Laboratory Animal Research最新文献

{"title":"Sepsis as a confounding factor in assessing liver dysfunction in parenterally fed piglets: a model for human infants with intestinal failure.","authors":"Mahabub Alam, Pamela R Wizzard, Patrick N Nation, Michael Zaugg, Paul W Wales, Justine M Turner","doi":"10.1186/s42826-026-00279-5","DOIUrl":"https://doi.org/10.1186/s42826-026-00279-5","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a common complication of parenteral nutrition therapy for infants with intestinal failure, due to the presence of a central venous catheter and impairment of gut barrier function. Intestinal failure-associated liver disease (IFALD) is another complication, often attributed to the type of intravenous lipid emulsion delivered and this is commonly studied in pre-clinical animal models. Animal studies frequently overlook sepsis as a confounding factor, potentially biasing the results. We retrospectively reviewed 14-day studies of total parenteral nutrition (TPN) in piglets in our laboratory from 2011 to 2025, to determine if sepsis is an independent predictor of key liver outcomes relevant to IFALD research. Sepsis was defined as positive blood culture.</p><p><strong>Results: </strong>A total of 86 piglets (76 male; aged 2-5 days) were eligible for inclusion across four experimental studies. In total, 44 (51%) were suspected to have sepsis on the basis of clinical signs, 4 had missing blood cultures, and of these 28/40 (70%) had a positive blood culture. Septic piglets had higher mortality compared to those not suspected to be septic or having a negative blood culture (43% vs. 9%, p < 0.001). Septic piglets showed significantly lower bile flow (p < 0.001) and higher serum total bilirubin (p < 0.001) compared to non-septic piglets. The risk of developing sepsis was reduced in piglets with older age (OR = 0.42, p = 0.007) and increased when given a pure soy-oil compared to pure fish-oil emulsion (OR = 10.77, p = 0.05). Multivariate analysis showed the independent predictors of bile flow were both sepsis (negative) and higher body weight at study entry (positive) (R² = 0.42, p < 0.001), while sepsis and use of a soy-based lipid emulsion were independent positive predictors of total bilirubin (R² = 0.34, p < 0.001).</p><p><strong>Conclusions: </strong>Sepsis is associated with reduced bile flow, elevated total bilirubin and increased mortality in PN fed neonatal piglets. As sepsis can be a confounding factor in liver outcomes, researchers should both introduce refinements to mitigate sepsis as well as consistently report on the number of animals with sepsis within treatment groups and their outcomes.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-talk of gut-bone-muscle: osteosarcopenia in experimental colitis models.","authors":"Shilpa Sharma","doi":"10.1186/s42826-026-00273-x","DOIUrl":"https://doi.org/10.1186/s42826-026-00273-x","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracerebroventricular streptozotocin-induced animal model of Alzheimer's disease: revealing dose optimization, administration regimen, and molecular pathways.","authors":"Sagar A More, Radhika N Mundke, Yogeeta O Agrawal, Sanjay N Awathale, Sameer N Goyal, Kartik T Nakhate, Sumit S Rathod","doi":"10.1186/s42826-026-00278-6","DOIUrl":"10.1186/s42826-026-00278-6","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term effect of ovary removal on the joints of aged mice.","authors":"Sofia Wüstenhagen, Lindsay Zentveld, Francesco Longo, Loise Råberg, Alexandra Stubelius, Riddhi Vyas, Anders Nguyen, Julia M Scheffler, Mattias N D Svensson, Carmen Corciulo","doi":"10.1186/s42826-026-00271-z","DOIUrl":"10.1186/s42826-026-00271-z","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility and transcriptional characteristics of inbred C57BL/6J and outbred ICR mice in CDAHFD-induced MASH models.","authors":"Jing Zhou, Bingyi Li, Ziyi Guo, Bing Zhou, Yan Lu, Lian Zhu, Jin Lu, Jian Wang, E Wang, Yao Li","doi":"10.1186/s42826-026-00272-y","DOIUrl":"10.1186/s42826-026-00272-y","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel blood collection technique from the rat thoracic aorta: employing a diaphragm sac as a reservoir.","authors":"Purvaj G Barote, Abdulla Sherikar, Sameer N Goyal, Sanjay N Awathale","doi":"10.1186/s42826-026-00277-7","DOIUrl":"10.1186/s42826-026-00277-7","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel hairless highly immunodeficient mice model optimized for in vivo imaging.","authors":"Kouki Matsuda, Ryusho Kariya, Sawako Fujikawa, Kenji Maeda, Seiji Okada","doi":"10.1186/s42826-026-00275-9","DOIUrl":"10.1186/s42826-026-00275-9","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13054980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endovascular coil embolization compared to surgical ligation of the uterine artery in a non-human primate model in a model of preeclampsia.","authors":"A Makris, N Roshan, J Iliopoulos, R Waugh, A Al-Hindawi, B Corbett, C C Liu, B Lian, S Heffernan, J Hyett, J Ardui, S Pears, S A Karumanchi, A Hennessy","doi":"10.1186/s42826-026-00276-8","DOIUrl":"10.1186/s42826-026-00276-8","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia is a multisystem pregnancy