Journal of Wildlife Diseases最新文献_第2页

Multi-year Occurrence of Ranavirus and Batrachochytrium dendrobatidis in Anurans from Central, Southeast, and Coastal Regions of Texas, USA, 2012-19. 2012-2019年美国德克萨斯州中部、东南部和沿海地区阿纳兰病毒和树突壶菌的多年流行

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00186

Andrea Villamizar-Gomez, Trina Guerra, Shashwat Sirsi, William L Farr, Michael R J Forstner, Dittmar Hahn

{"title":"Multi-year Occurrence of Ranavirus and Batrachochytrium dendrobatidis in Anurans from Central, Southeast, and Coastal Regions of Texas, USA, 2012-19.","authors":"Andrea Villamizar-Gomez, Trina Guerra, Shashwat Sirsi, William L Farr, Michael R J Forstner, Dittmar Hahn","doi":"10.7589/JWD-D-24-00186","DOIUrl":"10.7589/JWD-D-24-00186","url":null,"abstract":"Ranaviruses (Iridoviridae) and chytrid fungus (Batrachochytrium spp.) cause globally reportable diseases affecting a wide array of amphibians. Both pathogens are well documented in the US and have caused local or regional scale mortality events for susceptible amphibians. Over a period of 8 y (2012-2019), we tested salvaged amphibians collected during amphibian audio survey work across the central, southeastern, and coastal areas of Texas, US. Individuals of 7/8 anuran taxa tested positive for either or both pathogens, as did individuals in counties with large sample sizes (i.e., n>25) across taxa. Ranavirus prevalence was high in 2012 (67%) but decreased to 5% by 2019. Conversely, chytrid fungus prevalence started low in 2013 (4%) but increased to 20% by 2019. The detection of both pathogens in any individual was consistently rare throughout the period. Although salvage of specimens during annual fieldwork adds permitting requirements, handling time, and curation effort, we argue that this additional effort significantly contributes to regional pathogen surveillance. Therefore, it should be considered a standard approach for the extensive array of roadway-based herpetofaunal surveys conducted each year.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"749-755"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dogs can Generalize from Cotton Training Aids to Fecal Matter in Chronic Wasting Disease Detection. 犬在慢性消耗性疾病的检测中可以从棉花训练辅助物到粪便进行推广。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00093

Amritha Mallikarjun, Clara Wilson, Ila Charendoff, Madison B Moore, Elizabeth Nguyen, Abigail J Hendrzak, Michelle Gibison, Cynthia M Otto

{"title":"Dogs can Generalize from Cotton Training Aids to Fecal Matter in Chronic Wasting Disease Detection.","authors":"Amritha Mallikarjun, Clara Wilson, Ila Charendoff, Madison B Moore, Elizabeth Nguyen, Abigail J Hendrzak, Michelle Gibison, Cynthia M Otto","doi":"10.7589/JWD-D-24-00093","DOIUrl":"10.7589/JWD-D-24-00093","url":null,"abstract":"Chronic wasting disease (CWD) is a prion-associated transmissible spongiform encephalopathy (TSE) that causes fatal neurodegeneration in cervids. One promising method for CWD surveillance is the use of detection dogs trained to differentiate between fecal matter from CWD-positive (infected) and CWD-negative (noninfected) deer. However, using actual fecal matter during the training process confers a biohazard risk. Chronic wasting disease detection dogs trained on fecal matter can differentiate between CWD-positive and CWD-negative training aids, including cotton aids, which are inexpensive and simple to use. However, as most dogs in an applied setting would instead be trained on aids and tasked with finding actual fecal matter in the field, this study examined 1) the extent to which dogs initially trained on CWD-incubated cotton can discriminate between fecal samples from CWD-positive and CWD-negative deer; and 2) the impact of substrate form (cotton ball or cotton roll) on the dogs' detection accuracy. Results show that dogs (n=5) maintained an accuracy of >80% during training while discriminating between cotton ball training aids that had been incubated (noncontact) with deer fecal samples confirmed as either CWD-positive or CWD-negative. When tested with cotton ball training aids incubated with novel samples, dogs (n=4) showed 73% sensitivity and 77% specificity. When presented with deer fecal samples, dogs showed a sensitivity of 50% and a specificity of 90%. The type of cotton (ball or roll) did not significantly influence performance. In sum, cotton aids can be used to train dogs to detect the CWD odor profile effectively. However, an initial reduction in sensitivity suggests that integrating exposure to target fecal matter may enhance detection performance.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"600-608"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Prevalence of Antigens of and Specific Antibodies Against Various Viral Pathogens in European Wildcats (Felis silvestris) from Southwest Germany, 2020-22. 2020-2022年德国西南部欧洲野猫（Felis silvestris）各种病毒病原体抗原和特异性抗体的高流行率

