孢子展示解锁葡萄糖氧化酶新底物的创新技术。

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-06-13 DOI:10.1002/jsfa.14420

Xihua Chen, Zeyang Li, Zhengfen Wu, Yong Feng, Yong Chen, Yi Ma, Zhongjian Guo, Huayou Chen

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引用次数: 0

摘要

背景：葡萄糖氧化酶（GOD）在动物饲料工业中有着广泛的应用。然而，它的底物特异性限制了它在纤维原料的广泛降解中的应用。结果：本研究利用一系列孢子展示技术，将GOD从β-d-葡萄糖特异性酶转化为能够催化阿拉伯糖、木糖、蔗糖、纤维素二糖、棉子糖和山梨醇等多种糖的广谱糖氧化酶。阿拉伯糖、木糖和山梨糖醇的相对酶活性分别从不足5%提高到71%、52%和53%。此外，GOD活性的最佳温度从30°C变为60°C，显著提高了其热稳定性。结论：高活性、耐热性强、能催化多种糖类的酶有助于减少饲料中水解产物的积累，提高饲料的成本效益。这有助于推进可持续农业和饲料生产的进一步发展。©2025化学工业协会。

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Spore display innovation technology for unlocking new substrates for glucose oxidase.

Background: Glucose oxidase (GOD) has been extensively applied in the animal feed industry. However, its substrate specificity limits its utility in the broad degradation of fibrous raw materials.

Results: In this study, a series of spore display technologies were employed to transform GOD from an enzyme specific for β-d-glucose into a broad-spectrum sugar oxidase capable of catalyzing various sugars such as arabinose, xylose, sucrose, cellobiose, raffinose, and sorbitol. Notably, the relative enzyme activities for arabinose, xylose, and sorbitol increased from less than 5% to 71%, 52%, and 53%, respectively. Furthermore, the optimal temperature for GOD activity shifted from 30 °C to 60 °C, significantly improving its thermal stability.

Conclusion: Enzymes with high activity, strong heat resistance, and the ability to catalyze various sugars can help reduce the accumulation of hydrolysis products in animal feed, improving the cost-effectiveness of the feed. This contributes to the advancement of sustainable agriculture and the further development of feed production. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.