Lasers in Medical Science最新文献

{"title":"Effects of antimicrobial photodynamic therapy with zinc oxide and toluidine blue on Streptococcus mutans accumulation in polymethyl methacrylate resin used in orthodontic appliances.","authors":"Erfan Mortazavi Zadeh, Amirhossein Mirhashemi, Maryam Pourhajibagher, Mahsa Ghorbani, Hooman Shafaee, Behrad Tanbakuchi","doi":"10.1007/s10103-025-04560-2","DOIUrl":"10.1007/s10103-025-04560-2","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate the efficacy of antimicrobial photodynamic therapy (aPDT) using toluidine blue (TBO) activated by a diode laser and zinc oxide (ZnO) activated by light-emitting diode (LED) light in reducing bacterial accumulation on polymethyl methacrylate (PMMA) orthodontic appliances.</p><p><strong>Methods: </strong>This in-vitro study investigated the formation of Streptococcus mutans biofilm on 32 PMMA resin discs, divided into four groups. The first group consisted of acrylic discs containing ZnO exposed to 450 nm LED radiation; the second group included acrylic discs with TBO and 635 nm diode laser radiation; the third group was the positive control with chlorhexidine (CHX); and the fourth group served as the negative control. To evaluate biofilm formation, the discs were immersed in tubes containing a microbial suspension at a 0.5 McFarland concentration. Following incubation, the discs were exposed to light according to the type of photosensitizer. After washing with saline and sonication, bacterial colony counts were determined by serial dilution and culture in Brian Heart Infusion agar medium. One-way ANOVA was used to compare the colony-forming unit (CFU) counts, and post-hoc Tamhane's test was performed for pairwise comparisons, with statistical significance set at p < 0.05.</p><p><strong>Results: </strong>The negative control group exhibited the highest mean colony count (35.72 ± 3.35 CFU/mL), while the positive control group showed the lowest mean colony count (5.87 ± 0.92 CFU/mL). Both ZnO and TBO groups, when activated by their respective light sources, demonstrated significant antibacterial activity compared to the negative control (P < 0.001 for both). However, CHX outperformed both ZnO and TBO in reducing bacterial growth (P < 0.001 and P = 0.007, respectively). No statistically significant difference was observed between the antibacterial effects of ZnO and TBO (P = 0.280).</p><p><strong>Conclusion: </strong>This study suggests that aPDT using ZnO and TBO can be an effective adjunctive treatment for reducing bacterial accumulation on orthodontic appliances. While CHX remains the most effective treatment, the cost-effectiveness and ease of application of both CHX and ZnO make them viable options for future clinical trials. Further research is needed to optimize treatment protocols and explore the potential impact of these treatments on other microorganisms and material properties.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"360"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on \"photobiomodulation treatment in incomplete spinal cord injuries or peripheral nerve injuries: a comprehensive systematic review of randomized clinical trials\".","authors":"Leela Lakshmi Sai Srinivas Parasa, Bhaskar Kambhampati, Ranjana Sah","doi":"10.1007/s10103-025-04622-5","DOIUrl":"https://doi.org/10.1007/s10103-025-04622-5","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"361"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The regular use of finasteride before transurethral laser resection of the prostate helps reduce bleeding.","authors":"Ruoyu Wang, Xiaoyong Gong, Shuiyuan Chen, Tiehuan Shu, Guiqiao Xiang, Sen Yang","doi":"10.1007/s10103-025-04618-1","DOIUrl":"10.1007/s10103-025-04618-1","url":null,"abstract":"<p><p>To investigate the effect of preoperative administration of finasteride on intraoperative bleeding during transurethral laser resection of the prostate. We retrospectively analyzed 120 patients who underwent transurethral laser resection of the prostate. Patients were divided into two groups based on whether finasteride was used (n = 60) or not (n = 60). The following parameters were collected: age, height, weight and body mass index (BMI), international prostate symptom score (IPSS), quality of life (QoL) score, prostate-specific antigen (PSA), prostate volume, operation time, hospital stay, continuous bladder irrigation (CBI); catheterization duration, re-catheterization, preoperative and postoperative hemoglobin concentrations, preoperative and postoperative serum