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00191

Sarah Pauline Stubbe, Johannes Lang, Nicole Nagler, Simon Franz Müller, Michael Lierz

{"title":"High Prevalence of Antigens of and Specific Antibodies Against Various Viral Pathogens in European Wildcats (Felis silvestris) from Southwest Germany, 2020-22.","authors":"Sarah Pauline Stubbe, Johannes Lang, Nicole Nagler, Simon Franz Müller, Michael Lierz","doi":"10.7589/JWD-D-24-00191","DOIUrl":"10.7589/JWD-D-24-00191","url":null,"abstract":"The recent expansion of the European wildcat (Felis silvestris silvestris) and increasing numbers of domestic cats in Germany are leading to more interactions between domestic cats and wildcats. Not only hybridization, but also the reciprocal transmission of pathogens may occur. This could threaten wildcat populations, or they may act as a reservoir for their domestic relatives. In this study, 102 effusion fluid samples collected from wildcats found dead between 2020 and 2022 in the German federal state of Rhineland-Palatinate were serologically tested for antigens of and specific antibodies against viral pathogens typically found in domestic cats (feline herpesvirus [FHV], feline calicivirus [FCV], feline parvovirus [FPV], feline immunodeficiency virus [FIV], feline coronavirus [FCoV], and feline leukemia virus [FeLV]). Antigens of and/or specific antibodies against at least one virus were detected in 59% of the wildcats. Detected prevalences of specific antibodies against FHV (22.5%), FCV (20.6%), FPV (13.7%), FIV (0%), and FCoV (17.6%) were each 0-22.5%. The prevalence of antigens of FeLV was 28.4%. The high prevalence detected for FeLV antigens possibly shows that wildcats form a reservoir for this virus, as the prevalence in domestic cat populations is significantly lower.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"663-673"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pansteatitis in Wild American Alligators (Alligator mississippiensis). 野生美洲短吻鳄（密西西比短吻鳄）的泛咽喉炎。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00190

Miriam N Boucher, Justin M Stilwell, Marisa Tellez, Shane M Boylan, Thomas R Rainwater, Stefanie L Whitmire, James T Anderson

引用次数: 0

The Efficacy of Surrogate Virus Neutralization Test ELISA Varies in the Detection of SARS-CoV-2 Antibodies with Different Postmortem White-Tailed Deer (Odocoileus virginianus) Biological Samples. 替代病毒中和试验ELISA检测不同白尾鹿死后生物标本SARS-CoV-2抗体的效果差异

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00155

Raquel Francisco, Sonia M Hernandez, Ethan P Barton, Melanie R Kunkel, Kayla G Adcock, Daniel G Mead, Mark G Ruder, Jillian R Broadhurst, Michael J Yabsley

