Journal of Wildlife Diseases最新文献_第4页

Dogs can Generalize from Cotton Training Aids to Fecal Matter in Chronic Wasting Disease Detection. 犬在慢性消耗性疾病的检测中可以从棉花训练辅助物到粪便进行推广。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00093

Amritha Mallikarjun, Clara Wilson, Ila Charendoff, Madison B Moore, Elizabeth Nguyen, Abigail J Hendrzak, Michelle Gibison, Cynthia M Otto

{"title":"Dogs can Generalize from Cotton Training Aids to Fecal Matter in Chronic Wasting Disease Detection.","authors":"Amritha Mallikarjun, Clara Wilson, Ila Charendoff, Madison B Moore, Elizabeth Nguyen, Abigail J Hendrzak, Michelle Gibison, Cynthia M Otto","doi":"10.7589/JWD-D-24-00093","DOIUrl":"10.7589/JWD-D-24-00093","url":null,"abstract":"Chronic wasting disease (CWD) is a prion-associated transmissible spongiform encephalopathy (TSE) that causes fatal neurodegeneration in cervids. One promising method for CWD surveillance is the use of detection dogs trained to differentiate between fecal matter from CWD-positive (infected) and CWD-negative (noninfected) deer. However, using actual fecal matter during the training process confers a biohazard risk. Chronic wasting disease detection dogs trained on fecal matter can differentiate between CWD-positive and CWD-negative training aids, including cotton aids, which are inexpensive and simple to use. However, as most dogs in an applied setting would instead be trained on aids and tasked with finding actual fecal matter in the field, this study examined 1) the extent to which dogs initially trained on CWD-incubated cotton can discriminate between fecal samples from CWD-positive and CWD-negative deer; and 2) the impact of substrate form (cotton ball or cotton roll) on the dogs' detection accuracy. Results show that dogs (n=5) maintained an accuracy of >80% during training while discriminating between cotton ball training aids that had been incubated (noncontact) with deer fecal samples confirmed as either CWD-positive or CWD-negative. When tested with cotton ball training aids incubated with novel samples, dogs (n=4) showed 73% sensitivity and 77% specificity. When presented with deer fecal samples, dogs showed a sensitivity of 50% and a specificity of 90%. The type of cotton (ball or roll) did not significantly influence performance. In sum, cotton aids can be used to train dogs to detect the CWD odor profile effectively. However, an initial reduction in sensitivity suggests that integrating exposure to target fecal matter may enhance detection performance.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"600-608"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Prevalence of Antigens of and Specific Antibodies Against Various Viral Pathogens in European Wildcats (Felis silvestris) from Southwest Germany, 2020-22. 2020-2022年德国西南部欧洲野猫（Felis silvestris）各种病毒病原体抗原和特异性抗体的高流行率

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00191

Sarah Pauline Stubbe, Johannes Lang, Nicole Nagler, Simon Franz Müller, Michael Lierz

{"title":"High Prevalence of Antigens of and Specific Antibodies Against Various Viral Pathogens in European Wildcats (Felis silvestris) from Southwest Germany, 2020-22.","authors":"Sarah Pauline Stubbe, Johannes Lang, Nicole Nagler, Simon Franz Müller, Michael Lierz","doi":"10.7589/JWD-D-24-00191","DOIUrl":"10.7589/JWD-D-24-00191","url":null,"abstract":"The recent expansion of the European wildcat (Felis silvestris silvestris) and increasing numbers of domestic cats in Germany are leading to more interactions between domestic cats and wildcats. Not only hybridization, but also the reciprocal transmission of pathogens may occur. This could threaten wildcat populations, or they may act as a reservoir for their domestic relatives. In this study, 102 effusion fluid samples collected from wildcats found dead between 2020 and 2022 in the German federal state of Rhineland-Palatinate were serologically tested for antigens of and specific antibodies against viral pathogens typically found in domestic cats (feline herpesvirus [FHV], feline calicivirus [FCV], feline parvovirus [FPV], feline immunodeficiency virus [FIV], feline coronavirus [FCoV], and feline leukemia virus [FeLV]). Antigens of and/or specific antibodies against at least one virus were detected in 59% of the wildcats. Detected prevalences of specific antibodies against FHV (22.5%), FCV (20.6%), FPV (13.7%), FIV (0%), and FCoV (17.6%) were each 0-22.5%. The prevalence of antigens of FeLV was 28.4%. The high prevalence detected for FeLV antigens possibly shows that wildcats form a reservoir for this virus, as the prevalence in domestic cat populations is significantly lower.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"663-673"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shell Lesion Prevalence and Bacteriome Associations in Threatened Western Pond Turtles (Actinemys marmorata and Actinemys pallida) in California, USA. 美国加利福尼亚受威胁的西部塘龟（Actinemys marmorata和Actinemys pallida）壳损伤患病率和细菌组关联。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD D-24-00142

