Journal of the Science of Food and Agriculture最新文献

{"title":"Effects of oat protein on hematological parameters and metabolism in Wistar rats.","authors":"Gabriela Matte Bertoldi, Karine Raquel Uhdich Kleibert, Flávia Alessandra da Silva Räder, Alana Thais Gisch Andres, Luciana Mori Viero, Maria Andréia Inkelmann, José Antonio Gonzalez da Silva, Ivan Ricardo Carvalho, Christiane de Fátima Colet","doi":"10.1002/jsfa.71164","DOIUrl":"https://doi.org/10.1002/jsfa.71164","url":null,"abstract":"<p><strong>Background: </strong>Diets rich in plant-based proteins are becoming increasingly popular, but the safety of extracted oat (Avena sativa L.) protein in relation to cardiometabolic and endocrine risks remains unexplored. This study evaluated the safety of oat protein by analyzing anthropometric, hematological, biochemical, and histopathological parameters in male Wistar rats fed high-protein diets for 30 or 90 days.</p><p><strong>Results: </strong>Animals were divided into four diet groups (n = 6 per group): oat protein-supplemented (G1), soy protein-supplemented (G2), casein-supplemented (G3), and control (G4). After 30 days, the oat protein group had significantly higher leukocyte (P = 0.018) and lymphocyte (P = 0.001) counts than the other groups. After 90 days, this group had significantly lower erythrocyte counts (P = 0.007), hemoglobin levels (P = 0.017), hematocrit values (P = 0.011), total leukocyte counts (P = 0.001), lymphocyte counts (P = 0.001), and platelet counts (P = 0.001) than the other groups. Oat protein supplementation improved lipid parameters, reducing low-density lipoprotein (LDL) cholesterol (P = 0.034) after 30 days and triglycerides (P = 0.003) and very-low-density lipoprotein (VLDL) cholesterol (P = 0.003) after 90 days, with effects similar to those observed in the casein group. Increased creatinine levels (P = 0.005) were observed in the oat protein group after 30 days, suggesting a transient renal effect. Histopathological analysis revealed mild to moderate alterations in liver and kidney tissues, including neutrophil infiltration in all groups and the presence of macrophages and mononuclear cells in the renal interstitium.</p><p><strong>Conclusion: </strong>Although oat protein supplementation improved lipid profile parameters, prolonged high intake was associated with hematological alterations and transient renal changes. These results highlight the need for caution in the use of high-protein diets based on plant sources and underscore the importance of further studies to clarify their long-term safety. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149238763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cereal-legume intercropping for climate-smart livestock production: insights from oat-alfalfa intercropping.","authors":"Ashraf Alkhtib, Metekia Tamiru, Jane Wamatu","doi":"10.1002/jsfa.71184","DOIUrl":"https://doi.org/10.1002/jsfa.71184","url":null,"abstract":"<p><strong>Background: </strong>Cereal-legume intercropping can improve forage productivity and quality at the same time as enhancing soil function and reducing environmental impacts, yet integrative evaluations under low-input conditions are scarce. This study assessed oat (Avena sativa L.)-alfalfa (Medicago sativa L.) intercropping for forage yield, nutritive value, soil properties and greenhouse gas emission intensity. A randomized complete block design compared sole oat (80 kg ha^-1), sole alfalfa (15 kg ha^-1) and oat-alfalfa seed ratios of 3:1, 1:1 and 1:3 without irrigation or fertilisation. Previous intercropping studies examined agronomic productivity and forage quality separately. The current study addresses the gap with an integrated assessment of forage yield, conservation quality, soil health, greenhouse gas emission intensity, livestock production potential and decision metrics of oat-alfalfa under low-input conditions.</p><p><strong>Results: </strong>Intercropping increased total forage dry matter yield (by 21%), crude protein and metabolisable energy content, and also reduced neutral detergent fibre, compared to sole oat (P < 0.05). The 1:1 intercrop had significantly the highest land equivalent ratio (1.56) and system productivity index and showed better silage fermentation and dry matter recovery (P < 0.05). At final sampling, intercropping increased soil organic carbon, total nitrogen, cation exchange capacity and available phosphorus, with a small reduction in soil pH (P < 0.05). Predicted global warming potential per unit of potential liveweight gain was lower (37-47%) for intercrops than for monocultures for both hay and silage. Multi-criteria decision analysis consistently ranked the 1:1 intercrop highest across producer-centric, balanced and environment-centric scenarios.