Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo
{"title":"Protective effect of Allium ochotense Prokh.\u0000 extract against ethanol-induced cytotoxicity","authors":"Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo","doi":"10.11002/kjfp.2023.30.3.526","DOIUrl":"https://doi.org/10.11002/kjfp.2023.30.3.526","url":null,"abstract":"\u0000 \u0000 This study aimed to evaluate the antioxidant and hepatoprotective effects of\u0000 aqueous and 60% ethanol extracts of Allium ochotense\u0000 Prokh. against alcohol-induced cytotoxicity as well as on the activities of\u0000 alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed\u0000 using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl,\u0000 ferric reducing antioxidant power, and malondialdehyde assays, and found that\u0000 both extracts exhibited considerable antioxidant activities. Additionally, both\u0000 extracts showed synergistic effects on the activities of alcohol-metabolic\u0000 enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde\u0000 dehydrogenase. In addition, 2’-7’-dichlorodihydrofluorescein\u0000 diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium\u0000 bromide (MTT) assays revealed that aqueous and 60% ethanol extracts\u0000 reduced oxidative stress and increased cell viability. Moreover, both extracts\u0000 regulated the expression of apoptosis-related proteins, namely B-cell lymphoma\u0000 (BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In\u0000 conclusion, aqueous and 60% ethanol extracts of A.\u0000 ochotense Prokh. might be valuable functional materials derived\u0000 from natural resources for the prevention of ethanol-induced cytotoxicity.\u0000","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49614880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}