Enhancing β-carotene bioavailability and protective effects for dextran sodium sulfate-induced colitis with sesame protein hydrolysate nanoparticles.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-06-12 DOI:10.1002/jsfa.14438

Dounya Zad Oumeddour, Xin Ai, Yaqi Liu, Yutong Yuan, Zehui Zhu, Mingxin Zhang, Lei Zhao, Liang Zhao

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引用次数: 0

Abstract

Background: Inflammatory bowel disease (IBD) is a chronic condition marked by recurring intestinal inflammation, leading to symptoms such as fatigue, diarrhea, and weight loss. Current treatments are limited, highlighting the need for new therapeutic strategies. β-Carotene (BC), a natural antioxidant, has potential in managing colitis, but its low bioavailability limits its efficacy. This study evaluated a novel BC-loaded sesame protein hydrolysate nanoparticles (BC-SPH NPs) formulation to enhance BC bioavailability and therapeutic performance in a dextran sodium sulfate (DSS)-induced colitis model.

Results: BC-SPH NPs showed an average size of 50.30 ± 0.43 nm, an encapsulation efficiency of 75.23 ± 0.92%, a prolonged plasma residence profile, with a time to reach maximum plasma concentration (T_max) of 8 h, and a peak plasma concentration of 64.982 ng mL^-1. Notably, the NPs enabled enhanced tissue distribution of BC to key tissues in rats including kidneys, liver, small intestine, and colon. In DSS-induced colitis mice, BC-SPH NPs significantly reduced disease activity index, improved body weight, preserved colon length and structure, and prevented inflammatory cell infiltration. Additionally, BC-SPH NPs lowered serum interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor alpha (TNF-α), as well as colonic myeloperoxidase (MPO) activity and nitric oxide (NO) levels. Furthermore, BC-SPH NPs up-regulated tight junction proteins Claudin-1, ZO-1, and Occludin, indicating improved intestinal barrier integrity. In contrast, free BC provided only modest effects.

Conclusion: This study demonstrates that BC-SPH NPs substantially enhance the bioavailability and therapeutic effects of BC in DSS-induced colitis compared to free BC. The nanoformulation effectively alleviates colitis symptoms, preserves intestinal integrity, and reduces inflammation, suggesting that BC-SPH NPs represent a promising natural therapeutic option for the prevention and treatment of ulcerative colitis. © 2025 Society of Chemical Industry.

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芝麻蛋白水解纳米颗粒提高β-胡萝卜素的生物利用度和对葡聚糖硫酸钠诱导结肠炎的保护作用。

背景：炎症性肠病（IBD）是一种以反复出现的肠道炎症为特征的慢性疾病，可导致疲劳、腹泻和体重减轻等症状。目前的治疗是有限的，强调需要新的治疗策略。β-胡萝卜素（BC）是一种天然抗氧化剂，具有治疗结肠炎的潜力，但其低生物利用度限制了其疗效。本研究评估了一种新型负载BC的芝麻蛋白水解纳米颗粒（BC- sph NPs）配方，以提高BC在葡聚糖硫酸钠（DSS）诱导的结肠炎模型中的生物利用度和治疗效果。结果：BC-SPH NPs平均粒径为50.30±0.43 nm，包封效率为75.23±0.92%，血浆停留时间较长，达到最大血药浓度（Tmax）时间为8 h，血药浓度峰值为64.982 ng mL-1。值得注意的是，NPs增强了BC在大鼠肾、肝、小肠和结肠等关键组织中的组织分布。在dss诱导的结肠炎小鼠中，BC-SPH NPs显著降低疾病活动指数，改善体重，保留结肠长度和结构，并阻止炎症细胞浸润。此外，BC-SPH NPs降低血清白细胞介素-6 （IL-6）、白细胞介素-1β (IL-1β)和肿瘤坏死因子α (TNF-α)，以及结肠髓过氧化物酶（MPO）活性和一氧化氮（NO）水平。此外，BC-SPH NPs上调紧密连接蛋白Claudin-1、ZO-1和Occludin，表明肠道屏障完整性得到改善。相比之下，游离BC只提供了适度的效果。结论：本研究表明，与游离BC相比，BC- sph NPs显著提高了BC在dss诱导结肠炎中的生物利用度和治疗效果。该纳米制剂可有效缓解结肠炎症状，保持肠道完整性，减少炎症，表明BC-SPH NPs是预防和治疗溃疡性结肠炎的一种有前景的天然治疗选择。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.