Laboratory Animal Research最新文献

{"title":"Increases in GFAP immunoreactive astrocytes in the cerebellar molecular layer of young adult CBA/J mice.","authors":"Cheryl Tyszkiewicz,&nbsp;Ingrid D Pardo,&nbsp;Hayley N Ritenour,&nbsp;Chang-Ning Liu,&nbsp;Chris Somps","doi":"10.1186/s42826-021-00100-5","DOIUrl":"https://doi.org/10.1186/s42826-021-00100-5","url":null,"abstract":"<p><strong>Background: </strong>CBA/J mice are standard experimental animals in auditory studies, and age-related changes in auditory pathways are well documented. However, changes in locomotion-related brain regions have not been systematically explored.</p><p><strong>Results: </strong>We showed an increase in immunoreactivity for glial fibrillary acidic protein (GFAP) in the cerebellar molecular layer associated with Purkinje cells in mice at 24 weeks of age but not in the younger mice. Increased GFAP immunoreactivity appeared in the form of clusters and distributed multifocally consistent with hyperplasia of astrocytes that were occasionally associated with Purkinje cell degeneration. Three out of 12 animals at 16 and 24 weeks of age exhibited pre-convulsive clinical signs. Two of these 3 animals also showed increased GFAP immunoreactivity in the cerebellum. Rotarod behavioral assessments indicated decreased performance at 24 weeks of age.</p><p><strong>Conclusions: </strong>These results suggest minimal to mild reactive astrocytosis likely associated with Purkinje cell degeneration in the cerebellum at 24 weeks of age in CBA/J mice. These findings should be taken into consideration prior to using this mouse strain for studying neuroinflammation or aging.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"24"},"PeriodicalIF":2.9,"publicationDate":"2021-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39362321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental animal models for diabetes and its related complications-a review.","authors":"Chidhambara Priya Dharshini Kottaisamy,&nbsp;Divya S Raj,&nbsp;V Prasanth Kumar,&nbsp;Umamaheswari Sankaran","doi":"10.1186/s42826-021-00101-4","DOIUrl":"https://doi.org/10.1186/s42826-021-00101-4","url":null,"abstract":"<p><p>Diabetes mellitus, a very common and multifaceted metabolic disorder is considered as one of the fastest growing public health problems in the world. It is characterized by hyperglycemia, a condition with high glucose level in the blood plasma resulting from defects in insulin secretion or its action and in some cases both the impairment in secretion and also action of insulin coexist. Historically, animal models have played a critical role in exploring and describing malady pathophysiology and recognizable proof of targets and surveying new remedial specialists and in vivo medicines. In the present study, we reviewed the experimental models employed for diabetes and for its related complications. This paper reviews briefly the broad chemical induction of alloxan and streptozotocin and its mechanisms associated with type 1 and type 2 diabetes. Also we highlighted the different models in other species and other animals.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"23"},"PeriodicalIF":2.9,"publicationDate":"2021-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39340864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Lipopolysaccharides of Fusobacterium nucleatum and Porphyromonas gingivalis increase RANKL-expressing neutrophils in air pouches of mice.","authors":"Ae Ri Kim,&nbsp;Yun Kyong Lim,&nbsp;Joong-Ki Kook,&nbsp;Eun-Jung Bak,&nbsp;Yun-Jung Yoo","doi":"10.1186/s42826-021-00099-9","DOIUrl":"https://doi.org/10.1186/s42826-021-00099-9","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"22"},"PeriodicalIF":2.9,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39296303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of intrinsic exercise capacity and response to acute exercise in ICR (Institute of Cancer Research) mice derived from three different lineages.","authors":"Dong-Joo Hwang,&nbsp;Ki-Chun Kwon,&nbsp;Dong-Hun Choi,&nbsp;Hyun-Keun Song,&nbsp;Kil-Soo Kim,&nbsp;Young-Suk Jung,&nbsp;Dae-Youn Hwang,&nbsp;Joon-Yong Cho","doi":"10.1186/s42826-021-00094-0","DOIUrl":"https://doi.org/10.1186/s42826-021-00094-0","url":null,"abstract":"<p><strong>Background: </strong>As a laboratory animal resource, the ICR mouse is commonly used in a variety of research fields. However, information on differences in exercise-related characteristics in ICR mice derived from different lineages and the underlying mechanisms remains to be elucidated. In this study, we investigated the intrinsic exercise capacity and a magnitude of response to acute exercise, and sought to identify mechanisms contributing to difference in Korl:ICR (a novel ICR lineage recently established by the National Institute of Food and Drug Safety Evaluation, Korea) and two commercialized ICR lineages derived from different origins (viz., A:ICR mouse from Orient Bio Com, the United States, and B:ICR mouse from Japan SLC Inc., Japan).