Lab Animal最新文献_第7页

Production of a heterozygous exon skipping model of common marmosets using gene-editing technology 利用基因编辑技术制作普通狨猴杂合外显子缺失模型

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-30 DOI: 10.1038/s41684-024-01424-0

Kenya Sato, Hiroki Sasaguri, Wakako Kumita, Tetsushi Sakuma, Tomoe Morioka, Kenichi Nagata, Takashi Inoue, Yoko Kurotaki, Naomi Mihira, Michihira Tagami, Ri-ichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Takashi Yamamoto, Makoto Suematsu, Takaomi C. Saido, Erika Sasaki

{"title":"Production of a heterozygous exon skipping model of common marmosets using gene-editing technology","authors":"Kenya Sato, Hiroki Sasaguri, Wakako Kumita, Tetsushi Sakuma, Tomoe Morioka, Kenichi Nagata, Takashi Inoue, Yoko Kurotaki, Naomi Mihira, Michihira Tagami, Ri-ichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Takashi Yamamoto, Makoto Suematsu, Takaomi C. Saido, Erika Sasaki","doi":"10.1038/s41684-024-01424-0","DOIUrl":"10.1038/s41684-024-01424-0","url":null,"abstract":"Nonhuman primates (NHPs), which are closely related to humans, are useful in biomedical research, and an increasing number of NHP disease models have been reported using gene editing. However, many disease-related genes cause perinatal death when manipulated homozygously by gene editing. In addition, NHP resources, which are limited, should be efficiently used. Here, to address these issues, we developed a method of introducing heterozygous genetic modifications into common marmosets by combining Platinum transcription activator-like effector nuclease (TALEN) and a gene-editing strategy in oocytes. We succeeded in introducing the heterozygous exon 9 deletion mutation in the presenilin 1 gene, which causes familial Alzheimer’s disease in humans, using this technology. As a result, we obtained animals with the expected genotypes and confirmed several Alzheimer’s disease-related biochemical changes. This study suggests that highly efficient heterozygosity-oriented gene editing is possible using TALEN and oocytes and is an effective method for producing genetically modified animals. This study shows that using gene-editing technology in oocytes using Platinum TALEN is an effective method for producing genetically modified marmosets by exon skipping.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"244-251"},"PeriodicalIF":5.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01424-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142100827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial metabolite affects CKD progression 微生物代谢物影响慢性肾脏病的进展

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-30 DOI: 10.1038/s41684-024-01429-9

Alexandra Le Bras

引用次数: 0

Resveratrol improves rat infertility 白藜芦醇可改善大鼠不育症

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-30 DOI: 10.1038/s41684-024-01434-y

Jorge Ferreira

引用次数: 0

Sex differences in microbiota 微生物群的性别差异

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-30 DOI: 10.1038/s41684-024-01430-2

Alexandra Le Bras

引用次数: 0

Challenges and future directions of SUDEP models SUDEP 模型的挑战和未来方向。

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-26 DOI: 10.1038/s41684-024-01426-y

JiaXuan Gu, WeiHui Shao, Lu Liu, YuLing Wang, Yue Yang, ZhuoYue Zhang, YaXuan Wu, Qing Xu, LeYuan Gu, YuanLi Zhang, Yue Shen, HaiTing Zhao, Chang Zeng, HongHai Zhang

{"title":"Challenges and future directions of SUDEP models","authors":"JiaXuan Gu, WeiHui Shao, Lu Liu, YuLing Wang, Yue Yang, ZhuoYue Zhang, YaXuan Wu, Qing Xu, LeYuan Gu, YuanLi Zhang, Yue Shen, HaiTing Zhao, Chang Zeng, HongHai Zhang","doi":"10.1038/s41684-024-01426-y","DOIUrl":"10.1038/s41684-024-01426-y","url":null,"abstract":"Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death among patients with epilepsy, causing a global public health burden. The underlying mechanisms of SUDEP remain elusive, and effective prevention or treatment strategies require further investigation. A major challenge in current SUDEP research is the lack of an ideal model that maximally mimics the human condition. Animal models are important for revealing the potential pathogenesis of SUDEP and preventing its occurrence; however, they have potential limitations due to species differences that prevent them from precisely replicating the intricate physiological and pathological processes of human disease. This Review provides a comprehensive overview of several available SUDEP animal models, highlighting their pros and cons. More importantly, we further propose the establishment of an ideal model based on brain–computer interfaces and artificial intelligence, hoping to offer new insights into potential advancements in SUDEP research. In doing so, we hope to provide valuable information for SUDEP researchers, offer new insights into the pathogenesis of SUDEP and open new avenues for the development of strategies to prevent SUDEP. This Review provides a comprehensive overview of several available animal models of sudden unexpected death in epilepsy (SUDEP), highlighting their pros and cons to elucidate SUDEP causes and mechanisms.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 9","pages":"226-243"},"PeriodicalIF":5.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A closer look at the Laboratory Animal Genetic Reporting (LAG-R) guidelines 实验动物遗传学报告 (LAG-R) 指南详解

