Journal of Vascular Research最新文献

{"title":"Expression of Desmocollin-1 in carotid atherosclerotic plaques and calcific aortic valves.","authors":"Kashif Khan, Bin Yu, Sarah Abdulkarim, Adam Schwertani, Jacques Genest, Stella S Daskalopoulou, Adel Schwertani","doi":"10.1159/jvr/adiag006","DOIUrl":"https://doi.org/10.1159/jvr/adiag006","url":null,"abstract":"<p><strong>Introduction: </strong>Atherosclerosis and calcific aortic valve stenosis are active, regulated processes sharing features such as lipid infiltration, extracellular matrix deposition, cellular proliferation, and calcification. Their exact mechanisms remain unclear. High-density lipoprotein (HDL) is central to reverse cholesterol transport, and Desmocollin-1 (DSC1) has recently been implicated in HDL biogenesis. This study assessed DSC1 expression and localization in atherosclerotic plaques and calcific aortic valves.</p><p><strong>Methods: </strong>Human carotid artery specimens (19 subjects: plaques and normal tissue) and aortic valves (50 subjects: stenotic calcific and normal) were collected. DSC1 mRNA expression was measured by quantitative RT-PCR, and protein expression was evaluated using immunohistochemistry and Western blot analyses.</p><p><strong>Results: </strong>DSC1 mRNA and protein were abundantly expressed in carotid plaques and stenotic calcific valves, but not in normal tissues. In calcified valves, DSC1 localized to lipid cores, inflammatory cells, and microvessels. In carotid plaques, DSC1 was mainly found in foam cells and myointimal cells.</p><p><strong>Conclusion: </strong>DSC1 expression is significantly increased in both atherosclerotic plaques and stenotic calcific valves, suggesting a role in lipid metabolism abnormalities. These findings point to DSC1 as a potential contributor to disease pathogenesis and warrant further targeted investigation.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red Blood Cell Dynamics in Healthy and Cancer-Influenced Venular Flow: Role of Flow Conditions and Cell Stiffness.","authors":"Tahereh Zarei, Cyrus Aghanajafi, M Soltani","doi":"10.1159/jvr/adiag007","DOIUrl":"https://doi.org/10.1159/jvr/adiag007","url":null,"abstract":"<p><strong>Background: </strong>Hemodynamic alterations in microvessels under pathological conditions such as cancer can modify RBC dynamics and near-wall behavior, influencing the cell-free layer and local flow microenvironment.</p><p><strong>Methods: </strong>Three-dimensional simulations of two red blood cells with different stiffness levels were performed in venular flow under conditions representing a healthy individual and a breast cancer patient. The simulations were carried out using a force-coupling immersed-boundary lattice Boltzmann framework. RBC deformation, longitudinal and radial migration, velocity distribution, shear rate, and pressure fields were analyzed using hematocrit data, with blood viscosity derived from these data.</p><p><strong>Results: </strong>Compared with healthy flow, cancer-affected conditions led to reduced RBC deformation and diminished radial migration, resulting in increased near-wall residence. This effect was more pronounced for the stiffened RBC. It was accompanied by a local reduction in near-wall velocity, an increase in shear rate, and larger pressure differences across the cell. Shear rate was higher in cancer-affected flow, while near-wall variations were reduced.</p><p><strong>Conclusions: </strong>The results indicate that cancer-associated changes in venular flow promote RBC near-wall localization and may suppress CFL formation, primarily due to reduced RBC deformation, overriding low-hematocrit dilution. Circulating tumor cells were not modeled; however, RBC dynamics suggest a microenvironment that may facilitate near-wall interactions.