</p><p><strong>Results: </strong>Results showed that despite no significant difference in body weight and weight-proportioned tissue mass of heart and skeletal muscles among groups, the relatively low intrinsic exercise capacity and exaggerated response to acute exercise were identified in B:ICR comparted with Korl:ICR and A:ICR, as reflected by total work and lactate threshold (LT). Also, the mitochondrial efficiency expressed as the complex 1 and complex 1 + 2 respiratory control ratio (RCR) values for cardiac mitochondrial O₂ consumption in B:ICR was significantly lower than that in Korl:ICR with higher level of state 2 respiration by glutamate/malate and UCP3 expression in cardiac muscle.</p><p><strong>Conclusions: </strong>Taken together, these results indicate that the intrinsic exercise capacity of ICR mouse varies according to lineages, suggesting the role of cardiac mitochondrial coupling efficiency as a possible mechanism that might contribute to differences in the intrinsic exercise capacity and magnitude of response to exercise.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"21"},"PeriodicalIF":2.9,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39276129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of particulate matter-induced lung injury model in mouse.","authors":"Se Yong Park,&nbsp;Kyu Sup An,&nbsp;Buhyun Lee,&nbsp;Ju-Hee Kang,&nbsp;Hyun Jin Jung,&nbsp;Min Woo Kim,&nbsp;Hyeon Yeol Ryu,&nbsp;Kyu-Suk Shim,&nbsp;Ki Taek Nam,&nbsp;Yeo Sung Yoon,&nbsp;Seung Hyun Oh","doi":"10.1186/s42826-021-00097-x","DOIUrl":"https://doi.org/10.1186/s42826-021-00097-x","url":null,"abstract":"<p><strong>Background: </strong>Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung diseases caused by PM. However, the induced severity of lung injury in animal models using PM varies from study to study due to disparities in the preparation of PM, and the route and number of PM administrations. In this study, we established an in vivo model to evaluate PM-induced lung injury in mice.</p><p><strong>Results: </strong>PM dispersion was prepared using SRM2975. Reactive oxygen species were increased in MLE 12 cells exposed to this PM dispersion. In vivo studies were conducted in the PM single challenge model, PM multiple challenge model, and PM challenge with ovalbumin-induced asthma using the PM dispersion. No histopathological changes were observed in lung tissues after a single injection of PM, whereas mild to moderate lung inflammation was obtained in the lungs of mice exposed to PM three times. However, fibrotic changes were barely seen, even though transmission electron microscopy (TEM) studies revealed the presence of PM particles in the alveolar macrophages and alveolar capillaries. In the OVA-PM model, peribronchial inflammation and mucous hypersecretion were more severe in the OVA+PM group than the OVA group. Serum IgE levels tended to increase in OVA+PM group than in OVA group.</p><p><strong>Conclusions: </strong>In this study, we established a PM-induced lung injury model to examine the lung damage induced by PM. Based on our results, repeated exposures of PM are necessary to induce lung inflammation by PM alone. PM challenge, in the presence of underlying diseases such as asthma, can also be an appropriate model for studying the health effect of PM.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"20"},"PeriodicalIF":2.9,"publicationDate":"2021-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42826-021-00097-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39261055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of colorectal carcinogenesis in the C57BL/6J and A/J mouse strains with a reduced DSS dose in the AOM/DSS model.","authors":"Henriette Arnesen,&nbsp;Mette Helen Bjørge Müller,&nbsp;Mona Aleksandersen,&nbsp;Gunn Charlotte Østby,&nbsp;Harald Carlsen,&nbsp;Jan Erik Paulsen,&nbsp;Preben Boysen","doi":"10.1186/s42826-021-00096-y","DOIUrl":"https://doi.org/10.1186/s42826-021-00096-y","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is one of the most frequently diagnosed cancers worldwide and thus mouse models of CRC are of significant value to study the pathogenesis. The Azoxymethane/Dextran sulfate sodium (AOM/DSS) model is a widely used, robust initiation-promotion model for chemical induction of colitis-associated CRC in rodents. However, the dosage of chemicals, treatment regimens and outcome measures vary greatly among studies employing this model. Thus, the aim of this study was to examine an AOM/DSS model involving a reduced (1%) dose of DSS for induction of carcinogenesis in A/J and C57BL/6J (B6) mice.