Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo
{"title":"葱的保护作用。提取物对抗乙醇诱导的细胞毒性","authors":"Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo","doi":"10.11002/kjfp.2023.30.3.526","DOIUrl":null,"url":null,"abstract":"\n \n This study aimed to evaluate the antioxidant and hepatoprotective effects of\n aqueous and 60% ethanol extracts of Allium ochotense\n Prokh. against alcohol-induced cytotoxicity as well as on the activities of\n alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed\n using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl,\n ferric reducing antioxidant power, and malondialdehyde assays, and found that\n both extracts exhibited considerable antioxidant activities. Additionally, both\n extracts showed synergistic effects on the activities of alcohol-metabolic\n enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde\n dehydrogenase. In addition, 2’-7’-dichlorodihydrofluorescein\n diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium\n bromide (MTT) assays revealed that aqueous and 60% ethanol extracts\n reduced oxidative stress and increased cell viability. Moreover, both extracts\n regulated the expression of apoptosis-related proteins, namely B-cell lymphoma\n (BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In\n conclusion, aqueous and 60% ethanol extracts of A.\n ochotense Prokh. might be valuable functional materials derived\n from natural resources for the prevention of ethanol-induced cytotoxicity.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective effect of Allium ochotense Prokh.\\n extract against ethanol-induced cytotoxicity\",\"authors\":\"Tae Yoon Kim, Jong Min Kim, H. Lee, M. Go, S. Joo, Ju Hui Kim, H. Lee, S. Sim, H. Heo\",\"doi\":\"10.11002/kjfp.2023.30.3.526\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n This study aimed to evaluate the antioxidant and hepatoprotective effects of\\n aqueous and 60% ethanol extracts of Allium ochotense\\n Prokh. against alcohol-induced cytotoxicity as well as on the activities of\\n alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed\\n using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl,\\n ferric reducing antioxidant power, and malondialdehyde assays, and found that\\n both extracts exhibited considerable antioxidant activities. Additionally, both\\n extracts showed synergistic effects on the activities of alcohol-metabolic\\n enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde\\n dehydrogenase. In addition, 2’-7’-dichlorodihydrofluorescein\\n diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium\\n bromide (MTT) assays revealed that aqueous and 60% ethanol extracts\\n reduced oxidative stress and increased cell viability. Moreover, both extracts\\n regulated the expression of apoptosis-related proteins, namely B-cell lymphoma\\n (BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In\\n conclusion, aqueous and 60% ethanol extracts of A.\\n ochotense Prokh. might be valuable functional materials derived\\n from natural resources for the prevention of ethanol-induced cytotoxicity.\\n\",\"PeriodicalId\":17875,\"journal\":{\"name\":\"Korean Journal of Food Preservation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Food Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11002/kjfp.2023.30.3.526\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2023.30.3.526","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Protective effect of Allium ochotense Prokh.
extract against ethanol-induced cytotoxicity
This study aimed to evaluate the antioxidant and hepatoprotective effects of
aqueous and 60% ethanol extracts of Allium ochotense
Prokh. against alcohol-induced cytotoxicity as well as on the activities of
alcohol-metabolic enzymes. Antioxidant effects of the extracts were analyzed
using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl,
ferric reducing antioxidant power, and malondialdehyde assays, and found that
both extracts exhibited considerable antioxidant activities. Additionally, both
extracts showed synergistic effects on the activities of alcohol-metabolic
enzymes, such as alcohol dehydrogenase, but not on the activity of aldehyde
dehydrogenase. In addition, 2’-7’-dichlorodihydrofluorescein
diacetate (DCF-DA) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assays revealed that aqueous and 60% ethanol extracts
reduced oxidative stress and increased cell viability. Moreover, both extracts
regulated the expression of apoptosis-related proteins, namely B-cell lymphoma
(BCl-2), BCl-2 associated X (BAX), and pro-caspase-3, in HepG2 cells. In
conclusion, aqueous and 60% ethanol extracts of A.
ochotense Prokh. might be valuable functional materials derived
from natural resources for the prevention of ethanol-induced cytotoxicity.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.