Journal of Zoo and Wildlife Medicine最新文献

{"title":"AUTOLOGOUS OR ALLOGENIC BLOOD PLEURODESIS AS TREATMENT FOR PNEUMOCOELOM IN FOUR SEA TURTLES (<i>LEPIDOCHELYS KEMPII</i>, <i>CARETTA CARETTA</i>).","authors":"Melanie Dittmer, Heather Barron, Laura Kellow, Shane M Boylan, Sarah E Hirsch, Justin R Perrault, Bette Zirkelbach, Terry M Norton","doi":"10.1638/2023-0097","DOIUrl":"10.1638/2023-0097","url":null,"abstract":"<p><p>Pneumocoelom secondary to primary pulmonary disease was diagnosed via CT (three cases) or radiographs and coelioscopy (one case) in one Kemp's ridley (<i>Lepidochelys kempii</i>) and three loggerhead (<i>Caretta caretta</i>) sea turtles. All cases were suspected to be caused by trauma to the lung, and all exhibited positive buoyancy disorders. Coelomocentesis alone was ineffective at resolving each pneumocoelom, suggesting a large and persistent tear in the pulmonary parenchyma. An autologous (in three cases) or allogenic (in one case) blood patch pleurodesis was performed. The pneumocoelom was resolved in all four cases with no noted postoperative complications. Follow-up diagnostic imaging confirmed resolution of the pneumocoelom in three cases, and all four patients were successfully rehabilitated and had normal buoyancy at the time of release. One rehabilitated loggerhead was satellite tagged, and the tracking data demonstrated that the animal was still alive and moving normally 126 d postrelease, when the tag stopped transmitting. In sea turtles, an autologous or allogenic blood patch pleurodesis can be safely performed in cases of persistent pneumocoelom secondary to traumatic primary pulmonary disease.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UNILATERAL OVARIAN TORSION IN FIVE GECKOS: CLINICAL AND ULTRASOUND FINDINGS.","authors":"Clément Paillusseau, Frédéric Gandar, Camille Francois, Lionel Schilliger","doi":"10.1638/2024-0022","DOIUrl":"10.1638/2024-0022","url":null,"abstract":"<p><p>Few cases of antemortem ovarian torsion and diagnosis have been described in reptiles. This case series reports clinical and ultrasound findings in five adult (aged 1-6 yr) female geckos (three leopard geckos [<i>Eublepharis macularius</i>], one crested gecko [<i>Correlophus ciliatus</i>], and one gargoyle gecko [<i>Rhacodactylus auriculatus</i>]) diagnosed with unilateral ovarian torsion between 2019 and 2023. All animals presented with acute weakness associated with coelomic distension, and one suffered from chronic diarrhea and cachexia. Coelomic ultrasound examination (12-MHz linear probe) revealed signs of bilateral follicular stasis and oophoritis in all cases (heterogenic follicles ≤1.3 cm diameter) associated with a large anechoic periovarian rim and a hyperechoic chord, consistent with twisted ovarian vessels. Blood supply to ovarian structures was not detected using a Doppler flow, and a unilateral ovarian torsion was diagnosed in all geckos. A bilateral ovariectomy was performed under general anesthesia in all five animals. The mass of the excised ovaries varied between 7 to 15 g (12.7-22.2% of body weight). One gecko died 1 d postsurgery; the four remaining animals were healthy 6 mon postsurgery. Gekkonids are unique among reptiles in that they undergo a monoautochronic ovulation (only one follicle is recruited by each ovary during each ovarian cycle); the presence of multiple vitellogenic follicles on each ovary facilitates the diagnosis of follicular stasis. This condition was present in all five geckos and was suspected to have led to ovarian torsion. This case series emphasizes the value of ultrasound examination for antemortem diagnosis of reproductive disorders in reptiles.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMANGIOSARCOMA IN RED WOLVES (<i>CANIS RUFUS</i>) AND GRAY WOLVES (<i>CANIS LUPUS</i>) IN HUMAN CARE: SIX CASES.","authors":"Jasmine Y Sarvi, Katherine E Dowling, Julie Swenson, Holly J Haefele, Michael M Garner","doi":"10.1638/2024-0007","DOIUrl":"10.1638/2024-0007","url":null,"abstract":"<p><p>Wolves are commonly housed in zoological institutions and captive breeding facilities that are essential for maintaining genetic diversity and for the recovery of declining populations. Neoplasia is a common cause of mortality in wolves, but hemangiosarcoma has not previously been described. This condition was diagnosed in four red wolves (<i>Canis rufus</i>) and two gray wolves (<i>Canis lupus</i>) housed at five different institutions between 2008 and 2018. Animals were 11-16 yr of age at the time of presentation. Clinical signs included loss of body condition, abdominal distension, lethargy, weakness, ataxia, and