Nathan Green, Aria Norwood, Cyrillus Sidhe, Adrian Mutlow, Jessica Aymen, Rochelle Stiles, Jessie Bushell, Tammy Lim, Edward Culver, Natalie Reeder, Matthew Timmer, Farley Connelly, Jackie Charbonneau, Will McCall, Leslie Koenig, Madison Stein, Nicholas Geist, Max R Lambert, Obed Hernández-Gómez

{"title":"Shell Lesion Prevalence and Bacteriome Associations in Threatened Western Pond Turtles (Actinemys marmorata and Actinemys pallida) in California, USA.","authors":"Nathan Green, Aria Norwood, Cyrillus Sidhe, Adrian Mutlow, Jessica Aymen, Rochelle Stiles, Jessie Bushell, Tammy Lim, Edward Culver, Natalie Reeder, Matthew Timmer, Farley Connelly, Jackie Charbonneau, Will McCall, Leslie Koenig, Madison Stein, Nicholas Geist, Max R Lambert, Obed Hernández-Gómez","doi":"10.7589/JWD D-24-00142","DOIUrl":"10.7589/JWD D-24-00142","url":null,"abstract":"Bacteriome characterization studies can provide insights into the microbial ecology associated with disease. We collected western pond turtles (Actinemys marmorata and Actinemys pallida) from six San Francisco Bay Area, California, USA, ponds; assessed their shells for lesions; and collected shell swabs and keratin scrape samples to evaluate bacteriome differences between the whole shell (swabs) and the affected tissues (scrapes). We quantified shell lesion type and prevalence by using visual inspections of photographs collected of the plastron and carapace and then applied 16S rRNA amplicon sequencing to characterize the associated bacteriomes of shells that observed pits, ulcerations, or no lesions. We observed shell lesions at high frequencies throughout our sites, with larger individuals (>100-mm plastron length) more likely to possess injuries. We saw no differences in alpha diversities between shells presenting with lesions and those on which we did not observe lesions; however, swab samples showed higher bacterial richness than keratin scrapes. The bacterial composition within the scrapes was influenced by pond location and then lesion presence. We observed a higher relative abundance of Actinobacteriota, Bacteroidota, Cyanobacteria, and Deinococcota in the shell keratin microflora of turtles with shell lesions. Because western pond turtles are under consideration for listing under the Endangered Species Act of 1973 in the USA, understanding patterns of shell disease pathologies and the bacteria associated with disease is imperative for the management of current populations.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"574-586"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moderate Neospora caninum Detection in Wild Dog Populations in Southeast Australia. 澳大利亚东南部野狗种群中中度犬新孢子虫的检测。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00149

Eliza K Stott, Jose L Huaman, Teresa G Carvalho, Christina McCowan, Chloe J Fingland, Jade F Hammer, Alana Websdale, Lee F Skerratt, Carlo Pacioni

{"title":"Moderate Neospora caninum Detection in Wild Dog Populations in Southeast Australia.","authors":"Eliza K Stott, Jose L Huaman, Teresa G Carvalho, Christina McCowan, Chloe J Fingland, Jade F Hammer, Alana Websdale, Lee F Skerratt, Carlo Pacioni","doi":"10.7589/JWD-D-24-00149","DOIUrl":"10.7589/JWD-D-24-00149","url":null,"abstract":"Abstract: Neospora caninum is an obligate intracellular protozoan parasite that causes hind-limb paralysis in dogs and abortions in cattle. Recurrent storms of abortions in cattle in Australia are estimated to cause an economic loss of AU$100 million each year, but the source of infection in cattle (i.e., via wild or domestic definitive hosts) remains to be established. We aimed to determine potential sources of horizontal transmission of N. caninum after recent evidence that wild dogs (Canis familiaris) shed N. caninum oocysts in Victoria, Australia. To determine infection levels among wild dogs, samples were collected from three sites in Victoria (n=52) in areas of high wild dog population density (Mansfield, Swifts Creek, and Tallangatta), and N. caninum detection was determined by PCR analysis of blood and duodenal samples. Based on the PCR assay of combined duodenal samples (flotation of duodenal content and just duodenal content), this study detected N. caninum in the fecal material of 11% of wild dogs in Mansfield and 21% of wild dogs in Swifts Creek. This study provides further insight into the possible role of wild dogs as a reservoir of N. caninum in Victoria.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"714-718"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pansteatitis in Wild American Alligators (Alligator mississippiensis). 野生美洲短吻鳄（密西西比短吻鳄）的泛咽喉炎。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00190

