Lab Animal最新文献_第6页

Comparing microglia models 比较小胶质细胞模型

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-26 DOI: 10.1038/s41684-024-01483-3

Alexandra Le Bras

引用次数: 0

COVID-19 affects vitamin D metabolism in the kidney COVID-19 影响肾脏中的维生素 D 代谢

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-26 DOI: 10.1038/s41684-024-01481-5

Jorge Ferreira

引用次数: 0

Stress susceptibility markers 压力易感性标记

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-26 DOI: 10.1038/s41684-024-01489-x

Jorge Ferreira

引用次数: 0

Sex differences in response to stress and alcohol abuse 对压力和酗酒反应的性别差异

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-26 DOI: 10.1038/s41684-024-01486-0

Jorge Ferreira

引用次数: 0

Revisited guidelines for metabolic tolerance tests in mice 重新修订小鼠代谢耐受性试验指南

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-25 DOI: 10.1038/s41684-024-01473-5

Cedric Moro, Christophe Magnan

{"title":"Revisited guidelines for metabolic tolerance tests in mice","authors":"Cedric Moro, Christophe Magnan","doi":"10.1038/s41684-024-01473-5","DOIUrl":"10.1038/s41684-024-01473-5","url":null,"abstract":"Preclinical mouse models are extensively used in biomedical research to gain insight into disease mechanisms and to test new drug treatments. Glucose and insulin tolerance tests are simple experimental tests frequently used worldwide to assess glucose metabolism in mice. Various guidelines and methodological considerations have been published to help researchers standardize procedures and optimize research outcomes. Yet, there is still important experimental heterogeneity in the way these simple procedures are performed, with no real consensus on what the best practices are to achieve high-quality research and reproducible results. Here we critically examine several published guidelines and recent technical reports on how to perform these metabolic tests in laboratory mice and discuss the influence of various confounding factors on test results. We hope this work will help scientists establish more consensual guidelines for maximizing the relevance and clinical translation of studies using mouse models in metabolic research. In this Review, the authors examine current guidelines for metabolic tolerance tests in mice and provide a set of revisited recommendations to improve the reproducibility and clinical translation of the findings.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"54 1","pages":"16-23"},"PeriodicalIF":5.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01473-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A revision to marble burying: video analysis during the marble burying task is imperative to understanding rodent behavior 对埋弹珠任务的修订：要了解啮齿动物的行为，必须对埋弹珠任务进行视频分析

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-22 DOI: 10.1038/s41684-024-01459-3

Lauren A. Burgett, Theres B. Alexander, Haley A. Moya, Marcy A. Kingsbury

{"title":"A revision to marble burying: video analysis during the marble burying task is imperative to understanding rodent behavior","authors":"Lauren A. Burgett, Theres B. Alexander, Haley A. Moya, Marcy A. Kingsbury","doi":"10.1038/s41684-024-01459-3","DOIUrl":"10.1038/s41684-024-01459-3","url":null,"abstract":"The translational value of the marble burying task (MBT) is debatable. Here we performed video analysis of behaviors during the MBT to accurately capture the details of all behaviors displayed during this task. Our results show that a count of marbles buried at the end of the task may not be a good translational correlate of the ‘intentional marble burying’ that the task is assumed to measure. Rather, the number of marbles buried may be measuring accidental marble burying due to ‘rapid digging’. Video analysis during MBT provides a novel approach to characterize the many behaviors displayed during this task and may explain inconsistencies reported in the literature. However, the number of marbles buried at the end of the MBT may still have value as a screening test for anxiogenic or anxiolytic interventions. Any interventions that show significant alterations in the number of marbles buried can be pursued further through more robust and comprehensive behavior scoring methods. The marble burying task (MBT) is used to measure anxiety-like behavior, relying on a snapshot of the number of marbles buried at the end of the test. This Brief Communication introduces a more extensive behavioral analysis to improve the translational value of MBT for clinical psychological phenotypes.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 12","pages":"387-389"},"PeriodicalIF":5.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo optical tissue clearing using light-absorbing dyes 使用光吸收染料进行体内光学组织清除

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-15 DOI: 10.1038/s41684-024-01472-6

Maria Victoria Gómez-Gaviro, Vicente Llorente

引用次数: 0

Toward a common interpretation of the 3Rs principles in animal research 实现对动物研究中 3R 原则的共同解释

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-15 DOI: 10.1038/s41684-024-01476-2

Jan Lauwereyns, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, Fernando Gonzalez-Uarquin, Yesim Isil Ulman, Orsolya Ivett Hoffmann, Maria Kitsara, Nikolaos Kostomitsopoulos, Winfried Neuhaus, Benoit Petit-Demouliere, Simone Pollo, Brígida Riso, Sophie Schober, Athanassia Sotiropoulos, Aurélie Thomas, Augusto Vitale, Doris Wilflingseder, Arti Ahluwalia