hyporexia. Three animals were mildly anemic. All animals were humanely euthanized within an average of 3 d from onset of clinical signs. Two animals had primary splenic tumors, two had pelvic tumors with one originating from the aorta, and one had a cranial mediastinal mass. Diagnosis was made on postmortem histologic examination in all cases. Four wolves had evidence of metastases with foci in the lungs, lymph nodes, mesentery, liver, subcutis/skeletal muscle, kidney, adrenal, and thyroid gland. Hemangiosarcoma should be considered in geriatric wolves presenting with nonspecific signs, particularly if abdominal distension, free peritoneal fluid, or anemia is present.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INDUCTION OF PARTURITION IN A PYGMY HIPPOPOTAMUS (<i>CHOEROPSIS LIBERIENSIS</i>).","authors":"Brianne E Phillips, Carlos Gradil, Eric Baitchman","doi":"10.1638/2023-0106","DOIUrl":"10.1638/2023-0106","url":null,"abstract":"<p><p>A 27-yr-old female pygmy hippopotamus (<i>Choeropsis liberiensis</i>) had two consecutive stillbirths with no overt signs of labor, suggestive of uterine inertia. After a third pregnancy was confirmed, an induction protocol was developed. Cloprostenol and betamethasone were administered on d 200 of gestation (time 0 h). Additional doses of cloprostenol were administered at 24 and 48 h and oxytocin at 30, 31, and 48 h. Each injection resulted in preparturient behavior without overt evidence of contractions. Fetal membranes presented at the vulva at 54.5 h after initial cloprostenol and betamethasone administration with no progression of labor. Transvaginal palpation and manual delivery of a live calf followed. Despite confirmed nursing, the serum glutaraldehyde coagulation test was negative. Failure of passive transfer may have been secondary to the induction protocol. The calf was treated with broad-spectrum antimicrobial agents due to diarrhea, and clinical signs resolved. This clinical brief details the first known induction of parturition in a pygmy hippopotamus, which can serve as the basis for further development of the technique.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEALTH ASSESSMENT OF SPOTTED (<i>CLEMMYS GUTTATA</i>) AND PAINTED (<i>CHRYSEMYS PICTA</i>) TURTLES IN CAPE COD, MASSACHUSETTS, U.S.A, WITH DETECTION OF A NOVEL ADENOVIRUS.","authors":"Lauren M Vincent, Matthew C Allender, Annie E Curtis, John C Garrison, Stacey Lance, Adam McFall, Amber Simmons, Kaitlin Moorhead, Laura Adamovicz","doi":"10.1638/2023-0141","DOIUrl":"https://doi.org/10.1638/2023-0141","url":null,"abstract":"<p><p>Freshwater turtles face numerous anthropogenic threats worldwide. Health assessments are a key component of chelonian population assessment and monitoring but are under reported in many species. The purpose of this study was to characterize the health of spotted turtles (<i>Clemmys guttata</i>; n = 30) and painted turtles (<i>Chrysemys picta</i>; n = 24) at Camp Edwards, a military base in Cape Cod, Massachusetts, using physical examinations, hematology, plasma heavy metal analyses, and pathogen surveillance via PCR. Spotted turtles had a high prevalence of carapace (n = 27, 90%) and plastron (n = 14, 46.7%) lesions, and a previously undescribed adenovirus was detected in three animals (proposed as <i>Clemmys</i> adenovirus-1). Female painted turtles had lower plasma copper (p = 0.012) and higher strontium (p = 0.0003) than males, and appeared to be in a similar plane of health to previous reports. This initial health assessment effort provides useful baseline data for future comparison in these species. Conservation efforts on Camp Edwards should incorporate continued health surveillance of these populations to identify intervention opportunities and determine the conservation threats, if any, of the novel adenovirus.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMATOLOGY AND BIOCHEMICAL REFERENCE INTERVALS FOR FREE-RANGING PRONGHORN (<i>ANTILOCAPRA AMERICANA</i>) IN WEST TEXAS.","authors":"Ashley M Reeves, Shawn S Gray, Louis A Harveson, Clayton D Hilton, Cary M Springer, Warren C Conway, Robert O Dittmar","doi":"10.1638/2023-0119","DOIUrl":"https://doi.org/10.1638/2023-0119","url":null,"abstract":"<p><p>Pronghorn (<i>Antilocapra americana</i>) are considered a keystone species of North American grasslands and an important economic source for many landowners in Texas. Pronghorn restoration projects routinely capture and translocate individuals from surplus populations to restoration areas. The objective of this study was to generate normal hematological and biochemical