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-19 DOI: 10.1038/s41684-024-01427-x

Alexandra Le Bras

引用次数: 0

The highly pathogenic H5N1 virus found in U.S. dairy cattle has some characteristics that could enhance infection and transmission among mammals 在美国奶牛身上发现的高致病性 H5N1 病毒具有一些特征，可增强哺乳动物之间的感染和传播能力

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-14 DOI: 10.1038/s41684-024-01425-z

Fabien Filaire, Sander Herfst

引用次数: 0

Effects of volume management on free flap perfusion and metabolism in a large animal model study 在一项大型动物模型研究中，容量管理对游离皮瓣灌注和新陈代谢的影响

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-09 DOI: 10.1038/s41684-024-01410-6

Daniel G. E. Thiem, Daniel Stephan, Alexander Ziebart, Robert Ruemmler, Julian Riedel, Shankeeth Vinayahalingam, Bilal Al-Nawas, Sebastian Blatt, Peer W. Kämmerer

{"title":"Effects of volume management on free flap perfusion and metabolism in a large animal model study","authors":"Daniel G. E. Thiem, Daniel Stephan, Alexander Ziebart, Robert Ruemmler, Julian Riedel, Shankeeth Vinayahalingam, Bilal Al-Nawas, Sebastian Blatt, Peer W. Kämmerer","doi":"10.1038/s41684-024-01410-6","DOIUrl":"10.1038/s41684-024-01410-6","url":null,"abstract":"Free flap failure represents a substantial clinical burden. The role of intraoperative volume management remains controversial, with valid studies lacking. Here, using a large animal model, we investigated the influence of volume management on free flap perfusion and metabolism. Autotransfer of a musculocutaneous gracilis flap was performed on 31 German domestic pigs, with arterial anastomosis and catheterization of the pedicle vein for sequential blood sampling. Flap reperfusion was followed by induction of a hemorrhagic shock with maintenance for 30 min and subsequent circulation stabilization with crystalloid solution, crystalloid solution and catecholamine, autotransfusion or colloidal solution. Flap perfusion and oxygenation were periodically assessed using hyperspectral imaging. Flap metabolism was assessed via periodic blood gas analyses. Hyperspectral imaging revealed no difference in either superficial or deep tissue oxygen saturation, tissue hemoglobin or tissue water content between the test groups at any time point. Blood gas analyses showed that lactate levels were significantly increased in the group that received crystalloid solution and catecholamine, after circulatory stabilization and up to 2 h after. We conclude that, in hemorrhagic shock, volume management impacts acid–base balance in free flaps. Crystalloid solutions with norepinephrine increase lactate levels, yet short-term effects on flap perfusion seem minimal, suggesting that vasopressors are not detrimental. Free flap is widely used in head and neck reconstructive surgery, but fluid management of surgical patients remains controversial. Here, Thiem et al. used a pig model to monitor the effects of volume management on free flap perfusion and metabolism.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 10","pages":"268-275"},"PeriodicalIF":5.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01410-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatic stress in acute kidney injury 急性肾损伤的肝脏压力

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-01 DOI: 10.1038/s41684-024-01415-1

Jorge Ferreira

引用次数: 0

Humidity and heat cause anxiety in mice 潮湿和高温会导致小鼠焦虑。

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-08-01 DOI: 10.1038/s41684-024-01418-y

Alexandra Le Bras

引用次数: 0