Journal of the Science of Food and Agriculture最新文献

{"title":"Lacticaseibacillus rhamnosus GG improves behavior, immune functions, and biological age markers in female mice with premature aging.","authors":"Estefanía Díaz-Del Cerro, Judith Félix, Beatriz Solo de Zaldívar, M Carmen Martínez-Cuesta, Noemí Ceprián, Teresa Requena, Mónica De la Fuente","doi":"10.1002/jsfa.14219","DOIUrl":"https://doi.org/10.1002/jsfa.14219","url":null,"abstract":"<p><strong>Background: </strong>Prematurely aged mice (PAM), characterized by an inadequate stress response, exhibit early immunosenescence and reduced lifespan compared with exceptional non-PAM (E-NPAM) of the same age. Lacticaseibacillus rhamnosus GG (LGG) has been proposed as a beneficial probiotic in healthy aging. This study aimed to evaluate the effects of LGG consumption over 2 and 4 weeks on behavioral parameters, peritoneal leukocyte function, and lifespan in adult female PAM.</p><p><strong>Results: </strong>Female ICR-CD1 mice were classified as either PAM or E-NPAM and divided into three groups: (1) PAM control (PAMC), (2) PAM receiving LGG (PAMLGG), and (3) E-NPAM control (E-NPAMC). The PAMLGG group consumed a lyophilized LGG suspension in skim milk for 4 weeks, while the PAMC and E-NPAMC groups received a skim milk solution. Behavioral tests were conducted after 2 weeks, and leukocyte function was analyzed after 2 and 4 weeks. The results showed that the PAMLGG group exhibited improved behavioral responses and enhanced immune functions, which was reflected in a lower biological age and in a longer longevity compared with the PAMC.</p><p><strong>Conclusion: </strong>LGG supplementation positively influenced behavior, immune function, and biological age markers in female PAM, leading to increased longevity. These findings indicate that LGG administration could be a good nutritional strategy for slowing down the aging process, allowing a healthy longevity to be achieved. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic immunomodulatory effect of wheat/soybean/sea cucumber peptides in chloramphenicol induced immunosuppression zebrafish","authors":"Qianqian Wang,&nbsp;Yiyang Xie,&nbsp;Junhui Zhang,&nbsp;Minjie Zhao,&nbsp;Jing Wang,&nbsp;Juan Du,&nbsp;Weihong Min,&nbsp;Fengqin Feng,&nbsp;Fei Shen","doi":"10.1002/jsfa.14130","DOIUrl":"10.1002/jsfa.14130","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> BACKGROUND</h3>\u0000 \u0000 <p>Food-derived immunomodulatory peptides serve as vital agents in promoting health by protecting the body against pathogens. The present study focused on determining the optimal ratio for combining different peptides to form a complex that enhances immune activity in immunosuppressed zebrafish.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> RESULTS</h3>\u0000 \u0000 <p>We established a method for immunosuppression in zebrafish, discovering that treatment with 125 μg mL⁻¹ chloramphenicol significantly decreased the macrophage number and neutrophil fluorescence intensity. We then assessed the immunomodulatory activity of soft-shelled turtle peptides, sea cucumber peptides (SCP), black-bone silky fowl peptides, soybean peptides (SP), wheat peptides (WP), whey protein peptides and casein hydrolysate peptides. The results of individual peptides showed that WP uniquely increased the interferon-γ level, SP most effectively increased neutrophil fluorescence intensity, and SCP increased both macrophage number and neutrophil fluorescence intensity. Using mixture design and fuzzy comprehensive evaluation methods, we identified a synergistic mixture peptides: 8.32% SCP + 20.84% SP + 70.84% WP, which had the highest score in the fuzzy comprehensive evaluation, making it the best blend for boosting zebrafish immunity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> CONCLUSION</h3>\u0000 \u0000 <p>A specific mixture of peptides (8.32% SCP + 20.84% SP + 70.84% WP) holds potential as a result of a synergistic immunomodulatory effect. © 2025 Society of Chemical Industry.</p>\u0000 </section>\u0000 </div>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":"105 7","pages":"3712-3721"},"PeriodicalIF":3.