Journal of Vascular Research最新文献_第8页

Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis. Willis小鼠血管平滑肌细胞的分离和培养。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2023-01-01 Epub Date: 2023-08-29 DOI: 10.1159/000532033

Wei Chang, Yajuan Li, Fengzhou Liu, Kehai Zang, Peiran Zhang, Shuai Qu, Jingyu Zhao, Junhui Xue

{"title":"Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis.","authors":"Wei Chang, Yajuan Li, Fengzhou Liu, Kehai Zang, Peiran Zhang, Shuai Qu, Jingyu Zhao, Junhui Xue","doi":"10.1159/000532033","DOIUrl":"10.1159/000532033","url":null,"abstract":"Introduction: Culturing cerebrovascular smooth muscle cells (CVSMCs) in vitro can provide a model for studying many cerebrovascular diseases. This study describes a convenient and efficient method to obtain mouse CVSMCs by enzyme digestion.Methods: Mouse circle of Willis was isolated, digested, and cultured with platelet-derived growth factor-BB (PDGF-BB) to promote CVSMC growth, and CVSMCs were identified by morphology, immunofluorescence analysis, and flow cytometry. The effect of PDGF-BB on vascular smooth muscle cell (VSMC) proliferation was evaluated by cell counting kit (CCK)-8 assay, morphological observations, Western blotting, and flow cytometry.Results: CVSMCs cultured in a PDGF-BB-free culture medium had a typical peak-to-valley growth pattern after approximately 14 days. Immunofluorescence staining and flow cytometry detected strong positive expression of the cell type-specific markers alpha-smooth muscle actin (α-SMA), smooth muscle myosin heavy chain 11 (SMMHC), smooth muscle protein 22 (SM22), calponin, and desmin. In the CCK-8 assay and Western blotting, cells incubated with PDGF-BB had significantly enhanced proliferation compared to those without PDGF-BB.Conclusion: We obtained highly purified VSMCs from the mouse circle of Willis using simple methods, providing experimental materials for studying the pathogenesis and treatment of neurovascular diseases in vitro. Moreover, the experimental efficiency improved with PDGF-BB, shortening the cell cultivation period.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"234-244"},"PeriodicalIF":1.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10109932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Preliminary Discussion on the Safety of Mild Therapeutic Hypothermia in Target Vessels after Endovascular Intervention in Acute Large Vessel Occlusion Cerebral Infarction. 急性大血管闭塞性脑梗死血管内介入治疗后靶血管亚低温治疗的安全性初探。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2023-01-01 Epub Date: 2023-08-28 DOI: 10.1159/000532030

Jiang Li, Shaonian Tang, Juanli Liu, Wenlin He, Jinjin Yan, Zhiyong Huang, Xuesong Li

{"title":"A Preliminary Discussion on the Safety of Mild Therapeutic Hypothermia in Target Vessels after Endovascular Intervention in Acute Large Vessel Occlusion Cerebral Infarction.","authors":"Jiang Li, Shaonian Tang, Juanli Liu, Wenlin He, Jinjin Yan, Zhiyong Huang, Xuesong Li","doi":"10.1159/000532030","DOIUrl":"10.1159/000532030","url":null,"abstract":"Introduction: The aim of this study was to discuss the safety of rapid administration of 4°C hypothermic normal saline into the occluded vessels using an intra-arterial catheter to induce mild hypothermia following endovascular thrombectomy in patients with acute large vessel occlusion cerebral infarction.Methods: We selected 78 patients with acute large vessel occlusion cerebral infarction who underwent endovascular thrombectomy in the Department of Neurology of our hospital from January 2020 to July 2022 and achieved TICI 2b recanalization.Result: Twenty-five patients were administered 500 mL of 4°C hypothermic normal saline in the occluded vessels at a rate of 25 mL/min to induce mild hypothermia. Twenty pairs of subjects conformed to strict matching and were finally included in the statistical analysis. The two groups of patients differed significantly in white blood cell count and percentage of neutrophils (p < 0.05); however, there were no significant differences in D-dimer, procalcitonin, and BNP levels. The two groups of patients did not differ significantly with respect to the incidence of the following indicators: upper gastrointestinal bleeding; pulmonary infection; venous thrombosis; vasospasms; seizures; and chills (p > 0.05).Conclusion: Mild therapeutic hypothermia in target vessels plus endovascular thrombectomy was shown to be safe in patients with acute large vessel occlusion cerebral infarction.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"227-233"},"PeriodicalIF":1.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress. Cx43促进剪切应力诱导的内皮细胞间充质转化。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2023-01-01 Epub Date: 2023-09-06 DOI: 10.1159/000533320

En Zhou, Jing Zhou, Changlong Bi, Zongqi Zhang

{"title":"Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress.","authors":"En Zhou, Jing Zhou, Changlong Bi, Zongqi Zhang","doi":"10.1159/000533320","DOIUrl":"10.1159/000533320","url":null,"abstract":"Objectives: This study aimed to determine the function of Cx43 in the endothelial-to-mesenchymal transition (EndMT) process of endothelial cells (ECs) and to explore the potential signaling pathways underlying these functions.Methods: ECs were extracted from rat aorta. ECs were transfected with Cx43 cDNA and Cx43 siRNA and then exposed to 5 or 12 dyne/cm2. Immunofluorescence staining was used to detect the expression of SM22α, Cx43, and acetylated α-tubulin in ECs. Western blotting was used to detect the protein expression of α-SMA, CD31, Cx43, H1-calponin, Ift88, and p-smad3 in ECs.Results: The expression of αSMA, SM22α, and Cx43 was significantly increased, and CD31 was markedly decreased in ECs treated with laminar shear stress at 5 dyn/cm2. The Cx43 cDNA transfection could induce the expression of SM22α or H1-calponin and attenuate CD31 expression in ECs. Also, Cx43 overexpression harms cilia formation in ECs exposed to 5 dyn/cm2, accompanied with the regulated Ift88 and smad signaling.Conclusions: This study found that laminar shear stress at 5 dyn/cm2 would increase the expression of Cx43 to facilitate the EndMT process of ECs, associated with morphological changes in primary cilia and the decreased expression of Ift88 in ECs.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"204-212"},"PeriodicalIF":1.7,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10614473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10226346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thanks to the Reviewers 感谢审稿人

