黄酮类化合物提取工艺的优化[j]。[Osbeck]用超临界CO2和乙醇处理果皮：化学成分、抗氧化和抗菌活性。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-08 DOI:10.1002/jsfa.70136

Jielin Luo, Yaokun Pang, Dan Liu, Jianxia Sun, Weibin Bai

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引用次数: 0

摘要

背景：血橙皮是一种未被充分利用的具有广泛生物活性的高价值黄酮类化合物来源。传统的柑橘类黄酮单次提取工艺由于极性限制，提取效率低，严重制约了柑橘类黄酮的产业化开发和应用。因此，迫切需要开发绿色高效的提取工艺，以提高柑橘类黄酮的提取率和提取率。结果：本研究建立了两步联合提取血橙皮黄酮的工艺：超临界CO2萃取(SCE) -残乙醇萃取（REE）。采用Box-Behnken设计响应面法对关键工艺参数进行优化，以达到提取效率最大化。与传统溶剂萃取相比，该工艺具有以下优势：(1)通过超高效液相色谱-四极杆-轨道阱高分辨率质谱联用定性分析，鉴定出两步萃取物中多出7种黄酮类化合物，表明SCE主要由低极性黄酮类化合物组成，REE主要由高极性黄酮类化合物组成；(2) HPLC定量分析表明，两步提取可显著提高黄酮类化合物含量，总黄酮含量提高11.75%，多甲氧基黄酮类化合物含量提高19.55%；(3)抗氧化和抗菌分析表明，REE提取物具有较强的抗氧化能力，SCE提取物具有较强的抗菌活性。结论：本研究创新开发了一种两步联合提取工艺，可有效地逐步富集血橙皮中不同极性的黄酮，显著提高血橙皮中总黄酮和多甲氧基黄酮的含量。该研究首次阐明了低极性SCE和高极性REE提取物之间的极性互补性，为定向利用黄酮类化合物提供了新的策略。©2025化学工业协会。

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Optimization of flavonoid extraction from Tarocco orange (Citrus sinensis [L.] Osbeck) peel using supercritical CO₂ followed by ethanol: chemical composition, antioxidant, and antibacterial activities.

Background: Blood orange peels represent an underutilized source of high-value flavonoids with broad bioactivities. Traditional single-extraction techniques for citrus flavonoids suffer from low efficiency due to polarity limitations, significantly restricting their industrial-scale development and application. As a result, there is an urgent need to develop green and efficient extraction processes to improve both the coverage and yield of citrus flavonoids.

Results: This study developed a two-step combined extraction process to obtain flavonoids from blood orange peels: supercritical CO₂ extraction (SCE) followed by residue ethanol extraction (REE). Response surface methodology with Box-Behnken design was employed to optimize key process parameters for maximizing extraction efficiency. Compared to traditional solvent extraction, this process exhibited the following advantages: (1) qualitative analysis by ultra-performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry identified seven additional flavonoids in the two-step extract, revealing that SCE was primarily composed of low-polarity flavonoids, and REE was primarily composed of high-polarity flavonoids; (2) quantitative analysis by HPLC demonstrated that the two-step extraction significantly enhanced flavonoid content, with total flavonoids increasing by 11.75% and polymethoxyflavones rising by 19.55%; (3) antioxidant and antimicrobial analyses revealed the potent antioxidant capacity of REE extracts and the superior antimicrobial activity of SCE extracts.

Conclusion: This study innovatively developed a two-step combined extraction process for efficient stepwise enrichment of flavonoids with varying polarities from blood orange peel, significantly increasing the content of total flavonoids and polymethoxyflavones. For the first time, it elucidated the polarity complementarity between low-polarity SCE and high-polarity REE extracts, providing a novel strategy for polarity-targeted flavonoid utilization. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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