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin delivery based on bovine bone protein hydrolysate-flaxseed gum emulsion carrier: a strategy for stability enhancement and bioaccessibility improvement.","authors":"Niancheng Hong, Shengnan Liu, Yaodi Zhu, Miaoyun Li, Lijun Zhao, Dong Liang, Gaiming Zhao","doi":"10.1002/jsfa.14209","DOIUrl":"https://doi.org/10.1002/jsfa.14209","url":null,"abstract":"<p><strong>Background: </strong>Curcumin is a natural polyphenolic compound that exhibits various biological activities. However, its low solubility in water and instability significantly limit its potential application in the food industry. Therefore, appropriate encapsulation systems are required to address these limitations.</p><p><strong>Results: </strong>This study initially examined the structural changes of bovine bone protein hydrolysate (BBPH) after its combination with flaxseed gum (FG) and subsequently evaluated the encapsulation properties of BBPH-FG emulsions for curcumin. The results showed that, in an aqueous solution, BBPH and FG formed a complex through hydrophobic and electrostatic interactions, which significantly enhanced the surface hydrophobicity of the BBPH and improved its thermal stability (145.1 °C). Encapsulation of curcumin in the emulsions revealed that its incorporation had a minimal effect on the overall stability of the BBPH-FG emulsion system. The encapsulation efficiency reached 77.51% when the curcumin concentration was 0.2%. At this concentration, the curcumin emulsions demonstrated scavenging efficiencies of 62.69%, 86.98% and 79.98% for OH radicals, DPPH radicals and ABTS radicals, respectively. Furthermore, the results from in vitro simulated digestion experiments indicated that the digestive stability and bioaccessibility of curcumin following emulsion encapsulation were 77.47% and 67.36%, respectively, both of which were markedly higher than those of curcumin dissolved in the oil phase.</p><p><strong>Conclusion: </strong>This study demonstrates that encapsulation via a BBPH-FG emulsion system can significantly enhance the stability and bioaccessibility of curcumin during in vitro simulated digestion. These findings provide valuable insights into the construction of food-grade emulsion delivery systems and offer potential avenues for expanding the applications of curcumin. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability and molecular interactions in cold-induced hazelnut protein-chlorogenic acid gel.","authors":"Xuemei Wang, Jiarong Wang, Ligang Zhang, Wenqi Wang, Lai Jiang, Yuhua Yang, Yuhong Zhao","doi":"10.1002/jsfa.14192","DOIUrl":"https://doi.org/10.1002/jsfa.14192","url":null,"abstract":"<p><strong>Background: </strong>Phenols have been demonstrated to enhance protein gelation but their stability is often compromised in heat-induced gels. This has prompted the development of cold-induced gels. The objective of this study was to enhance the gel properties of hazelnut isolate protein (HPI) through the incorporation of transglutaminase (TGase), glucono-δ-lactone (GDL), and chlorogenic acid (CA). It also aimed to investigate the biofunctional role of CA.</p><p><strong>Results: </strong>In comparison with heat-induced gels, cold-induced gels exhibited a higher prevalence of disulfide (25.51%) and hydrogen bonds (35.88%). Transglutaminyl transferase also facilitated the formation of ε-(γ-glutamyl)lysine interpeptide bonds, enhancing the gel properties of cold-induced gels significantly. The gel strength increased by about 3.9 times, the water-holding capacity increased by approximately 2.8 times, and storage modulus increased by about 2.5 times. The resulting gels were more compact and stable, which reduced protein digestibility. It was observed that the interaction between HPI and CA in cold-induced gels was significantly enhanced, thereby enabling CA to improve the gelation capability of HPI more effectively, particularly in TGase-induced formulations. Chlorogenic acid displayed strong thermal stability in cold-induced gels; findings from in vitro digestion simulation also indicated that the CA present in HPI exhibited effective sustained release properties within the gastrointestinal tract.</p><p><strong>Conclusion: </strong>The present study offers novel approaches to the utilization of HPI in food applications and presents innovative strategies for developing HPI gel foods characterized by enhanced gel properties and rich active compound content. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palm-based nanocrystal cellulose-stabilized Pickering emulsions: investigating characteristics, stability and in vitro digestion for potential application as substitution of coconut milk.","authors":"Wai-Ting Chong, Lee Fong Siow, Eng-Seng Chan, Beng Ti Tey, Yee-Ying Lee","doi":"10.1002/jsfa.14200","DOIUrl":"https://doi.org/10.1002/jsfa.14200","url":null,"abstract":"<p><strong>Background: </strong>Coconut milk encounters challenges related to global sustainability and its high fat content, which may potentially have adverse effects on health. Nanocrystal cellulose (NCC) has gained attention due to its amphiphilic nature, high aspect ratio and large elastic modulus, making it a beneficial natural stabilizer for emulsion stabilization. Recent studies have demonstrated that NCC plays a role in modulating gastrointestinal digestion. Therefore, this study aims to produce a Pickering emulsion stabilized by NCC (NCC-PE) as a potential substitute for coconut milk. The study evaluated the capability of NCC in stabilizing a Pickering emulsion. NCC-PE was exposed to simulated gastrointestinal digestion and the results were compared with those for lecithin-stabilized Pickering emulsion and commercial coconut milk.</p><p><strong>Results: </strong>In terms of d₃₂, viscosity and creaming index, 4 g kg^-1 of NCC effectively stabilized 200 g kg^-1 (20%) of palm oil to formulate Pickering emulsions with a mean d₃₂ of 4 μm and zeta potential of -49.09 ± 1.63 mV and demonstrating high stability against creaming. NCC-PE remains stable for at least 14 days when stored at room temperature (25 °C). The d₃₂ and zeta potential of Pickering emulsions were evaluated under different pH (4-10), temperature (-18 to 75 °C) and ionic strength (0-250 mmol L^-1), exhibiting satisfactory performance and high stability against creaming, except at pH 2 and 100 °C. Results indicated slower lipid hydrolysis in NCC-PE (62.47 ± 2.5%) compared with coconut milk (67.9 ± 1.14%) in a simulated gastrointestinal model.</p><p><strong>Conclusion: </strong>Our results highlight the potential of NCC-PE to act as substitute for coconut milk, influencing the release of free fatty acids. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Z-Astaxanthin exhibits superior anti-obesity effects in Caenorhabditis elegans: insights from geometric isomers and signaling pathways.","authors":"Junting Xie, Rongqian Cai, Xiaoning Hou, Kaixin Zhao, Jie Xiao, Yong Cao, Xiaojuan Liu","doi":"10.1002/jsfa.14202","DOIUrl":"https://doi.org/10.1002/jsfa.14202","url":null,"abstract":"<p><strong>Background: </strong>Astaxanthin (AST) exists as an 'all-E' isomer and various 'Z' isomers due to its multiple conjugated double bonds. The Z configuration has been reported to exhibit stronger antioxidant activity, raising interest in its potential health benefits. All-E AST is a potent chemopreventive agent for weight loss but the anti-obesity efficacy of Z isomers remains unclear. This study therefore explored the role of geometric isomers (all-E, 9-Z, and 13-Z) of AST on the alleviation of obesity using Caenorhabditis elegans, a well established model organism.</p><p><strong>Results: </strong>All-E, 9-Z, and 13-Z AST reduced obesity of high-fat worms substantially. Triglyceride (TG) decreased by 18.84%, with all-E AST, 41.18%, with 9-Z AST, and 35.94% with 13-Z AST (P < 0.05), indicating that Z AST displayed a superior anti-obesity effect. Interestingly, AST, and particularly its Z-isomers, reduced large lipid droplets (LDs) and oleic acid/stearic acid (C18:1Δ9/C18:0) significantly; these contribute to lipid accumulation. Astaxanthin also minimized food intake and boosted energy consumption, and Z-isomers outperformed all-E in enhancing mobility. Using quantitative polymerase chain reaction (qPCR), green fluorescent protein binding, and gene-deficient nematodes, the findings of the current study indicated that AST, especially Z isomers, suppressed key gene expression in sbp-1/mdt-15 and insulin/insulin-like growth factor signaling (IIS) pathways, which are responsible for regulating fatty acid composition and energy balance, and subsequently co-downregulated the essential genes fat-6 and fat-7 involved in C18:1Δ9 synthesis.