Journal of Veterinary Science最新文献

{"title":"Cervical spine reconstruction after total vertebrectomy using customized three-dimensional-printed implants in dogs.","authors":"Ji-Won Jeon, Kyu-Won Kang, Woo-Keyoung Kim, Sook Yang, Byung-Jae Kang","doi":"10.4142/jvs.23241","DOIUrl":"10.4142/jvs.23241","url":null,"abstract":"<p><strong>Background: </strong>Sufficient surgical resection is necessary for effective tumor control, but is usually limited for vertebral tumors, especially in the cervical spine in small animal neurosurgery.</p><p><strong>Objective: </strong>To evaluate the primary stability and safety of customized three-dimensional (3D)-printed implants for cervical spine reconstruction after total vertebrectomy.</p><p><strong>Methods: </strong>Customized guides and implants were designed based on computed tomography (CT) imaging of five beagle cadavers and were 3D-printed. They were used to reconstruct C5 after total vertebrectomy. Postoperative CT images were obtained to evaluate the safety and accuracy of screw positioning. After harvesting 10 vertebral specimens (C3-C7) from intact (group A) and implanted spines (group B), implant stability was analyzed using a 4-point bending test comparing with groups A and C (reconstituted with plate and pins/polymethylmethacrylate after testing in Group A).</p><p><strong>Results: </strong>All customized implants were applied without gross neurovascular damage. In addition, 90% of the screws were in a safe area, with 7.5% in grade 1 (< 1.3 mm) and 2.5% in grade 2 (> 1.3 mm). The mean entry point and angular deviations were 0.81 ± 0.43 mm and 6.50 ± 5.11°, respectively. Groups B and C significantly decreased the range of motion (ROM) in C3-C7 compared with intact spines (<i>p</i> = 0.033, and 0.018). Both groups reduced overall ROM and neutral zone in C4-C6, but only group B showed significance (<i>p</i> = 0.005, and 0.027).</p><p><strong>Conclusion: </strong>Customized 3D-printed implants could safely and accurately replace a cervical vertebra in dog cadavers while providing primary stability.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined antimicrobial effect of two peptide nucleic acids against <i>Staphylococcus aureus</i> and <i>S. pseudintermedius</i> veterinary isolates.","authors":"Se Kye Kim, Jun Bong Lee, Hyung Tae Lee, Jang Won Yoon","doi":"10.4142/jvs.23265","DOIUrl":"10.4142/jvs.23265","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> and <i>S. pseudintermedius</i> are the major etiological agents of staphylococcal infections in humans, livestock, and companion animals. The misuse of antimicrobial drugs has led to the emergence of antimicrobial-resistant <i>Staphylococcus</i> spp., including methicillin-resistant <i>S. aureus</i> (MRSA) and methicillin-resistant <i>S. pseudintermedius</i> (MRSP). One novel therapeutic approach against MRSA and MRSP is a peptide nucleic acid (PNA) that can bind to the target nucleotide strands and block expression. Previously, two PNAs conjugated with cell-penetrating peptides (P-PNAs), antisense PNA (ASP)-cmk and ASP-deoD, targeting two essential genes in <i>S. aureus</i>, were constructed, and their antibacterial activities were analyzed.</p><p><strong>Objectives: </strong>This study analyzed the combined antibacterial effects of P-PNAs on <i>S. aureus</i> and <i>S. pseudintermedius</i> clinical isolates.</p><p><strong>Methods: </strong><i>S. aureus</i> ATCC 29740 cells were treated simultaneously with serially diluted ASP-cmk and ASP-deoD, and the minimal inhibitory concentrations (MICs) were measured. The combined P-PNA mixture was then treated with <i>S. aureus</i> and <i>S. pseudintermedius</i> veterinary isolates at the determined MIC, and the antibacterial effect was examined.</p><p><strong>Results: </strong>The combined treatment of two P-PNAs showed higher antibacterial activity than the individual treatments. The MICs of two individual P-PNAs were 20 and 25 μM, whereas that of the combined treatment was 10 μM. The application of a combined treatment to clinical <i>Staphylococcus</i> spp. revealed <i>S. aureus</i> isolates to be resistant to P-PNAs and <i>S. pseudintermedius</i> isolates to be susceptible.</p><p><strong>Conclusions: </strong>These observations highlight the complexity of designing ASPs with high efficacy for potential applications in treating staphylococcal infections in humans and animals.