palmarum Larvae in Viral Respiratory Infections: A Perspective Review.","authors":"W D Lituma-González, C Casella, S J Ballaz","doi":"10.1002/lipd.70077","DOIUrl":"https://doi.org/10.1002/lipd.70077","url":null,"abstract":"This review explores how the fatty acid (FA) profile of the Amazonian Rhynchophorus palmarum larva (Chontacuro), traditionally eaten for respiratory ailments, might biochemically mitigate viral respiratory infections. During metamorphosis, this insect utilizes its unique FA matrix to survive severe, self-inflicted oxidative and inflammatory tissue stress. The authors hypothesize that when consumed by humans, these insect lipids undergo chylomicron-mediated lymphatic transport. This pathway bypasses initial liver metabolism, delivering unsaturated FAs directly to the pulmonary microvasculature and alveolar macrophages in the lungs. Once there, the FAs are proposed to act via two main mechanisms: One is inflammation resolution, where the ω-3 fraction is hypothesized to be metabolized via the 15-LOX enzyme pathway into Specialized Pro-Resolving Mediators (SPMs) and activate PPAR-α, potentially shifting the immune response from chronic inflammation toward active tissue repair. The other is viral sabotage: The highly unsaturated fats increase cell membrane fluidity, destabilizing the \"lipid rafts\" that viruses rely on for cellular entry and assembly. While in vitro and in silico models suggest a synergistic \"cocktail effect\" that primes and then resolves the immune response, the authors note that direct human clinical evidence is currently lacking.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148679026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating Omega-3 Fatty Acids and Cardiometabolic Outcomes: A Systematic Review of Publications From UK Biobank. 循环Omega-3脂肪酸和心脏代谢结果：对英国生物银行出版物的系统回顾。

IF 1.6 4区医学

Lipids Pub Date : 2026-07-29 DOI: 10.1002/lipd.70076

Warda Tul Firdous, Philip C Calder

{"title":"Circulating Omega-3 Fatty Acids and Cardiometabolic Outcomes: A Systematic Review of Publications From UK Biobank.","authors":"Warda Tul Firdous, Philip C Calder","doi":"10.1002/lipd.70076","DOIUrl":"https://doi.org/10.1002/lipd.70076","url":null,"abstract":"Studies have shown that omega-3 fatty acids may protect against cardiovascular diseases (CVDs) and related metabolic conditions. UK Biobank is a large cohort study that includes data on plasma omega-3 fatty acids at study entry and health outcomes during the follow-up period. The aim of this research is to explore the association between plasma omega-3 fatty acids (total omega-3 fatty acids, docosahexaenoic acid (DHA) and non-DHA omega-3 fatty acids) and cardiometabolic health outcomes by systematically reviewing publications from UK Biobank. Three databases (EMBASE, MEDLINE, CINAHL) were searched in September 2025 using key words. Based on inclusion/exclusion criteria, 16 publications were selected for inclusion (two cross-sectional and 14 longitudinal with a follow-up of 11 to 14 years). Sample size varied between 20,338 and 273,834 across the included studies. Eleven studies reported on CVD outcomes and five on metabolic outcomes. Plasma total omega-3 fatty acids and DHA were inversely associated with risk of coronary heart disease, CVD mortality, heart failure, haemorrhagic stroke and severe fatty liver disease. Plasma total omega-3 fatty acids were also inversely associated with risk of prediabetes and type-2 diabetes, while plasma DHA was also inversely associated with risk of coronary heart disease mortality, ischemic stroke and fatty liver disease. It is concluded that publications based on UK Biobank consistently demonstrate a significant association between higher levels of total omega-3 fatty acids or DHA in plasma and lower likelihood of poor cardiometabolic health outcomes, providing support for guidance for the population to achieve recommended intakes of these fatty acids.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148603916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation Between Obesity and Lipid Profile in Patients With Type 2 Diabetes Mellitus and Hypothyroidism. 2型糖尿病和甲状腺功能减退患者肥胖与血脂的相关性