</p><p><strong>Conclusion: </strong>Oat-alfalfa intercropping, particularly at a 1:1 seed ratio, provides a promising climate-smart forage production strategy by simultaneously enhancing productivity, forage quality and soil fertility at the same time as reducing predicted greenhouse gas emission intensity under low-input conditions. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149238721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of different ultrasonication strategies on the structural, rheological, and functional properties of mung bean protein isolate-gum arabic conjugates formed via the Maillard reaction.","authors":"Halil İbrahim Odabaş, Esra Karabulut, Asena Türk, Merve Tuğçe Tunç, Ceren Elmacı, Nevzat Konar, İlyas Atalar","doi":"10.1002/jsfa.71163","DOIUrl":"https://doi.org/10.1002/jsfa.71163","url":null,"abstract":"<p><strong>Background: </strong>The impact of different ultrasound (US) strategies on the formation of mung bean protein isolate-gum arabic (MBPI-GA) conjugates formed by the Maillard reaction was investigated to overcome the techno-functional limitations of native mung bean protein isolate (MBPI). Two primary sonication strategies - pre-sonication of protein (P100, P200) and co-sonication of the protein-polysaccharide mixture (PG100, PG200) - were compared with traditional wet-heating controls.</p><p><strong>Results: </strong>High-intensity ultrasound (200 W) significantly accelerated Maillard reaction kinetics, resulting in the highest degrees of grafting (44% to 47%) compared with wet-heated MBPI-GA conjugates without ultrasound treatment. Fourier transform infrared (FTIR) spectroscopy and reactive sulfhydryl group analyses confirmed that ultrasonic cavitation induced structural unfolding, as evidenced by a significant reduction in <math><mrow><mi>β</mi></mrow> </math> -sheet fractions and a concomitant increase in random coil content, exposing buried reactive sites. All sonicated conjugates exhibited more than a twofold increase in protein solubility compared with the native protein. The PG200 strategy maximized emulsifying activity index (EAI) and water-holding capacity (8.5 g g^-1), and PG100 yielded the highest in vitro protein digestibility (approximately 60%) and improved thermal stability. The denaturation temperature ( <math> <mrow><msub><mi>T</mi> <mi>d</mi></msub> </mrow> </math> ) increased from 81.32 °C (native MBPI) to 82.65-83.63 °C in the co-sonicated conjugates, accompanied by a pronounced reduction in denaturation enthalpy ( <math><mrow><mi>Δ</mi> <mi>H</mi></mrow> </math> ) from 15.7 to 5.5 J g^-1. Rheological assessments revealed that the conjugates formed a heat-stable, liquid-like network in which the loss modulus ( <math> <mrow><msup><mi>G</mi> <mrow><mo>″</mo></mrow> </msup> </mrow> </math> ) consistently exceeded the storage modulus ( <math> <mrow><msup><mi>G</mi> <mo>'</mo></msup> </mrow> </math> ), thereby preventing thermal gelation and precipitation during processing.</p><p><strong>Conclusion: </strong>These findings suggest that co-sonication of MBPI-GA mixtures is a highly effective green modification strategy for producing homogeneous, functional, and highly digestible plant-based biomacromolecules. The resulting conjugates show considerable potential for use in liquid food formulations and high-temperature industrial processes. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149231121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malodorous compound production driven by microbial succession during Chinese cabbage decomposition.","authors":"Wenyi Deng, Yuqian Yang, Tao Wang, Chuanqi Chu, Shengbao Cai, Junjie Yi, Zhijia Liu","doi":"10.1002/jsfa.71120","DOIUrl":"https://doi.org/10.1002/jsfa.71120","url":null,"abstract":"<p><strong>Background: </strong>Chinese cabbage is one of the most widely consumed vegetables in China. However, malodorous compounds generated from the decomposition of discarded leaves during harvest and distribution have become a significant environmental concern. This study aimed to identify the key odor compounds produced during Chinese cabbage decompostion and to characterize their associations with microbial community dynamics.</p><p><strong>Results: </strong>Physicochemical monitoring showed declining total solids and sugars, rising soluble protein, and fluctuating total nitrogen. Gas chromatography-olfactometry-mass spectrometry (GC-O-MS) identified dimethyl disulfide and dimethyl trisulfide as early dominant odorants. The content of dimethyl disulfide increased from 0.53 to 59.29 μg/kg by day 3, while dimethyl trisulfide peaked at 14.08 μg/kg on day 3 and then became undetectable. Indole accumulated to 9.82 μg/kg by day 5 and persisted thereafter, becoming the major malodorous compound in mid-to-late stages. Full-length 16S/ITS sequencing revealed distinct bacterial and fungal succession, with Alcaligenes faecalis dominating (42.25%) at day 11 and Geotrichum silvicola and Candida tropicalis prevailing among fungi. Spearman correlation suggested associations between specific taxa and key odorants. Screening and validation confirmed that Rhodotorula mucilaginosa and Yarrowia lipolytica removed > 99.7% of indole.