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149149582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Prophylactic Negative Pressure Therapy in the Murine Hindlimb Lymphedema Model.","authors":"Shahnur Ahmed, Ganesh Mohan, Daniel J Konig, Christopher A Subi-Kasozi, Steven Sullivan, Angad S Sidhu, Miguel D Jorge, Helen C Sinex, Marc S Mendonca, Arin K Greene, Mithun Sinha, Aladdin H Hassanein","doi":"10.1159/jvr/adiag004","DOIUrl":"10.1159/jvr/adiag004","url":null,"abstract":"<p><strong>Introduction: </strong>Lymphedema is limb swelling secondary to lymphatic injury. Utilization of negative pressure therapy (NPT) has been shown to stimulate lymphatic vessel density, but the effect of NPT on secondary lymphedema is unknown. The purpose of this study was to evaluate the role of prophylactic NPT in murine hindlimb lymphedema model.</p><p><strong>Methods: </strong>The murine hindlimb model of lymphedema was used. Mice underwent 20-Gy irradiation of one hindlimb. One-week post-radiation, an incision in the proximal hindlimb was created to disrupt lymphatic vessels and remove popliteal lymph nodes. Mice were divided into two groups: group 1 (NPT) and group 2 (no NPT). Group 1 received a negative pressure of 75 mm Hg on the lymphedema-induced hindlimb for 12 consecutive hours in the first 5 postoperative days (PODs). Group 2 did not receive NPT. Paw thickness measurements and near-infrared laser lymphangiography were measured.</p><p><strong>Results: </strong>The study included 16 mice (group 1 n = 6, group 2 n = 10). Paw thickness on POD 21 in group 1 was 2.9 ± 0.3 mm compared to 3.3 ± 0.3 mm in group 2 (p = 0.02). On POD 35, the average paw thickness was 2.4 ± 0.1 mm in group 1 compared to 3.2 ± 0.2 mm (p = 0.001). Lymphangiography dye clearance was greater in group 1 compared to group 2 (p = 0.008).</p><p><strong>Conclusions: </strong>Mice in the hindlimb lymphedema model that underwent NPT in the early postoperative period had less paw thickness swelling and better lymphatic function than mice that did not have NPT. NPT decreased lymphedema when used prophylactically following lymphatic injury.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial-Fibroblast Organoids Generated in Hydrogel Microchamber Arrays: A Translational Model of Vascular Dysfunction in Cardio-Kidney-Metabolic Disease.","authors":"Tali Zitman-Gal, Maria Sobolev, Anna Talash, Shelly Tartakover Matalon, Guy Topaz, Jacob Chen, David Pereg, Gloria Rashid, Naomi Nacasch, Arik Dahan, Mordechai Deutsch, Keren Cohen-Hagai","doi":"10.1159/jvr/adiag003","DOIUrl":"https://doi.org/10.1159/jvr/adiag003","url":null,"abstract":"<p><p>Cardio-kidney-metabolic disease comprises interconnected cardiovascular, renal, and metabolic disorders in which endothelial dysfunction drives microvascular and macrovascular injury. Although novel treatments improve clinical outcomes, the cellular and molecular mechanisms underlying these effects remain incompletely understood. We developed and characterized a three-dimensional (3D) endothelial-fibroblast co-culture platform to study endothelial morphogenesis and responses to metabolic and pharmacological stimuli.</p><p><strong>Methods: </strong>A hydrogel microchamber array (HMC) platform was used to generate reproducible endothelial spheroids and extracellular matrix embedded organoids. Endothelial cells were cultured alone or with fibroblasts. Morphometric changes were quantified over time following glucose exposure and treatment with the GLP-1 receptor agonist/insulin combination (Xultophy).</p><p><strong>Results: </strong>The HMC platform enabled reproducible formation of spheroids and organoids. Fibroblast co-culture enhanced endothelial organization and structural complexity. Glucose induced organoid expansion and reduced circularity, consistent with structural remodeling. Xultophy attenuated these changes and preserved organoid morphology. Responses were dependent on the sequence of stimulation, with partial mitigation of glucose-induced effects by Xultophy. Organoids demonstrated greater sensitivity than spheroids, highlighting the importance of extracellular matrix context.</p><p><strong>Conclusions: </strong>The HMC platform provides a reproducible and physiologically relevant 3D system for investigating endothelial-stromal interactions, metabolic stress, and therapeutic modulation in cardio-kidney-metabolic disease.