Lasers in Surgery and Medicine最新文献

{"title":"Differentiation of Healthy Ex Vivo Bovine Tissues Using Raman Spectroscopy and Interpretable Machine Learning.","authors":"Soha Yousuf, Mohamed Irfan Karukappadath, Azhar Zam","doi":"10.1002/lsm.70031","DOIUrl":"https://doi.org/10.1002/lsm.70031","url":null,"abstract":"<p><strong>Objectives: </strong>Integrating machine learning with Raman spectroscopy (RS) shows strong potential for intraoperative guidance in orthopedic procedures, but limited algorithm transparency remains a barrier to clinician trust. This study aims to develop interpretable machine learning models capable of accurately classifying bovine tissue types (bone, bone marrow, fat, and muscle) relevant to orthopedic surgery by identifying key Raman biomarkers to improve model transparency.</p><p><strong>Methods: </strong>A portable RS system equipped with a 785 nm fiber-optic probe was used to collect spectral data from excised bovine tissues, including bone, bone marrow, muscle, and fat. One-dimensional convolutional neural network (1D-CNN) and support vector machine (SVM) models were developed to classify these tissue types. The Raman spectral data were divided using a sample-based, stratified splitting strategy and evaluated across 30 independent iterations. Feature importance maps were generated for both models, and matching scores were calculated to correlate significant spectral features with known Raman biomarkers.</p><p><strong>Results: </strong>Through feature importance analysis and matching scores generated by the 1D-CNN and SVM models, critical Raman biomarkers-including hydroxyapatite, lipids, amino acids, and collagen-were identified as essential for distinguishing between the different bovine tissue types, providing deeper insights into their molecular differences.</p><p><strong>Conclusions: </strong>The integration of interpretable machine learning models with RS enabled accurate differentiation of bovine tissues relevant to orthopedic surgery, while enhancing model transparency through biomarker identification. Linking model predictions to biologically meaningful Raman features supports the development of RS as a reliable tool for precision-guided surgical procedures.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abstract","authors":"","doi":"10.1002/lsm.70014","DOIUrl":"https://doi.org/10.1002/lsm.70014","url":null,"abstract":"","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 S37","pages":"S5-S165"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image: Volume 57 Issue S37","authors":"","doi":"10.1002/lsm.70028","DOIUrl":"https://doi.org/10.1002/lsm.70028","url":null,"abstract":"<p>\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 S37","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Acquired Bilateral Nevus of Ota-Like Macules Patients' Clinical Characteristics and Melasma Induction/Exacerbation After Q-Switched Laser: A Decadal Comparative Study of 2675 Chinese Women in Two Different Periods.","authors":"Ying Li, Ziyang Huang, Yiran Peng, Yingxue Huang, Wei Shi, Ben Wang, Yaling Wang, Dan Jian","doi":"10.1002/lsm.70024","DOIUrl":"https://doi.org/10.1002/lsm.70024","url":null,"abstract":"<p><strong>Background: </strong>Post-laser melasma induction/exacerbation remains a critical challenge in treating acquired bilateral nevus of Ota-like macules (ABNOMs). While laser parameters are well-studied, patient-specific risk factors across evolving therapeutic eras require elucidation.</p><p><strong>Methods: </strong>This retrospective cohort analyzed 2675 ABNOMs patients treated with Q-switched lasers in two eras: C1 (2010-2013; 1064 nm-only, n = 1069) and C2 (2020-2023; multi-wavelength, n = 1606). Risk factors were assessed through multivariate logistic regression, incorporating laser wavelength stratification.