Journal of the Science of Food and Agriculture最新文献

{"title":"Spore display innovation technology for unlocking new substrates for glucose oxidase.","authors":"Xihua Chen, Zeyang Li, Zhengfen Wu, Yong Feng, Yong Chen, Yi Ma, Zhongjian Guo, Huayou Chen","doi":"10.1002/jsfa.14420","DOIUrl":"https://doi.org/10.1002/jsfa.14420","url":null,"abstract":"<p><strong>Background: </strong>Glucose oxidase (GOD) has been extensively applied in the animal feed industry. However, its substrate specificity limits its utility in the broad degradation of fibrous raw materials.</p><p><strong>Results: </strong>In this study, a series of spore display technologies were employed to transform GOD from an enzyme specific for β-d-glucose into a broad-spectrum sugar oxidase capable of catalyzing various sugars such as arabinose, xylose, sucrose, cellobiose, raffinose, and sorbitol. Notably, the relative enzyme activities for arabinose, xylose, and sorbitol increased from less than 5% to 71%, 52%, and 53%, respectively. Furthermore, the optimal temperature for GOD activity shifted from 30 °C to 60 °C, significantly improving its thermal stability.</p><p><strong>Conclusion: </strong>Enzymes with high activity, strong heat resistance, and the ability to catalyze various sugars can help reduce the accumulation of hydrolysis products in animal feed, improving the cost-effectiveness of the feed. This contributes to the advancement of sustainable agriculture and the further development of feed production. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of polyphenols on physicochemical and digestive properties of Trichosanthes kirilowii starch.","authors":"Yubo Ma, Peixuan Du, Yongkang Sun","doi":"10.1002/jsfa.14440","DOIUrl":"https://doi.org/10.1002/jsfa.14440","url":null,"abstract":"<p><strong>Background: </strong>As a natural functional ingredient, Trichosanthes kirilowii (TK) starch is widely used in the fields of food and medicine, but its physicochemical stability and digestive properties still need to be optimized to enhance its application potential. Polyphenolic substances (such as ferulic acid, caffeic acid, etc.) have attracted much attention because of their ability to improve food functional properties and regulate digestion and metabolism. The present study focused on the co gelation of polyphenols and TK starch, aiming to systematically reveal the regulation mechanism of five polyphenols (ferulic acid, caffeic acid, catechin, gallic acid and quercetin) on the physicochemical properties and digestion behavior of starch, and also provide a theoretical basis for the development of functional starch based composites.</p><p><strong>Results: </strong>Polyphenols increased the solubility and swelling power of starch and enhanced the freeze-thaw stability. X-ray diffraction analysis has shown that starch and polyphenols form a V-shaped complexes, the crystallinity increased. According to the Fourier transform infrared spectroscopy results, polyphenols established no new characteristic peaks of starch, but adding polyphenols helped improve the ordered structure of starch. As measured by a rapid viscosity analyzer, polyphenols decreased the viscosity of starch, improved thermal stability and effectively delayed the aging of starch. The results of the in vitro digestion test demonstrated that the content of rapidly digested starch in the complex decreased, whereas the content of resistant starch increased, helpful in delaying the digestion process of starch powder.</p><p><strong>Conclusion: </strong>Five polyphenols can effectively regulate the physical and chemical properties of the TK starch through co gelation, enhance its stability and improve its digestive characteristics, which may have potential health benefits (such as delaying blood glucose response). The research results provide a theoretical basis for the application of polyphenol starch complexes. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic inhibition of α-glucosidase and α-amylase by phenolic compounds isolated from olive oil by-products.","authors":"Fátima Rubio-Senent, Alejandra Bermúdez-Oria, Antonio Lama-Muñoz, Guillermo Rodríguez-Gutiérrez, Juan Fernández-Bolaños","doi":"10.1002/jsfa.14424","DOIUrl":"https://doi.org/10.1002/jsfa.14424","url":null,"abstract":"<p><strong>Background: </strong>The inhibition of carbohydrate-digesting enzymes, α-glucosidase and α-amylase, is a key strategy in managing postprandial hyperglycemia in type 2 diabetes. Natural phenolic compounds isolated from olive mill by-product (alperujo), such as hydroxytyrosol (HT) and trans-p-coumaroyl secologanoside (comselogoside, COM), have shown potential in this regard. However, the individual effect of the 3,4-dihydroxyphenylglycol (DHPG), another potent phenolic antioxidant naturally found in olive fruit with the same ortho-diphenolic structure as HT, but with an additional hydroxyl group in the β position, and their combination with HT and COM on these enzymes remain underexplored.