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Regulation, Metabolic, Inflammatory, and Hormone Signaling RNA Transcriptomic Pathways are Upregulated among Patients with Coronary Microvascular Dysfunction and Ischemia with Nonobstructive Coronary Arteries.","authors":"Barbara Jerome, Florencia Schlamp, Anaïs Hausvater, Amanda Joa, Claudia Serrano-Gomez, Ayman Farid, Tessa Barrett, Harmony R Reynolds, Jeffrey S Berger, Nathaniel R Smilowitz","doi":"10.1159/jvr/adiag002","DOIUrl":"10.1159/jvr/adiag002","url":null,"abstract":"<p><strong>Introduction: </strong>Mechanisms underlying coronary microvascular dysfunction (CMD), a known cause of ischemia with nonobstructive coronary arteries (INOCA), are not well understood.</p><p><strong>Methods: </strong>In a prospective cohort study, we investigated the whole-blood transcriptome of 28 female patients with INOCA who underwent clinically indicated invasive coronary angiography to identify pathways relevant to the pathophysiology of CMD.</p><p><strong>Results: </strong>In this study, four protein-coding genes related to cell signaling and metabolism were differentially expressed and 47 transcriptomic pathways related to cell signaling, metabolism, inflammation, and sex hormone signaling were primarily upregulated with greater CMD.</p><p><strong>Conclusion: </strong>Our findings support the hypothesis that inflammation leads to maladaptive microvascular remodeling and fibrosis, contributing to the pathogenesis of CMD.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Immediate Effect of Applying Transcutaneous Electrical Nerve Stimulation on Sympathetic Ganglion and Gastrocnemius for Popliteal Blood Flow in Healthy Adults.","authors":"Meng-Jie Shen, Chia-Ling Lee, Ya-Ju Chang, Miao-Ju Hsu","doi":"10.1159/jvr/adiag005","DOIUrl":"10.1159/jvr/adiag005","url":null,"abstract":"<p><strong>Introduction: </strong>The influence of transcutaneous electrical nerve stimulation (TENS) site and frequency on lower limb hemodynamics remains unclear. This study was to examine the acute hemodynamic effects of high-frequency (80 Hz) and low-frequency (4 Hz) TENS applied to the sympathetic ganglion or gastrocnemius muscle in healthy adults.</p><p><strong>Methods: </strong>Forty-three healthy adults underwent four TENS protocols combining sympathetic ganglion or gastrocnemius stimulation with high or low frequency. Doppler ultrasonography measured popliteal artery and vein hemodynamics at baseline, during stimulation, immediately after, and 15 min post-stimulation, including vessel diameter (D), flow volume (FV), peak velocity (PV), and TAmax.</p><p><strong>Results: </strong>All four protocols significantly enhanced popliteal arterial and venous hemodynamics during stimulation (p < 0.001). Most parameters returned to baseline after stimulation. However, low-frequency TENS applied to the sympathetic ganglion produced sustained increases in arterial PV (p = 0.031) and a borderline increase in arterial FV (p = 0.050) compared with high-frequency stimulation, with no between-frequency differences in venous parameters (p > 0.05). With gastrocnemius stimulation, popliteal arterial and venous responses were frequency-independent for most parameters (p > 0.05).</p><p><strong>Conclusion: </strong>Both high- and low-frequency TENS applied to the sympathetic ganglion and gastrocnemius muscles acutely enhanced lower limb hemodynamics. Low-frequency sympathetic ganglion stimulation may induce sustained arterial effects.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148945195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of Endothelialised Vascular Grafts: A Bibliometric Analysis for Surgical Innovation.","authors":"Ahmad Hafiz Murtadha, Muhammad Dain Yazid, Nur Najmi Mohamad Anuar, Mohamad Fikeri Ishak, Nadiah Sulaiman","doi":"10.1159/jvr/adiag001","DOIUrl":"10.1159/jvr/adiag001","url":null,"abstract":"<p><strong>Introduction: </strong>Rapid endothelialisation is crucial for developing hemocompatible vascular grafts for coronary artery disease. This bibliometric analysis maps the research landscape of endothelial cells and endothelialisation in tissue-engineered vascular grafts (TEVGs) to identify evolving trends and surgical relevance.