</p><p><strong>Results: </strong>We show that colonic preneoplastic lesions can be reliably detected in A/J and B6 mice by use of a AOM/DSS model involving a single injection of 10 mg/kg AOM followed by three 7-day cycles of a low-dose (1%) DSS administration. Supporting existing evidence of A/J mice exhibiting higher susceptibility to AOM than B6 mice, our AOM/DSS-treated A/J mice developed the highest number of large colonic lesions. Clinical symptoms in both strains subjected to the AOM/DSS treatment did not persist in-between treatment cycles, demonstrating that the animals tolerated the treatment well.</p><p><strong>Conclusions: </strong>Our findings suggest that a reduced dose of DSS in the AOM/DSS model can be considered in future studies of early phase colorectal carcinogenesis in the A/J and B6 mouse strains using preneoplastic lesions as an outcome measure, and that such regimen may reduce the risk of early trial terminations to accommodate human endpoints. Overall, our data emphasize the importance of devoting attention towards choice of protocol, outcome measures and mouse strain in studies of CRC in mice according to the study purpose.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"19"},"PeriodicalIF":2.9,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42826-021-00096-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39232903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Working with laboratory rodents in Spain: a survey on welfare and wellbeing.","authors":"Olatz Goñi-Balentziaga,&nbsp;Iván Ortega-Saez,&nbsp;Sergi Vila,&nbsp;Garikoitz Azkona","doi":"10.1186/s42826-021-00098-w","DOIUrl":"https://doi.org/10.1186/s42826-021-00098-w","url":null,"abstract":"<p><strong>Background: </strong>Replacement, reduction and refinement, the 3R principles, provide a framework to minimize the use and suffering of animals in science. In this context, we aimed to determine the actual perception that individuals working with laboratory rodents in biomedical research have on animal welfare and on their interaction with the animals, as well as how they perceive its impact on their social relations. To this end, we designed an anonymous on-line survey for people working with rodents, at three responsibility levels, in Spain.</p><p><strong>Results: </strong>Of the 356 participants, 239 were women (67 %); 263 were researchers (74 %), and 93 animal facility staff (26 %), of which 55 were caretakers/technicians (15 %), and 38 welfare officer/veterinarians (11 %). Animal facility staff indicated environmental enrichment to be a universal practice. About half of the participants reported that, in their opinion, animals suffer \"little to none\" or \"minor\" stress and pain. Animal caretakers/technicians and researchers perceived higher levels of stress and pain than welfare officers/veterinarians. Participants judged decapitation the most unpleasant method to kill rodents, whereas anaesthetic overdose was the least one. A sizable proportion - 21 % of animal caretakers/technicians and 11.4 % of researchers - stated that they were never given the choice not to euthanize the rodents they work with. Overall, women reported higher interactions with animals than men. Nevertheless, we could detect a significant correlation between time spent with the animals and interaction scores. Notably, 80 % of animal facility staff and 92 % of researchers rarely talked about their work with laboratory rodents with people outside their inner social circle.</p><p><strong>Conclusions: </strong>Overall, the participants showed high awareness and sensitivity to rodent wellbeing; animal facility staff reported a similar perception on welfare questions, independently of their category, while researchers, who spent less time with the animals, showed less awareness and manifested lower human-animal interaction and less social support. Regarding the perception on social acceptance of laboratory animal work, all groups were cautious and rarely talked about their job, suggesting that it is considered a sensitive issue in Spain.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"18"},"PeriodicalIF":2.9,"publicationDate":"2021-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39227427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute and 28-days subacute toxicity studies of Gαq-RGS2 signaling inhibitor.","authors":"Jayesh V Beladiya,&nbsp;Anita A Mehta","doi":"10.1186/s42826-021-00093-1","DOIUrl":"https://doi.org/10.1186/s42826-021-00093-1","url":null,"abstract":"<p><strong>Background: </strong>The aim of study was to evaluate the single oral dose and 28 day repeated oral administration toxicity profile of the synthetic compound Gαq-RGS2 signaling inhibitor, (1-(5-chloro-2-hydroxyphenyl)-3-(4-(trifluoromethyl)phenyl)-1 H-1,2,4-triazol-5(4 H)-one) as per OECD guideline 425 (2008a) and 407 (2008b), respectively.