</p><p><strong>Results: </strong>The present study evaluated the inhibitory effects of HT, DHPG and COM on α-glucosidase and α-amylase. DHPG exhibited the strongest inhibition of α-glucosidase (IC₅₀ = 288.2 μm), comparable to the anti-diabetic drug acarbose (IC₅₀ = 281.9 μm), whereas HT and COM showed moderate activity. Binary combinations of HT and DHPG (1:1) displayed synergistic inhibition of both enzymes, significantly enhancing their efficacy. Conversely, DHPG and COM combinations resulted in antagonistic effects on α-amylase inhibition, although they showed a significant inhibitory effect on α-glucosidase (IC₅₀ = 100.7 μm).</p><p><strong>Conclusion: </strong>These findings highlight the potential of phenolic compounds derived from olive oil by-products as natural alternatives for postprandial glucose control. The synergistic interactions between specific compounds suggest an enhanced therapeutic effect that could be used for diabetes management. Further studies are needed to elucidate the mechanisms underlying these interactions and assess their in vivo efficacy. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization on the physicochemical and digestion properties of potato starch modified by the extract from Rhinacanthus nasutus (L.) KURZ.","authors":"Junyu Liu, Shuqin Li, Jingyang Lu, Mingyue Li, Min Zhang, Pharkphoom Panichayupakaranant, Haixia Chen","doi":"10.1002/jsfa.14407","DOIUrl":"https://doi.org/10.1002/jsfa.14407","url":null,"abstract":"<p><strong>Background: </strong>Rhinacanthus nasutus (L.) KURZ (R. nasutus), a tropical functional tea, has a wide range of hypoglycemic applications. The slow digestion of potato starch is important for the stability of glucose spikes. However, the effects of R. nasutus on the physicochemical and digestive properties of potato starch are unknown.</p><p><strong>Results: </strong>Q Exactive HF liquid chromatography/mass spectrometry analysis showed that R. nasutus extract (RNE) contained naphthoquinones, fatty acids, terpenoids and others. RNE decreased the solubility and swelling of potato starch. The starch granuloid transformation and crosslinking were detected by scanning electon microscopy. The crystallinity of the starch changed obviously, the texture properties were more hardened and the viscosity was stronger. With the addition of RNE (8 g kg^-1), the digestion of starch in vitro was significantly inhibited with the proportions of fast digestible starch decreasing to 16.53% and the proportion of slow digestible starch increasing to 34.76%. IEC-6 cell assays confirmed the safety of RNE. Molecular docking results showed that RNE could interact with the amino acid residues of the digestive enzymes through hydrogen bonds, alkyl and pi-alkyl interactions, which could reduce the digestive rate of starch.</p><p><strong>Conclusion: </strong>RNE was found to modify the physicochemical properties of potato starch, decreasing the solubility and swelling degree, hardening the structural properties, and increasing the viscosity, which are helpful for the edible properties of starch. The addition of RNE significantly inhibited the digestion of starch in vitro. R. nasutus and its extract might be potential candidates for new food additives. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of plant polysaccharides on intestinal health via targeted regulation of gut microbiota.","authors":"Yourong Ye, Licuo Ze, Mengqi Duan, Zhankun Tan, Yushi Wang, Hui Zhang, Peng Shang","doi":"10.1002/jsfa.14417","DOIUrl":"https://doi.org/10.1002/jsfa.14417","url":null,"abstract":"<p><p>Plant polysaccharides constitute a pivotal class of bioactive compounds that significantly impact intestinal health and immune function. Extensive research has highlighted their capacity to enhance intestinal mucosal barrier function, improve intestinal immunity and mitigate inflammation. This review comprehensively delves into the positive impacts of plant polysaccharides on intestinal health, with particular emphasis on their ability to modulate the interaction between gut microbiota and intestinal T helper cells, inhibit inflammatory cytokines, regulate inflammatory pathways, combat oxidative stress and modulate metabolic pathways. As natural bioactive substances, plant polysaccharides play a pivotal role in regulating intestinal health and immune function, presenting novel avenues for disease prevention and treatment. Despite the recognized protective effects of plant polysaccharides on gut microbiota, current research is constrained by methodological limitations and a lack of exploration into their mechanisms of action. However, with the advancement of scientific research methods and the ongoing development of clinical applications, it is anticipated that future studies will yield deeper insights into their protective effects and broaden their clinical applications. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing β-carotene bioavailability and protective effects for dextran sodium sulfate-induced colitis with sesame protein hydrolysate nanoparticles.","authors":"Dounya Zad Oumeddour, Xin Ai, Yaqi Liu, Yutong Yuan, Zehui Zhu, Mingxin Zhang, Lei Zhao, Liang Zhao","doi":"10.1002/jsfa.14438","DOIUrl":"https://doi.org/10.1002/jsfa.14438","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory bowel disease (IBD) is a chronic condition marked by recurring intestinal inflammation, leading to symptoms such as fatigue, diarrhea, and weight loss. Current treatments are limited, highlighting the need for new therapeutic strategies. β-Carotene (BC), a natural antioxidant, has potential in managing colitis, but its low bioavailability limits its efficacy. This study evaluated a novel BC-loaded sesame protein hydrolysate nanoparticles (BC-SPH NPs) formulation to enhance BC bioavailability and therapeutic performance in a dextran sodium sulfate (DSS)-induced colitis model.