</p><p><strong>Results: </strong>Era-specific risk reversals emerged: In C1, age > 25, Fitzpatrick IV and patch lesions predicted melasma induction, whereas large/dark lesions showed protective effects. C2 showed inverse risks for lesion characteristics (large/dark lesions showed negative effects), with skin type/age becoming nonsignificant. Despite increased number of patients with pre-existing melasma in C2, melasma exacerbation rates decreased from 87.6% (C1) to 50.1% (C2, p < 0.05) in parallel with diagnostic advances (subclinical melasma detection) and protocol optimization. Wavelength-specific analysis revealed a persistent Fitzpatrick IV risk with 1064 nm versus lesion-driven risks with 694/755 nm. Paradoxically, treatment of dark lesions with 1064 nm reduced the risk of melasma exacerbation, probably through selective melanin targeting.</p><p><strong>Conclusion: </strong>ABNOMs-related melasma risks dynamically evolve with diagnostic/therapeutic advancements, demanding phenotype-specific laser parameter selection. This decade-spanning analysis provides actionable insights for optimizing laser therapy in diverse ABNOMs populations, despite inherent retrospective limitations.</p><p><strong>Clinical trial registration: </strong>Registry and the Registration No. of the study/trial: ChiCTR2500095697.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"254 nm Ultraviolet C Light Emitting Diode (LED) Radiation Enhances Wound Healing in a Rat Model of Full-Thickness Skin Defects.","authors":"Wei Chen, Zhao Zhang, Xiao Zhang, Jing Zuo, Xiao Li, Shuang-Cheng Li, Yue-Ming Gao","doi":"10.1002/lsm.70030","DOIUrl":"https://doi.org/10.1002/lsm.70030","url":null,"abstract":"<p><strong>Significance: </strong>254 nm short wave ultraviolet (UVC) emitted by low-pressure mercury lamp is effective in the treatment of skin wounds. With the emergence of deep ultraviolet light-emitting diodes (LED), it is expected to significantly expand the clinical indications of UVC. However, whether 254 nm UVC generated by LED light source can promote skin wound healing has not been reported.</p><p><strong>Aim: </strong>To clarify the effect of 254 nm UVC-LED light source on promoting skin wound healing and explore the mechanism of UVC-LED promoting wound healing.</p><p><strong>Approach: </strong>The full-thickness wound model of rat back skin was constructed, and the skin wounds of rats were treated with low-pressure mercury lamp and 254 nm UVC-LED equipment respectively. The curative effect of UVC-LED was determined by wound healing rate and histopathological changes. The expression of transforming growth factor-alpha (TGF-α), epidermal growth factor receptor (EGFR), cluster of differentiation 31 (CD31), cluster of differentiation 68 (CD68), vascular endothelial growth factor (VEGF) and the mRNA content of TGF-α, EGFR, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and VEGF were detected to clarify the mechanism of UVC-LED promoting wound healing.</p><p><strong>Results: </strong>Histopathology showed that both low-pressure mercury lamp and UVC-LED irradiation could promote skin wound healing, reduce the degree of inflammatory response, and promote angiogenesis. UVC-LED could better promote collagen fiber proliferation. UVC-LED promotes the expression of key cytokines in wound re-epithelialization by increasing the levels of TGF-α and EGFR, dynamically regulates wound healing by bidirectionally regulating the expression of inflammatory factors TNF-α and IL-6, promotes wound angiogenesis by increasing the levels of CD31 and VEGF, and reduces and regulates inflammation by increasing the number of macrophages by increasing CD68.</p><p><strong>Conclusions: </strong>254 nm UVC-LED can significantly promote skin wound healing in rats and play a role by regulating tissue regeneration cytokines, inflammatory factors and angiogenesis mechanisms.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-Year Follow-Up of Acne Vulgaris Patients Treated With Nonablative Monopolar Radiofrequency: Efficacy and Safety Assessment.","authors":"Woraphong Manuskiatti, Supisara Wongdama, Jayne Bernadeth Li, Woramate Bhorntarakcharoen, Sariya Sittiwanaruk, Thanya Techapichetvanich, Kanokvalai Kulthanan","doi":"10.1002/lsm.70026","DOIUrl":"https://doi.org/10.1002/lsm.70026","url":null,"abstract":"<p><strong>Background: </strong>Moderate-to-severe acne vulgaris (AV) remains a therapeutic challenge, often demonstrating resistance to standard interventions, including topical agents, oral antibiotics, and systemic retinoids. Nonablative monopolar radiofrequency (NMRF) offers a promising alternative by delivering controlled dermal heating without epidermal disruption. Initial findings from the authors demonstrated significant reductions in inflammatory lesion counts and sebum production following two NMRF sessions administered 4 weeks apart, with results sustained up to 6 months. This updated study extends the evaluation to include additional clinical assessments at 9- and 12-month posttreatment, providing further insights into the long-term efficacy and safety of this modality.