Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress.

IF 1.8 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

Journal of Vascular Research Pub Date : 2023-01-01 Epub Date: 2023-09-06 DOI:10.1159/000533320

En Zhou, Jing Zhou, Changlong Bi, Zongqi Zhang

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引用次数: 0

Abstract

Objectives: This study aimed to determine the function of Cx43 in the endothelial-to-mesenchymal transition (EndMT) process of endothelial cells (ECs) and to explore the potential signaling pathways underlying these functions.

Methods: ECs were extracted from rat aorta. ECs were transfected with Cx43 cDNA and Cx43 siRNA and then exposed to 5 or 12 dyne/cm2. Immunofluorescence staining was used to detect the expression of SM22α, Cx43, and acetylated α-tubulin in ECs. Western blotting was used to detect the protein expression of α-SMA, CD31, Cx43, H1-calponin, Ift88, and p-smad3 in ECs.

Results: The expression of αSMA, SM22α, and Cx43 was significantly increased, and CD31 was markedly decreased in ECs treated with laminar shear stress at 5 dyn/cm2. The Cx43 cDNA transfection could induce the expression of SM22α or H1-calponin and attenuate CD31 expression in ECs. Also, Cx43 overexpression harms cilia formation in ECs exposed to 5 dyn/cm2, accompanied with the regulated Ift88 and smad signaling.

Conclusions: This study found that laminar shear stress at 5 dyn/cm2 would increase the expression of Cx43 to facilitate the EndMT process of ECs, associated with morphological changes in primary cilia and the decreased expression of Ift88 in ECs.

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Cx43促进剪切应力诱导的内皮细胞间充质转化。

目的：本研究旨在确定Cx43在内皮细胞（EC）内皮-间充质转化（EndMT）过程中的功能，并探索这些功能背后的潜在信号通路。方法：从大鼠主动脉中提取内皮细胞。用Cx43 cDNA和Cx43 siRNA转染EC，然后暴露于5或12达因/cm2。免疫荧光染色法检测内皮细胞中SM22α、Cx43和乙酰化α-微管蛋白的表达。采用蛋白质印迹法检测α-SMA、CD31、Cx43、H1钙蛋白酶、Ift88和p-smad3在内皮细胞中的蛋白表达。Cx43cDNA转染可诱导内皮细胞中SM22α或H1钙蛋白酶的表达，并减弱CD31的表达。此外，Cx43过表达损害暴露于5dyn/cm2的EC的纤毛形成，并伴有调节的Ift88和smad信号传导。结论：本研究发现，5dyn/cm2的层流剪切应力会增加Cx43的表达，以促进内皮细胞的EndMT过程，这与原发纤毛的形态学变化和内皮细胞中Ift88的表达减少有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vascular Research 医学-生理学

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.