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-like lymphoplasmacytic conjunctivitis in the third eyelid in a dog.","authors":"Hyeonu Sung, Jisoo Park, Jury Kim, Seonmi Kang, Gillian C Shaw, Kangmoon Seo","doi":"10.4142/jvs.23222","DOIUrl":"10.4142/jvs.23222","url":null,"abstract":"<p><p>This report aims to describe a case of tumor-like lymphoplasmacytic conjunctivitis in a 7-year-old spayed-female Pomeranian. On complete ophthalmic examination, a mass with papillary projections was noted on the bulbar surface of the right third eyelid. Debulking of the mass was performed while preserving as much of the third eyelid as possible. On the histopathological examination, the mass was diagnosed as lymphoplasmacytic conjunctivitis with mild epithelial hyperplasia. Although a slight regrowth of the mass was noted 3 weeks after surgery, intralesional injection of triamcinolone acetonide led to its disappearance. There was no further recurrence after 5 months.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis.","authors":"Samah Attia Algharib, Ali Sobhy Dawood, Lingli Huang, Aizhen Guo, Gang Zhao, Kaixiang Zhou, Chao Li, Jinhuan Liu, Xin Gao, Wanhe Luo, Shuyu Xie","doi":"10.4142/jvs.23147","DOIUrl":"10.4142/jvs.23147","url":null,"abstract":"<p><p>Mastitis is one of the most widespread infectious diseases that adversely affects the profitability of the dairy industry worldwide. Accurate diagnosis and identification of pathogens early to cull infected animals and minimize the spread of infection in herds is critical for improving treatment effects and dairy farm welfare. The major pathogens causing mastitis and pathogenesis are assessed first. The most recent and advanced strategies for detecting mastitis, including genomics and proteomics approaches, are then evaluated . Finally, the advantages and disadvantages of each technique, potential research directions, and future perspectives are reported. This review provides a theoretical basis to help veterinarians select the most sensitive, specific, and cost-effective approach for detecting bovine mastitis early.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring preventive factors against insufficient antibody positivity rate for foot-and-mouth disease in pig farms in South Korea: a preliminary ecological study.","authors":"Dongwoon Han, Byeongwoo Ahn, Kyung-Duk Min","doi":"10.4142/jvs.23185","DOIUrl":"10.4142/jvs.23185","url":null,"abstract":"<p><strong>Background: </strong>Foot-and-mouth disease (FMD) is a highly contagious viral disease in livestock that has tremendous economic impact nationally. After multiple FMD outbreaks, the South Korean government implemented a vaccination policy for efficient disease control. However, during active surveillance by quarantine authorities, pig farms have reported an insufficient antibody positivity rate to FMD.</p><p><strong>Objective: </strong>In this study, the spatial and temporal trends of insufficiency among pig farms were analyzed, and the effect of the number of government veterinary officers was explored as a potential preventive factor.</p><p><strong>Methods: </strong>Various data were acquired, including national-level surveillance data for antibody insufficiency from the Korea Animal Health Integrated System, the number of veterinary officers, and the number of local pig farms. Temporal and geographical descriptive analyses were conducted to overview spatial and temporal trends. Additionally, logistic regression models were employed to investigate the association between the number of officers per pig farm with antibody insufficiency. Spatial cluster analysis was conducted to detect spatial clusters.</p><p><strong>Results: </strong>The results showed that the incidence of insufficiency tended to decrease in recent years (odds ratio [OR], 0.803; 95% confidence interval [95% CIs], 0.721-0.893), and regions with a higher density of governmental veterinary officers (OR, 0.942; 95% CIs, 0.918-0.965) were associated with a lower incidence.</p><p><strong>Conclusions: </strong>This study implies that previously conducted national interventions would be effective, and the quality of government-provided veterinary care could play an important role in addressing the insufficient positivity rate of antibodies.