</p><p><strong>Conclusion: </strong>Dimethyl disulfide, dimethyl trisulfide, and indole are the principal odor compounds during Chinese cabbage decomposition, with indole dominating the later stages. Microbial succession drives the temporal shift in odor profiles. Rhodotorula mucilaginosa and Yarrowia lipolytica were validated as highly effective indole-degrading agents, highlighting their potential for biological odor mitigation during cabbage decomposition, although their capacity for sulfur-compound removal remains limited. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149228096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive index framework for assessing waterlogging risk in winter wheat across key growth stages in China's main cropping regions.","authors":"Dongxiao Zheng, Dekun Yan, Yuhan Wang, Jin Zhao, Changying Xue, Ying Li, Xiaoguang Yang","doi":"10.1002/jsfa.71165","DOIUrl":"https://doi.org/10.1002/jsfa.71165","url":null,"abstract":"<p><strong>Background: </strong>Climate change has intensified extreme precipitation events, increasing waterlogging risks that threaten agricultural productivity, particularly in China's main winter wheat-producing regions. This study developed a comprehensive framework for assessing waterlogging risk by integrating three dimensions: meteorological drivers (a cumulative wetness index derived from precipitation and crop evapotranspiration), environmental susceptibility (derived from soil texture, slope, and topography), and agricultural exposure (winter wheat cultivation area).</p><p><strong>Results: </strong>During the green-up to heading stage, the proportion of station-years affected by slight waterlogging disasters was 17.9%, that by moderate disasters was 16.8%, and that by severe disasters was 13.6%. During the heading to maturity stage, this proportion decreased to 14.3% for slight waterlogging disasters, to 7.0% for moderate disasters, and to 1.4% for severe disasters. Meteorological risk exhibited a spatial gradient from northwest to southeast during the earlier stage and decreased markedly in the later stage, except in southern Anhui. Areas of high to extremely high comprehensive risk were identified in southern and central Anhui, central-eastern Hubei, southern and eastern Jiangsu, and southern Henan during the green-up to heading stage, with significant reductions during the subsequent stage.</p><p><strong>Conclusion: </strong>Waterlogging risk in the study area was highly stage-dependent and spatially heterogeneous, with the green-up to heading stage identified as the most vulnerable period. The southern provinces faced substantially greater waterlogging threats than the northern wheat-producing regions. These findings provide vital insights for targeted disaster mitigation and climate adaptation strategies in winter wheat production. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shelf-life stability, proximate composition, fatty acid profile, and safety of organic and conventional shelled hazelnuts.","authors":"Sümeyye Şahin, Özlem Balakar Saç","doi":"10.1002/jsfa.71175","DOIUrl":"https://doi.org/10.1002/jsfa.71175","url":null,"abstract":"<p><strong>Background: </strong>Organic foods are increasingly preferred for their perceived health benefits, boosting demand for organically grown hazelnuts. However, information regarding the comparative storage stability of organic and conventional hazelnuts remains limited. This study evaluated the 12-month storage stability of organic shelled hazelnuts (OrgH) and conventional shelled hazelnuts (ConH) under vacuum packaging at room temperature in the absence of light. Before storage, pesticide residue analysis was performed, and no residues were detected in either group.</p><p><strong>Results: </strong>At baseline, OrgH exhibited higher oil content and lower moisture, whereas ConH contained higher protein, ash, linoleic acid, volatile organic compounds (VOCs), and higher L* and b* color values. No significant differences were observed in free fatty acids (FFAs), peroxide value (PV), aflatoxins, total antioxidant capacity (TAC), total phenolic content (TPC), palmitic, stearic, and oleic acids. During storage, PV and FFAs increased in both groups, while most other parameters remained relatively stable. After 12 months, OrgH and ConH were similar (P > 0.05) in oil, protein, moisture, ash, aflatoxin, PV, TAC, and stearic acid levels. ConH maintained higher FFA, L* value, TPC, and linoleic acid, whereas OrgH retained higher VOCs, palmitic and oleic acids, a* and b* color values. Notably, OrgH showed a 1.2-fold lower final FFAs compared to ConH.