IF 1.6 4区医学

Lipids Pub Date : 2026-07-20 DOI: 10.1002/lipd.70074

Rondik O Naif, Chinar M Mohammed

{"title":"Correlation Between Obesity and Lipid Profile in Patients With Type 2 Diabetes Mellitus and Hypothyroidism.","authors":"Rondik O Naif, Chinar M Mohammed","doi":"10.1002/lipd.70074","DOIUrl":"https://doi.org/10.1002/lipd.70074","url":null,"abstract":"Insufficient physical activity, poor dietary practices, escalating stress levels, and environmental factors are all contributors to the swift rise in obesity, type 2 diabetes mellitus (T2DM), and hypothyroidism worldwide. This study aimed to assess the effects of obesity on lipid profiles, glycemic control, and thyroid function in individuals with T2DM and hypothyroidism. The study included 154 participants: 100 T2DM patients with hypothyroidism and 54 healthy controls. Anthropometric measurements were collected, and biochemical tests, including fasting blood glucose (FBG), hemoglobin A1c (HbA1c), lipid profiles, and thyroid hormone levels; thyroid-stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3) were performed. The groups differed significantly (p < 0.05) in age, body mass index (BMI), FBG, HbA1c, TSH, FT3, total cholesterol, and triglycerides. Compared to patients without obesity and controls, diabetic patients with obesity showed higher levels of FBG, HbA1c, cholesterol, triglycerides, and more notable changes in thyroid hormones. Age, FBG, HbA1c, and total cholesterol were found to be significant predictors of obesity by receiver operating characteristic (ROC) curve analysis. The results demonstrate that people suffering from coexisting diabetes, hypothyroidism, and obesity present compromised glycemic control, lipid profile alterations, and thyroid dysfunction. This identifies a metabolic assessment as a primary tool in the management of obese diabetics.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DHA-Deficient Diet Affects the n-3 LC PUFA Molecular Synthesis Pathway and Fatty Acid Composition in European Sardine (Sardina pilchardus). dha缺乏对沙丁鱼n-3 LC多聚脂肪酸分子合成途径和脂肪酸组成的影响

IF 1.6 4区医学

Lipids Pub Date : 2026-07-15 DOI: 10.1002/lipd.70070

Mathilde Bertrand, Victor Simon, Lauriane Madec, Fabienne Le Grand, Arianna Servili, David Mazurais, Philippe Soudant, Marie Vagner

{"title":"A DHA-Deficient Diet Affects the n-3 LC PUFA Molecular Synthesis Pathway and Fatty Acid Composition in European Sardine (Sardina pilchardus).","authors":"Mathilde Bertrand, Victor Simon, Lauriane Madec, Fabienne Le Grand, Arianna Servili, David Mazurais, Philippe Soudant, Marie Vagner","doi":"10.1002/lipd.70070","DOIUrl":"https://doi.org/10.1002/lipd.70070","url":null,"abstract":"Climate change is expected to alter the phytoplankton production of n-3 long-chain polyunsaturated fatty acids (n-3 LC PUFA), including DHA (docosahexaenoic acid), impacting their transfer toward marine consumers. European sardine (Sardina pilchardus) is a major source of n-3 LC PUFA for human populations and is considered to be unable to synthesize sufficient quantities of n-3 LC PUFA to meet their physiological needs. This study aimed to understand the effect of a dietary DHA deficiency on the n-3 LC PUFA metabolism of sardine by investigating their fatty acid composition in three tissues (muscle, brain, and liver) and the hepatic expression of genes involved in the LC PUFA synthesis pathway. Wild-caught fish were fed either a DHA-deficient diet or a control diet for 2 months. The dietary DHA deficiency led to a lower DHA content in muscle reserves and liver membranes but did not change the brain fatty acid composition in sardines. In both dietary groups, the hepatic expressions of genes encoding elongase (elovl2, elovl5) and desaturase (fads2) enzymes were similar, but the expression levels of elovl2 and fads2 were negatively correlated to the DHA percentage in hepatic reserve lipids. The relative expressions of elovl5 and fads2 genes were positively correlated but only in DHA-deficient individuals. Besides, the individuals fed the DHA-deficient diet showed a higher expression of the phospholipase A2 gene (pla2g12b), suggesting a mobilization of n-3 LC PUFA from phospholipids. These results may suggest the activation of compensatory mechanisms at the molecular level in sardines to offset the dietary DHA deficiency.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammalian Phosphatidylcholine Specific Phospholipase C: Physiological Roles and Disease Implications. 哺乳动物磷脂酰胆碱特异性磷脂酶C：生理作用和疾病意义。

