Photodynamic inactivation of bacterial biofilms on absorbable collagen membranes using indocyanine green in aqueous and hydrogen peroxide solutions.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL

Lasers in Medical Science Pub Date : 2025-09-10 DOI:10.1007/s10103-025-04605-6

Hesam Mohammad Zaman, Yashar Safaraei, Nasim Chiniforush, Shima Afrasiabi

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引用次数: 0

Abstract

Microbial contamination of absorbable collagen membranes used in guided bone regeneration (GBR) may compromise healing outcomes. This study aimed to investigate whether the minimum inhibitory concentration (MIC) of hydrogen peroxide (H₂O₂) can improve the antibacterial effect of indocyanine green (ICG)-mediated antimicrobial photodynamic therapy (PDT) on absorbable collagen membranes while reducing the need for high H₂O₂ concentrations. A laboratory-based model was developed using Streptococcus sanguinis and Staphylococcus aureus. The biofilms were treated with ICG (1.29 mM), H₂O₂ (at MIC), or their combination, followed by irradiation at 808 nm (12 J cm^-²) or no irradiation. The biofilm viability was assessed after treatment. PDT (ICG + 808 nm diode laser) showed a significant reduction of bacteria, which was further enhanced by the addition of H₂O₂ at MIC concentrations. Although ICG-H₂O₂-PDT showed similar efficacy to 1 M H₂O₂, this effect was dependent on light activation. Without irradiation, no comparable result was observed, emphasizing that photoactivation is essential for achieving synergistic effects with H₂O₂ at low concentration. This combination could effectively combat biofilms on collagen membranes and provide an alternative for infection control by reducing the dependence on high H₂O₂ concentrations.

查看原文本刊更多论文

在水和双氧水溶液中使用吲哚菁绿对可吸收胶原膜上细菌生物膜的光动力失活。

用于引导骨再生（GBR）的可吸收胶原膜的微生物污染可能会影响愈合结果。本研究旨在探讨过氧化氢（H2O2）的最低抑制浓度（MIC）是否可以提高吲哚菁绿（ICG）介导的抗菌光动力疗法（PDT）对可吸收胶原膜的抑菌效果，同时减少对高浓度H2O2的需求。使用血链球菌和金黄色葡萄球菌建立了实验室模型。用ICG （1.29 mM）、H2O2 （MIC）或两者联合处理生物膜，然后在808 nm （12 J cm-²）照射或不照射。处理后评估生物膜活力。PDT （ICG + 808 nm二极管激光）显示出细菌的显著减少，在MIC浓度下添加H2O2进一步增强了细菌的减少。虽然ICG-H2O2-PDT表现出与1 M H2O2相似的效果，但这种效果依赖于光激活。在没有照射的情况下，没有观察到类似的结果，强调光激活是实现与低浓度H2O2协同作用的必要条件。这种组合可以有效地对抗胶原膜上的生物膜，并通过减少对高浓度H2O2的依赖，为感染控制提供了另一种选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.