</p><p><strong>Objective: </strong>To evaluate the extended efficacy and safety of NMRF therapy in the management of moderate-to-severe AV, incorporating follow-up assessments at 9- and 12-month posttreatment.</p><p><strong>Methods: </strong>The study included 20 participants with moderate-to-severe AV who had been previously enrolled in the authors' initial investigation. All participants underwent two NMRF sessions, spaced 4 weeks apart, as per the original protocol. Follow-up assessments were conducted at 9- and 12-month posttreatment. Lesions were categorized as inflammatory (papules, pustules, nodules, cysts) or noninflammatory (comedones). Sebum production was measured quantitatively using a Sebumeter at each follow-up visit. Patient-reported outcomes, including perceived improvement and satisfaction, were also documented.</p><p><strong>Results: </strong>Of the initial 20 participants, 11 completed the 12-month follow-up. Clinical assessments revealed statistically significant reductions in both inflammatory and noninflammatory lesion counts, with the most substantial decreases observed in papules, pustules, nodules, and cysts. Sebum levels continued to decline consistently over the extended follow-up period. Patient satisfaction scores remained high at both time points, and no significant adverse effects were reported, reinforcing the favorable safety and tolerability profile of NMRF therapy.</p><p><strong>Conclusion: </strong>NMRF is a safe, effective, and minimally invasive treatment for moderate-to-severe AV. The 1-year follow-up data highlight its potential as a long-term treatment option, demonstrating sustained reductions in lesion counts and sebum production following a two-session course.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Erbium and CO₂ Genital Laser Treatment on Genitourinary Syndrome in Female Patients After Breast Cancer: A Scoping Review.","authors":"Keila S T Ferreira, Nathalia S Guimarães, Gisele V de Oliveira","doi":"10.1002/lsm.70027","DOIUrl":"https://doi.org/10.1002/lsm.70027","url":null,"abstract":"<p><strong>Objectives: </strong>This scoping review aimed to synthesize the existing scientific literature on the methods, tools, and strategies employed in vaginal laser therapy for treating genitourinary syndrome in breast cancer survivors.</p><p><strong>Methods: </strong>A systematic search was conducted in August 2024 across six electronic databases-MEDLINE (via PubMed), Embase (Elsevier), Scopus (Elsevier), Cochrane Library (Central), Virtual Health Library (VHL), and Web of Science (Clarivate Analytics)-for studies reporting the use of CO₂ or erbium lasers in breast cancer survivors. The search included articles in all languages and used MeSH terms and database-specific adaptations.</p><p><strong>Results: </strong>A total of 2372 studies were identified through the electronic databases. After excluding 458 duplicates, 2134 titles and abstracts were screened. Thirty full-text records were assessed for eligibility, of which 10 were excluded for incomplete results and different technologies. Ultimately, 1189 patients out of 20 studies were selected for inclusion in this review: 14 studies used CO₂ laser, 5 used erbium laser, and 1 used both technologies. This review showed that use of vaginal lasers to approach GSM in BSC patients is being used since 2017, and 13 out of 20 studies concluded for the safety and efficacy of vaginal laser to treat GSM in BCS patients, comparable to standard treatment.</p><p><strong>Conclusion: </strong>Both CO₂ and erbium lasers appear to be safe and effective treatment options for breast cancer survivors with genitourinary syndrome. New larger, multicenter studies are needed to enhance safety, standardize treatment protocols, and provide further evidence on the efficacy of these therapies in this patient population.