Journal of the Science of Food and Agriculture最新文献

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Fall-planted cover crops improve nitrogen use efficiency but reduce garlic yield in a relay intercropping system amended with arbuscular mycorrhizal fungi. 在丛枝菌根真菌改良的间作体系中,秋种覆盖作物提高了氮素利用效率,但降低了大蒜产量。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-26 DOI: 10.1002/jsfa.70215
Alexandra Smychkovich, Masoud Hashemi
{"title":"Fall-planted cover crops improve nitrogen use efficiency but reduce garlic yield in a relay intercropping system amended with arbuscular mycorrhizal fungi.","authors":"Alexandra Smychkovich, Masoud Hashemi","doi":"10.1002/jsfa.70215","DOIUrl":"https://doi.org/10.1002/jsfa.70215","url":null,"abstract":"<p><strong>Background: </strong>Integrating fall-planted cover crops (CC) and arbuscular mycorrhizal (AM) fungi into hardneck garlic production can enhance the sustainability of garlic production in the northeast. We hypothesized that relay intercropping (RI) garlic into standing CC and inoculating with commercially available AM fungi would (i) influence garlic bulb yield and nutrient concentration as a result of increased nutrient retention in CC residues and (ii) enhance the nitrogen use efficiency (NUE) of garlic production by providing an alternative N source (decomposing CC residues) to garlic plants in spring.</p><p><strong>Results: </strong>Garlic yield response to RI depended on CC fall biomass accumulation (2301-3696 kg ha<sup>-1</sup> dry weight in 2021, < 1500 kg ha<sup>-1</sup> in 2020). Interspecies competition between CC and garlic reduced bulb yield by 21-31% in RI treatments compared to the no cover crop control in 2021. Although bulb N, P, K and Mn assimilation was negatively impacted in the first year, nutrient concentrations were normalized in the second cropping season, suggesting that multiple years are necessary to improve garlic quality under a RI system. Garlic yield and nutritional quality were not significantly improved by AM fungi inoculation in this study. NUE was optimized under oat RI systems, which had the highest yields among RI treatments in the second year.</p><p><strong>Conclusion: </strong>RI with CC can improve NUE of garlic production systems, but CC should be managed appropriately to avoid reductions in bulb yield. Multi-year implementation of RI balances nutrient assimilation by garlic, which was negatively impacted in the first year of production. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of propolis extracts from the Tolima region in Colombia: a study of their composition, antimicrobial activity, and cytotoxicity. 哥伦比亚托利马地区蜂胶提取物的表征:其组成、抗菌活性和细胞毒性的研究。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-25 DOI: 10.1002/jsfa.70223
July Alexandra Hernandez-Lopez, Brandhon Francisco Flores-Ibarra, Eduart Gutierrez-Pineda, Sergio E Moya, Guillermo Salamanca Grosso, Cristian C Villa
{"title":"Characterization of propolis extracts from the Tolima region in Colombia: a study of their composition, antimicrobial activity, and cytotoxicity.","authors":"July Alexandra Hernandez-Lopez, Brandhon Francisco Flores-Ibarra, Eduart Gutierrez-Pineda, Sergio E Moya, Guillermo Salamanca Grosso, Cristian C Villa","doi":"10.1002/jsfa.70223","DOIUrl":"https://doi.org/10.1002/jsfa.70223","url":null,"abstract":"<p><strong>Background: </strong>Propolis is a complex resinous material collected by bees from plant sources and known for its rich content in bioactive phenolic compounds, including flavonoids and aromatic acids. Its chemical composition and biological activity are highly influenced by the botanical and environmental conditions surrounding the beehives. In Colombia, limited studies have addressed the chemical diversity and therapeutic potential of propolis from regions with high altitudinal variation such as Tolima.</p><p><strong>Results: </strong>In this study, three propolis ethanolic extracts (PrEE1, PrEE2, PrEE3) were obtained from hives located at different altitudes in Tolima, Colombia (1250, 1100, and 900 m above sea level (a.s.l.), respectively). Samples were analyzed for total phenolic content (TPC), total flavonoid content (TFC), antioxidant capacity (DPPH, ABTS, FRAP), antimicrobial activity (disk diffusion and minimum inhibitory concentration), and cytotoxicity in A549, WI-38 and NIH-3 T3 cell lines. Ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis revealed a distinct phytochemical profile for each extract. PrEE1 had the highest TPC (130.2 g GAE kg<sup>-1</sup>) and TFC (6.70 g QE kg<sup>-1</sup>), and strong antioxidant activity with DPPH scavenging of 89.2%. It also exhibited potent antimicrobial activity against Staphylococcus aureus and Candida albicans, and selective cytotoxicity toward A549 lung cancer cells. In contrast, PrEE3 had lower phenolic content but contained unique polyprenylated benzophenones.</p><p><strong>Conclusion: </strong>The results indicate that altitude significantly influences the chemical and biological properties of Colombian propolis. Propolis from higher altitudes, such as PrEE1, is a promising natural source of multifunctional bioactive compounds with antioxidant, antimicrobial, and anticancer potential for pharmaceutical and nutraceutical applications. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced co-production of polysaccharide and phycoerythrin of Porphyridium purpureum via the synergistic effect of glycerol supplementation and increased irradiance in a stirred tank photobioreactor. 在搅拌槽式光生物反应器中,通过添加甘油和增加辐照度的协同作用,促进紫斑卟啉多糖和藻红蛋白的协同生产。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-24 DOI: 10.1002/jsfa.70197
Ai Hua Zhang, Sanjiong Yi, Zijie Zhao, Jianke Huang, Xun Zhi Zhang, Tao Wang, Bo Feng, Liang Ji, Xinghu Zhou, Pan Zhang, Jianhua Fan
{"title":"Enhanced co-production of polysaccharide and phycoerythrin of Porphyridium purpureum via the synergistic effect of glycerol supplementation and increased irradiance in a stirred tank photobioreactor.","authors":"Ai Hua Zhang, Sanjiong Yi, Zijie Zhao, Jianke Huang, Xun Zhi Zhang, Tao Wang, Bo Feng, Liang Ji, Xinghu Zhou, Pan Zhang, Jianhua Fan","doi":"10.1002/jsfa.70197","DOIUrl":"https://doi.org/10.1002/jsfa.70197","url":null,"abstract":"<p><strong>Background: </strong>To achieve efficient co-production of polysaccharides and phycoerythrin of Porphyridium purpureum in a 40 L stirred tank photobioreactor, a strategy of stepwise increasing irradiance and adding glycerol was proposed after optimization of environmental factors for microalgae growth.</p><p><strong>Results: </strong>The results showed that the optimal temperature and NaCl concentration were 25 °C and 25 g L<sup>-1</sup>, respectively. In addition, 100 and 13 μmol m<sup>-2</sup> s<sup>-1</sup> were identified as the optimal incident light intensity and volume-averaged light intensity, respectively. Among the four different culture strategies, compared with the traditional photoautotrophic culture with constant light intensity, the production of biomass, polysaccharides, and phycoerythrin were enhanced by 266.38%, 390.24%, and 61.76% under mixotrophic culture with stepwise increasing light intensity, respectively. The result demonstrated that the combination of glycerol supplementation and increased light intensity had a synergistic effect. Moreover, the production costs of polysaccharide and phycoerythrin decreased by 78.42% and 34.57% compared to the typical culture method (photoautotrophic culture with constant light intensity).</p><p><strong>Conclusion: </strong>The strategy of mixotrophic culture with stepwise increasing light intensity not only enhanced the productivity of biomass and active substances, but also reduced production costs. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Uncovering key bioactive fatty acids from velvet antler extracts that promote healthspan and neuroprotection in Caenorhabditis elegans. 揭示鹿茸提取物中促进秀丽隐杆线虫健康和神经保护的关键生物活性脂肪酸。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-24 DOI: 10.1002/jsfa.70166
Ru Li, Yumin Yao, Ying Liu, Xiaohui Wang, Hongyuan Li, Yanfang Liu, Yinghua Peng
{"title":"Uncovering key bioactive fatty acids from velvet antler extracts that promote healthspan and neuroprotection in Caenorhabditis elegans.","authors":"Ru Li, Yumin Yao, Ying Liu, Xiaohui Wang, Hongyuan Li, Yanfang Liu, Yinghua Peng","doi":"10.1002/jsfa.70166","DOIUrl":"https://doi.org/10.1002/jsfa.70166","url":null,"abstract":"<p><strong>Background: </strong>Velvet antler has been traditionally recognized for its multifaceted health benefits, yet the underlying pharmacological mechanisms have remained poorly understood.