Miriam N Boucher, Justin M Stilwell, Marisa Tellez, Shane M Boylan, Thomas R Rainwater, Stefanie L Whitmire, James T Anderson

引用次数: 0

The Efficacy of Surrogate Virus Neutralization Test ELISA Varies in the Detection of SARS-CoV-2 Antibodies with Different Postmortem White-Tailed Deer (Odocoileus virginianus) Biological Samples. 替代病毒中和试验ELISA检测不同白尾鹿死后生物标本SARS-CoV-2抗体的效果差异

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00155

Raquel Francisco, Sonia M Hernandez, Ethan P Barton, Melanie R Kunkel, Kayla G Adcock, Daniel G Mead, Mark G Ruder, Jillian R Broadhurst, Michael J Yabsley

{"title":"The Efficacy of Surrogate Virus Neutralization Test ELISA Varies in the Detection of SARS-CoV-2 Antibodies with Different Postmortem White-Tailed Deer (Odocoileus virginianus) Biological Samples.","authors":"Raquel Francisco, Sonia M Hernandez, Ethan P Barton, Melanie R Kunkel, Kayla G Adcock, Daniel G Mead, Mark G Ruder, Jillian R Broadhurst, Michael J Yabsley","doi":"10.7589/JWD-D-24-00155","DOIUrl":"10.7589/JWD-D-24-00155","url":null,"abstract":"The susceptibility of white-tailed deer (WTD; Odocoileus virginianus) to SARS-CoV-2 has resulted in WTD being one of the most studied wildlife species during the COVID-19 pandemic. However, WTD specimen collections for SARS-CoV-2 research and surveillance have largely been opportunistic. Our objective was to evaluate the detection of SARS-CoV-2 neutralizing antibodies (NAbs) in WTD serum, plus two additional WTD biological sample types, using a blocking ELISA previously used for WTD serum. The two additional sample types evaluated were eluted Nobuto filter-paper strips (FPSs) from heparinized whole blood collected perimortem from 34 WTD and serosanguinous fluid collected postmortem from the thoracic cavity of 34 WTD. A true NAb prevalence of 88% was established from the serum samples. The FPS eluates performed most poorly (56% apparent prevalence; 57% sensitivity; 50% specificity), followed by serosanguinous fluid (88% apparent prevalence; 87% sensitivity; 0% specificity). Serosanguinous fluid appeared to better reflect the WTD population's true seroprevalence, although sensitivity decreased to <90% for both nonserum biological sample types. As studies are being developed to better understand the ecology of SARS-CoV-2 infections in WTD and other wildlife, it is important to consider the sample type being assessed, because sensitivity may vary greatly.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"726-731"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

B Cell Lymphoma in an Adult Female Mule Deer (Odocoileus hemionus) from Wyoming, USA. 美国怀俄明州成年母骡鹿B细胞淋巴瘤的研究。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-25-00034

María J Forzán, Jessica Jennings-Gaines, Eric R Burrough, Samantha E Allen

引用次数: 0

Eco-epidemiological Investigation of a Disease Outbreak among Pampas Foxes (Lycalopex gymnocercus) from a Protected Area in Southern Brazil. 巴西南部某保护区潘帕斯狐（Lycalopex gymnocercus）疾病暴发的生态流行病学调查

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-25-00004

José Reck, Greice Zorzato Gonchoroski, Maria Ogrzewalska, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge, Márcia Jardim, Tatiane C Trigo