disorder that is a major contributor to maternal and neonatal morbidity and mortality worldwide. An animal model facilitates further understanding of the disorder and allows for the investigation of targeted therapies prior to first in-human studies. Although there are several animal models for studying preeclampsia, the utero-placental ischemia (UPI) model is thought to best replicate the placental ischemia that develops as preeclampsia evolves in humans. An established non-human primate (NHP) model of UPI resembles human preeclampsia and has been important in understanding and finding cures for this disorder. To date the ischemia in most animal models has been undertaken by surgical ligation of a uterine artery. There are however alternate means to reduce arterial blood flow via endovascular means. This study aimed to investigate the difference between the surgical reduction in flow (surgical UPI) to the use of percutaneously delivered intra-arterial coils (coils UPI).</p><p><strong>Results: </strong>There was no significant difference between the two methods of inducing UPI for the measured parameters: circulating soluble fms-like tyrosine kinase-1(sFLT-1), blood pressure (BP) and proteinuria (p < 0.05). The results showed a percentage change from baseline in sFLT-1 of 3176 ± 1558% for the embolization and 2040 ± 1645% for the ligation group p = 0.11, three days post-UPI induction. Moreover, there was no difference between the two models in terms of preeclampsia-defining features.</p><p><strong>Conclusions: </strong>No statistically significant difference was found between the two methods, coils for endovascular embolization and surgical UPI. However, since endovascular embolization is a less invasive technique compared to ligation it may be preferred for induction of UPI in the NHP model.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13054988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic kidney disease induces a distinct lipidomic signature and accelerates atherosclerosis progression in a novel minipig model.","authors":"Marcelino Bermúdez-López, Paula Nogales, Manuel Martí-Antonio, Eva Castro-Boqué, Virtudes M de Lamo, Laia Beà-Menchón, Sergio Luis-Lima, Esteban Porrini, Xavier Sanchez-Salguero, Mariona Jové, Elia Obis, Natàlia Mota-Martorell, Aurora Pérez-Gómez, Alicia Garcia-Carrasco, Milica Bozic, Jesús Guajardo, Carlos J Pérez-Sánchez, Serafí Cambray, Núria Amigó, Reinald Pamplona, Jacob F Bentzon, José M Valdivielso","doi":"10.1186/s42826-026-00274-w","DOIUrl":"10.1186/s42826-026-00274-w","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular phylogeny and morphometric divergence of native Korean wild mice (Mus musculus).","authors":"Daewoo Kim, Jooseong Oh, Jang Geun Oh, Hee-Young Yang, Geun-Joong Kim, Tae-Hoon Lee, Bae-Keun Lee, Chungoo Park, Dong-Ha Nam","doi":"10.1186/s42826-026-00269-7","DOIUrl":"10.1186/s42826-026-00269-7","url":null,"abstract":"<p><strong>Background: </strong>The taxonomic status of house mice (Mus musculus) on the Korean Peninsula has long been debated due to conflicting morphological classifications and limited genetic evidence. Historically, three subspecies (M. m. molossinus, M. m. utsuryonis, and M. m. yamashinai) have been proposed based on external traits, although the validity of these proposals remains uncertain. Thus, this study aimed to integrate genetic and morphological analyses to clarify the phylogenetic relationships of Korean mice relative to the well-known primary M. musculus subspecies and evaluate the taxonomic distinctiveness.</p><p><strong>Results: </strong>Genetic analysis of mitochondrial DNA (cytb gene) from mice across Korea, including islands, mountains, and agricultural fields, confirmed that these mice belong to the Eurasian M. m. musculus lineage. Morphologically, Korean mice exhibited tail ratios consistent with previously assigned subspecies, suggesting these traits represent intraspecific variation within M. m. musculus. Craniometric analyses revealed distinctive features, such as a shorter, narrower premaxillary tooth-patch width and a longer maxillary tooth-row length, thereby distinguishing these mice from laboratory strains derived from M. m. domesticus. These cranial configurations, visualized via three-dimensional micro-computed tomography scans, further supported the morphological divergence of these mice from other subspecies.</p><p><strong>Conclusions: </strong>Our findings indicate that Korean house mice belong to a single subspecific group within M. m. musculus, with observed morphological variations reflecting local adaptation rather than distinct taxonomic divisions. The Korean Peninsula likely served as an ecological bridge, facilitating the spatiotemporal diversification of M. m. musculus across East Eurasia. This study resolves longstanding taxonomic ambiguities and underscores the subspecific status of Korean house mice within M. m. musculus. These insights provide a foundation for understanding the biogeographic history of human commensal species and future biomedical research utilizing wild-derived mouse models.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13015121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}