</p><p><strong>Results: </strong>BC-SPH NPs showed an average size of 50.30 ± 0.43 nm, an encapsulation efficiency of 75.23 ± 0.92%, a prolonged plasma residence profile, with a time to reach maximum plasma concentration (T_max) of 8 h, and a peak plasma concentration of 64.982 ng mL^-1. Notably, the NPs enabled enhanced tissue distribution of BC to key tissues in rats including kidneys, liver, small intestine, and colon. In DSS-induced colitis mice, BC-SPH NPs significantly reduced disease activity index, improved body weight, preserved colon length and structure, and prevented inflammatory cell infiltration. Additionally, BC-SPH NPs lowered serum interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor alpha (TNF-α), as well as colonic myeloperoxidase (MPO) activity and nitric oxide (NO) levels. Furthermore, BC-SPH NPs up-regulated tight junction proteins Claudin-1, ZO-1, and Occludin, indicating improved intestinal barrier integrity. In contrast, free BC provided only modest effects.</p><p><strong>Conclusion: </strong>This study demonstrates that BC-SPH NPs substantially enhance the bioavailability and therapeutic effects of BC in DSS-induced colitis compared to free BC. The nanoformulation effectively alleviates colitis symptoms, preserves intestinal integrity, and reduces inflammation, suggesting that BC-SPH NPs represent a promising natural therapeutic option for the prevention and treatment of ulcerative colitis. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potato post-dormancy sprouting: a problem during storage and its control by essential oils as alternatives to artifact chemicals - a review.","authors":"Umesh Chandra Lohani, Rajpreet Kaur Goraya, Saipriya Ramalingam, Thiruppathi Senthilkumar, Chandra B Singh","doi":"10.1002/jsfa.14419","DOIUrl":"https://doi.org/10.1002/jsfa.14419","url":null,"abstract":"<p><p>Potato (Solanum tuberosum L.) is one of the staple food crops grown in most of the world. Potato being one of the major crops in Canada, potato processing is of utmost interest to the food industry. Prior to processing, storage of tubers is an essential step for the potato processing industries. However, the sprouting of potatoes during storage is a crucial problem that results in increased weight loss, respiration, decreased nutritional quality and eventually food waste and economic loss. Tuber dormancy and sprouting are significantly affected by potato cultivar, light, storage temperature, humidity, storage ambience and so forth. Conventional and novel techniques to suppress sprouting have been evolved based on artificial chemical, synthetic, physical and natural chemical suppressants. This review describes the effect of various storage parameters on potato sprouting and the role of suppressants along with basic storage parameters to augment their effect on potato sprouting. Further, this review describes the drawbacks of artificial chemical and synthetic sprouting suppressants, and the role of natural chemical suppressants in overcoming the said drawbacks. Moreover, limitations and research gaps of natural chemical suppressants are discussed highlighting efforts to develop novel approaches followed by the restrictions and problems of natural chemical suppressants for their greater efficacy. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humic acid, biochar-based microbial agent and vermicompost improve cotton growth, photosynthesis, yield and fiber quality in saline-alkali soil.","authors":"Ge Li, Yuyang Shan, Yungang Bai, Weibo Nie, Yan Sun, Lijun Su, Jianghui Zhang, Hongbo Liu, Yu Ding, Xiaoyan Wang, Hongqin Lu, Jinhao Ye, Lei Gao","doi":"10.1002/jsfa.14409","DOIUrl":"https://doi.org/10.1002/jsfa.14409","url":null,"abstract":"<p><strong>Background: </strong>Humic acid (HA), biochar-based microbial agent (M) and vermicompost (V) have been shown to improve soil structure and increase crop yields. However, the combined effects of these amendments on cotton growth, photosynthetic characteristics, yield and fiber quality in saline-alkali soils remain unclear.</p><p><strong>Results: </strong>The results obtained in the present study showed that: (i) compared to the control (CK), the single and combined applications of HA, M and V increased soil water storage and reduced soil salinity; (ii) the combined application of HA, M and V resulted in the greatest improvements in plant height, stem diameter, leaf area index, net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, transpiration rate, SPAD, biomass and yield, with increases of 39.2%, 36.0%, 88.1%, 43.9%, 97.8%, 41.1%, 133.9%, 18.0%, 85.1% and 31.8%, respectively; (iii) the optimal application rates of HA, M and V were 120, 60 and 120 kg ha^-1, respectively; (iv) correlation analysis and structural equation modeling revealed that cotton yield was positively correlated with biomass, growth indices and photosynthetic characteristics, whereas fiber quality was positively correlated with photosynthetic characteristics, soil water storage and yield.