</p><p><strong>Results: </strong>In this study, we employed advanced analytical techniques, including liquid chromatography-mass spectrometry and gas chromatography, coupled with a Caenorhabditis elegans model, to elucidate the active small-molecule components and their associated bioactivities. Our results demonstrate that a methanol-derived velvet antler extract significantly extended the lifespan of C. elegans, enhanced physical functions such as pharyngeal pumping and body bends, and protected against toxic protein aggregation in models of Parkinson's disease (α-synuclein), Huntington's disease (polyQ), and Alzheimer's disease (Aβ). Through systematic fractionation and bioactivity-guided assays, we identified stearic acid (C18:0), linoleic acid (C18:2n), and arachidonic acid (C20:4n) as key fatty acids responsible for these health-promoting effects. Notably, a mixture of these three fatty acids at optimal concentrations conferred healthspan benefits and neuroprotection comparable to the complete extract.</p><p><strong>Conclusion: </strong>These findings provide novel insights into the pharmacological potential of velvet antler and highlight specific fatty acids that could serve as promising therapeutic agents for aging and neurodegenerative disorders. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro dynamic infant digestion model: effects of 1,3-dioleoyl-2-palmitoyl glycerol, lactoferrin, and casein phosphopeptide fortification on infant formula protein and fat digestion. 体外动态婴儿消化模型:强化1,3-二油基-2-棕榈酰甘油、乳铁蛋白和酪蛋白磷酸肽对婴儿配方奶粉蛋白质和脂肪消化的影响
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-23 DOI: 10.1002/jsfa.70191
Sehar Anwar, Lina Zhang, Yingxiong Zhong, Xiaoyu Peng, Danying Guo, Jiaqi Wang, Wei Li, Peng Zhou
{"title":"In vitro dynamic infant digestion model: effects of 1,3-dioleoyl-2-palmitoyl glycerol, lactoferrin, and casein phosphopeptide fortification on infant formula protein and fat digestion.","authors":"Sehar Anwar, Lina Zhang, Yingxiong Zhong, Xiaoyu Peng, Danying Guo, Jiaqi Wang, Wei Li, Peng Zhou","doi":"10.1002/jsfa.70191","DOIUrl":"https://doi.org/10.1002/jsfa.70191","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the effects of fortifying infant formulas (IFs) with casein phosphopeptide (CPP), lactoferrin (LTF), and 1,3-dioleoyl-2-palmitoyl glycerol (OPO) on protein and fat digestion, using an in vitro infant dynamic digestion model with human milk (HM) as the standard. Four formulas were examined: an unfortified control (CC) and three fortified variants (F1, F2, and F3), each containing the same fortifying components at varying concentrations.</p><p><strong>Results: </strong>Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that most proteins were completely hydrolyzed during gastrointestinal digestion, except β-lactoglobulin (β-Lg). After 180 min, protein digestibility ranged from 97.6% to 99.08%, with F2 reaching the highest value (99.08%), comparable to HM (98.92%). Amino acid fit analysis indicated strong similarity to HM in F2 (96.67%) and F3 (94.87%). Protein hydrolysis and free amino acid (FAA) release were significantly greater (P < 0.05) in F2 and F3 than in CC and F1. The fortified IFs showed peptide profiles and lipolysis degrees (LDs) closer to HM than the control, although HM had the greatest peptide release. In total, 145 bioactive peptides were identified, 77 of which were common to all IFs.</p><p><strong>Conclusion: </strong>These findings suggest that fortification with CPP, LTF, and OPO can modulate the protein and fat digestion patterns in IF, increasing its similarity to HM. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A novel short-chain dehydrogenase/reductase catalyzing efficient patulin detoxification: identification and computational characterization. 一种新型短链脱氢酶/还原酶催化高效展霉素解毒:鉴定和计算表征。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-22 DOI: 10.1002/jsfa.70213
Binbin Ouyang, Wenli Zhang, Cuie Guang, Wei Xu, Junmei Ding, Wanmeng Mu
{"title":"A novel short-chain dehydrogenase/reductase catalyzing efficient patulin detoxification: identification and computational characterization.","authors":"Binbin Ouyang, Wenli Zhang, Cuie Guang, Wei Xu, Junmei Ding, Wanmeng Mu","doi":"10.1002/jsfa.70213","DOIUrl":"https://doi.org/10.1002/jsfa.70213","url":null,"abstract":"<p><strong>Background: </strong>Patulin (PAT), a widespread mycotoxin produced by Aspergillus and Penicillium genera, commonly contaminates fruits, vegetables and their derived products. Due to its water solubility, PAT poses significant health risks through food-chain accumulation.