Journal of Wildlife Diseases最新文献

Highly Pathogenic Avian Influenza Virus H5N1 in Double-crested Cormorants (Nannopterum auritum) of the Chesapeake Bay, USA.

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-02-06 DOI: 10.7589/JWD-D-24-00111

Johanna Harvey, Jeffery D Sullivan, Rebecca L Poulson, Deborah L Carter, Cindy P Driscoll, Peter C McGowan, Carl R Callahan, Amy W O'Donnell, Jennifer M Mullinax, David E Stallknecht, Diann J Prosser

{"title":"Highly Pathogenic Avian Influenza Virus H5N1 in Double-crested Cormorants (Nannopterum auritum) of the Chesapeake Bay, USA.","authors":"Johanna Harvey, Jeffery D Sullivan, Rebecca L Poulson, Deborah L Carter, Cindy P Driscoll, Peter C McGowan, Carl R Callahan, Amy W O'Donnell, Jennifer M Mullinax, David E Stallknecht, Diann J Prosser","doi":"10.7589/JWD-D-24-00111","DOIUrl":"https://doi.org/10.7589/JWD-D-24-00111","url":null,"abstract":"Double-crested Cormorants (Nannopterum auritum) have historically exhibited low levels of infection and antibodies to avian influenza virus (AIV). The recent global expansion of clade 2.3.4.4b A/goose/Guangdong/1/1996 highly pathogenic (HP) avian influenza virus H5N1 (HPAI H5N1) has resulted in large-scale mortalities across diverse waterbird taxa including cormorants. We sampled 32 and 29 Double-crested Cormorants breeding in the Chesapeake Bay, US, during the summers of 2023 and 2024, respectively, to assess HPAI H5N1 infection and AIV antibodies. Although no mortality was observed in the area, one bird sampled in 2023 was infected with HPAI H5N1. Additionally, 21/31 individuals in 2023 and 10/25 individuals in 2024 for which sera were collected had AIV antibodies. Based on additional testing using hemagglutination inhibition, virus neutralization, and an enzyme-linked lectin assay, 94 and 100% (2023 and 2024, respectively) of the seropositive birds tested positive for antibodies to both H5 and N1, suggesting previous infection with HPAI H5N1. These results are consistent with survival and limited clinical effects related to HPAI H5N1 infections. Furthermore, these results suggest that population immunity to HPAI H5N1 within the Chesapeake Bay might reduce future infections and potential population impacts should HP H5N1 remain on the landscape, though immunity may be waning across time. Because results are based on a single population, additional testing for both infection and antibodies as well as continued monitoring could enhance understanding of antibody persistence.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Packed Cell Volume and Plasma Chemistry Reference Intervals for Eurasian Eagle Owls (Bubo bubo) in a Rehabilitation Setting in South Korea before Release. 韩国欧亚鹰鸮放归前康复环境中的填料细胞体积和血浆化学参考区间。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00014

Hee-Jong Kim, Kyoo-Tae Kim

引用次数: 0

Serosurvey of Arboviruses in Free-Ranging Mantled Howler Monkeys (Alouatta palliata) in Costa Rica. 哥斯达黎加散养曼特吼猴（Alouatta palliata）的阿博病毒血清调查。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00082

R Scott Larsen, Anneke Moresco, Nick Karabatsos, Gaby Dolz, Kenneth E Glander

引用次数: 0

High Ectoparasite Loads of Tropical Birds: Chewing Lice on Puerto Rican American Kestrels (Falco sparverius caribaearum). 热带鸟类的高体外寄生虫负荷：波多黎各美洲隼（Falco sparverius caribaearum）身上的嚼虱。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00100