</p><p><strong>Conclusion: </strong>Based on these findings, the combined application of HA, M and V is recommended to enhance cotton growth in saline-alkali soils. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-enhanced seed hydration: impacts on seedling vigor, gene expression and absorption kinetics in maize, bean and pepper seeds.","authors":"António Nogueira, Hélder Puga, Hernâni Gerós, António Teixeira","doi":"10.1002/jsfa.14426","DOIUrl":"https://doi.org/10.1002/jsfa.14426","url":null,"abstract":"<p><strong>Background: </strong>Various methods have been explored to improve seed hydration, reduce hydration time and improve germination rates, such as seed priming methods and non-invasive technologies like ultrasound. Several reports have highlighted ultrasound technology's positive effects on germination and seedling development by enhancing water absorption. However, a comprehensive study investigating ultrasound's impact on seed water uptake dynamics, gene expression linked to seed hydration, and its association with germination rates has not yet been conducted.</p><p><strong>Results: </strong>The present study tested the contribution of ultrasound technology in enhancing seed hydration of three crop species with high economic value: pepper, maize and bean, its impact on germination and in the expression of genes involved in the germination process. Moreover, scanning electron microscopy and numerical simulation were utilized to evaluate the structural changes in seed coat in response to ultrasound. The study employed a variety of models to analyse hydration kinetics and found that ultrasound treatment consistently improved water uptake in all three studied species. Specifically, seeds exposed to ultrasound showed faster and higher water absorption rates, enhanced seedling growth, and significant modulation of gene expression. The treatment led to overexpression of aquaporin-coding genes and repression of genes involved in the abscisic acid (ABA) biosynthesis.</p><p><strong>Conclusion: </strong>Overall, ultrasound treatment proved to be an effective priming strategy, enhancing seed hydration kinetics, modulating the expression of genes involved in water transport and ABA biosynthesis, and promoting germination and seedling growth, particularly in bean seeds. These findings highlight the potential of ultrasound technology as a sustainable tool to improve seed performance and bring valuable knowledge to the field of seed physiology and agricultural technology. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-destructive quantification of egg yolk ratio using visible-near-infrared hyperspectral imaging, machine learning and explainable AI.","authors":"Md Wadud Ahmed, Jason Lee Emmert, Mohammed Kamruzzaman","doi":"10.1002/jsfa.14431","DOIUrl":"https://doi.org/10.1002/jsfa.14431","url":null,"abstract":"<p><strong>Background: </strong>Accurate, non-destructive quantification of egg yolk ratio holds considerable significance for the food industry, nutritional assessment and egg grading. Conventional approaches are limited by destructive testing and insufficient throughput for commercial applications. This study investigates the potential of visible-near-infrared (Vis-NIR; 374-1015 nm) hyperspectral imaging (HSI) combined with machine learning (ML) and explainable artificial intelligence (AI) techniques for rapid and non-destructive yolk ratio prediction. Multiple regression models, spectral preprocessing and feature selection techniques were comprehensively evaluated to develop robust and interpretable predictive solutions.</p><p><strong>Results: </strong>Regression models including partial least squares regression (PLSR), random forest, extreme gradient boosting and support vector regression were assessed for yolk ratio prediction. The PLSR model combined with Savitzky-Golay first-derivative spectral preprocessing demonstrated superior and stable predictive performance, achieving coefficients of determination (R²) of 0.79, 0.73 and 0.68 in calibration, validation and independent test datasets, respectively. Additionally, a simplified PLSR model using a few important variables selected based on regression coefficients achieved robust predictive results. Shapley additive explanations analysis provided clear insights into the wavelength regions significantly contributing to model predictions, primarily linked to water, lipid and protein contents in eggs.</p><p><strong>Conclusion: </strong>This research highlights the effectiveness of Vis-NIR HSI integrated with ML and explainable AI as a rapid, reliable and non-destructive approach for egg yolk ratio assessment. The developed method offers significant advantages for egg quality monitoring, providing practical, interpretable and scalable solutions beneficial for food industry applications. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}