Evaluation of the protective effects of Spirulina platensis against cyclophosphamide-induced genotoxicity.

IF 2.7 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Laboratory Animal Research Pub Date : 2025-04-22 DOI:10.1186/s42826-025-00242-w

Kouamé Ephrem Zikpi, Aku Enam Motto, Kokou Atchou, Kounouho R Adounkpe Kougblenou, Povi Lawson-Evi, Kwashie Eklu-Gadegbeku

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Abstract

Background: Damage to normal cells is the most common limitation of cancer chemotherapy. Cyclophosphamide, one of the most widely used anticancer drugs due to its cytotoxicity, can bind to deoxyribonucleic acid (DNA), causing chromosomal breaks, micronuclei, and cell death. The use of natural sources helps to prevent this damage, and Spirulina platensis is highly appreciated for its numerous bioactive compounds. This study aimed to investigate the antigenotoxic effects of Spirulina platensis powder (PoSP) on mouse bone marrow cells in vivo via a micronucleus assay.

Results: Compared to the positive control, the administration of powder significantly reduced the PCE/PCE + NCE (polychromatic erythrocytes, normochromatic erythrocytes) ratio in treated mice. A significant increase in the percentage of MnPCE (micronucleus in polychromatic erythrocytes) in cyclophosphamide-treated bone marrow cells was observed. Compared with the positive controls, the groups treated with different doses in combination with cyclophosphamide presented a significant (p<0.0001) decrease in MnPCE in a dose-dependent manner. Compared to the positive control, PoSP significantly decreased MDA (malondialdehyde) levels in the livers of treated animals. The same things were observed in the kidneys and spleen. The catalase activity was also significantly increased in tissues, compared to negative control.

Conclusions: These findings suggest that PoSP does not cause DNA damage and can prevent genotoxicity, probably through its antioxidant activities.

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螺旋藻对环磷酰胺遗传毒性的保护作用评价。

背景：对正常细胞的损伤是癌症化疗最常见的局限性。环磷酰胺因其细胞毒性而成为应用最广泛的抗癌药物之一，可与脱氧核糖核酸（DNA）结合，导致染色体断裂、微核和细胞死亡。使用天然来源有助于防止这种损害，而螺旋藻因其众多的生物活性化合物而受到高度赞赏。本实验旨在通过微核实验研究螺旋藻粉（PoSP）对小鼠骨髓细胞的抗基因毒性作用。结果：与阳性对照组相比，给药散可显著降低小鼠PCE/PCE + NCE（多染红细胞、正染红细胞）比值。观察到环磷酰胺处理的骨髓细胞中MnPCE（多染红细胞微核）的百分比显著增加。与阳性对照组相比，不同剂量的环磷酰胺联合用药组均有显著性差异（p < 0.05）。结论：PoSP不引起DNA损伤，可能通过其抗氧化作用来预防遗传毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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