</p><p><strong>Conclusion: </strong>This study provides important insights into the association between anti-obesity effects and geometric isomers of carotenoids, guiding their application in the health field. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of the transcriptomics and metabolomics reveal the changes induced by nano-selenium and melatonin in Zizyphus jujuba Mill. cv. Huizao.","authors":"Yuting Yuan, Defen Liu, Jiefei Mao, Hejiang Liu, Rongrong Wang, Yanjun Ju, Wanjin Sun, Wenzhao Jia, Yi Fang, Canping Pan, Shengjun Ma, Lu Kang","doi":"10.1002/jsfa.14203","DOIUrl":"https://doi.org/10.1002/jsfa.14203","url":null,"abstract":"<p><strong>Background: </strong>Jujube (Zizyphus jujuba), belonging to the Rhamnaceae, contains various bioactive components including flavonoids and polysaccharides. In our previous study, jujube quality was improved by application of nano-selenium (Nano-Se) and melatonin; however, little research has been conducted on the induced molecular mechanisms. Zizyphus jujuba cv. Huizao was used as experimental materials to compare and analyze the transcriptomics and metabolomics mechanisms.</p><p><strong>Results: </strong>Compared with the control, 305, 422, and 240 metabolites and 24, 281, and 44 genes were significantly changed at the metabolic and transcriptional levels in Nano-Se, melatonin, and Nano-Se with melatonin treatments, respectively. The metabolomics indicated that the differentially expressed metabolites were involved in phenylpropanoid biosynthesis, fructose, and mannose metabolic pathways. The Nano-Se with melatonin treatment group increased the contents of glucose and total Se by 1.8 and 5.4 times, respectively. Transcriptomics showed that differentially expressed genes of Nano-Se and melatonin treatment were mainly enriched in phenylpropanoid biosynthesis, pentose, and glucuronate interconversions pathways. In addition, several transcription factors from the NAC, WRKY, MYB, and WRKY families were identified in melatonin versus control and melatonin versus Nano-Se. Furthermore, combined transcriptomic and metabolomic analysis showed that phenylpropanoid biosynthesis, starch and sucrose metabolism, the mutual conversion pathway of pentose, and glucuronate interconversions were the vital pathways in response to Nano-Se and melatonin treatment.</p><p><strong>Conclusion: </strong>Overall, these results provide new insights into jujube response to Nano-Se and melatonin, and improve our understanding of the jujube molecular mechanisms induced by Nano-Se and melatonin. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo effects of Centella asiatica on intestinal inflammatory models and identification of its bioactive components.","authors":"Hyun Young Shin, Yeon Suk Kim, Mi Yeun Joung, Woo Jung Kim, Kwang-Soon Shin, Kwang-Won Yu","doi":"10.1002/jsfa.14213","DOIUrl":"https://doi.org/10.1002/jsfa.14213","url":null,"abstract":"<p><strong>Background: </strong>The family Apiaceae (genus Centella) includes approximately 50 species, many of which are used in traditional medicine to treat various inflammatory diseases. Traditionally, Centella asiatica has been widely used as a medicinal herb in Ayurvedic medicine. The efficacy of C. asiatica in treating intestinal inflammation remains poorly explored.</p><p><strong>Results: </strong>This study investigated the effects of a hot-water extract of C. asiatica (CA-HE) on inflammation-induced intestinal epithelial cell lines and animal models. CA-HE effectively alleviated IL-1β-induced inflammatory factors and tight junction (TJ) proteins. In a dextran sulfate sodium (DSS)-induced mouse model of colitis, oral administration of CA-HE induced no toxicological effects and significantly alleviated major clinical symptoms of colitis. Examination of colon specimens revealed that oral administration of CA-HE significantly prevented DSS-induced inflammatory and TJ-associated factors in mice. Histopathological examination of hematoxylin and eosin- and periodic acid-Schiff/Alcian blue-stained colon sections showed that CA-HE alleviated crypt destruction, submucosal edema, inflammatory infiltration and mucin secretion compared to the DSS group. Oral administration of CA-HE increased the total levels of short-chain fatty acids in the cecum and feces. Finally, UHPLC-MS analysis predicted that CA-HE comprises five phenolic compounds (neochlorogenic acid, cryptochlorogenic acid, 1,5-dicaffeoylquinic acid (DCQA), 3,5-DCQA and 4,5-DCQA) and one flavonoid (miquelianin) as key bioactive components.