{"title":"The Efficacy of Surrogate Virus Neutralization Test ELISA Varies in the Detection of SARS-CoV-2 Antibodies with Different Postmortem White-Tailed Deer (Odocoileus virginianus) Biological Samples.","authors":"Raquel Francisco, Sonia M Hernandez, Ethan P Barton, Melanie R Kunkel, Kayla G Adcock, Daniel G Mead, Mark G Ruder, Jillian R Broadhurst, Michael J Yabsley","doi":"10.7589/JWD-D-24-00155","DOIUrl":"10.7589/JWD-D-24-00155","url":null,"abstract":"The susceptibility of white-tailed deer (WTD; Odocoileus virginianus) to SARS-CoV-2 has resulted in WTD being one of the most studied wildlife species during the COVID-19 pandemic. However, WTD specimen collections for SARS-CoV-2 research and surveillance have largely been opportunistic. Our objective was to evaluate the detection of SARS-CoV-2 neutralizing antibodies (NAbs) in WTD serum, plus two additional WTD biological sample types, using a blocking ELISA previously used for WTD serum. The two additional sample types evaluated were eluted Nobuto filter-paper strips (FPSs) from heparinized whole blood collected perimortem from 34 WTD and serosanguinous fluid collected postmortem from the thoracic cavity of 34 WTD. A true NAb prevalence of 88% was established from the serum samples. The FPS eluates performed most poorly (56% apparent prevalence; 57% sensitivity; 50% specificity), followed by serosanguinous fluid (88% apparent prevalence; 87% sensitivity; 0% specificity). Serosanguinous fluid appeared to better reflect the WTD population's true seroprevalence, although sensitivity decreased to <90% for both nonserum biological sample types. As studies are being developed to better understand the ecology of SARS-CoV-2 infections in WTD and other wildlife, it is important to consider the sample type being assessed, because sensitivity may vary greatly.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"726-731"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

B Cell Lymphoma in an Adult Female Mule Deer (Odocoileus hemionus) from Wyoming, USA. 美国怀俄明州成年母骡鹿B细胞淋巴瘤的研究。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-25-00034

María J Forzán, Jessica Jennings-Gaines, Eric R Burrough, Samantha E Allen

引用次数: 0

Eco-epidemiological Investigation of a Disease Outbreak among Pampas Foxes (Lycalopex gymnocercus) from a Protected Area in Southern Brazil. 巴西南部某保护区潘帕斯狐（Lycalopex gymnocercus）疾病暴发的生态流行病学调查

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-25-00004

José Reck, Greice Zorzato Gonchoroski, Maria Ogrzewalska, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge, Márcia Jardim, Tatiane C Trigo

{"title":"Eco-epidemiological Investigation of a Disease Outbreak among Pampas Foxes (Lycalopex gymnocercus) from a Protected Area in Southern Brazil.","authors":"José Reck, Greice Zorzato Gonchoroski, Maria Ogrzewalska, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge, Márcia Jardim, Tatiane C Trigo","doi":"10.7589/JWD-D-25-00004","DOIUrl":"10.7589/JWD-D-25-00004","url":null,"abstract":"Pathogens of domestic dogs have been reported in various carnivorans worldwide. Canine distemper virus (CDV) has been responsible for lethal outbreaks and population declines. Data are scarce regarding CDV outbreaks and their impact on South American canids. An eco-epidemiological investigation of a disease outbreak in a free-living population of Pampas foxes (Lycalopex gymnocercus) in a protected area (reserve) in southern Brazil began after locals informed our team of three foxes in the reserve showing signs of incoordination and seizures. Two carcasses of recently dead foxes were recovered and tested for CDV and rabies. Additionally, samples from 22 Pampas foxes live trapped in the study area were analyzed. Samples of the two dead foxes were positive for CDV antigen, and one of them for the presence of CDV RNA. None of the animals were positive for rabies virus. Analysis of a sequence from the CDV hemagglutinin gene allowed the classification of the CDV strain within the South America 1/Europe 1 (SA1/E1) clade, with high identity with other strains previously identified in domestic dogs. Approximately 90% of live-trapped Pampas foxes were seropositive for the presence of anti-CDV antibodies, and two of them showed myoclonus, indicating an outbreak of CDV in a population of free-ranging Pampas foxes in southern Brazil, possibly due to a spillover from domestic dogs.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"773-781"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatozoonosis in Free-Ranging American Mink (Neovison vison) in Pennsylvania, USA: Case Series. 美国宾夕法尼亚州自由放养的美国水貂（Neovison vison）的肝人畜共患病：病例系列。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00172

Lane Potts, Ian Gereg, Kayla B Garrett, Michael J Yabsley, Kevin D Niedringhaus

引用次数: 0

Comparison of Rapid Antigen Test and Quantitative PCR for Detection of Highly Pathogenic Avian Influenza Virus in Free-Ranging Peruvian Seabirds. 秘鲁散养海鸟高致病性禽流感病毒快速抗原试验与定量PCR检测的比较

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00188

Caitlin M Hemby, Matthew C Allender, Susana Cárdenas-Alayza, Sandipty Kayastha, Leyi Wang, Julie D Sheldon