IF 1.6 4区医学

Lipids Pub Date : 2026-07-08 DOI: 10.1002/lipd.70073

Sabarirajan Jayaraja, Vasanthi Nachiappan, Balanehru Subramanian

{"title":"Mammalian Phosphatidylcholine Specific Phospholipase C: Physiological Roles and Disease Implications.","authors":"Sabarirajan Jayaraja, Vasanthi Nachiappan, Balanehru Subramanian","doi":"10.1002/lipd.70073","DOIUrl":"https://doi.org/10.1002/lipd.70073","url":null,"abstract":"Phosphatidylcholine-specific phospholipase C (PC-PLC) is a key lipid-metabolizing enzyme that breaks down phosphatidylcholine to form diacylglycerol (DAG) and phosphocholine. DAG, a key second messenger in diverse cellular signaling pathways, activates protein kinase C (PKC) and other downstream effectors. Phosphocholine, a biological product with possible biological significance, has no known direct signaling functions. These functions position PC-PLC as a central regulator of cell proliferation, differentiation, immune responses, and stress adaptation. Although bacterial PC-PLC has been structurally characterized and extensively studied as a model system, the mammalian PC-PLC gene remains unidentified, and its structural and isoform diversity are poorly understood. Recent evidence suggests functional redundancy with enzymes such as sphingomyelin synthases, highlighting the complexity of lipid network regulation. This review integrates current knowledge of PC-PLC biology across multiple organ systems, emphasizing its role in hepatic pathogen defense, cardiovascular remodeling, pulmonary barrier regulation, renal hemodynamics, reproductive signaling, neuroinflammation, and autoimmune disease. PC-PLC activity emerges as a molecular hub that links environmental and infectious stimuli to metabolic signaling, redox homeostasis, and disease progression, including cancer, fibrosis, and neurodegeneration. Despite these insights, key gaps remain regarding PC-PLC's molecular identity, regulatory mechanisms, and pharmacological tractability. We outline future research priorities, including cloning and structural characterization of mammalian PC-PLC, development of isoform-selective modulators, and systems-level lipidomics to map compensatory pathways. Targeting PC-PLC and its signaling network holds promise for novel therapeutic strategies in inflammatory, metabolic, and degenerative disorders.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photooxidation of 18-Carbon Polyunsaturated Fatty Acids to Prepare Mono-Hydroxylated Octadecanoids 18碳多不饱和脂肪酸光氧化制备单羟基十八烷酸。

IF 1.6 4区医学

Lipids Pub Date : 2026-07-07 Epub Date: 2026-02-17 DOI: 10.1002/lipd.70040

Johanna Revol-Cavalier, Mats Hamberg, Craig E. Wheelock

{"title":"Photooxidation of 18-Carbon Polyunsaturated Fatty Acids to Prepare Mono-Hydroxylated Octadecanoids","authors":"Johanna Revol-Cavalier, Mats Hamberg, Craig E. Wheelock","doi":"10.1002/lipd.70040","DOIUrl":"10.1002/lipd.70040","url":null,"abstract":"Oxylipins are oxygenated products of fatty acids that are being increasingly studied due to their role in multiple physiological processes. Investigations to date have focused on the 20-carbon eicosanoids and 22-carbon docosanoids. However, more recently, interest has grown into the 18-carbon octadecanoids. A significant obstacle in the study of these compounds is a lack of authentic standards for functional studies as well as for development of methods for their quantification. We developed a fast and simple one-step synthetic strategy to produce mono-hydroxylated metabolites based on the photosensitized oxidation of 18-carbon polyunsaturated fatty acids (PUFAs). Four different PUFAs including α-linolenic acid (ALA, ω3), γ-linolenic acid (GLA, ω6), stearidonic acid (SDA, ω3), and octadecapentaenoic acid (ODPA, ω3) were photooxidized in the presence of methylene blue and oxygen under exposure to light from a halogen lamp. The uncommon PUFA ODPA was prepared from docosahexaenoic acid (DHA, ω3) in a 6-step synthesis with 30% overall yield. The hydroperoxide products were reduced with sodium borohydride and the mono-hydroxylated octadecanoids were separated by HPLC. Product identification was performed by GC–MS. The final purities of isolated products ranged from 80% to 98%, with oxidation of non-terminal double bonds being preferred. It is likely that this approach could be extended to PUFAs of varying chain length, suggesting that photosensitized oxidation could be employed to rapidly prepare hydroperoxides from multiple unsaturated fatty acids. As interest in oxylipins continues to increase, this approach will be useful for large-scale preparation of multiple standards for the study of these new compounds.","PeriodicalId":18086,"journal":{"name":"Lipids","volume":"61 4","pages":"447-458"},"PeriodicalIF":1.6,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lipd.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipids最新文献