Martín G Frixione, Sarah E Bush, Dale H Clayton

{"title":"High Ectoparasite Loads of Tropical Birds: Chewing Lice on Puerto Rican American Kestrels (Falco sparverius caribaearum).","authors":"Martín G Frixione, Sarah E Bush, Dale H Clayton","doi":"10.7589/JWD-D-24-00100","DOIUrl":"10.7589/JWD-D-24-00100","url":null,"abstract":"Ectoparasite loads of birds may be governed, in part, by the climatic characteristics of their environment. We surveyed live-trapped American Kestrels (Falco sparverius caribaearum) for lice and other ectoparasites on the humid subtropical island of Puerto Rico during March-May 2024. The main goal was to compare the prevalence and abundance of lice on kestrels in Puerto Rico to recently published data on the lice of kestrels 1000 km away in the Bahamas, and to the lice of kestrels in an arid region of the western US (Utah). A brief general inspection of the plumage of 39 captured birds was followed by careful examination of the underside of wing primary feathers under a dissecting microscope. Two species of lice were collected, Colpocephalum subzerafae and Degeeriella carruthi, neither previously recorded from kestrels in Puerto Rico. The same two species are present on kestrels in the Bahamas. The prevalence and abundance of lice on kestrels in Puerto Rico and the Bahamas were similar, while being much greater than the prevalence and abundance of lice on kestrels in arid Utah, US. We also collected two species of hippoboscid flies (Diptera: Hippoboscidae): Microlynchia pusilla, not previously recorded on the American Kestrel, and Ornithoctona erythrocephala. These flies, as well as the lice, might affect the health of kestrels both directly, e.g., causing anemia by feeding on blood, and indirectly by vectoring endoparasites.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"241-246"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Klebsiella pneumoniae Complex-Associated Peritonitis, Lymphadenitis, and Pyelonephritis in Juvenile Raccoons (Procyon lotor) under Rehabilitator Care in New York, New Jersey, and Wisconsin, USA. 美国纽约、新泽西州和威斯康星州由康复者照料的幼年浣熊（Procyon lotor）的复合肺炎克雷伯氏菌相关腹膜炎、淋巴腺炎和肾盂肾炎。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00027

Zoe E Mack, Lorelei L Clarke, Elizabeth L Buckles, Rebecca J Franklin-Guild, Elena Alina Demeter

{"title":"Klebsiella pneumoniae Complex-Associated Peritonitis, Lymphadenitis, and Pyelonephritis in Juvenile Raccoons (Procyon lotor) under Rehabilitator Care in New York, New Jersey, and Wisconsin, USA.","authors":"Zoe E Mack, Lorelei L Clarke, Elizabeth L Buckles, Rebecca J Franklin-Guild, Elena Alina Demeter","doi":"10.7589/JWD-D-24-00027","DOIUrl":"10.7589/JWD-D-24-00027","url":null,"abstract":"Klebsiella spp. are gram-negative facultative anaerobic heavily encapsulated bacteria associated with opportunistic and primary infections in a wide range of species. We assessed a series of cases (n=8) of necrosuppurative peritonitis, lymphadenitis, and/or pyelonephritis in wild juvenile raccoons (Procyon lotor) that died under rehabilitator care in New York, New Jersey, and Wisconsin, US, between July 2020 and December 2023, plus a retrospective case of a juvenile raccoon necropsied from New York in August 2011. Gross necropsy (n=9) and histopathology (n=9) were performed to characterize the lesions, whereas bacterial culture (n=8) was used to identify and characterize the bacteria and associated phenotype. We observed gram-negative short rods and coccobacilli (7/9; 78%), fibrinosuppurative peritonitis of variable severity (7/9; 78%) correlated to gross pyoabdomen (5/9; 56%) or abscessation (2/9; 22%), lymphadenomegaly and associated necrosuppurative lymphadenitis (5/9; 56%), and urinary tract disease (3/9; 33%). Aerobic culture of affected tissues isolated Klebsiella pneumoniae (n=4), K. pneumoniae subsp. ozaenae (n=2), Klebsiella variicola (n=1), and Klebsiella sp. (n=1). Our study strongly suggests an association of bacteria within the K. pneumoniae complex with peritonitis, lymphadenitis, and pyelonephritis in raccoons. Disease might be associated with underlying nosocomial infection given that all animals were under rehabilitator care at the time of death.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"173-179"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomal Micro RNA Isolation in White-Tailed Deer (Odocoileus virginianus) for Diagnostic Biomarker Discovery. 白尾鹿（Odocoileus virginianus）外泌体微小 RNA 分离用于诊断生物标记物的发现。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00075