sodium concentrations, postoperative urinary tract infection (UTI) rate, hospitalization costs. We compared the two groups using Student's t test, Mann-Whitney U test and χ2 test for quantitative and categorical variables, respectively. A linear regression model was employed to identify predictive factors of transurethral laser resection of the prostate. Compared with the non-finasteride group, the finasteride group had a lower bleeding level (Δ Hemoglobin 2.5 g/L vs. 7 g/L, p = 0.025), and lower hospitalization costs (21.62 thousand Yuan vs. 23.11 thousand Yuan, p = 0.001). In multivariate analyses, the regular use of finasteride (r=-0.304, p = 0.001) was the independent predictive factor of intraoperative bleeding during transurethral laser resection of the prostate. The regular use of finasteride before transurethral laser resection of the prostate helps to reduce intraoperative bleeding level.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"357"},"PeriodicalIF":2.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of pediatric dentists' knowledge, attitudes, and practices regarding the use of lasers : Regarding the use of lasers.","authors":"Eda Arat Maden, Mine Tirnaksiz Müftüoğlu, Buğra Özen, Ceyhan Altun","doi":"10.1007/s10103-025-04610-9","DOIUrl":"10.1007/s10103-025-04610-9","url":null,"abstract":"<p><p>This study aims to evaluate pediatric dentists' knowledge, attitudes, and clinical practices regarding the use of laser technology. Additionally, it seeks to identify educational needs to support the accurate and effective implementation of laser applications in pediatric dentistry. The study received ethical approval from the Non-Interventional Research Ethics Committee of ………….University. Data were collected through an online questionnaire distributed via email and social media. A total of 233 volunteer pediatric dentists participated in the survey, which included 26 questions across five sections: sociodemographic data, knowledge, attitudes, clinical practices regarding laser use in pediatric dentistry, and laser-related training. Participation was voluntary, and informed consent was obtained at the beginning of the survey. The results of the study showed that 85.8% of pediatric dentists were knowledgeable about the use of lasers in pediatric dentistry. However, this knowledge was not fully reflected in clinical practice. It was found that only 35.2% of pediatric dentists had performed laser-assisted procedures. Among these, the most frequently performed procedures were frenectomy (73.2%) and the exposure of unerupted teeth (64.6%). Knowledge and attitude scores were significantly higher among dentists aged 41-50 and those with more than 20 years of professional experience (p < 0.05). 73.8% of the participants stated that laser education should be integrated into pediatric dentistry training programs.There are variations in pediatric dentists' knowledge and clinical practices regarding the use of lasers. To ensure the safe and effective use of this technology, educational opportunities need to be improved.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"356"},"PeriodicalIF":2.4,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of antimicrobial photodynamic therapy on micro-shear bond strength to dentin.","authors":"Christian Giancarlo Bernal Rodriguez, Maria Paula Novaes Camargo Manna, Fiorella Elizabeth Arévalo Tarrillo, Carlos de Paula Eduardo","doi":"10.1007/s10103-025-04602-9","DOIUrl":"https://doi.org/10.1007/s10103-025-04602-9","url":null,"abstract":"<p><p>To evaluate in vitro the bond strength through the micro-shear test of two adhesive systems (self-etching and total-etch), on bovine dentin surfaces after aPDT. For the aPDT, 0.01% methylene blue and two different light sources (red laser and LED) were used.Sixty samples were randomly divided into 6 groups (n = 10) for the micro-shear test: G1: self-etching adhesive (USB) without aPDT, G2: aPDT (laser) + USB, G3: aPDT (LED) + USB; G4: total-etch adhesive (ASB) without aPDT; G5: aPDT (laser) + ASB, G6: aPDT (LED) + ASB), followed by analysis of the fracture patterns of the samples. For statistical analysis, a two-way ANOVA (adhesive and treatment) and Tukey's test were performed, adopting a significance level of 5%.Regarding bond strength, there was no significant difference between the 6 groups analyzed. An adhesive fracture pattern predominated.Based on the results and the irradiation parameters used in this study, no changes in the bond strength of the resin to healthy dentin were observed after treatment with aPDT.