{"title":"Eco-epidemiological Investigation of a Disease Outbreak among Pampas Foxes (Lycalopex gymnocercus) from a Protected Area in Southern Brazil.","authors":"José Reck, Greice Zorzato Gonchoroski, Maria Ogrzewalska, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge, Márcia Jardim, Tatiane C Trigo","doi":"10.7589/JWD-D-25-00004","DOIUrl":"10.7589/JWD-D-25-00004","url":null,"abstract":"Pathogens of domestic dogs have been reported in various carnivorans worldwide. Canine distemper virus (CDV) has been responsible for lethal outbreaks and population declines. Data are scarce regarding CDV outbreaks and their impact on South American canids. An eco-epidemiological investigation of a disease outbreak in a free-living population of Pampas foxes (Lycalopex gymnocercus) in a protected area (reserve) in southern Brazil began after locals informed our team of three foxes in the reserve showing signs of incoordination and seizures. Two carcasses of recently dead foxes were recovered and tested for CDV and rabies. Additionally, samples from 22 Pampas foxes live trapped in the study area were analyzed. Samples of the two dead foxes were positive for CDV antigen, and one of them for the presence of CDV RNA. None of the animals were positive for rabies virus. Analysis of a sequence from the CDV hemagglutinin gene allowed the classification of the CDV strain within the South America 1/Europe 1 (SA1/E1) clade, with high identity with other strains previously identified in domestic dogs. Approximately 90% of live-trapped Pampas foxes were seropositive for the presence of anti-CDV antibodies, and two of them showed myoclonus, indicating an outbreak of CDV in a population of free-ranging Pampas foxes in southern Brazil, possibly due to a spillover from domestic dogs.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"773-781"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatozoonosis in Free-Ranging American Mink (Neovison vison) in Pennsylvania, USA: Case Series. 美国宾夕法尼亚州自由放养的美国水貂（Neovison vison）的肝人畜共患病：病例系列。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00172

Lane Potts, Ian Gereg, Kayla B Garrett, Michael J Yabsley, Kevin D Niedringhaus

引用次数: 0

Comparison of Rapid Antigen Test and Quantitative PCR for Detection of Highly Pathogenic Avian Influenza Virus in Free-Ranging Peruvian Seabirds. 秘鲁散养海鸟高致病性禽流感病毒快速抗原试验与定量PCR检测的比较

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00188

Caitlin M Hemby, Matthew C Allender, Susana Cárdenas-Alayza, Sandipty Kayastha, Leyi Wang, Julie D Sheldon

{"title":"Comparison of Rapid Antigen Test and Quantitative PCR for Detection of Highly Pathogenic Avian Influenza Virus in Free-Ranging Peruvian Seabirds.","authors":"Caitlin M Hemby, Matthew C Allender, Susana Cárdenas-Alayza, Sandipty Kayastha, Leyi Wang, Julie D Sheldon","doi":"10.7589/JWD-D-24-00188","DOIUrl":"10.7589/JWD-D-24-00188","url":null,"abstract":"The recent highly pathogenic avian influenza (HPAI) outbreak in Peru devastated seabird populations and was associated with mass mortality events in marine mammals. The first mortality events were observed in coastal Peru in 2022, with the death of hundreds of Peruvian Pelicans (Pelecanus thagus). The outbreak response included health assessments and attempts to use rapid virus detection methods in the field on live and dead birds. Our study compared the FluDETECT Avian rapid antigen screening test that is commonly used in poultry with reverse transcription quantitative PCR (RT-qPCR) for the detection of HPAI virus in Peruvian seabirds. In total, 43 birds across five species were evaluated in this study: Humboldt Penguins (Spheniscus humboldti; n=6), Guanay Cormorants (Leucocarbo bougainvilliorum [Phalacrocorax bougainvillii]; n=19), Peruvian Pelicans (n=13), Kelp Gulls (Larus dominicanus; n=2), and Peruvian Boobies (Sula variegata; n=3). We did not find agreement between the results of the rapid antigen test and the RT-qPCR (Cohen kappa=0.14). The diagnostic sensitivity of the rapid test was low (33%), whereas the specificity was 100%. Based on these findings, we do not recommend the FluDETECT Avian rapid antigen test for influenza screening in seabird species, although given the high specificity, a positive result from this rapid test should be interpreted as a true positive.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"756-759"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0