</p><p><strong>Conclusion: </strong>Organic cultivation did not adversely affect hazelnut shelf life. Both production systems demonstrated comparable storage stability, with organic hazelnuts showing certain quality advantages, particularly lower final FFA levels. These findings indicate that organic hazelnuts can maintain storage quality comparable to conventional hazelnuts while offering specific quality advantages. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interfacial assembly of propylene glycol alginate-modulated dual-protein nanoparticles in alcohol-containing Pickering emulsions: role of oil volume fraction in β-carotene protection.","authors":"Yuan Wang, Jingying Xie, Xinmeng Zhang, Jinfang Liu, Yanxiang Gao","doi":"10.1002/jsfa.71183","DOIUrl":"https://doi.org/10.1002/jsfa.71183","url":null,"abstract":"<p><strong>Background: </strong>Alcohol-containing emulsions face severe colloidal instability because ethanol perturbs biopolymer conformation and interfacial organization. To overcome this, we fabricated ethanol-tolerant Pickering emulsions stabilized via the interfacial assembly of propylene glycol alginate (PGA)-modulated lactoferrin/zein (LZ) composite nanoparticles. This study systematically elucidated the role of the oil-phase volume fraction (φ_oil, 200-700 mL L^-1) in regulating the physicochemical properties, rheological behavior, and β-carotene protection of these complex colloidal systems in an aqueous medium containing 150 mL L^-1 ethanol.</p><p><strong>Results: </strong>The LZ-PGA particles formed particle-rich interfacial layers, conferring solid-like and thermally stable viscoelastic behavior. Increasing the oil fraction promoted droplet crowding and colloidal network formation, whereas excessive oil loading at 700 mL L^-1 promoted droplet destabilization. The emulsion containing 600 mL L^-1 oil showed the best physical stability and β-carotene protection, with enhanced resistance to thermal, ionic, photolytic, and storage stresses. This formulation reduced the photodegradation rate constant to 0.155 h^-1 and extended the β-carotene half-life to 4.42 h, compared with 1.42 h for free β-carotene. During simulated digestion, β-carotene showed a biphasic release profile, and the highest bioaccessibility of 16.11% was achieved at 500 mL L^-1 oil.</p><p><strong>Conclusion: </strong>Oil fraction regulation provides a practical strategy to balance emulsion stability and bioactive delivery. The emulsion containing 600 mL L^-1 oil is preferred for shelf stability and β-carotene protection, whereas that containing 500 mL L^-1 oil is more suitable for digestive release and bioaccessibility. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoencapsulation of phycocyanin peptides enhances anti-inflammatory activity and alleviates ulcerative colitis.","authors":"Zongyu Zhang, Shuang Du, Yunlong Chen, Jing Dai, Guojing Xu, Hailin Yin, Liangtao Lv","doi":"10.1002/jsfa.71187","DOIUrl":"https://doi.org/10.1002/jsfa.71187","url":null,"abstract":"<p><strong>Background: </strong>Ulcerative colitis (UC) is a prevalent inflammatory bowel disease, and current medications have considerable side effects. Phycocyanin peptides (PCPs) have demonstrated anti-inflammatory potential; however, their clinical application is limited by poor stability and low bioavailability. This study prepared highly active PCPs via ultrasound-assisted enzymatic hydrolysis and used chitosan-sodium tripolyphosphate nanoencapsulation to improve their application performance.</p><p><strong>Results: </strong>PCPs significantly inhibited nitric oxide release in lipopolysaccharide-induced RAW264.7 cells. The encapsulation efficiency of phycocyanin peptide nanoparticles (PCP-NPs) reached 91.33%, with markedly enhanced gastrointestinal stability and bioavailability. In mouse experiments, PCP-NPs reduced the disease activity index of ulcerative colitis mice, repaired colonic injury, down-regulated pro-inflammatory cytokines, and up-regulated anti-inflammatory cytokines and intestinal tight junction proteins. Three core anti-inflammatory peptides, YAINA, FPYTT, and YVTYA, were isolated and identified.</p><p><strong>Conclusion: </strong>Nanoencapsulation strengthens the anti-inflammatory activity and intestinal barrier repair capacity of PCPs, which hold promising application potential as functional food ingredients for ulcerative colitis prevention. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of drying methods on polyphenolic composition and anti-inflammatory activity of Lilium lancifolium leaves and stems.","authors":"Peiyao Yu, Baoxin Zhang, Jia Hu, Hong Quan, Jiang Liu, Xiaozhong Lan, Xinbo Guo","doi":"10.1002/jsfa.71173","DOIUrl":"https://doi.org/10.1002/jsfa.71173","url":null,"abstract":"<p><strong>Background: </strong>Lilium lancifolium Thunb. (tiger lily) is widely utilized in East Asia for its medicinal and edible bulb, whereas its aerial parts (leaves and stems) are largely discarded despite containing potentially valuable polyphenolic constituents. This study investigated how different drying methods affect the polyphenolic composition and anti-inflammatory activity, and explored associated transcriptional responses related to inflammation.