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"355"},"PeriodicalIF":2.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic inactivation of bacterial biofilms on absorbable collagen membranes using indocyanine green in aqueous and hydrogen peroxide solutions.","authors":"Hesam Mohammad Zaman, Yashar Safaraei, Nasim Chiniforush, Shima Afrasiabi","doi":"10.1007/s10103-025-04605-6","DOIUrl":"https://doi.org/10.1007/s10103-025-04605-6","url":null,"abstract":"<p><p>Microbial contamination of absorbable collagen membranes used in guided bone regeneration (GBR) may compromise healing outcomes. This study aimed to investigate whether the minimum inhibitory concentration (MIC) of hydrogen peroxide (H₂O₂) can improve the antibacterial effect of indocyanine green (ICG)-mediated antimicrobial photodynamic therapy (PDT) on absorbable collagen membranes while reducing the need for high H₂O₂ concentrations. A laboratory-based model was developed using Streptococcus sanguinis and Staphylococcus aureus. The biofilms were treated with ICG (1.29 mM), H₂O₂ (at MIC), or their combination, followed by irradiation at 808 nm (12 J cm^-²) or no irradiation. The biofilm viability was assessed after treatment. PDT (ICG + 808 nm diode laser) showed a significant reduction of bacteria, which was further enhanced by the addition of H₂O₂ at MIC concentrations. Although ICG-H₂O₂-PDT showed similar efficacy to 1 M H₂O₂, this effect was dependent on light activation. Without irradiation, no comparable result was observed, emphasizing that photoactivation is essential for achieving synergistic effects with H₂O₂ at low concentration. This combination could effectively combat biofilms on collagen membranes and provide an alternative for infection control by reducing the dependence on high H₂O₂ concentrations.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"354"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-Aminolevulinic acid-mediated photodynamic therapy improves scar healing of laryngeal wounds in rats.","authors":"Jing Li, Yuanyuan Lu, Huiying Hu, Xiao Han, Yufei Pan, Kai Sun, Kai Liu, Zhenkun Yu","doi":"10.1007/s10103-025-04588-4","DOIUrl":"https://doi.org/10.1007/s10103-025-04588-4","url":null,"abstract":"<p><p>To evaluated the efficacy of photodynamic therapy (PDT) in improving laryngeal mucosal wound scar healing in vivo and investigated its underlying mechanisms. Laryngeal mucosal wounds were induced in Sprague-Dawley rats. Two weeks post-injury, PDT was administered via intraperitoneal injection of 100 mg/kg 5-aminolevulinic acid (5-ALA) and 635-nm red laser irradiation at varying energy doses (15, 30, and 45 J/cm²). The wounds' histological assessment was performed one month after two PDTs (one/week), including hematoxylin-eosin, Masson staining, Immunohistochemical staining for collagen I and III, and Alcian Blue staining for the detection of hyaluronic acid. In vitro, the viability of human skin fibroblasts (HFF-1) exposed to different light energy (0.3, 0.6, and 1.2 J/cm²) was assessed using CCK-8. Sublethal-dose PDT (SL-PDT; 5-ALA: 0.125 mmol/L, light: 0.6 J/cm²) was used in subsequent experiments. The effect of PDT on fibroblast differentiation into myofibroblasts was evaluated by measuring α-SMA expression in fibroblasts through RT-qPCR and Western blotting. Histological evaluation revealed that following PDT, the trauma site thickness increased, collagen density decreased, and hyaluronic acid deposition was elevated. Immunohistochemical analysis showed improved distribution of type I and type III collagen, coupled with a reduction in density. The most prominent change was observed in the 45 J/cm² PDT group. PDT had a dose-dependent effect on fibroblast viability, and significantly reduced fibroblast differentiation into myofibroblasts by upregulating urokinase plasminogen activator receptor (uPAR) protein expression in fibroblast. PDT can alleviate scar healing of laryngeal tissue wounds by suppressing excessive myofibroblast activation, effectively preserving laryngeal function.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"353"},"PeriodicalIF":2.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Laser versus rhomboid flap for sacrococcygeal pilonidal sinus: multi-centre comparative study.","authors":"Alaa Alwadees, Abdulzahra Hussain, Emad Geddoa, Ali Younus Altamimi, Tamer Habeeb, Sahl Jafer, Samer Al-Hakkak","doi":"10.1007/s10103-025-04607-4","DOIUrl":"https://doi.org/10.1007/s10103-025-04607-4","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"352"},"PeriodicalIF":2.