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-Focused Ultrasound Technology for Remodeling of Skin Tissue Architecture in an Animal Model.","authors":"Huimiao Tang, Yue Li, Xi Xiang, Wenxin Li, Hao Wang, Michael R Hamblin, Xiang Wen","doi":"10.1002/lsm.70025","DOIUrl":"https://doi.org/10.1002/lsm.70025","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;As a novel clinical therapeutic technique, micro-focused ultrasound (MFU) has garnered significant attention in the field of skin rejuvenation in recent years. Although clinical studies have demonstrated the marked efficacy of MFU in improving skin laxity, the specific histological and molecular mechanisms of its rejuvenation effects on the skin remain unclear.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The abdominal skin of Bama minipigs was treated with MFU, specific treatment parameters employed both the 3 mm and 4.5 mm therapeutic head with the following settings: 25 Hz/1 min/6.63 W, 25 Hz/2.5 min/6.63 W, 25 Hz/1 min/1.32 W, and 10 Hz/1 min/6.63 W, with adjacent areas serving as controls. The thickness of the dermis, fat layer, and superficial musculoaponeurotic system (SMAS) layer was measured by ultrasound imaging at baseline, immediately after treatment, and at 14, 30, 90 days posttreatment. The histopathological, immunohistochemical, and transcriptomic changes of each time point were studied, to compare protein content and gene expression of matrix metalloproteinase (MMP) enzymes, transforming growth factor β (TGF-β), epidermal growth factor (EGF), Ki67, type I collagen, and elastin.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;MFU induced dermal thickening, which was sustained up to 90 days. Immediately posttreatment (0 days), the parameters 4.5 mm, 10 Hz, 1 min, 6.63 W showed an average thickening of 0.16 mm, with a growth rate of 14.48 ± 3.64%, which was significantly higher than the 0% growth rate observed in the control group (p &lt; 0.05). With the parameters of 3.0 mm, 25 Hz, 1 min, 1.32 W, the dermis thickened by an average of 0.69 mm at 90 days posttreatment, with a growth rate of 39.33 ± 14.34%. In contrast, the control group showed an increase of about 0.18 mm, with a growth rate of 0.92 ± 13.25% (p &lt; 0.05). A prolonged treatment duration (2.5 min) and higher power levels (6.63 W) did not exhibit statistically different effects on the promotion of dermal thickness in this study. MFU also promoted collagen production in the SMAS layer in the long term. After treatment with parameters 3.0 mm, 25 Hz, 1 min, 1.32 W, the SMAS thickened by 0.12 mm at 30 days, while the control group showed an increase of 0.03 mm (p &lt; 0.05). At 90 days, the SMAS thickened by approximately 0.17 mm, whereas the control group decreased by 0.04 mm (p &lt; 0.05). Following treatment with parameters 3.0 mm, 10 Hz, 1 min, 6.63 W, the SMAS thickened by 0.11 mm at 30 days, with the control group showing an increase of 0.06 mm (p &lt; 0.05). Immunofluorescence staining indicated a significant increase in type I and III collagen, MMP1, MMP3, TGF-β and Ki67 at 14 and 30 days after MFU treatment. The gene expression of MMP family members, TGF-β, EGF, type I collagen, and elastin fibers was markedly upregulated at 14, 30, and 90 days.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;MFU treatment can stimulate the proliferation of collagen fibers in the dermis. It can also promo","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Ex-Vivo Bond Quality Monitoring of Plasmonic Metal Oxide Biomaterials Mediated Laser Tissue Soldering Process Using Fast Noninvasive Optical Tomographic Imaging.","authors":"Sweta Satpathy, Abhishek Banerjee, Ishita Banerjee, Raju Poddar","doi":"10.1002/lsm.70023","DOIUrl":"https://doi.org/10.1002/lsm.70023","url":null,"abstract":"<p><strong>Background and objectives: </strong>Laser tissue soldering (LTS) offers an innovative, suture-free approach to wound closure. However, challenges such as limited tensile strength and prolonged soldering time need solutions. This work combines BSA with PEG to enhance mechanical properties and introduces silver and titanium dioxide nanoparticles to accelerate soldering via localized surface plasmon resonance (LSPR). Real-time SS-OCT monitoring ensures precise evaluation of the soldering process, advancing LTS applications for diverse tissue.