Neointimal Smooth Muscle Cells in Mouse Vein Grafts Are Not Recruited from the Adjacent Artery.

IF 1.8 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE
Klas Österberg, Joakim Håkansson, Erney Mattsson
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引用次数: 0

Abstract

Introduction: Smooth muscle cells (SMCs) with an origin separate from the local vein wall contribute to formation of intimal hyperplasia (IH) in mouse vein grafts. The recruitment pathway of these cells has not been defined, but circulating progenitor cells and cells from the surrounding tissue or adjacent artery to which the vein graft is anastomosed are potential sources. The aim of this study was to clarify if cells from the adjacent artery contribute to neointimal formation in vein grafts.

Methods: Aortic segments from donor SM22α-LacZ mice were anastomosed to vein segments from wild-type (WT) C57BL/6 mice ex vivo followed by implantation of the composite grafts to the right common carotid arteries of WT recipient mice. Six weeks after surgery, the composite grafts were harvested, and histology was analyzed in longitudinal sections. SMCs with origin in the SM22α-LacZ arterial segments were identified with X-gal staining.

Results: LacZ-positive cells were found in the medial layer of the SM22α-LacZ arterial segments but were not found in the IH in the vein graft segment.

Conclusion: SMCs in vein grafts are not recruited from the adjacent artery through migration across the anastomosis.

小鼠静脉移植物中的新生内膜平滑肌细胞不是来自邻近动脉。
来源与局部静脉壁分离的平滑肌细胞(SMCs)有助于小鼠静脉移植物内膜增生的形成。这些细胞的募集途径尚未明确,但循环祖细胞和来自静脉移植物吻合的周围组织或邻近动脉的细胞是潜在的来源。本研究的目的是澄清邻近动脉的细胞是否有助于静脉移植物中新内膜的形成。方法将供体SM22-LacZ小鼠主动脉段与野生型(WT) C57BL/6小鼠离体静脉段吻合,并将复合移植物植入WT受体小鼠右侧颈总动脉。术后6周,取复合移植物,纵切面进行组织学分析。通过X-gal染色确定起源于SM22-LacZ动脉段的SMCs。结果SM22-LacZ动脉段内侧可见lacz阳性细胞,静脉段内膜增生未见阳性细胞。结论静脉移植物的骨髓间充质干细胞不是通过吻合口迁移从邻近动脉获得的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Vascular Research
Journal of Vascular Research 医学-生理学
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.
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