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review and meta-analysis of the comparison between lasers and other therapeutic modalities in skin rejuvenation and resurfacing with a focus on RCTs.","authors":"Sogand Sodagar, Abbas Dehghani, Alireza Jafarzadeh, Farnoosh Seirafianpour, Nazila Ghoreishi Amin, Rohollah Valizadeh, Azadeh Goodarzi","doi":"10.1007/s10103-025-04604-7","DOIUrl":"10.1007/s10103-025-04604-7","url":null,"abstract":"<p><strong>Aims and objectives: </strong>This systematic review and meta-analysis sought to compare the efficacy of lasers with other modalities in skin rejuvenation and resurfacing. Methodology: The database was thoroughly searched, and six articles meeting specific inclusion criteria were selected for meta-analysis. Inclusion criteria comprised studies that compared lasers with other methods, with four studies focusing on laser comparisons and two studies comparing more than two methods. The selected articles encompassed two randomized split-face trials, one side-by-side randomized clinical trial, and three comparative studies. The effectiveness of treatments was categorized as excellent, good, fair, poor, no change, or worsening. Article review and data extraction were conducted using Endnote X8, followed by analysis and categorization of the studies.</p><p><strong>Results: </strong>The meta-analysis, involving six studies with 497 patients (mean age: 49.65 years), revealed an overall pooled prevalence of treatment effectiveness: 18% for \"excellent,\" 31% for \"good,\" 40% for \"fair,\" 26% for \"poor,\" and 14% for \"no change.\" Erbium YAG laser exhibited superior results in the \"excellent\" category, with 20% displaying excellent responsiveness, and in the \"good\" category, 39% of radiofrequency cases treated with Erbium YAG laser demonstrated favorable results. Conversely, intense pulsed light showed better responsiveness in the \"fair\" group, with 39% of cases demonstrating fair results.</p><p><strong>Conclusion: </strong>Our study indicated a higher percentage of excellent responses compared to other skin rejuvenation treatments with Erbium YAG laser and the highest percentage of good responses with radiofrequency treatment. However, intense pulsed light treatment yielded superior results in the fair response group. Based on our findings, it is evident that utilizing Erbium YAG laser in conjunction with radiofrequency represents a secure approach that surpasses other methods in enhancing and restoring skin quality. Furthermore, a plausible suggestion is made that a combination of these two methods may introduce the most optimal approach for skin rejuvenation in the future.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"351"},"PeriodicalIF":2.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of 1064 nm nd: YAG laser for hair removal in Sudanese women: A study on Fitzpatrick skin types IV-VI.","authors":"Eslam E A Mahmoud, Osman Abdelmalik Elbasheer, Ali A S Marouf","doi":"10.1007/s10103-025-04606-5","DOIUrl":"https://doi.org/10.1007/s10103-025-04606-5","url":null,"abstract":"<p><p>This study investigated the efficacy and safety of Nd: YAG laser (1064 nm) hair removal in 55 Sudanese women with Fitzpatrick skin types IV, V, and VI. The primary objective was to evaluate the influence of skin type, fluence, age, and geographic location on the number of treatments required to achieve satisfactory hair reduction in the chin area. Treatment parameters, including fluence (25-40 J/cm²), spot size (10-12 mm), and pulse width (20-40 ms), were tailored to individual characteristics using the Cynosure Elite + Nd: YAG laser with sapphire contact cooling. Outcomes were assessed using clinical evaluation, pre- and post-treatment photographs, and a standardized 5-point hair density scale. Statistical analysis revealed that fluence significantly impacted outcomes (p = 0.039), with 25 J/cm² demonstrating effectiveness. Skin type, age, and geographic location were not significant factors (p > 0.05). No topical bleaching cream was used, and many participants reported improvement in skin tone. No adverse events or paradoxical hypertrichosis were observed. The study supports the safe and effective use of low-fluence 1064 nm Nd: YAG laser treatment for hair removal in darker skin types. Future research should examine long-term outcomes and broader treatment sites.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"349"},"PeriodicalIF":2.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}