{"title":"Toward a common interpretation of the 3Rs principles in animal research","authors":"Jan Lauwereyns, Jeffrey Bajramovic, Bettina Bert, Samuel Camenzind, Joery De Kock, Alisa Elezović, Sevilay Erden, Fernando Gonzalez-Uarquin, Yesim Isil Ulman, Orsolya Ivett Hoffmann, Maria Kitsara, Nikolaos Kostomitsopoulos, Winfried Neuhaus, Benoit Petit-Demouliere, Simone Pollo, Brígida Riso, Sophie Schober, Athanassia Sotiropoulos, Aurélie Thomas, Augusto Vitale, Doris Wilflingseder, Arti Ahluwalia","doi":"10.1038/s41684-024-01476-2","DOIUrl":"10.1038/s41684-024-01476-2","url":null,"abstract":"Many scientific breakthroughs have depended on animal research, yet the ethical concerns surrounding the use of animals in experimentation have long prompted discussions about humane treatment and responsible scientific practice. First articulated by Russell and Burch, the 3Rs Principles of Replacement, Reduction, and Refinement have gained widespread recognition as basic guidelines for animal research. Over time, the 3Rs have transcended the research community, influencing policy decisions, animal welfare advocacy and public perception of animal experimentation. Despite their broad acceptance, interpretations of the 3Rs vary substantially, shaping statutory frameworks at various levels, with both technical and practical impacts.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 12","pages":"347-350"},"PeriodicalIF":5.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01476-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Murine model of minimally invasive nasal depot (MIND) technique for central nervous system delivery of blood–brain barrier-impermeant therapeutics 用于中枢神经系统输送血脑屏障渗透疗法药物的微创鼻腔药库（MIND）小鼠模型

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-15 DOI: 10.1038/s41684-024-01460-w

Andy J. Chua, Valentina Di Francesco, Anisha D’Souza, Mansoor Amiji, Benjamin S. Bleier

{"title":"Murine model of minimally invasive nasal depot (MIND) technique for central nervous system delivery of blood–brain barrier-impermeant therapeutics","authors":"Andy J. Chua, Valentina Di Francesco, Anisha D’Souza, Mansoor Amiji, Benjamin S. Bleier","doi":"10.1038/s41684-024-01460-w","DOIUrl":"10.1038/s41684-024-01460-w","url":null,"abstract":"The blood–brain barrier (BBB) poses a substantial obstacle to the successful delivery of therapeutics to the central nervous system (CNS). The transnasal route has been extensively explored, but success rates have been modest due to challenges related to the precise anatomical placement of drugs, the small volumes that the olfactory cleft can accommodate and short drug residence times due to mucociliary clearance. Here, to address these issues, we have developed a surgical technique known as the minimally invasive nasal depot (MIND), which allows the accurate placement of depot drugs into the submucosal space of the olfactory epithelium of rats. This technique exploits the unique anatomy of the olfactory apparatus to enable transnasal delivery of drugs into the CNS, bypassing the BBB. In our rat model, a bony window is created in the animal snout to expose the submucosal space. Using the MIND technique, we have successfully delivered oligonucleotides to the CNS in Sprague-Dawley and Long-Evans rats, leading to an upregulation of brain-derived neurotrophic factor in the substantia nigra and hippocampus. In this Protocol, we describe the procedural steps for MIND. This procedure takes about 45 min and can be performed by researchers with basic surgical skills. We additionally describe modifications to perform MIND in mice, which are anatomically smaller. The MIND procedure represents a unique platform that can be used to overcome the limitations posed by the BBB. This technique can potentially expand the therapeutic toolkit in the treatment of neurological diseases. This Protocol describes a minimally invasive technique for central nervous system drug delivery in rodents. The procedure allows the use of the transnasal route to deliver blood–brain barrier-impermeant drugs.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 12","pages":"363-375"},"PeriodicalIF":5.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and evaluation of an autism pig model 自闭症猪模型的开发与评估

IF 5.9 3区农林科学

Lab Animal Pub Date : 2024-11-12 DOI: 10.1038/s41684-024-01475-3

Shuai Qiu, Jingyan Jia, Benlei Xu, Nan Wu, Huaqiang Cao, Shuangyi Xie, Jialong Cui, Ji Ma, Yi-Hsuan Pan, Xiao-Bing Yuan

{"title":"Development and evaluation of an autism pig model","authors":"Shuai Qiu, Jingyan Jia, Benlei Xu, Nan Wu, Huaqiang Cao, Shuangyi Xie, Jialong Cui, Ji Ma, Yi-Hsuan Pan, Xiao-Bing Yuan","doi":"10.1038/s41684-024-01475-3","DOIUrl":"10.1038/s41684-024-01475-3","url":null,"abstract":"Developing cost-effective and disease-relevant animal models is essential for advancing biomedical research into human disorders. Here we investigate the feasibility of a pig model for autism spectrum disorder (ASD) using embryonic exposure to valproic acid (VPA), an antiepileptic drug known to increase ASD risk. We established experimental paradigms to assess the behavioral characteristics of these pig models. Administration of VPA to Bama miniature pigs (Sus scrofa domestica) during critical embryonic stages resulted in abnormal gait, increased anxiety levels, reduced learning capabilities and altered social patterns, while largely preserving social preference of treated piglets. Notably, we detected significant neuroanatomical changes in cortical regions associated with ASD in the VPA-treated pigs, including cortical malformation, increased neuronal soma size, decreased dendritic complexity and reduced dendritic spine maturation. Transcriptome analysis of the prefrontal cortex of VPA-treated pigs further revealed substantial alterations in the expression of genes linked to ASD, especially genes of the dopamine signaling pathway, highlighting the model’s relevance and potential for shedding light on ASD’s underlying neuropathological and molecular mechanisms. These findings suggest that pig models could serve as a promising alternative to traditional rodent models and provide a more ethical substitute for the use of primates in translational research on neurodevelopmental disorders. This study describes a new pig model of autism induced by embryonic exposure to valproic acid. The pig model displays behavioral alterations, abnormal gait and increased anxiety, which recapitulate key features of autism.","PeriodicalId":17936,"journal":{"name":"Lab Animal","volume":"53 12","pages":"376-386"},"PeriodicalIF":5.9,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41684-024-01475-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0