Priyanka Böttger, Tilo Grosser, Waltraut Ibe, Jean-Paul Boissel, Stephan Lindemann, Karl Werdan, HansJörg Schwertz, Harald Darius, Michael Buerke
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引用次数: 0
Abstract
Vascular smooth muscle cell proliferation and vascular homeostasis is thought to be regulated by nitric oxide and prostaglandins. We examined the effect of exogenous linsidomine, a NO releasing compound, in a model of balloon injury iliac arteries of rabbits. On the cellular levels NO can exert effects like cellular survival, growth and proliferation inhibition. Smooth muscle cell (SMC) proliferation was quantified as change of intima-media-ratio. Linsidomine injection or its vehicle was performed with an infusion-balloon catheter directly into the vessel wall. Balloon angioplasty resulted in a significant increase of intima-media-ratio during three weeks. Linsidomine treatment decreased the intima media ratio significantly (0.65±0.05 vs 1,2±0.2 intima-media-ratio, p<0.05). However, control vessels had an intima media ratio of 0.15±0.02. This effect was similar to NOS-2 transfected vessels following angioplasty. In in vitro experiments linsidomine inhibited significantly and dose-dependently rabbit smooth muscle cell proliferation measured by BrdU incorporation. Further linsidomine increased rate of apoptotic SMC's, however this effect was p53 independent. Simultaneous angioplasty of the rabbit iliac artery and direct injection of linsidomine into the vessel wall seem to be an effective strategy to reduce neointima formation. Despite single administration, local application of linsidomine resulted in potent inhibition of intima proliferation.
期刊介绍:
The ''Journal of Vascular Research'' publishes original articles and reviews of scientific excellence in vascular and microvascular biology, physiology and pathophysiology. The scope of the journal covers a broad spectrum of vascular and lymphatic research, including vascular structure, vascular function, haemodynamics, mechanics, cell signalling, intercellular communication, growth and differentiation. JVR''s ''Vascular Update'' series regularly presents state-of-the-art reviews on hot topics in vascular biology. Manuscript processing times are, consistent with stringent review, kept as short as possible due to electronic submission. All articles are published online first, ensuring rapid publication. The ''Journal of Vascular Research'' is the official journal of the European Society for Microcirculation. A biennial prize is awarded to the authors of the best paper published in the journal over the previous two years, thus encouraging young scientists working in the exciting field of vascular biology to publish their findings.