</p><p><strong>Methods: </strong>We analysed 1991 publications (2000-2024) from Web of Science using Bibliometrix, VOSviewer, and CiteSpace. Patent and clinical trial data were obtained from <ext-link ext-link-type=\"uri\" xlink:href=\"http://Lens.org\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">Lens.org</ext-link> and <ext-link ext-link-type=\"uri\" xlink:href=\"http://ClinicalTrials.gov\" xmlns:xlink=\"http://www.w3.org/1999/xlink\">ClinicalTrials.gov</ext-link>.</p><p><strong>Results: </strong>Publication output peaked in 2016 (119 articles), then declined post-2020. The USA led in volume (559 articles; 34,186 citations), while Singapore had the highest citation impact (119.9 average citations). Core themes included endothelialisation, angiogenesis, mesenchymal stem cells, and vascular endothelial growth factor. Early focus on prostheses and in vitro models declined, while 3D bioprinting, gelMA scaffolds, and surface modification emerged. Surface modification was identified as the primary research hotspot, with small-diameter TEVGs remaining the central translational focus. Patent activity decreased notably after 2016, and no clinical trials specifically linking TEVGs with endothelial cells were identified.</p><p><strong>Conclusion: </strong>Endothelialisation remains essential in TEVG research but is increasingly integrated into broader scaffold fabrication and validation strategies. The field is shifting toward biofabrication approaches like 3D bioprinting and functional evaluation platforms. However, significant translational gaps persist between laboratory research and clinical implementation.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1"},"PeriodicalIF":1.3,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic Inflammatory Indices Predict Survival and Limb Outcomes after Revascularization for Chronic Limb-Threatening Ischemia.","authors":"Franco Imbrognio, Ignacio Pepe, Alfredo Verastegui, Oliver A Gomez-Gutierrez, Oscar A De la Torre, Rocio Gau, Diego Herrera-Vegas, Mario Alejandro Fabiani","doi":"10.1159/000553405","DOIUrl":"10.1159/000553405","url":null,"abstract":"<p><strong>Introduction: </strong>Chronic limb-threatening ischemia (CLTI) carries high morbidity and mortality, yet risk stratification tools after revascularization remain limited. The Systemic Immune-Inflammation Index (SII) and Systemic Inflammatory Response Index (SIRI) have emerged as prognostic markers. This study evaluated their predictive value in CLTI patients undergoing revascularization.</p><p><strong>Methods: </strong>This retrospective single-center cohort study included 122 CLTI patients undergoing endovascular angioplasty (n = 81) or surgical bypass (n = 41) between 2017 and 2023. Pre-procedural SII and SIRI were calculated from blood counts. Outcomes included: overall survival, major adverse cardiovascular and limb events (MACE/MALE), and limb amputation. Kaplan-Meier analysis, Cox regression with Firth's penalized likelihood adjustment, and C-index analyses were performed.</p><p><strong>Results: </strong>Surgical bypass was associated with longer median survival than angioplasty (48 vs. 27 months, p = 0.012). Patients in the lowest SII quartile (<492) had significantly improved 84-month survival versus higher quartiles (88.4% vs. 55.1%). Elevated SII remained independently associated with mortality on multivariable analysis. SIRI was higher among patients with adverse events (2.7 vs. 2.0, p = 0.024) but was not independently associated with outcomes. SII demonstrated moderate-to-high predictive performance, with C-indices up to 0.921 in the bypass subgroup.</p><p><strong>Conclusion: </strong>Pre-procedural SII is independently associated with survival following CLTI revascularization and may aid risk stratification.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1-17"},"PeriodicalIF":1.3,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13493090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative Abdominal Aortic Calcification Morphology Is Associated with Lumen Geometry.","authors":"Gianna Elci, Zoraiz Bukhari, Pasani Dharmasena, Sayuri Sayakkara, Lionel Chong, Gursimran Singh, Sumona Rahman, Parsa Nadali, Kengo Kuwama, Matthew Duazo, Alvin Varghese, Kelsi Hurdle, Brian Lee Beatty","doi":"10.1159/000553274","DOIUrl":"10.1159/000553274","url":null,"abstract":"<p><strong>Introduction: </strong>Calcification morphology is increasingly studied in coronary arteries but remains poorly characterized in the abdominal aorta. Whether morphology provides information beyond total calcium burden is unclear. We used micro-computed tomography (micro-CT) with near-histologic resolution to characterize abdominal aortic calcification morphology and evaluate its relationship with local lumen geometry.