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-12-21 DOI: 10.1159/000527829

引用次数: 0

Front & Back Matter 正面和背面

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-10-01 DOI: 10.1159/000527666

引用次数: 0

Front & Back Matter 正面和背面事项

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-07-01 DOI: 10.1159/000526085

C. Garland, A. Heagerty

引用次数: 0

Perivascular Adipose Tissue Compensation for Endothelial Dysfunction in the Superior Mesenteric Artery of Female SHRSP.Z-Leprfa/IzmDmcr Rats 血管周围脂肪组织对雌性SHRSP.Z-Leprf/IzmDmcr大鼠肠系膜上动脉内皮功能障碍的补偿

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-04-29 DOI: 10.1159/000524187

S. Kagota, Risa Futokoro, Kana Maruyama-Fumoto, J. McGuire, K. Shinozuka

{"title":"Perivascular Adipose Tissue Compensation for Endothelial Dysfunction in the Superior Mesenteric Artery of Female SHRSP.Z-Leprfa/IzmDmcr Rats","authors":"S. Kagota, Risa Futokoro, Kana Maruyama-Fumoto, J. McGuire, K. Shinozuka","doi":"10.1159/000524187","DOIUrl":"https://doi.org/10.1159/000524187","url":null,"abstract":"Regulation of arterial tone by perivascular adipose tissue (PVAT) differs between sexes. In male SHRSP.Z-Leprfa/IzmDmcr rats (SHRSP.ZF), PVAT exerts a compensatory relaxation effect for the loss of endothelium-mediated vasorelaxation, which occurs during the early stages of metabolic syndrome. However, this effect deteriorates by 23 weeks of age. Here, therefore, we compared the effects of PVAT in female and male SHRSP.ZF. Acetylcholine-induced relaxation in superior mesenteric artery without PVAT did not differ between 23-week-old females and males. However, the presence of PVAT enhanced relaxation in 23-week-old females, but not in males. The mRNA levels of angiotensin II type 1 receptor (AT1R) in PVAT did not differ between sexes, but AT1R-associated protein (ATRAP) and apelin levels were higher in females than in males. We observed a positive relationship between differences in artery relaxation with and without PVAT and ATRAP or apelin mRNA levels. In 30-week-old females, PVAT-enhanced relaxation disappeared, and mRNA levels of AT1R increased, while apelin levels decreased compared to 23-week-old females. These results demonstrated that in SHRSP.ZF, PVAT compensation for endothelium dysfunction extended to older ages in females than in males. Apelin and AT1R/ATRAP expression in PVAT may be predictors of favorable effects.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":"59 1","pages":"209 - 220"},"PeriodicalIF":1.7,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43713316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vivo Evidence of Arterial Dynamic Properties Alteration in Atherosclerotic Rabbit 动脉粥样硬化兔动脉动态特性改变的体内证据

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-04-19 DOI: 10.1159/000523898

C. Vayssettes-Courchay, C. Ragonnet, M. Isabelle, M. Bourguignon, S. Chimenti

{"title":"In vivo Evidence of Arterial Dynamic Properties Alteration in Atherosclerotic Rabbit","authors":"C. Vayssettes-Courchay, C. Ragonnet, M. Isabelle, M. Bourguignon, S. Chimenti","doi":"10.1159/000523898","DOIUrl":"https://doi.org/10.1159/000523898","url":null,"abstract":"Objectives: Atherosclerosis severely damages the arterial wall. The aim of this study was to assess in vivo, for the first time, arterial dynamic properties, reactivity, and stiffness in atherosclerotic (ATH) rabbits. Methods: The rabbits were fed with 0.3% cholesterol diet. Femoral artery (FA) or abdominal aorta (AA) diameter was recorded by echotracking, together with blood pressure. Arterial reactivity after local administration of agents and stiffness were measured as diameter or pulsatile diameter changes. Results: FA dilation induced by acetylcholine was reduced in the function of diet duration (9–65 weeks). With mid-term diet duration (35–45 weeks), the dilation to nitroprusside was greatly reduced; the constriction to norepinephrine was reduced but not that to serotonin, thromboxane agonist, or angiotensin II. After 17- and 28-week diet AA and FA stiffness were increased while distensibility was reduced. Arterial stiffness measured by regional pulse wave velocity was unaltered. We observed that after 28-week diet, FA exhibited a stiffened wall at the plaque level and higher distensibility at the upstream site. Discussion/Conclusion: Arterial reactivity and compliance were greatly modified by atherosclerosis, at various degrees dependent on diet duration. ATH rabbit is therefore a suitable model for in vivo investigations of treatments targeting dynamic properties of arterial wall.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":"59 1","pages":"239 - 250"},"PeriodicalIF":1.7,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46359861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of Zebrafish Models for Diabetes Mellitus and Its Microvascular Complications 斑马鱼糖尿病及其微血管并发症模型的建立

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-04-04 DOI: 10.1159/000522471

Changsheng Chen, Dong Liu

引用次数: 4

Front & Back Matter 正面和背面

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2022-03-01 DOI: 10.1159/000524211

C. Wit, A. Heagerty

引用次数: 0