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intramammary preparation of enrofloxacin hydrochloride-dihydrate for bovine mastitis (biofilm-forming <i>Staphylococcus aureus)</i>.","authors":"Diana Córdova-González, Edgar Alfonseca-Silva, Lilia Gutiérrez, Graciela Tapia-Pérez, Héctor Sumano","doi":"10.4142/jvs.23245","DOIUrl":"10.4142/jvs.23245","url":null,"abstract":"<p><strong>Background: </strong>Chronic bovine mastitis is linked to biofilm-producing <i>Staphylococcus aureus</i> (bp-Sa) or <i>Staphylococcus</i> coagulase-negative (bp-Scn).</p><p><strong>Objectives: </strong>Bp-Sa and bp-Scn were treated with intramammary preparations of either enrofloxacin HCl·2H₂O-dimethyl-sulfoxide-chitosan (enro-C/DMSO/chitosan) or enro-C alone. Their potential to inhibit and degrade biofilm formation <i>in vitro</i> was also assessed.</p><p><strong>Methods: </strong>Milk samples were obtained from the affected quarters in a herd. Phenotypical and genotypical identifications as biofilm-producing <i>Staphylococcus</i> species were carried out. Enro-C/DMSO/chitosan and enro-C alone were assessed to determine their <i>in vitro</i> efficacy in interfering with biofilm formation and their bactericidal effects. A prolonged eight-day treatment with a twice-daily intramammary insertion of 10 mL of enro-C/DMSO/chitosan or enro-C alone was set to evaluate the clinical and bacteriological cures on day 10 in 15 cows per group and the biofilm-inhibiting ability.</p><p><strong>Results: </strong>Fifty-seven percent of the isolates were identified as <i>Staphylococcus</i> spp., of which 50% were bp-Sa, 46% bp-Scn, and 4% <i>Staphylococcus pseudintermedius</i>. One hundred percent of the <i>S. aureus</i> isolated and 77% of <i>Staphylococcus</i> coagulase-negative were biofilm producers. In both groups, the <i>icaA</i> and <i>icaD</i> biofilm-producing genes were identified. The experimental preparation could inhibit biofilm formation, degrade mature biofilms, and have well-defined microbicidal effects on planktonic and biofilm bacteria. The respective clinical and bacteriological cure rates were 100% and 80% for enro-C/DMSO/chitosan and 41.7% and 25% for enro-C alone.</p><p><strong>Conclusions: </strong>Enro-C/DMSO/chitosan eliminates bp-Sa and bp-Scn from cases of chronic bovine mastitis.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> evaluation of the antitumor activity of axitinib in canine mammary gland tumor cell lines.","authors":"Hye-Gyu Lee, Ga-Hyun Lim, Ju-Hyun An, Su-Min Park, Kyoung-Won Seo, Hwa-Young Youn","doi":"10.4142/jvs.23191","DOIUrl":"10.4142/jvs.23191","url":null,"abstract":"<p><strong>Background: </strong>Axitinib, a potent and selective inhibitor of vascular endothelial growth factor (VEGF) receptor (VEGFR) tyrosine kinase 1,2 and 3, is used in chemotherapy because it inhibits tumor angiogenesis by blocking the VEGF/VEGFR pathway. In veterinary medicine, attempts have been made to apply tyrosine kinase inhibitors with anti-angiogenic effects to tumor patients, but there are no studies on axitinib in canine mammary gland tumors (MGTs).</p><p><strong>Objectives: </strong>This study aimed to confirm the antitumor activity of axitinib in canine mammary gland cell lines.</p><p><strong>Methods: </strong>We treated canine MGT cell lines (CIPp and CIPm) with axitinib and conducted CCK, wound healing, apoptosis, and cell cycle assays. Additionally, we evaluated the expression levels of angiogenesis-associated factors, including <i>VEGFs</i>, <i>PDGF-A</i>, <i>FGF-2</i>, and <i>TGF-β1</i>, using quantitative real-time polymerase chain reaction. Furthermore, we collected canine peripheral blood mononuclear cells (PBMCs), activated them with concanavalin A (ConA) and lipopolysaccharide (LPS), and then treated them with axitinib to investigate changes in viability.</p><p><strong>Results: </strong>When axitinib was administered to CIPp and CIPm, cell viability significantly decreased at 24, 48, and 72 h (<i>p</i> < 0.001), and migration was markedly reduced (6 h, <i>p</i> < 0.05; 12 h, <i>p</i> < 0.005). The apoptosis rate significantly increased (<i>p</i> < 0.01), and the G2/M phase ratio showed a significant increase (<i>p</i> < 0.001). Additionally, there was no significant change in the viability of canine PBMCs treated with LPS and ConA.