reference intervals (RI) for free-ranging pronghorn populations in Texas as a health monitoring tool for pronghorn restoration efforts. Blood samples were collected by jugular venipuncture and divided among an EDTA tube, serum separator tube, and a single blood smear on site. Complete blood counts and biochemical profiles were completed at the Texas Veterinary Medical Diagnostic Laboratory. In total, 417 individuals (41 males, 376 females) were included in the analysis. RI were determined by robust methods (R Studio) and mixed models' analysis of variance (SPSS 28) to examine differences in blood parameters due to fever, sex, age (adult versus yearling [<1 yr of age]), cell abnormalities, and pathogen exposure reported by the testing laboratory. Sex, age, and pathogen exposure affected mean blood values, but did not warrant development of separate RI by class. Bluetongue virus was identified in 46.8% (195/417) of pronghorns and epizootic hemorrhagic disease in 89.4% (194/217) of pronghorns; 84.8% (184/217) of the pronghorns tested positive for both diseases. This information provides baseline hematology and biochemical parameters to assess the health of free-ranging pronghorn and guide wildlife managers in decision-making for future translocations and restoration objectives.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PROTEIN ELECTROPHORESIS AND REFERENCE INTERVALS OF SERUM PROTEINS OF A CAPTIVE POPULATION OF ROTI ISLAND SNAKE-NECKED TURTLES (<i>CHELODINA MCCORDI</i>).","authors":"Shin Min Chong, Guillaume Douay","doi":"10.1638/2023-0104","DOIUrl":"https://doi.org/10.1638/2023-0104","url":null,"abstract":"<p><p>Protein electrophoresis (PEP) is an important tool in mammals to characterize specific dysproteinemias and detect acute and chronic inflammatory responses. In reptiles, PEP is the gold standard method for globulin fraction determination and albumin measurement. In this study, preliminary reference intervals were established for serum PEP in 22 clinically healthy adult Roti Island snake-necked turtles (<i>Chelodina mccordi</i>), a critically endangered species, kept in captivity and sampled over two monsoon seasons. The species has a prominent prealbumin fraction and β-globulins were the dominant globulin fraction. Significant differences between females and males were found in prealbumin (<i>P</i> < 0.01), albumin (<i>P</i> = 0.02), α1-globulin (<i>P</i> = 0.05) and γ-globulin (<i>P</i> = 0.01). Gravid females had significantly lower total protein (<i>P</i> < 0.01), prealbumin (<i>P</i> < 0.01), albumin (<i>P</i> < 0.01) and albumin:globulin ratio (<i>P</i> = 0.01). These preliminary reference intervals should aid in clinical investigation in this species as well as further research studies seeking to understand the application of PEP in reptilian species.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AEROBIC BLOOD CULTURES AND COMPARISON TO CLINICAL FINDINGS OF FREE-RANGING GREEN TURTLES (<i>CHELONIA MYDAS</i>) IN EAST CENTRAL FLORIDA.","authors":"Alan R Glassman, Trevor T Zachariah, Jessica L Patterson, Katherine L Mansfield, Erin E Seney, Akhilesh Ramachandran","doi":"10.1638/2023-0107","DOIUrl":"https://doi.org/10.1638/2023-0107","url":null,"abstract":"<p><p>Positive blood cultures have been identified in debilitated, stranded, and deceased green turtles (<i>Chelonia mydas</i>), suggestive of septicemia. Interpretation of these results is often difficult because multiple studies have previously identified bacteremia in clinically healthy reptiles. In this study, paired blood cultures and skin cultures obtained after aseptic preparation of the venipuncture site were collected from 50 immature free-ranging green turtles from Port Canaveral, Florida. Blood culture results were compared with health status (apparently healthy versus unhealthy, based on physical examination findings and appropriate body condition), date of collection, presence of external fibropapillomatosis, healed or unhealed injuries, and presence of barnacles. Weight, body condition score, body condition index, morphometric measures, volume of blood collected, and body temperature were compared between blood culture-positive and blood culture-negative turtles. Positive blood cultures were identified in 14% (7 of 50) of all turtles, including 15.6% (5 of 32) of apparently healthy turtles. <i>Vibrio</i> spp., <i>Bacillus megaterium</i>, <i>Cellulomonas</i> sp., and <i>Staphylococcus pasteuri</i> were isolated in blood culture from apparently healthy individuals. There was a significant association (<i>P</i> = 0.048) between positive skin cultures and positive