</p><p><strong>Results: </strong>Eight target polyphenols were detected and quantified across leaf and stem samples using high-performance liquid chromatography, with regaloside A and regaloside C as the dominant constituents. Drying methods markedly influenced polyphenol stability: vacuum drying increased the summed concentration of quantified polyphenols in leaves and partially preserved these constituents in stems, whereas hot-air and natural-air drying caused substantial degradation. In a lipopolysaccharide-induced zebrafish inflammation model, stem extracts from fresh and vacuum-dried samples exhibited the strongest inhibition of NO and pro-inflammatory cytokines (TNF-α, IL-6, IL-1β). For leaves, the response varied by inflammatory endpoint: fresh leaf extract showed the greatest reductions in NO and TNF-α, whereas IL-6 and IL-10 did not differ significantly among the extract-treated groups. RT-qPCR analysis demonstrated that fresh stem and leaf extracts downregulated pro-inflammatory gene expression while upregulating il10, with higher concentrations showing transcriptional changes consistent with attenuation of NF-κB/MAPK-associated inflammatory responses.</p><p><strong>Conclusion: </strong>Drying methods profoundly influence the chemical composition and bioactivity of L. lancifolium aerial parts. Vacuum drying showed tissue-dependent advantages in polyphenol preservation, while inflammation-related activity varied according to tissue and endpoint. These findings highlight the potential of leaves and stems as sustainable resources for developing natural anti-inflammatory agents and promote their high-value utilization. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profiling and pharmacological evaluation of Lablab purpureus seed coat: a rich source of antioxidant, antibacterial and anticancer metabolites.","authors":"Md Rabiul Hossain, Rony Shill, Md Amzad Hasan, Samira Anam, Nadia Islam Chowdhury, Rukeya Yesmin Mysha, Md Shafayet Hossain, Fatiha Sultana Fiha, Md Abdul Motaleb Bhuiya, Atm Yusuf","doi":"10.1002/jsfa.71151","DOIUrl":"https://doi.org/10.1002/jsfa.71151","url":null,"abstract":"<p><strong>Background: </strong>Cancer remains a major health concern worldwide, highlighting the need for safer and affordable treatments from natural sources. This study evaluated the antioxidant, antibacterial and anticancer potential of different solvent fractions of Lablab purpureus seed coat using in vitro, in vivo and in silico approaches. Methanol, ethyl acetate, dichloromethane and n-hexane extracts were subjected to phytochemical screening, antioxidant assays, cytotoxicity testing against HeLa cells, antibacterial evaluation, molecular docking and molecular dynamics studies targeting estrogen receptor alpha.</p><p><strong>Results: </strong>Among the tested extracts, the methanolic fraction showed the strongest antioxidant activity, with half-maximal inhibitory concentration values of 86.83 μg mL^-1 and 67.56 μg mL^-1 in the 2,2-diphenyl-1-picrylhydrazyl (i.e. DPPH) and 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (i.e. ABTS) assays, respectively. This activity was consistent with its high phenolic and flavonoid contents. In a carbon tetrachloride-induced oxidative stress mouse model, the methanolic extract significantly improved serum glutamate pyruvate transaminase, serum glutamate oxaloacetate transaminase and catalase levels. It also demonstrated strong cytotoxic activity against HeLa cells, reducing cell viability by more than 95%. The n-hexane extract showed notable antibacterial activity, particularly against Pseudomonas aeruginosa, with a zone of inhibition of 32 mm. Gas chromatography-tandem mass spectrometry analysis of the methanolic crude extract identified 76 compounds, among which N-benzylisatoic anhydride and pallensin showed strong binding affinity toward estrogen receptor alpha, with docking scores comparable to doxorubicin. Pallensin also exhibited favorable electronic properties, whereas molecular dynamics, molecular mechanics/generalized born surface area and principal component analyses confirmed stable ligand-receptor interactions.</p><p><strong>Conclusion: </strong>Overall, the findings indicate that L. purpureus seed coat is a promising source of bioactive compounds with antioxidant, antibacterial and anticancer activities. © 2026 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149224324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}