{"title":"Comparison of Rapid Antigen Test and Quantitative PCR for Detection of Highly Pathogenic Avian Influenza Virus in Free-Ranging Peruvian Seabirds.","authors":"Caitlin M Hemby, Matthew C Allender, Susana Cárdenas-Alayza, Sandipty Kayastha, Leyi Wang, Julie D Sheldon","doi":"10.7589/JWD-D-24-00188","DOIUrl":"10.7589/JWD-D-24-00188","url":null,"abstract":"The recent highly pathogenic avian influenza (HPAI) outbreak in Peru devastated seabird populations and was associated with mass mortality events in marine mammals. The first mortality events were observed in coastal Peru in 2022, with the death of hundreds of Peruvian Pelicans (Pelecanus thagus). The outbreak response included health assessments and attempts to use rapid virus detection methods in the field on live and dead birds. Our study compared the FluDETECT Avian rapid antigen screening test that is commonly used in poultry with reverse transcription quantitative PCR (RT-qPCR) for the detection of HPAI virus in Peruvian seabirds. In total, 43 birds across five species were evaluated in this study: Humboldt Penguins (Spheniscus humboldti; n=6), Guanay Cormorants (Leucocarbo bougainvilliorum [Phalacrocorax bougainvillii]; n=19), Peruvian Pelicans (n=13), Kelp Gulls (Larus dominicanus; n=2), and Peruvian Boobies (Sula variegata; n=3). We did not find agreement between the results of the rapid antigen test and the RT-qPCR (Cohen kappa=0.14). The diagnostic sensitivity of the rapid test was low (33%), whereas the specificity was 100%. Based on these findings, we do not recommend the FluDETECT Avian rapid antigen test for influenza screening in seabird species, although given the high specificity, a positive result from this rapid test should be interpreted as a true positive.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"756-759"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Multivariate Analyses to Explore Host-Pathogen Coevolution in Complex Trait Space. 利用多变量分析探索复杂性状空间中的宿主-病原体协同进化。

IF 1.2 4区农林科学

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI: 10.7589/JWD-D-24-00123

Rachel M Ruden, Amberleigh E Henschen, Marissa M Langager, Dana M Hawley, James S Adelman

{"title":"Using Multivariate Analyses to Explore Host-Pathogen Coevolution in Complex Trait Space.","authors":"Rachel M Ruden, Amberleigh E Henschen, Marissa M Langager, Dana M Hawley, James S Adelman","doi":"10.7589/JWD-D-24-00123","DOIUrl":"10.7589/JWD-D-24-00123","url":null,"abstract":"Lesion severity scores are often used to monitor individual health outcomes following the incursion of certain wildlife diseases. However, collapsing a complex trait such as pathology onto a single axis can mask critical information about host-pathogen interactions. In this study, we apply multivariate techniques (shape and community analyses) to explore potential patterns of coevolution in a well-studied wildlife disease system: House Finches (Haemorhous mexicanus) infected with Mycoplasma gallisepticum, a bacterium that causes conjunctival pathology that is visible and facilitates transmission. We captured hatch-year House Finches from two USA populations that differ in their history of pathogen exposure: a Virginia population that has experienced seasonal epizootics for >25 yr and a Hawaii population that is naïve to the pathogen. We then experimentally infected the birds with one of two isolates that varied in virulence. The Virginia birds showed milder distortions of the eye rim, reflected as shorter distances traveled through disease space, across isolates than did the Hawaii birds. Although birds expressed an overlapping suite of pathologic descriptors, the high-virulence isolate caused Virginia birds to express certain pathologies at different frequencies, leading to depauperate communities, compared with the Hawaii birds in which pathologies were expressed more evenly. Notably, eversion was expressed in nearly half of all Virginia eye-days (number of days an eye was sampled) with pathology in response to the high-virulence isolate despite relatively mild lesion severity scores. This may indicate that pathologies that can enhance host competence without compromising host fitness will be maintained and even selected for during host-pathogen coevolution, especially in wildlife populations trending toward disease tolerance.","PeriodicalId":17602,"journal":{"name":"Journal of Wildlife Diseases","volume":" ","pages":"609-618"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0