Maite De María, Lillian G Maxwell, Margaret E Hunter, Jason A Ferrante

{"title":"Exosomal Micro RNA Isolation in White-Tailed Deer (Odocoileus virginianus) for Diagnostic Biomarker Discovery.","authors":"Maite De María, Lillian G Maxwell, Margaret E Hunter, Jason A Ferrante","doi":"10.7589/JWD-D-24-00075","DOIUrl":"10.7589/JWD-D-24-00075","url":null,"abstract":"Molecular approaches are becoming more prevalent for the diagnosis of neurodegenerative diseases in human medicine and can be extended to diagnosis of wildlife diseases such as chronic wasting disease and other prion diseases. These diseases have been associated with exosome-bound molecular biomarkers of disease progression, such as proteins and micro RNA molecules (miRNA). We tested and optimized a method for exosomal miRNA isolation from minimally invasive, small-volume serum samples obtained from white-tailed deer (Odocoileus virginianus). We confirmed the isolation of exosomes and optimized a commercially available benchtop kit to obtain sufficient and pure RNA for miRNA sequencing. The selected method for RNA extraction combines two 500-μL serum aliquots into one elution column and re-eluting the final product of the column. We identified 137 miRNA present in healthy white-tailed deer that can be used as a baseline to identify putative miRNA biomarkers of disease progression and mechanisms of infection in future comparative disease studies. This approach to biomarker discovery may help to inform biological processes in wildlife populations and provide alternatives to invasive or postmortem samples.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"212-218"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Prevalence of Sarcocystis in a Collapsed Black Rat (Rattus rattus) Population from the Florida Keys, Florida, USA. 佛罗里达礁岛群塌陷黑鼠（Rattus rattus）中沙囊虫的高流行率。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00041

Brandon M McDonald, Michael V Cove, Mark G Ruder, Michael J Yabsley, Kayla B Garrett, Alec T Thompson, Nicole M Nemeth, Jeremy D Dixon, Marcus A Lashley

{"title":"High Prevalence of Sarcocystis in a Collapsed Black Rat (Rattus rattus) Population from the Florida Keys, Florida, USA.","authors":"Brandon M McDonald, Michael V Cove, Mark G Ruder, Michael J Yabsley, Kayla B Garrett, Alec T Thompson, Nicole M Nemeth, Jeremy D Dixon, Marcus A Lashley","doi":"10.7589/JWD-D-24-00041","DOIUrl":"10.7589/JWD-D-24-00041","url":null,"abstract":"We collected and screened black rats (Rattus rattus) in Key Largo, Florida, USA, to determine the potential role of disease or parasites in the collapse of the local population. Rats appeared healthy, but 94% (n=15/16) tested positive for Sarcocystis sp. The partial 18S rRNA gene sequence was 98.7-99.7% similar to a strain of Sarcocystis zuoi that is now considered a strain of the newly described Sarcocystis kani within the larger S. zuoi species complex that contains numerous new species. These Sarcocystis spp. use Asian snakes as definitive hosts and rodents, shrews, or tree shrews as intermediate hosts. Pythons are the definitive host for several Sarcocystis spp. in Asia, including a related parasite (Sarcocystis singaporensis) that has been used as a biologic control agent for Rattus spp. in southeast Asia. It is probable that increasing numbers of invasive Burmese pythons (Python bivittatus) in the Florida Keys are contributing to the spread of this parasite in addition to imposing predation pressure on both rodents and native snakes. As such, further surveillance and molecular and morphologic characterization of parasites from rodents and snakes in south Florida should be prioritized.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"180-185"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Ostertagia mossi and Ostertagia dikmansi from White-Tailed Deer (Odocoileus virginianus) Confirms they are the Same Species. 从白尾鹿（Odocoileus virginianus）中提取的 Ostertagia mossi 和 Ostertagia dikmansi 的分子特征证实它们是同一物种。

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00096

C Anderson Smith, Elizabeth A Kurimo-Beechuk, Kayla B Garrett, Mark G Ruder, Ethan P Barton, Michael J Yabsley

引用次数: 0

Evaluation of RT-QuIC Diagnostic Performance for Chronic Wasting Disease Detection Using Elk (Cervus canadensis) Ear Punches. 利用麋鹿耳孔检测慢性消耗性疾病的RT-QuIC诊断性能评价

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00071

Damani N Bryant, Roxanne J Larsen, Kristin J Bondo, Andrew S Norton, Andrew J Lindbloom, Steven L Griffin, Peter A Larsen, Tiffany M Wolf, Stuart S Lichtenberg