</p><p><strong>Results: </strong>In this study, a novel short-chain dehydrogenase/reductase (SDR) from Lachancea mirantina (LmSDR) is reported, which achieves complete PAT degradation under pH 6.0 and 50 °C. LmSDR retains high activity across a pH range of 5.0-6.5. In the presence of NADPH, LmSDR exhibits excellent substrate tolerance, degrading over 70% of PAT even at high concentrations (50 μg mL<sup>-1</sup>). Furthermore, LmSDR demonstrates considerable thermal stability, maintaining more than 85% residual activity after a 1 h incubation at 40 °C, with a melting temperature of 44 °C. Using AI-assisted structural modeling and molecular docking, three pivotal residues, Tyr156, Ser140 and His185, were identified as critical for NADPH-mediated proton transfer, facilitating PAT reduction to less toxic (E)-ascladiol. Moreover, in silico mutagenesis pinpointed several residues essential for LmSDR-mediated PAT degradation, thereby providing a theoretical basis for the rational engineering of LmSDR and other PAT-degrading SDRs.</p><p><strong>Conclusion: </strong>The acidic pH preference of LmSDR indicates its optimal activity under mildly acidic conditions, highlighting its potential as a biocatalyst for applications in acidic food matrices. Moreover, computer-aided approaches offer a theoretical framework for elucidating the proton transfer mechanism of LmSDR and other PAT-degrading SDRs. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accessing the impact of harvest weights of Tenebrio molitor on amino acid digestibility and metabolizable energy in cecectomized laying hens. 探讨采食量对切除盲肠蛋鸡氨基酸消化率和代谢能的影响。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-22 DOI: 10.1002/jsfa.70196
Adewunmi Omotoso, Elena Werner, Nils Hautkapp, Markus Rodehutscord, Wolfgang Siegert
{"title":"Accessing the impact of harvest weights of Tenebrio molitor on amino acid digestibility and metabolizable energy in cecectomized laying hens.","authors":"Adewunmi Omotoso, Elena Werner, Nils Hautkapp, Markus Rodehutscord, Wolfgang Siegert","doi":"10.1002/jsfa.70196","DOIUrl":"https://doi.org/10.1002/jsfa.70196","url":null,"abstract":"<p><strong>Background: </strong>The effects of harvest weights on composition, amino acid (AA) digestibility and metabolizable energy (ME<sub>N</sub>) of Tenebrio molitor (TM) were investigated using cecectomized laying hens. The partially defatted and dried TM variants comprised median individual frass-free harvest weights of 60, 80, 100, and 120 mg of larvae, and a pupae weight of 125 mg. Diets containing the five TM variants and a basal diet were fed to six cecectomized laying hens over six experimental periods in a 6 × 6 Latin square design.</p><p><strong>Results: </strong>The AA concentrations relative to crude protein were similar among the TM larvae variants. Crude protein and crude fat contents did not differ between the larvae variants. Pupae had a lower crude protein and a higher crude fat concentration compared to the larvae variants. The AA concentrations in the fat-free dry matter of TM were low for all harvest weights. Larvae variants did not differ in AA digestibility, whereas pupae showed the highest values. Compared with pupae, larvae had a lower digestibility (P ≤ 0.050) for all AA except Arg and Thr in L60; Arg, Asx, Glx, Lys, and Thr in L100, and all AA except Arg, Asx, Glx, Leu, Phe, Pro, and Thr in L120. The ME<sub>N</sub> did not differ among larvae variants but was higher for the pupae (P ≤ 0.050).</p><p><strong>Conclusion: </strong>The present study did not indicate that partially defatted TM larvae of different harvest weights influenced AA digestibility and ME<sub>N</sub>, whereas AA digestibility and ME<sub>N</sub> of pupae was higher. Feeding partially defatted TM pupae instead of larvae could reduce nitrogenous emissions during egg production, provided that emissions from pupae production remain low. Therefore, optimizing the defatting process for TM pupae may help mitigate challenges associated with the higher fat content, potentially enabling the realization of the benefits from its higher AA digestibility in feed applications. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial-mesenchymal transition, and metastasis in breast cancer. 从藤黄中纯化的Chamuangone抑制乳腺癌的炎症、血管生成、上皮-间质转化和转移。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-22 DOI: 10.1002/jsfa.70175
Asad Ur Rahman, Naveed Ullah Khan, Abdul Basit, Muhammad Khan, Jiang Ni, Pharkphoom Panichayupakaranant