</p><p><strong>Conclusion: </strong>Our results suggest that CA-HE could be an alternative candidate to prevent and treat intestinal inflammation. Furthermore, our findings provide foundational data for the use of CA-HE as a bioactive component. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the complex coacervation behavior and interaction mechanism of nisin and rhamnolipid.","authors":"Jing-Kun Yan, Zi-Wei Wang, Lin Wang, Zheng-Cai Liu, Ya-Hui Yu, Mingyu Jin, Long-Qing Li, Lin Li","doi":"10.1002/jsfa.14204","DOIUrl":"https://doi.org/10.1002/jsfa.14204","url":null,"abstract":"<p><strong>Background: </strong>Nisin is a natural, safe and highly effective food preservative that can inhibit the growth and reproduction of most gram-positive bacteria. However, the antimicrobial effect of nisin shows poor stability, which is a disadvantage in practical applications. Microencapsulation of nisin through complex coacervation addresses its poor antibacterial stability and limited spectrum. This study investigated the phase behavior, microstructural properties, and interaction mechanisms between nisin and rhamnolipid (RL).</p><p><strong>Results: </strong>The results showed that the characteristic boundary pH values (pH_c, pH_φ1, pH_opt, pH_φ2) of the nisin/RL complex coacervates increased significantly by decreasing the RL to nisin mass ratio from 1:12 to 1:16 (w/w). At different critical pH values, nisin/RL complexes and coacervates exhibited varied microstructural properties, with those formed at pH_opt (4.4-4.8) displaying the most intense microstructures. Electrostatic attractions were the primary driving force to give nisin/RL complex coacervation, with hydrophobic and hydrogen bond interactions playing fewer roles. Notably, complex coacervation, particularly at pH_opt, enhanced the thermal stability of nisin but reduced its crystallinity and secondary structure.</p><p><strong>Conclusion: </strong>Therefore, our findings offer a theoretical basis for stabilizing the antibacterial agent nisin through complex coacervation. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion-pairing reversed-phase chromatography with inductively coupled plasma-mass spectrometry applied to simultaneous determination of arsenic and selenium species in functional food American ginseng.","authors":"Jianjie Wu, Yuanyuan Zhang, Ping Sheng, Chunjiao He, Hao Liu, Shanyong Gu, Jiaoyang Luo, Meihua Yang","doi":"10.1002/jsfa.14211","DOIUrl":"https://doi.org/10.1002/jsfa.14211","url":null,"abstract":"<p><strong>Background: </strong>The toxicity of heavy metals and harmful elements is highly dependent on their chemical species. However, research on the simultaneous analysis of inorganic and organic species in complex matrices remains limited. This study presents a rapid and sensitive method for the concurrent determination of arsenic and selenium species using reversed-phase chromatography with inductively coupled plasma-mass spectrometry. Enzyme-assisted extraction was employed to enhance the efficiency of target analyte recovery.</p><p><strong>Results: </strong>By utilizing ion-pairing reagents and a mobile phase consisting of 20 mmol L^-1 citric acid with 2.0% methanol at pH 4.0, six arsenic species and five selenium species were simultaneously analyzed in kinetic energy discrimination mode within 10 min. The limits of detection for arsenic and selenium species ranged from 0.058 to 0.6 μg kg^-1 and 0.083 to 0.283 μg kg^-1, respectively. The precision of the spiked American ginseng samples ranged from 1.07% to 11.08%, with recovery rates between 72.13% and 107.2%. The method successfully identified and quantified 11 elemental species.</p><p><strong>Conclusion: </strong>The analysis of real American ginseng samples revealed that the predominant arsenic species were inorganic arsenic(V), while the primary selenium species was organic selenomethionine. This study provides a valuable and efficient method for the simultaneous monitoring of elemental species in functional foods, contributing to their safety assessment and quality control. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}