{"title":"Evaluation of RT-QuIC Diagnostic Performance for Chronic Wasting Disease Detection Using Elk (Cervus canadensis) Ear Punches.","authors":"Damani N Bryant, Roxanne J Larsen, Kristin J Bondo, Andrew S Norton, Andrew J Lindbloom, Steven L Griffin, Peter A Larsen, Tiffany M Wolf, Stuart S Lichtenberg","doi":"10.7589/JWD-D-24-00071","DOIUrl":"10.7589/JWD-D-24-00071","url":null,"abstract":"Sensitive and specific antemortem diagnostic tests are a prerequisite for effective management of chronic wasting disease (CWD). Paired with readily accessible samples that accurately reflect CWD status, the real-time quaking-induced conversion (RT-QuIC) assay has the potential to enable more effective CWD surveillance and interventions. We evaluated the feasibility of RT-QuIC as a CWD diagnostic test using 6-mm ear tissue biopsies from elk (Cervus canadensis). First, we evaluated the effect of ear spatial location on seeding activity. We observed an effect of ear punch spatial location on the amyloid formation rate (AFR): Samples collected from the periphery of the ear evidenced a statistically significant increase in AFR relative to ear punches from the ventral midline. Gross microdissection of an ear pinna suggested that there was more small nerve innervation around the periphery of the ear. Second, we evaluated the diagnostic sensitivity, specificity, and predictive value of RT-QuIC using ear punches from elk that had been previously diagnosed via ELISA testing. We evaluated the impact of nonstatistical and statistical approaches on diagnostic accuracy. Specificity and positive predictive value were perfect when statistical analyses were used to evaluate the binomial distribution (CWD positive versus CWD negative) of the data. Conversely, sensitivity and negative predictive value were modest, independent of the application of statistical analysis, indicating that RT-QuIC may be susceptible to false-negative data in this context. Taken together, our data support the idea that RT-QuIC, when paired with US Department of Agriculture-approved diagnostic tests, may provide more time to stakeholders for making major management decisions.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"64-75"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serologic Evidence of Exposure to Leishmania infantum in Captive and Free-Ranging European Bison (Bison bonasus) in Poland, 2017-23 . 2017-23年波兰圈养和自由放养欧洲野牛（Bison bonasus）暴露于幼年利什曼原虫的血清学证据

IF 1.1 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-01-01 DOI: 10.7589/JWD-D-24-00032

Anna Didkowska, Víctor Martín-Santander, Marlena Wojciechowska, Wanda Olech, Krzysztof Anusz, Antonio Fernández, Janine E Davies, Marta Ruíz de Arcaute, Delia Lacasta, Sergio Villanueva-Saz, Diana Marteles

{"title":"Serologic Evidence of Exposure to Leishmania infantum in Captive and Free-Ranging European Bison (Bison bonasus) in Poland, 2017-23 .","authors":"Anna Didkowska, Víctor Martín-Santander, Marlena Wojciechowska, Wanda Olech, Krzysztof Anusz, Antonio Fernández, Janine E Davies, Marta Ruíz de Arcaute, Delia Lacasta, Sergio Villanueva-Saz, Diana Marteles","doi":"10.7589/JWD-D-24-00032","DOIUrl":"10.7589/JWD-D-24-00032","url":null,"abstract":"The European Bison (Bison bonasus) is the largest mammal in Europe and is classified as an endangered species. Leishmaniosis is a vector-borne disease caused by the protozoan Leishmania infantum. In general, this infection has been associated with dogs, cats, and humans. However, epidemiologic studies and reports confirm that the parasite is able to infect many other mammalian species. Recent evidence has demonstrated that ruminants in endemic areas are exposed to L. infantum infection. Moreover, climate change has allowed the northward spread of vector species, causing the expansion of L. infantum infection in regions traditionally classified as nonendemic in Europe. The aim of this study was to determine the presence or absence of anti-L. infantum antibodies in serum samples from 343 European bison in Poland, collected from 2017 to 2023. For this purpose, the presence of anti-Leishmania antibodies was analyzed using an in-house multispecies ELISA. Anti-Leishmania antibodies were detected in four animals, an overall seroprevalence of 1.17%. The results provide scientific evidence of serologic exposure to the parasite in Poland, a country previously considered nonendemic for L. infantum infection.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"253-257"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0