</p><p><strong>Results: </strong>In acute toxicity study, a single oral dose administration of Gαq-RGS2 signaling inhibitor did not show any mortality at doses of 5, 50, 300 and 2000 mg/kg within 24 h and 14 days. The treatment of Gαq-RGS2 signaling inhibitor at dose 10 and 100 mg/kg for 28 days did not show any mortality, significant changes in the increase of body weight, various organ damage markers, hematological parameters, relative organ/body weight ratio and microscopic anatomical texture of essential organs as compared to vehicle and normal control.</p><p><strong>Conclusions: </strong>A single oral administration of Gαq-RGS2 signaling inhibitor up to dose of 2000 mg/kg in mice and repeated administration of Gαq-RGS2 signaling inhibitor at higher dose 100 mg/kg for 28 days in the rats is safe.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"17"},"PeriodicalIF":2.9,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42826-021-00093-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39224893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypothermic treatment reduces matrix metalloproteinase-9 expression and damage in the liver following asphyxial cardiac arrest in rats.","authors":"Donghwi Kim,&nbsp;Bora Kim,&nbsp;Hyejin Sim,&nbsp;Tae-Kyeong Lee,&nbsp;Hyun-Jin Tae,&nbsp;Jae-Chul Lee,&nbsp;Joon Ha Park,&nbsp;Jun Hwi Cho,&nbsp;Moo-Ho Won,&nbsp;Yoonsoo Park,&nbsp;Ji Hyeon Ahn","doi":"10.1186/s42826-021-00095-z","DOIUrl":"https://doi.org/10.1186/s42826-021-00095-z","url":null,"abstract":"<p><strong>Background: </strong>Hypothermic treatment is known to protect organs against cardiac arrest (CA) and improves survival rate. However, few studies have evaluated the effects of hypothermia on CA-induced liver damages. This study was designed to analyzed the possible protective effects of hypothermia on the liver after asphyxial CA (ACA). Rats were randomly subjected to 5 min of ACA followed by return of spontaneous circulation (ROSC). Body temperature was controlled at 37 ± 0.5 °C (normothermia group) or 33 ± 0.5 °C (hypothermia group) for 4 h after ROSC. Liver tissues were extracted and examined at 6 h, 12 h, 1 day, and 2 days after ROSC.</p><p><strong>Results: </strong>The expression of infiltrated neutrophil marker CD11b and matrix metallopeptidase-9 (MMP9) was investigated via immunohistochemistry. Morphological damage was assessed via hematoxylin and eosin (H & E) staining. Hypothermic treatment improved the survival rate at 6 h, 12 h, 1 day, and 2 days after ACA. Based on immunohistochemical analysis, the expression of CD11b and MMP9 was significantly increased from 6 h after ACA in the normothermia group. However, the expressions of CD11b and MMP9 was significantly decreased in the hypothermia group compared with that of the normothermia group. In addition, in the results of H & E, sinusoidal dilatation and vacuolization were apparent after ACA; however, these ACA-induced structural changes were reduced by the 4 h-long hypothermia.</p><p><strong>Conclusions: </strong>In conclusion, hypothermic treatment for 4 h inhibited the increases in CD11b and MMP9 expression and reduced the morphological damages in the liver following ACA in rats. This study suggests that hypothermic treatment after ACA reduces liver damages by regulating the expression of CD11b and MMP9.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"16"},"PeriodicalIF":2.9,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42826-021-00095-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39184420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on intraocular pressure under various anesthetics in cynomolgus monkeys (Macaca fascicularis).","authors":"Hong-Soo Lee,&nbsp;Da-Hee Kim,&nbsp;Sung-Hwan Kim,&nbsp;Min-Sung Kang,&nbsp;Han Na Suh","doi":"10.1186/s42826-021-00092-2","DOIUrl":"https://doi.org/10.1186/s42826-021-00092-2","url":null,"abstract":"<p><strong>Background: </strong>Nonhuman primates (NHPs) are superior model for ocular research due to its morphological and physiological similarities with humans. Thus, the effect of four different anesthetic combinations [ketamine (10 mg/kg), ketamine + xylazine (7 + 0.6 mg/kg), zoletil (4 mg/kg), and zoletil + xylazine (4 + 0.2 mg/kg)] on intraocular pressure (IOP) was determined in cynomolgus monkeys.</p><p><strong>Results: </strong>The administration of ketamine + xylazine or zoletil + xylazine resulted in lower IOP compared to ketamine or zoletil alone. Moreover, the IOP in male monkeys was higher than in females. The difference between the right and left eye was not found.</p><p><strong>Conclusions: </strong>Anesthetics affected the IOP, and gender differences should be considered when measuring the IOP of nonhuman primates (NHPs).</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"37 1","pages":"15"},"PeriodicalIF":2.9,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42826-021-00092-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39116232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}