blood cultures, but isolates obtained were consistently different between paired results. There was no significant association (<i>P</i> > 0.05) between blood culture results and health status, evidence of healed or unhealed injuries, external fibropapillomatosis, or presence of barnacles. Based on the results of this study, positive blood cultures suggestive of nonclinical bacteremia may be present in apparently healthy green turtles. The results of this study will aid the attending clinician in interpretation of blood culture results of apparently healthy or presumed septicemic captive and rehabilitating green turtles as part of the conservation and population recovery of this threatened species.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERIAL CHANGES IN METABOLIC ACID-BASE STATUS IN THREE SPECIES OF ANESTHETIZED CAPTIVE LARGE FELID.","authors":"Nigel Dougherty, Brett Gartrell, Vicki Walsh, James Chatterton, Baukje Lenting","doi":"10.1638/2022-0072","DOIUrl":"https://doi.org/10.1638/2022-0072","url":null,"abstract":"<p><p>Determination of acid-base status contributes important information about patient health, including for patients under anesthesia. There is a paucity of information about the determinants of acid-base status of large felids managed under anesthesia, and advancement of such knowledge may contribute to patient safety. This study serially monitored the individual metabolic acid-base status of 11 large felids, including lions (<i>Panthera leo</i>), tigers (<i>Panthera tigris</i>), and cheetahs (<i>Acinonyx jubatus</i>), under general anesthesia. We analyzed the contributions of measured strong ions (sodium, chloride, potassium, lactate), weak acids and buffers (albumin, phosphate and bicarbonate), and unmeasured anions to standardized extracellular base excess (SBE). A general linear model assessed for species differences in these parameters, with time since immobilization, SBE, and mean arterial pressure as covariates. By employing a Stewart-based analytical approach, it was possible to separate chloremic and unmeasured anion contributions to metabolic acid base status. This provided a basis for identifying mixed metabolic processes, generating differentials for underlying causes. Using normal acid base parameters for domestic felids, metabolic acidosis was found to be prevalent. Frequent evidence of unmeasured anion accumulation was also found, with unmeasured anions occasionally exceeding 5mmol/L. These findings warrant further inquiry into the drivers and clinical significance of metabolic acidosis and unmeasured anion accumulations in anesthetized large felids, encouraging further anion identity studies to elucidate possible causes. Reference ranges need to be established for acid-base parameters in large felids as a foundation for interpreting more controlled, prospective research into determinants of metabolic acid-base status in these animals under anesthesia.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEMATOLOGIC AND PLASMA BIOCHEMICAL REFERENCE VALUES FOR CAPTIVE LEOPARD SHARKS (<i>TRIAKIS SEMIFASCIATA</i>).","authors":"Amanda J Arndt, Meghan M Holst, Jill V Spangenberg","doi":"10.1638/2022-0097","DOIUrl":"https://doi.org/10.1638/2022-0097","url":null,"abstract":"<p><p>Leopard sharks (<i>Triakis semifasciata</i>) are temperate, Eastern Pacific elasmobranchs popular in public aquariums. Blood analysis is commonly used for assessing animal health, yet reference values have not been established for this species. This study analyzed <i>T. semifasciata</i> population data to characterize blood reference values for a collection of <i>T. semifasciata</i> housed at a public aquarium. Twenty-one captive leopard sharks were sampled. Blood was collected during annual health examinations from sedated animals. After collection, blood samples were anticoagulated with lithium heparin, and hematocrit and plasma biochemistry values were analyzed. The minimum-maximum ranges were hematocrit 11-31%, buffy coat 1-2%, glucose 4.94-9.38 mM/L, sodium 244-272 mM/L, potassium 3.7-5.5 mM/L, chloride 214-246 mM/L, aspartate aminotransferase 5-31 U/L, creatine kinase 36-1,136 U/L, calcium 3.65-3.95 mM/L, phosphorus 1.13-2.23 mM/L, total protein 21-38 g/L, and total CO₂ 12-18 mM/L. The values identified will contribute to a better understanding of captive leopard shark physiology and to improved veterinary care for captive leopard sharks. Further research can examine the validity of machines like the Vetscan VS2, which will expand the resources available to care professionals.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}