</p><p><strong>Conclusion: </strong>In this study, we confirmed the antitumor activity of axitinib against canine MGT cell lines. Accordingly, we suggest that axitinib can be applied as a new treatment for patients with canine MGTs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Salmonella</i> vector induces protective immunity against <i>Lawsonia</i> and <i>Salmonella</i> in murine model using prokaryotic expression system.","authors":"Sungwoo Park, Eunseok Cho, Amal Senevirathne, Hak-Jae Chung, Seungmin Ha, Chae-Hyun Kim, Seogjin Kang, John Hwa Lee","doi":"10.4142/jvs.23219","DOIUrl":"10.4142/jvs.23219","url":null,"abstract":"<p><strong>Background: </strong><i>Lawsonia intracellularis</i> is the causative agent of proliferative enteropathy and is associated with several outbreaks, causing substantial economic loss to the porcine industry.</p><p><strong>Objectives: </strong>In this study, we focused on demonstrating the protective effect in the mouse model through the immunological bases of two vaccine strains against porcine proliferative enteritis.</p><p><strong>Methods: </strong>We used live-attenuated <i>Salmonella</i> Typhimurium (ST) secreting two selected immunogenic LI antigens (<i>Lawsonia</i> autotransporter A epitopes and flagellin [FliC]-peptidoglycan-associated lipoprotein-FliC) as the vaccine carrier. The constructs were cloned into a <i>Salmonella</i> expression vector (pJHL65) and transformed into the ST strain (JOL912). The expression of immunogenic proteins within <i>Salmonella</i> was evaluated via immunoblotting.</p><p><strong>Results: </strong>Immunizing BALB/c mice orally and subcutaneously induced high levels of LI-specific systemic immunoglobulin G and mucosal secretory immunoglobulin A. In immunized mice, there was significant upregulation of interferon-γ and interleukin-4 cytokine mRNA and an increase in the subpopulations of cluster of differentiation (CD) 4⁺ and CD 8⁺ T lymphocytes upon splenocytes re-stimulation with LI antigens. We observed significant protection in C57BL/6 mice against challenge with 10^6.9 times the median tissue culture infectious dose of LI or 2 × 10⁹ colony-forming units of the virulent ST strain. Immunizing mice with either individual vaccine strains or co-mixture inhibited bacterial proliferation, with a marked reduction in the percentage of mice shedding <i>Lawsonia</i> in their feces.</p><p><strong>Conclusions: </strong><i>Salmonella</i>-mediated LI gene delivery induces robust humoral and cellular immune reactions, leading to significant protection against LI and salmonellosis.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139692158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution and international transmission of H3N2 canine influenza A viruses from Korea during 2014-2017.","authors":"Chung-Young Lee","doi":"10.4142/jvs.23105","DOIUrl":"10.4142/jvs.23105","url":null,"abstract":"<p><p>Avian-origin H3N2 canine influenza A viruses (CIVs) have become enzootic in China and Korea and have sporadically transmitted to North America, causing multiple epidemics. We isolated six CIVs in Korea from CIV-infected patients during 2014-2017 and conducted whole genome sequencing and phylogenetic analyses. Results revealed that CIVs have circulated and evolved in Korea since the early 2000s and then diversified into a new clade, probably contributing to multiple epidemics in China, the USA, and Canada. Our findings bridge an evolutionary gap for understanding the global transmission of CIVs, emphasizing the significance of continuous monitoring of CIVs.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71412734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous hypersensitivity reactions to trimethoprim-sulfamethoxazole and amoxicillin-clavulanate in a dog.","authors":"Yunho Jeong, Yoon-Hwan Kim, Jin-Ok Ahn, Jin-Young Chung","doi":"10.4142/jvs.23188","DOIUrl":"10.4142/jvs.23188","url":null,"abstract":"<p><p>Antibiotics are known to be able to cause hypersensitivity reactions through various mechanisms. We present a case of drug-induced immune thrombocytopenia (DITP) and anaphylactic shock occurring simultaneously in a dog after the administration of two classes of antibiotics, namely trimethoprim-sulfamethoxazole (TMP-SMX) and amoxicillin-clavulanate (AMC). The patient recovered completely from DITP on discontinuation of TMP-SMX and the anaphylactic shock caused by AMC was treated with intensive care. DITP is a rare adverse drug reaction (ADR), and anaphylactic shock is a life-threatening ADR. This is the first case report of a dog manifesting two types of hypersensitivity reactions caused by two antibiotics.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10694372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71412746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}