{"title":"Chamuangone purified from Garcinia cowa inhibits inflammation, angiogenesis, epithelial-mesenchymal transition, and metastasis in breast cancer.","authors":"Asad Ur Rahman, Naveed Ullah Khan, Abdul Basit, Muhammad Khan, Jiang Ni, Pharkphoom Panichayupakaranant","doi":"10.1002/jsfa.70175","DOIUrl":"https://doi.org/10.1002/jsfa.70175","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BC) is a life-threatening disease, with approximately 60% of cases progressing to breast cancer lung metastasis (BCLM). Chamuangone, a key bioactive constituent derived from the leaves of the Thai plant Garcinia cowa Roxb., has demonstrated significant potential as an anticancer agent. To evaluate its anticancer effects, chamuangone was purified from the leaves and assessed through in silico, in vitro, and in vivo studies using a mouse model of BC.</p><p><strong>Results: </strong>The results revealed that chamuangone (10 mg L<sup>-1</sup>) inhibited cancer cell proliferation by inducing G0/G1 phase arrest and apoptosis in 4T1-luciferase (4T1-luc) and Michigan Cancer Foundation-7 (MCF-7) cells. It also modulated the levels of key apoptotic markers, including B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and caspase-3. Similarly, in vivo studies demonstrated that chamuangone significantly (P < 0.05) inhibited breast tumor growth and lung metastasis at a dose of 100 mg kg⁻¹ body weight, in comparison with the standard drug doxorubicin (5 mg kg⁻¹ body weight) in mice. Immunohistochemical analysis further revealed that chamuangone modulated several BCLM-associated markers, including estrogen receptor (ER), progesterone receptor (PR), Ki-67 antigen (Ki-67), cluster of differentiation 44 (CD44), E-cadherin, N-cadherin, vascular endothelial growth factor A (VEGFA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in both breast tumors and lung tissues. These effects contributed to the inhibition of inflammation, angiogenesis, epithelial-mesenchymal transition (EMT), cancer cell proliferation, and metastasis. In silico studies also demonstrated strong interactions between chamuangone and BCLM-related targets, along with encouraging pharmacokinetic and toxicokinetic profiles.</p><p><strong>Conclusion: </strong>This investigation highlights the potential uses of chamuangone as a chemopreventive agent and functional component for managing the advanced stages of BC. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The physical and chemical properties of complexes of geniposide and heat-treated soybean protein isolate and effect on the bioavailability of geniposide. 京尼平苷与热处理大豆分离蛋白配合物的理化性质及其对京尼平苷生物利用度的影响。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-21 DOI: 10.1002/jsfa.70205
Suqin Lin, Fuqiang Zhao, Silin Feng, Haowei Fan, Xinchao He, Wenjing Ma, Guiming Fu, Yin Wan
{"title":"The physical and chemical properties of complexes of geniposide and heat-treated soybean protein isolate and effect on the bioavailability of geniposide.","authors":"Suqin Lin, Fuqiang Zhao, Silin Feng, Haowei Fan, Xinchao He, Wenjing Ma, Guiming Fu, Yin Wan","doi":"10.1002/jsfa.70205","DOIUrl":"https://doi.org/10.1002/jsfa.70205","url":null,"abstract":"<p><strong>Background: </strong>Geniposide (GE) exhibits diverse biological activities, but its poor stability and low bioavailability limit its applications. Combining GE with proteins can improve its stability. Heat-treated soybean protein isolate (HSPI) is widely used to transport bioactive substances due to its loading capacity. This study investigated the structural and functional changes in HSPI induced by its interaction with GE, as well as the resulting effects on GE's stability and bioavailability.</p><p><strong>Results: </strong>Results showed that GE bonded to CN, CO and NH groups in HSPI via hydrogen bonding, altering the protein's secondary structure. The resulting GE-HSPI complexes exhibited superior foaming capacity, thermal stability, storage stability and ionic stability compared to free GE. In pharmacokinetic studies, animals fed with GE-HSPI complexes exhibited higher plasma GE concentration than those given GE solution alone. Ultimately, the oral bioavailability of GE in GE-HSPI complexes was around twofold higher than that of the GE solution, with GE and 80 °C-treated SPI complexes achieving the highest bioavailability (7.90%).</p><p><strong>Conclusion: </strong>These findings highlight the potential use of GE-HSPI complexes as an effective strategy to enhance GE bioavailability. They also pave the way for further research into gardenia-derived applications in functional foods or pharmaceuticals. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing functional properties of fruit juices with highly active xylanase from Aspergillus tubingensis FAT35. 利用高活性塔宾曲霉FAT35木聚糖酶提高果汁的功能特性。
IF 3.5 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-09-21 DOI: 10.1002/jsfa.70208
Marina Ristović, Sanja Stojanović, Marija Pavlović, Aleksandra Margetić, Marinela Šokarda Slavić, Biljana Dojnov, Zoran Vujčić
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