</p><p><strong>Methods: </strong>Abdominal aortic specimens from 19 cadavers were imaged using micro-CT, yielding 26 lesions and 201 cross-sectional slices. Calcification was classified as concentric or eccentric based on circumferential arc. Measurements included calcium thickness, area, and arc, along with lumen area. Associations were assessed using linear mixed-effects models to account for clustering within lesions and specimens (effect estimates reported as β).</p><p><strong>Results: </strong>Lumen area did not differ by morphologic classification. Greater calcium thickness (β [95% CI] = -10.076 [-16.539, -3.613]; p = 0.002), area (β = -0.403 [-0.803, -0.003]; p = 0.049), and arc (β = -0.080 [-0.138, -0.022]; p = 0.007) were each associated with smaller lumen area. In sensitivity analyses, associations for thickness and arc remained significant, whereas the association for area was attenuated.</p><p><strong>Conclusion: </strong>Quantitative morphologic features were inversely associated with lumen area, suggesting that calcification morphology may provide structural information beyond total calcium burden.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.3,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Sexual Dimorphism and Genetic Variability in Cutaneous Microhemodynamics in BALB/c, C57BL/6J, and KM Mice.","authors":"Yingyu Wang, Xiang Xu, Weiqi Liu, Bing Wang, Yuan Li, Bingwei Li, Qin Ouyang, Xu Zhang, Mingming Liu, Ruijuan Xiu","doi":"10.1159/000551794","DOIUrl":"10.1159/000551794","url":null,"abstract":"<p><strong>Introduction: </strong>The comprehensive functionality of the cutaneous microcirculation extends beyond its barrier properties, implicating it in thermoregulation, nutrient exchange, and systemic disease pathophysiology. Our study aimed to investigate the sex- and strain-specific differences in cutaneous microvascular function and their relationships with systemic hemodynamics, skin physiology, and sex hormone-related metabolites in BALB/c, C57BL/6J, and KM mice.</p><p><strong>Methods: </strong>We analyzed sex- and strain-specific cutaneous microhemodynamics to elucidate the impact of genetic and hormonal factors on microvascular function. Our multidimensional approach incorporated body mass, glycemic levels, cardiovascular profiles, along with histological and immunohistochemical analyses, flagging discernible sexual dimorphism and inter-strain variability.</p><p><strong>Results: </strong>Immunohistochemical staining revealed significantly higher estrogen receptor expression within the male cutaneous microcirculation for BALB/c and C57BL/6J strains. Laser Doppler flowmetry and wavelet transform analysis revealed significant sex-specific and inter-strain differences in cutaneous microvascular blood perfusion, RBC concentration, and blood flow velocity. Sexual dimorphism was evident in the KM strain across all examined amplitudes, with males typically displaying greater variability. Similar wavelet statistical analysis results in microhemodynamic indicators suggest the homeostasis of specific frequency contributions. Furthermore, we observed significant correlations between systemic hemodynamics and cutaneous microcirculatory function, with heart rate and blood pressure variably associated with microvascular flow, erythrocyte concentration, and blood perfusion dynamics across strains and sexes.</p><p><strong>Conclusion: </strong>These findings indicate the complexities of cutaneous microhemodynamics, emphasizing the interplay between genetic lineage and sex-based differences.</p>","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"1-16"},"PeriodicalIF":1.3,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13446880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148330721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}