</p><p><strong>Study design/materials and methods: </strong>Four solder compositions (C1-C4) are prepared using combinations of BSA, PEG, silver nanoparticles (AgNP) and titanium dioxide nanoparticles (TiNP). Ex-vivo samples of chicken breast, chicken skin, and goat skin were incised in 1 cm incision with 0.45 mm width and soldered using a 980 nm, 5 W laser. Tensile strength was measured using a tensiometer, while cytotoxicity was assessed using HEK293 cells. SS-OCT captured real-time scattering coefficient changes during soldering, providing insight into coagulation dynamics.</p><p><strong>Results: </strong>Combining bovine serum albumin (BSA) with PEG and nanoparticles (silver and titanium dioxide), tensile strength in ex-vivo tissue samples increased significantly-by 27% in chicken breast (0.4980 to 0.6366 N/cm²), 28% in chicken skin (0.6080 to 0.7840 N/cm²), and 23% in goat skin (0.6220 to 0.7666 N/cm²). Nanoparticle incorporation reduced soldering time by 33%, achieving complete fusion within 3 min using a laser of optical power of 5 W, central wavelength 980 nm and duty cycle of 50%. Real-time monitoring with Swept-Source Optical Coherence Tomography (SS-OCT) quantified the scattering coefficient changes during soldering, validating efficient bonding. Results demonstrate PEG's contribution to tensile strength, nanoparticles' role in reducing soldering time, and SS-OCT's utility for precision monitoring, supporting LTS as a promising wound closure method.</p><p><strong>Conclusion: </strong>The study validates PEG's biomechanical reinforcement and nanoparticles' role in efficient LTS. The integration of SS-OCT enables precise, real-time assessment, confirming the clinical potential of this enhanced LTS method for rapid and robust tissue closure.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety Assessment of a 1940 nm Tm:YAP Laser for Fractional Skin Ablation in a Swine Model.","authors":"Yuval Ramot, Michal Steiner, Udi Vazana, Rotem Nahear, Neria Suliman, David J Friedman, Salman Noach, Abraham Nyska","doi":"10.1002/lsm.70022","DOIUrl":"https://doi.org/10.1002/lsm.70022","url":null,"abstract":"<p><strong>Objectives: </strong>This exploratory study was aimed to evaluate the safety and preliminary efficacy of Epicare, a 1940 nm thulium-doped yttrium aluminum perovskite (Tm:YAP) laser, for fractional skin ablation in a swine model. The goal was to assess collagen remodeling and tissue responses across varied laser settings to optimize skin resurfacing applications.</p><p><strong>Materials and methods: </strong>Two female domestic swine were subjected to controlled fractional laser ablation using Epicare across 52 marked abdominal sites with varying energy settings. Macroscopic examinations of ablated sites were conducted immediately following ablation and at 1, 3, 7, 14, 22, and 29 days postablation. Histopathological evaluation was conducted immediately posttreatment, and at 1, 7, and 29 days postablation. Observed parameters included epidermal regeneration, dermal remodeling, inflammation, and collagen deposition.</p><p><strong>Results: </strong>Macroscopic evaluations revealed a fractional, clear, and immediate impact of ablation, consisting primarily of erythema and edema, which resolved without complications by Day 14. Histopathological analysis indicated focal, cylinder-like structures associated with necrotic epidermis and dermis, which healed progressively (i.e. from day 1), transitioning to complete epidermal regeneration by Day 7 for most energy settings. By Day 29, advanced collagen deposition and no residual inflammation indicated effective dermal remodeling, consistent with rapid healing and minimal adverse reactions.</p><p><strong>Conclusions: </strong>Epicare demonstrated a favorable safety profile and effective tissue ablation. These findings support the laser's potential for dermatologic applications while emphasizing the need for further investigation to confirm its efficacy and optimal settings in human clinical trials.</p>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}